Kcnt1 (NM_001145403) Mouse Tagged ORF Clone

CAT#: MR219855

Kcnt1 (Myc-DDK-tagged) - Mouse potassium channel, subfamily T, member 1 (Kcnt1), transcript variant 2

Product Images

Circular map for MR219855

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Kcnt1
• Synonyms: C030030G16Rik; s; Sl; Slack; slo2; Slo2.2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR219855 representing NM_001145403
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACGACCTGGACACGGAGGTGCTGCCCTTGCCGCCAAGGTACCGATTCCGTGACCTGCTTTTAGGAG
  ACCAGCCATTCCCGAACGACGATAGGCTTCATGTGGAAGATTTCAGCCTGGACTCTTCCCTTTCTCAGGT
  CCAGGTGGAGTTCTATGTCAATGAGAACACCTTCAAAGAGCGGCTCAAGCTGTTCTTCATCAAAAACCAA
  AGATCCAGCCTGAGGATCCGGCTGTTCAACTTCTCCCTCAAGCTCCTCACCTGCCTGCTGTATATCGTCC
  GTGTCCTGCTTGACAACCCAGACCAGGGCATCGGATGCTGGGGCTGCACGAAGTATAACTACACTTTCAA
  TGGCTCATCCTCTGAGTTCCACTGGGCTCCCATCCTGTGGGTGGAGAGGAAAATGGCTCTGTGGGTGATC
  CAGGTCATTGTGGCCACAATAAGCTTCTTAGAGACCATGCTCATCATTTACCTCAGCTACAAAGGCAACA
  TCTGGGAGCAGATATTCCATGTGTCTTTCGTCTTGGAGATGATCAACACACTGCCCTTCATCATCACGGT
  CTTCTGGCCACCTCTGCGGAACCTGTTCATCCCCGTGTTTCTCAACTGCTGGCTGGCCAAGCATGCGCTG
  GAGAACATGATTAATGACTTCCACCGTGCCATCCTACGCACACAGTCAGCCATGTTCAACCAGGTGCTCA
  TCCTGTTCTGCACCCTGCTGTGCCTGGTCTTCACAGGGACCTGTGGGATCCAGCACCTGGAGCGAGCAGG
  TGGCAACTTGAACCTGCTGACCTCCTTCTACTTCTGCATCGTGACTTTCTCAACTGTGGGCTTCGGTGAT
  GTGACGCCCAAGATCTGGCCATCCCAGCTCCTGGTGGTCATCCTGATCTGTGTCACCCTTGTGGTGCTCC
  CACTGCAATTTGAAGAGCTTGTCTACCTCTGGATGGAGCGTCAGAAGTCAGGGGGCAACTATAGCCGCCA
  CCGAGCACGGACGGAGAAGCACGTAGTCCTGTGTGTGAGCTCCCTCAAGATTGATCTCCTCATGGACTTC
  CTGAATGAGTTCTATGCCCATCCCCGGCTCCAGGACTACTACGTGGTCATCCTGTGTCCCTCTGAAATGG
  ACGTCCAGGTGCGCAGGGTGCTGCAGATTCCCCTGTGGTCCCAGCGGGTCATCTACCTCCAGGGCTCTGC
  CCTCAAGGACCAGGATCTCATGCGAGCCAAGATGGACAACGGAGAGGCCTGCTTTATCCTCAGCAGCAGG
  AATGAGGTGGACCGCACAGCTGCGGATCACCAGACCATCCTTCGAGCCTGGGCTGTGAAGGACTTTGCCC
  CCAACTGTCCCCTCTATGTCCAGATCCTCAAGCCCGAAAACAAGTTTCACGTCAAATTTGCTGACCACGT
  GGTATGCGAGGAAGAGTGCAAGTACGCCATGCTGGCCCTGAACTGCATCTGCCCGGCCACCTCCACCCTC
  ATCACCCTGCTGGTGCACACGTCCCGTGGCCAGGAAGGACAGGAGTCTCCCGAGCAGTGGCAGCGCACGT
  ACGGGAGGTGCTCGGGCAACGAGGTGTACCACATTCGCATGGGTGACAGTAAATTCTTCCGGGAGTATGA
  GGGCAAGAGCTTCACCTACGCAGCCTTCCACGCGCACAAGAAATATGGGGTGTGCCTCATCGGGCTGAAG
