Zdhhc8 (NM_172151) Mouse Tagged ORF Clone

CAT#: MR219503

Zdhhc8 (Myc-DDK-tagged) - Mouse zinc finger, DHHC domain containing 8 (Zdhhc8)

Product Images

Circular map for MR219503

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Zdhhc8
• Synonyms: D16H22S1738E; DHHC-8; E330009O14Rik; Op53c05; ZDHHCL1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR219503 representing NM_172151
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCCGCAGCCCCGGGACGCGCCTCAAACCCGCCAAGTACATCCCGGTGGCCACGGCCGCCGCGCTGT
  TGGTTGGTTCCAGCACACTCTTCTTCGTATTCACGTGCCCATGGTTGACAAGAGCTGTGTCTCCAGCTAT
  TCCTGTCTACAATGGCATCCTCTTCCTCTTTGTCCTGGCCAACTTCAGTATGGCTACCTTCATGGACCCT
  GGAGTCTTCCCCCGAGCGGACGAGGACGAGGACAAGGAGGATGACTTCCGGGCCCCACTGTACAAGAATG
  TGGATGTGCGGGGCATCCAGGTCCGCATGAAGTGGTGTGCAACGTGCCACTTTTACCGTCCACCGCGCTG
  CTCACACTGCAGTGTCTGTGACAACTGTGTGGAGGACTTTGACCATCACTGCCCCTGGGTCAACAACTGC
  ATTGGACGCCGCAACTACCGTTACTTCTTCCTGTTCCTGCTGTCACTCAGCGCGCACATGGTGGGGGTGG
  TGGCCTTCGGCCTGCTCTATGTGCTCAATCACTCGGAGGGGCTGGGAGCCGCCCACACCACCATCACCAT
  GGCTGTCATGTGTGTGGCTGGCCTTTTCTTCATTCCTGTCATCGGCCTCACTGGCTTTCACGTGGTACTG
  GTCACGCGGGGCCGCACCACCAATGAGCAGGTGACTGGGAAGTTCCGCGGGGGTGTGAATCCCTTCACCC
  GAGGCTGCTATGGGAACGTGGAGCACGTGTTATGCAGTCCCCTGGCGCCCCGGTATGTGGTGGAACCCCC
  CAGGATGCCGCTCTCAGTGAGCCTCAAGCCACCCTTCCTGAGACCTGAGCTCCTGGAACGAGCTGTGCCC
  CTCAAGGTCAAACTTAGCGACAATGGGCTGAAAGCTGGCCGCAGCAAGTCCAAGGGCAGTCTAGACCAGC
  TGGATGAGAAACCTCTGGACCTGGGACCTCCACTGCCCCCCAAGATAGAGGCTGGTACCTTTGGAAGAGA
  TCTGAAGACCCCAAGACCTGGCAGTGCTGAGAGTGCCCTATCAGTACAGAGGACCAGCCCCCCAACACCT
  GCCATGTATAAGTTCCGGCCAGCCTTCTCCACTGGTCCCAAGACACCCTTTTGTGGACCTAATGAGCAGG
  TCCCAGGTCCTGACTCCCTTACTCTGGCAGATGACAGCACCCACAGTCTAGACTTTGTGTCTGAGCCCAG
  CCTGGATCTCCCAGACCACGGGCCGGGTGGTCTGCGTCCTCCCTACCCGCCCTCCCCACCCCTCAACACC
  ACTGATGCCTTCTCAGGTGCCTTGCGCTCCCTGAGTCTCAAGGCTGCCAGTCGGAGGGGTGGGGACCACA
  TGACCTTACAGCCACTGCGCTCTGAAGGTGGGCCCCCTACACCTCACCGTAGTCTCTTTGCTCCTCATGC
  ACTGCCCAATCGAAATGGCAGCCTGTCATATGACAGCCTACTTAACCCTGGCTCACCCAGTGGCCACGCA
  TGCCCCACACACCCCTCTGTTGGTATAGCCAGCTACCATTCACCCTACCTGCACCCTGGGCCATCAGATC
  CACCGCGGCCCCCACCCCGCAGCTTCAGCCCTGTGCTGGGTCCCCGGCCTAGGGAACCCTCTCCTGTGCG
  CTATGACAACCTGTCTCGGACCATCATGGCCTCTATCCAGGAGCGCAAGGACAGGGAAGAGCGTGAACGG
  CTGCTGCGCTCCCAGACTGACTCACTCTTTGGCGACTCTGGTGTCTATGATACACCCAGCTCCTACAGCC
  TGCAACAGGCCAGTGTGTTAACGGAAGGCCCCCGTGGCTCTGTCCTGCGTTACGGCTCCAGGGATGACCT
  CGTGGCCGGGCCTGGCTTTGGTGGTGCCCGCAATCCTGCCCTGCAGACGTCATTGTCCTCGCTGTCCAGC
  TCCATGAGTCGGGCACCTCGGACATCTTCCTCCTCCCTGCAGGCTGACCAGGCCAACAACAATGCCCCAG
  GACCCCGGCCTGGCAGTGGTTCACATAGGTCACCTGCCCGTCAGGGCCTGCCTTCCCCACCAGGCACCCC
  CCGATCGCCCTCCTACACGGGCTCCAAGGCCGTCGCCTTCATCCACACAGACCTCCCGGACCGGCAGCCC
  TCACTGGCTATGCAGAGGGATCACCCTCAGCTGAAGACCCCCCCAAGTAAGCTTAACGGGCAGTCCCCAG
  GCATGGCCCGTCTGGGGCCTGCTGCCAGCCCCATGGGGCCCAACGCCAGCCCTGCCCGGCACACGCTGGT
  TAAGAAGGTGTCCGGCGTGGGTGGGACTACGTATGAAATCTCGGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR219503 representing NM_172151
  Red=Cloning site Green=Tags(s)
  
