Arhgap31 (NM_020260) Mouse Tagged ORF Clone

CAT#: MR220005

Arhgap31 (Myc-DDK-tagged) - Mouse Rho GTPase activating protein 31 (Arhgap31)

Product Images

Circular map for MR220005

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Arhgap31
• Synonyms: 5830477L08Rik; AU041750; Cdgap; D330026I07Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR220005 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGAACAAGGGTGCCAAGCAGAAGCTGAAACGAAAGGGAGCCGCCAGTGCGTTTGGATGTGACCTGA
  CGGAGTATCTGGAAAGTTCGGGACAGGATGTTCCATATGTGCTGAAGAGCTGTGCTGAATTCATAGAGAC
  TCACGGCATTGTGGATGGAATCTATCGCCTCTCGGGCATCACCTCAAACATCCAGCGGCTGAGGCAAGAG
  TTTGGCTCAGATCAATGCCCAGATCTGACAAGGGAAGTGTACCTTCAAGACATCCACTGTGTGGGCTCCT
  TGTGCAAGCTCTACTTCCGGGAGCTGCCCAACCCCCTCCTGACCTACGAGCTCTATGAGAAATTCACGGA
  AGCAGTGTCTCATCGCCCAGAAGAAGGCCAGCTGGCCAGAATCCAGAATGTTATCCTGGAGCTTCCCCCA
  CCCCATTATAGGACCTTGGAATACCTGATTCGACACCTGGCCCACATCGCCTCATTCAGCAGCAAGACCA
  ACATGCACGCCAGGAACCTGGCCCTGGTGTGGGCCCCAAACCTCCTCAGGAGTAAGAAAATCGAAGCCAC
  TATTTGCAATGGAGATGCGGCGTTTCTGGCAGTCCGGGTCCAGCAGGTGGTGATTGAGTTCATATTAAAT
  CACGCTGATCAAATCTTCAATGGCGGTGCTCCAGGGGCTCTGCAGCAGGATGAAAGCCGTACTATCACAA
  AGAGCCTGACCTTGCCAGCCTTGTCCCTGCCCATGAAGTTGGTAAGCCTGGAGGAGGCTCAGGCCCGAAG
  CTTGGCGACAAACCACCCTGCTCGGAAGGAAAGACGAGAGAACAGCCTGCCCGAGATCGTCCCTCCTCCC
  TTCCACACCGTCCTTGAGTTACCAGACAACAAGAGAAAGCTTTCCAGCAAATCAAAGAAGTGGAAATCGA
  TATTTAACCTGGGACGTTCTGGATCGGACTCGAAGTCCAAGCTGAGTAGAAACGGGAGTGTGTTCGTGAG
  AGGCCAGAGGCTGTCAGTGGAAAAGGCTACTATCCGGCCAGCAAAAAGCATGGACTCACTGTGTTCGGTG
  CCTGTGGAAGGGAAAGAAAACAAAGGAAATTTCAGTCGAACAGTCACCACTGGGGGATTTTTCATTCCAG
  CCACAAAAATGCACGCGAGCAGCACCGGAAGCTCCTGTGACCTCAGCAAGGAGGGTGAATGGGGTCAGGA
  GGGGATGCCCGCAGGGGCTGAGGGGGGCTGCGAGGTGGGCGGTCAGATCCGGCCACTGCCTGAGCAGCTG
  AAGGTGTTCCGCCCCATCGGAGACCCTGAGAGCGAGCAGTCCGCCCCAAAGCTTCTCGGGATGTTCTACA
  CCTCCAGCGACAGTCCTGGCAAATCTGTCTTCACAAGCAGCCTGTTCCAGATGGAACCCTCACCCCGCCA
  CCAGCGAAAGGCGCTCAACATCTCCGAGCCCTTCGCCGTGTCTGTGCCACTCCGCGTGTCCGCCGTCATC
  AGTACCAACAGCACCCCGTGCAGGACACCCCCCAAAGAGCTACAGTCTCTCTCCAGCCTCGAAGAGTTTT
  CTTTCCAAGGTTCAGAGAGTGGAGGATGGCCAGAAGAAGAGAAGCCCCTGGGGGCTGAGTCTTTTCCAGG
  CTCTGTTACTAAGAAGGCAGCTACCGAGGACACCAAGCCAGAACCTGAAGTCCCGGGGAGAGCAGAATGT
  AGCCAAAGCCCTCCCCTGGACCCAGGCACCCAAGTGGAGAAGAAAACCTTGCATGTATCCCTGGGCTCTC
  AAGTATCAAAGGAAGCAGAGAAGCGGCCAAAGGCTGAGAAGGTGATGGAGGAGAGCCAGGGTGCTAGCCA