  CGTGAGGAGAACAAGAGTATCCTGCTGAACCCAGGACCACGGCACATCCTGGCTGCCTCCGACACCTGCT
  TCTATATCAATATTACCAAGGAGGAGAACTCAGCTTTCATCTTCAAACAGGAGGAGAAGCAGAAGAGACG
  GGGCCTTGCAGGGCAGGCACTATATGAAGGGCCCTCCCGGCTCCCAGTGCATAGCATCATCGCCTCTATG
  GGGACAGTGGCCATGGACCTGCAGAACACAGATTGCCGGCCCTCCCAGGGTGGCAGTGGCGGGGACGGCA
  CAAAGCTGACTCTGCCCACCGAGAACGGCTCTGGTAGTCGACGCCCCAGCATCGCACCCGTTCTGGAGTT
  GGCAGACAGCTCAGCCCTGTTGCCCTGCGACCTGCTGAGTGACCAGTCAGAGGATGAGGTGACACCCTCG
  GACGACGAGGGGCTCTCTGTGGTTGAGTACGTGAAGGGCTATCCCCCCAACTCACCCTACATTGGCAGCT
  CCCCGACTTTATGCCACCTCCTGCCTGTGAAAGCCCCCTTCTGCTGCCTGCGGTTGGACAAGGGCTGCAA
  ACACAACAGCTACGAGGATGCCAAGGCCTATGGGTTCAAGAACAAGCTGATTATTGTCTCTGCTGAGACG
  GCAGGCAACGGGCTCTACAACTTCATCGTGCCTCTGCGCGCCTACTACCGGTCCCGCCGGGAGCTCAACC
  CTATCGTGTTGCTGCTTGACAACAAGCCTGACCACCACTTCCTGGAGGCCATCTGTTGCTTCCCCATGGT
  CTACTACATGGAGGGATCCGTGGACAACCTGGACAGCTTACTGCAGTGTGGCATCATCTATGCTGACAAC
  CTGGTGGTGGTGGACAAGGAGAGCACTATGAGCGCTGAAGAGGACTACATGGCAGATGCCAAGACCATCG
  TCAATGTGCAAACCATGTTCCGGCTTTTCCCCAGTCTCAGCATCACCACGGAGCTCACACACCCTTCCAA
  CATGCGGTTCATGCAGTTCCGTGCCAAGGACAGCTACTCTCTGGCTCTTTCCAAACTTGAAAAGCAAGAA
  CGGGAGAACGGCTCCAACCTGGCCTTCATGTTCCGCCTGCCATTTGCTGCTGGTCGAGTATTTAGTATCA
  GCATGTTGGATACACTGCTCTACCAGTCCTTCGTGAAGGACTACATGATCACCATCACCAGGCTGCTCTT
  GGGCCTTGATACTACACCAGGCTCCGGCTACCTCTGTGCAATGAAGGTAACCGAGGACGACCTGTGGATC
  CGCACTTACGGCCGCCTCTTCCAGAAACTCTGCTCCTCCAGCGCCGAGATCCCCATCGGCATCTACAGGA
  CCGAGTGCCATGTCTTCTCGGAGCCCCATGACGTCAGAGCCCAGTCTCAGATCTCGGTGAACATGGAGGA
  CTGCGAGGATACTCGGGAGGCCAAGGGACCCTGGGGCACACGAGCTGCATCTGGCAGTGGCAGCACCCAT
  GGCCGTCACGGGGGCAGTGCTGACCCAGTGGAGCACCCACTACTACGTCGCAAGAGCCTGCAGTGGGCCC
  GCAAGCTGAGTCGCAAGAGCACCAAGCAGGCAGGGAAGGCACCTGTGGCCACAGACTGGATCACCCAGCA
  GCGGCTCAGCCTGTACCGACGCTCAGAGCGCCAGGAGCTCTCAGAGCTGGTCAAGAACCGAATGAAGCAC
  CTGGGACTGCCCACCACTGGCTATGAGGACGTAGCAAATTTAACAGCCAGTGATGTGATGAATCGGGTAA
  ACCTGGGATATTTGCAAGATGAGATGAACGATCATCACCAGAACACCCTTTCCTATGTACTCATCAACCC
  CCCGCCAGACACAAGACTGGAACCCAACGACATTGTGTACCTCATCCGTTCCGACCCCCTGGCCCATGTG
  GCCAGCAGCTCCCAGAGTCGGAAAAGCAGCTGCAGCAACAAGCTCTCATCCTGTAATCCTGAGACCAGGG
  ATGAGACCCAGCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR219855 representing NM_001145403
  Red=Cloning site Green=Tags(s)
  