  MPRSPGTRLKPAKYIPVATAAALLVGSSTLFFVFTCPWLTRAVSPAIPVYNGILFLFVLANFSMATFMDP
  GVFPRADEDEDKEDDFRAPLYKNVDVRGIQVRMKWCATCHFYRPPRCSHCSVCDNCVEDFDHHCPWVNNC
  IGRRNYRYFFLFLLSLSAHMVGVVAFGLLYVLNHSEGLGAAHTTITMAVMCVAGLFFIPVIGLTGFHVVL
  VTRGRTTNEQVTGKFRGGVNPFTRGCYGNVEHVLCSPLAPRYVVEPPRMPLSVSLKPPFLRPELLERAVP
  LKVKLSDNGLKAGRSKSKGSLDQLDEKPLDLGPPLPPKIEAGTFGRDLKTPRPGSAESALSVQRTSPPTP
  AMYKFRPAFSTGPKTPFCGPNEQVPGPDSLTLADDSTHSLDFVSEPSLDLPDHGPGGLRPPYPPSPPLNT
  TDAFSGALRSLSLKAASRRGGDHMTLQPLRSEGGPPTPHRSLFAPHALPNRNGSLSYDSLLNPGSPSGHA
  CPTHPSVGIASYHSPYLHPGPSDPPRPPPRSFSPVLGPRPREPSPVRYDNLSRTIMASIQERKDREERER
  LLRSQTDSLFGDSGVYDTPSSYSLQQASVLTEGPRGSVLRYGSRDDLVAGPGFGGARNPALQTSLSSLSS
  SMSRAPRTSSSSLQADQANNNAPGPRPGSGSHRSPARQGLPSPPGTPRSPSYTGSKAVAFIHTDLPDRQP
  SLAMQRDHPQLKTPPSKLNGQSPGMARLGPAASPMGPNASPARHTLVKKVSGVGGTTYEISV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_172151
• ORF Size: 2286 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172151.4, NP_742163.4
• RefSeq Size: 4887 bp
• RefSeq ORF: 2289 bp
• Locus ID: 27801
• UniProt ID: Q5Y5T5
• Cytogenetics: 16 11.29 cM
• MW: 82.5 kDa
• Gene Summary: Palmitoyltransferase involved in glutamatergic transmission. Mediates palmitoylation of ABCA1.[UniProtKB/Swiss-Prot Function]