  GCCGAAGCCCAGCACTCCACAAGAGAGCCTCGGGGCTGGGACTGAACCATTGATTCTCCACGAGATGGAT
  GAAGAAGACCTGGCTCAGGCCTTGATCTGGCCTGAGATTCAACAGGAACTGAAAATCATTGAATCAGAGG
  AGGAGTTCTCTTCCTTGCCACCCGCTGCTCAGAAGACAAGCCCGATTCCTGAGTCAAGCCCAGCCCCATT
  TCCCTTCCCAGAAGCCCCTGGGAGCTTGCCTAGTAGCTCTGCTCCTCGGGAGGTGTGGACTAGGGACGCA
  GCCAATCAGAGCATACAGGAAGCTGCTATCCTCACTGACAGAGAGAAGCTAGAGCCAGTCTGCAGCCTCC
  TCGAGTCAGAGTCTCAGCAGGAGCTCAGCCCAGACCCAGCCAGTCTAGCGCCTCTGGAAATGCTTCTGTT
  TGAGAAGGTATCTTCTCCGGCTAGGATAGAGATAGGAGGCCCAAGGAATCTCTCTCCCCCTCTTACTCCT
  GCTCCTCCTCCCCCCACACCTCTGGAGGAGGAGCCTGAAGTCCTGCTGTCAAAGGAAGGTCCTGACAGGG
  AAGATGCAGCCAGGGATTCCAGGACAGATGTCTACACAGAGCAGCCAACCCCCAAAGAGAGCCCTGGCAT
  CCCTACCCCCTGTCAGAGAGAGGAAGCTATTGCAAGCCCAAATGAGAAGCAAAACGCAAGGCATGCTGTT
  CCTGAGAACAAAGGCCCTGGTCTTCCAAGCCCAACCAAAGAGGTTGACATCATCCCACAAGAGGAGGGAG
  GTGCTCCGCATTCAGCACAAGAGCCTTCAGACTGTGACGAAGATGACACTGTGACGGACCCTGCCCAGCA
  TGGCCTGGAGATGGTGGAACCCTGGGAGGAACCCCAGTGGGTGACCAGTCCCCTTCATTCTCCCACCTTG
  AAAGAAGTTCAGGAGTCCCAGACGCAAGGCTCCCAGGGCCACCGATTAGAGAGGAGACTTTGCCACAGGC
  CCAGCCTTCGCCAGAGCCATTCTCTCGATAGTAAAACCACTGGAAACAGCCACTGGACTCTTGAGGCGCC
  CTTCTCCAGCAGTTGTGCCAATCTGGAAACAGAGAGGAACTATGAGCCTCTGCAGCCCCCGGCAGCCAGG
  ACCAAGATTGCCGGATTGGAAGAGAAAGCCCTAAAAGCCTTCAGAGAGTTCTCTGGCCTGAAAGGGTTAG
  AGGTTCTTCCCAGTCAGAAGGGACCATCTGGTATACAACCCAAACCAGTAGAAACCAACTTCATGGGTTT
  AGCAGAGGGAAAGGAGCAGGAACCACAACTGGAACTTAGCAACCGTCAGATGAAACACAGTGATGTTCCC
  GGGCCAGATAGCAGCAAGGAGAGTTCACCGAGGGCTCAAGACAGCACCTTACCTGGGGAACATCCCTTAC
  AGTTACAGCTTAAGAACACTGAGTGTGGGCCCTCAAAAGGGAAACACAGGCCGTCTTCCCTCAACCTGGA
  CTCTGCCACTCCCATAGCTGACCTTTTCCGACTTGAGAATGGGGCTCCCTTTAGCTCACCTGGAATAGAG
  CTCTCTGAACTAGGAGACACCAAGGTCACCTGGATGAGCTCCTCTCACTGCAAAGCAGCCCCCTGGAATT
  CCCAGGACACGCAGGACCTGGACATTGTTGCCCACACCCTGACAGGCCGCAGGAATTCGGCTCCTGTAAG
  TGTGTCAGCTGTGAGAACCTCCTTCATGGTCAAAATGTGTCAGGCCAAGGCAGTCCCTGTCATCCCACCC
  AAGATCCAATACACACAGATCCCACAGCCCCTGCCCTCTCAGAGCACAGGGGAGGGAGGGGCTCAGCCTC
  TGGAGAGAAGCCAGGAAGAACCTGGTTCAACCCCTGAGATCCCTCAGAAATCCACCAAAGATGATTCTCC
  CTCCTCCCTGGGAAGCCCAGAGGAAGAGCAACCAAAGCAAGAGACAGGAGCCTCCGCATCCCGGAGGCAG
  GCCAGCATTACATCCTGCATGTATGAGGGGTCCTCCTGCTCTCCAGAACCAAGTGCATCCACTCTGGCCT
  CCACACAGGATGCGGTGGTGCAATGCAGAAAACGCACATCAGAGACAGAGCCATCAGGAGACAACCTTCT
  TTCTTCCAAACTGGAGCGAGCATCTGGGGGTCCTAAAGCTTTCCACAGGTCAAGGCCAGGAAGACCTCAG
  AGCCTAATCTTATTCCCTATCATGGACCATCTACCTTCCTCACCCACAGTGATAGATTCCAAGGTCCTAC