  MNDLDTEVLPLPPRYRFRDLLLGDQPFPNDDRLHVEDFSLDSSLSQVQVEFYVNENTFKERLKLFFIKNQ
  RSSLRIRLFNFSLKLLTCLLYIVRVLLDNPDQGIGCWGCTKYNYTFNGSSSEFHWAPILWVERKMALWVI
  QVIVATISFLETMLIIYLSYKGNIWEQIFHVSFVLEMINTLPFIITVFWPPLRNLFIPVFLNCWLAKHAL
  ENMINDFHRAILRTQSAMFNQVLILFCTLLCLVFTGTCGIQHLERAGGNLNLLTSFYFCIVTFSTVGFGD
  VTPKIWPSQLLVVILICVTLVVLPLQFEELVYLWMERQKSGGNYSRHRARTEKHVVLCVSSLKIDLLMDF
  LNEFYAHPRLQDYYVVILCPSEMDVQVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKMDNGEACFILSSR
  NEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFADHVVCEEECKYAMLALNCICPATSTL
  ITLLVHTSRGQEGQESPEQWQRTYGRCSGNEVYHIRMGDSKFFREYEGKSFTYAAFHAHKKYGVCLIGLK
  REENKSILLNPGPRHILAASDTCFYINITKEENSAFIFKQEEKQKRRGLAGQALYEGPSRLPVHSIIASM
  GTVAMDLQNTDCRPSQGGSGGDGTKLTLPTENGSGSRRPSIAPVLELADSSALLPCDLLSDQSEDEVTPS
  DDEGLSVVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLIIVSAET
  AGNGLYNFIVPLRAYYRSRRELNPIVLLLDNKPDHHFLEAICCFPMVYYMEGSVDNLDSLLQCGIIYADN
  LVVVDKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRFMQFRAKDSYSLALSKLEKQE
  RENGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMITITRLLLGLDTTPGSGYLCAMKVTEDDLWI
  RTYGRLFQKLCSSSAEIPIGIYRTECHVFSEPHDVRAQSQISVNMEDCEDTREAKGPWGTRAASGSGSTH
  GRHGGSADPVEHPLLRRKSLQWARKLSRKSTKQAGKAPVATDWITQQRLSLYRRSERQELSELVKNRMKH
  LGLPTTGYEDVANLTASDVMNRVNLGYLQDEMNDHHQNTLSYVLINPPPDTRLEPNDIVYLIRSDPLAHV
  ASSSQSRKSSCSNKLSSCNPETRDETQL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001145403
• ORF Size: 3654 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001145403.2
• RefSeq Size: 3771 bp
• RefSeq ORF: 3657 bp
• Locus ID: 227632
• Cytogenetics: 2 A3
• MW: 138.9 kDa
• Gene Summary: This gene encodes a member of the Slo potassium channel family that has shown to be activated by both sodium and chloride ions. This channel represents the largest potassium channel subunit yet identified. This channel may be important in development and pain signaling. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]