Cbfa2t3 (NM_009824) Mouse Tagged ORF Clone

CAT#: MR219692

Cbfa2t3 (Myc-DDK-tagged) - Mouse core-binding factor, runt domain, alpha subunit 2, translocated to, 3 (human) (Cbfa2t3), transcript variant 1

Product Images

Circular map for MR219692

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cbfa2t3
• Synonyms: A630044F12Rik; AI465270; AW229127; Cbfa2t3h; ETO-2; Eto2; MTGR2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR219692 representing NM_009824
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCCAGGCATCCACCACCACTCTGGAGAGTGGGGCACTCCTCTCGGGACCCCGGGGTCTTCAGAATG
  GAAGCCCAGCTCACAGGAAGGAGAAGGCTGCAGCAATGCCAGACTCCCCGGCTGAAGTGAAGACGCAGCC
  CCGGTCCACACCCCCCAGCATGCCGCCCCCACCTCCCACCTCATCCCAGGGAGCCACACGACCACCCTCC
  TTCACACCTCACACACATGGCGAGGATGGACCTGCGACGTCTCTTCCCCATGGCCGTTTCCACGGCTGCT
  TAAAGTGGTCCATGGTCTGTCTCTTGATGAACGGCAGCAGCCACTCACCCACGGCCATCCACGGTGCCCC
  ATCTACACCCAATGGCTTCAGCAACGGCCCAGCCACCTCATCCACGGCCTCGCTCTCCACACAGCACCTG
  CCCCCGGCGTGCGGGGCACGGCAGCTCAGCAAGCTTAAGCGTTTCCTCACCACCCTACAGCAGTTTGGCA
  GTGACATCTCACCTGAGATCGGGGAGCGCGTGCGCACACTGGTGCTGGGCCTGGTGAACTCAACTCTGAC
  GATCGAAGAGTTTCATGCCAAGCTCCAGGAAGCCACCAACTTTCCACTGAGGCCGTTTGTTATCCCTTTT
  CTGAAGGCTAATCTTCCACTGCTGCAGCGTGAGCTCCTGCACTGTGCCCGCCTGGCCAAACAGACACCTG
  CCCAGTACCTGGCCCAGCACGAACAGCTGCTGCTGGACGCCAGCGCCACCTCCCCTGTCGACTCGTCTGA
  GCTCCTGCTGGAAGTCAACGAGAACGGCAAAAGGAGAACACCTGACAGGACCAAAGAGAATGGATCAGAC
  CGGGACCCTCTGCACCCCGACCACCTCAGTAAGCGGTCCTGCACCCTGAGCCCCGCCCAGCGCTGCAGCC
  CCAGCAATGGGCTGCCCCACCCGACGCCACCCCCACCCCCGCACTATCGCCTGGAGGACATGGCCATGGC
  CCACCATTTCCGGGACTCCTACCGCCATCCTGATCCCCGAGAGCTACGGGAACGCCACCGGCCCCTGGCC
  ATACCTGGGTCTCGACAAGAAGAAGTGATTGATCACAGGCTCACAGAACGCGAGTGGGCAGAAGAATGGA
  AGCACCTCAACAGTCTTCTGAACTGCATCATGGACATGGTGGAGAAGACCCGGCGATCCCTCACCGTCCT
  GCGCCGGTGTCAGGAGGCCGACCGTGAGGAACTCAACCACTGGATCCGGTGCTACAGTGACTCTGAGGAG
  GGGAAGAAGGGCCCTACCCCCATCTCTGCCCGGTCCCTCAACAGCTGCAGTGGCCCTGAGGGGTCTCAGC
  TAGATGTTCACCGGGACTTCACGCCCAGGACCCTGTCTGGCTACATGCCTGAAGAGATCTGGAGGAAGGC
  TGAAGAAGCTGTGAATGAGGTGAAGCGCCAGGCCATGTCAGAACTACAGAAAGCTGTGTCTGATGCGGAG
  CGCAAAGCCCATGAACTCATCACCACAGAGCGTGCCAAGATGGAACGAGCCCTGGCGGAGGCCAAGCGAC
  AGGCCTCGGAGGATGCCCTGACTGTCATCAACCAGCAAGAGGACTCCAGCGAGAGCTGCTGGAACTGCGG
  GCGCAAGGCCAGCGAGACGTGCAGTGGCTGTAACGCCGCCCGCTACTGCGGGTCCTTCTGTCAGCACAAA
  GACTGGGAGAAACACCATCACGTGTGCGGCCAGAGTCTGCAGGGCCCCGCGGCTGCAGTGGCTGACCCAC
  TACCTGGACAGCCTGACGCCACTGCCAGCCCCAGCGAAGCCGGCTCGGCAGGGCCCTCTCGTCCCTGCTC
  TCCGGGGCCGCCAGGCCCGCTGGACGCTGCTGTGCCCCGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR219692 representing NM_009824
  Red=Cloning site Green=Tags(s)
  
  MSQASTTTLESGALLSGPRGLQNGSPAHRKEKAAAMPDSPAEVKTQPRSTPPSMPPPPPTSSQGATRPPS
  FTPHTHGEDGPATSLPHGRFHGCLKWSMVCLLMNGSSHSPTAIHGAPSTPNGFSNGPATSSTASLSTQHL
  PPACGARQLSKLKRFLTTLQQFGSDISPEIGERVRTLVLGLVNSTLTIEEFHAKLQEATNFPLRPFVIPF
  LKANLPLLQRELLHCARLAKQTPAQYLAQHEQLLLDASATSPVDSSELLLEVNENGKRRTPDRTKENGSD
  RDPLHPDHLSKRSCTLSPAQRCSPSNGLPHPTPPPPPHYRLEDMAMAHHFRDSYRHPDPRELRERHRPLA
  IPGSRQEEVIDHRLTEREWAEEWKHLNSLLNCIMDMVEKTRRSLTVLRRCQEADREELNHWIRCYSDSEE
  GKKGPTPISARSLNSCSGPEGSQLDVHRDFTPRTLSGYMPEEIWRKAEEAVNEVKRQAMSELQKAVSDAE
  RKAHELITTERAKMERALAEAKRQASEDALTVINQQEDSSESCWNCGRKASETCSGCNAARYCGSFCQHK
  DWEKHHHVCGQSLQGPAAAVADPLPGQPDATASPSEAGSAGPSRPCSPGPPGPLDAAVPR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009824
• ORF Size: 1860 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009824.2, NP_033954.2
• RefSeq Size: 7659 bp
• RefSeq ORF: 1863 bp
• Locus ID: 12398
• UniProt ID: O54972
• Cytogenetics: 8 E1
• MW: 68.4 kDa
• Gene Summary: Transcriptional corepressor which facilitates transcriptional repression via its association with DNA-binding transcription factors and recruitment of other corepressors and histone-modifying enzymes. Can repress the expression of MMP7 in a ZBTB33-dependent manner. Reduces the protein levels and stability of the transcriptinal regulator HIF1A; interacts with EGLN1 and promotes the HIF1A prolyl hydroxylation-dependent ubiquitination and proteasomal degradation pathway. Contributes to inhibition of glycolysis and stimulation of mitochondrial respiration by down-regulating the expression of glycolytic genes including PFKFB3, PFKFB4, PDK1, PFKP, LDHA and HK1 which are direct targets of HIF1A (By similarity). Regulates the proliferation and the differentiation of erythroid progenitors by repressing the expression of TAL1 target genes (PubMed:16407974). Plays a role in granulocyte differentiation (PubMed:15231665).[UniProtKB/Swiss-Prot Function]