  TGTCCCCTATCAGGAGCCCTACTCAGACAGTTTCCCCTGGTCTTCTCTGTGGAGAGTTGGCCGAAAACAC
  ATGGATCACACCAGAAGGGGTCACGCTTAGGAATAAAATGACCATCCCTAAGAATGGCCAAAGACTCGAG
  ACTTCGACAAGCTGTTTTTACCAGCCCCAGCGGAGATCCGTGATTCTGGATGGAAGAAGTGGGAGACAGA
  TAGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR220005 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MKNKGAKQKLKRKGAASAFGCDLTEYLESSGQDVPYVLKSCAEFIETHGIVDGIYRLSGITSNIQRLRQE
  FGSDQCPDLTREVYLQDIHCVGSLCKLYFRELPNPLLTYELYEKFTEAVSHRPEEGQLARIQNVILELPP
  PHYRTLEYLIRHLAHIASFSSKTNMHARNLALVWAPNLLRSKKIEATICNGDAAFLAVRVQQVVIEFILN
  HADQIFNGGAPGALQQDESRTITKSLTLPALSLPMKLVSLEEAQARSLATNHPARKERRENSLPEIVPPP
  FHTVLELPDNKRKLSSKSKKWKSIFNLGRSGSDSKSKLSRNGSVFVRGQRLSVEKATIRPAKSMDSLCSV
  PVEGKENKGNFSRTVTTGGFFIPATKMHASSTGSSCDLSKEGEWGQEGMPAGAEGGCEVGGQIRPLPEQL
  KVFRPIGDPESEQSAPKLLGMFYTSSDSPGKSVFTSSLFQMEPSPRHQRKALNISEPFAVSVPLRVSAVI
  STNSTPCRTPPKELQSLSSLEEFSFQGSESGGWPEEEKPLGAESFPGSVTKKAATEDTKPEPEVPGRAEC
  SQSPPLDPGTQVEKKTLHVSLGSQVSKEAEKRPKAEKVMEESQGASQPKPSTPQESLGAGTEPLILHEMD
  EEDLAQALIWPEIQQELKIIESEEEFSSLPPAAQKTSPIPESSPAPFPFPEAPGSLPSSSAPREVWTRDA
  ANQSIQEAAILTDREKLEPVCSLLESESQQELSPDPASLAPLEMLLFEKVSSPARIEIGGPRNLSPPLTP
  APPPPTPLEEEPEVLLSKEGPDREDAARDSRTDVYTEQPTPKESPGIPTPCQREEAIASPNEKQNARHAV
  PENKGPGLPSPTKEVDIIPQEEGGAPHSAQEPSDCDEDDTVTDPAQHGLEMVEPWEEPQWVTSPLHSPTL
  KEVQESQTQGSQGHRLERRLCHRPSLRQSHSLDSKTTGNSHWTLEAPFSSSCANLETERNYEPLQPPAAR
  TKIAGLEEKALKAFREFSGLKGLEVLPSQKGPSGIQPKPVETNFMGLAEGKEQEPQLELSNRQMKHSDVP
  GPDSSKESSPRAQDSTLPGEHPLQLQLKNTECGPSKGKHRPSSLNLDSATPIADLFRLENGAPFSSPGIE
  LSELGDTKVTWMSSSHCKAAPWNSQDTQDLDIVAHTLTGRRNSAPVSVSAVRTSFMVKMCQAKAVPVIPP
  KIQYTQIPQPLPSQSTGEGGAQPLERSQEEPGSTPEIPQKSTKDDSPSSLGSPEEEQPKQETGASASRRQ
  ASITSCMYEGSSCSPEPSASTLASTQDAVVQCRKRTSETEPSGDNLLSSKLERASGGPKAFHRSRPGRPQ
  SLILFPIMDHLPSSPTVIDSKVLLSPIRSPTQTVSPGLLCGELAENTWITPEGVTLRNKMTIPKNGQRLE
  TSTSCFYQPQRRSVILDGRSGRQIE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020260
• ORF Size: 4278 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020260.2, NP_064656.2
• RefSeq Size: 7722 bp
• RefSeq ORF: 4278 bp
• Locus ID: 12549
• UniProt ID: A6X8Z5
• Cytogenetics: 16 B4
• MW: 155.3 kDa
• Gene Summary: Functions as a GTPase-activating protein (GAP) for RAC1 and CDC42. Required for cell spreading, polarized lamellipodia formation and cell migration.[UniProtKB/Swiss-Prot Function]