Ahi1 (NM_026203) Mouse Tagged ORF Clone

CAT#: MR219438

Ahi1 (Myc-DDK-tagged) - Mouse Abelson helper integration site 1 (Ahi1), transcript variant 1

Product Images

Circular map for MR219438

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ahi1
• Synonyms: 1700015F03Rik; Ahi-1; D10Bwg0629e
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR219438 representing NM_026203
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCCAGAAACTCCAGAGAAGGTGGATTCTGCACAAGAGAAAGTCAGAGGCAAGACCCCGACAGCAG
  ATGATTCAGATGACAGCCGAGAAAAGACTGGCATAGAAGAGAAAGGAGAACTGACCGACGCCTATCAGCT
  GCAGGTAGCTGAAGAAATGGCAAAGGAGATCAAGAAGAAAATAAGAAAGAAGCTAAAGGAACAGCTGACC
  TACTTTCCTCCAGACACTCTATTGCATGACGACAAGCTGGCCAGTGAAAAAAGAAAGAAGAAGAAGAAGA
  AAGTGCCAGTGCCCACTAAGCCTGAGTCAAGTCCCTCAGATGTCTGTGACAGTGCAGTTGAAGGGGAACA
  AAAGAAAGAAGGTACCCCTGAGGACTCTCAGCACATGGAGGGAATCTGCTCGAGAGAGCAGGATGTGGAT
  GCCACTGTGCCAGAGAACGCAAAGCCCAAACCCAAGAAGACAAAGAAGAAGACTAAAGCAGTTTCAAATG
  ATAATGAAGACACTAATGGAGATGGTGTTCATGAGATAACAAGCCGAGACAGCCCAGTTCATCCCAAGTG
  CTTGCTGGATGATGACCTCGTCATGGGAGTCTACATTCACCGAACCGATAGACTTAAATCTGACTTTATG
  ATTTCTCACCCAATGGTAAAGATCCATGTGGTTGATGAGCACACTGGCCAGTACGTCAAGAAAGATGACA
  GTGAACGTCCTGTTTCATCTTATTATGAGAAAGACAACGTGGACTATATTCTCCCTATTATGACACAGCC
  ATATGACTTTAAAAAGCTAAAGTCAAGGCTTCCGGAGTGGGAGGAGCAAGTTATTTTTAATGAAAATTTT
  CCCTATTTGCTTCGAGAGTTTGAAGAATGTCCAAAAGTCATCCTGTTCTTTGAGATTCTTGACTTTTTAA
  GCATGGATGAAATCAAGAATAACTCTGAGGTTCAAAACCAAGAGTGTGGCTTTCGGAAAATTGCCTGGGC
  GTTTCTTAAGCTTCTGGGAGCCAATGGGAATGCAAACATCAACTCAAAACTTCGCCTGCAGCTCTACTAC
  CCACCGACTAAGCCTCGATCCCAGCTAAATGTCGTCGAGGTTTTTGAATGGTGGTCCAAGTGTCCAAGAA
  ATCGTTATCCATCAACATTGTATGTAACCGTACGAGGATTGAAAGTCCCGGACTGTATAAAGCCATCTTA
  CCGCTCTATGATGGCTCTCCAGGAGGAAAGGGGTACACCAGTGTACTGTGAACGTCACCGTGAAACAAGT
  TCCGTGGACACAGAACCTGGACTAGAAGATTCAAAGGAGGAAGTGAAGTGGAAACGTCTGCCTGGCCAGG
  CCTGTCGTATCCCAAACAAGCATCTCTTCTCACTGAATGCTGGAGAACGCGGCTGTTTCTGTCTTGATTT
  CTCTCACAATGGAAGGATATTAGCAGCAGCCTGTGCCAGCCGAGACGGATATCCGATTATATTATATGAA
  ATTCCTTCTGGACGTTTTATGAGAGAATTGTGTGGCCACCTCAATATCATTTATGATCTCGACTGGTCAA
  AAGACGATCGCTATCTCGTTACTTCATCCTCTGACGGCACTGCCAGAGTTTGGAAGAATGAAATCAATAG
  CACGAGCACGTTCAGAGTCTTACCTCACCCCTCCTTCGTCTACACGGCTAAATTCCACCCAGCCACACGG
  GAGCTGGTGGTTACAGGATGCTACGACTCTATGATAAGGATTTGGAAAATTGATGCAAGGGAGGATGCTG
  CCATATTAGTGCGCCAGTTGGATGTGCACAAGAGCTTTGTCAACTCCATCTGTTTTGACGATGAAGGTCA
  CCACATGTACTCAGGAGACTGCATCGGGGTCATCGTTGTCTGGGACACGTACGTCAAAGTGAATGATGTG
  CAACACTCCGTGCGCCACTGGACGATAAATAAGGAAATTAAGGAAACTGAATTCAGGGGAGTTCCAATAA
  GTTACCTGGAGGTTCATCCCAATGGAAAGCGTTTGCTAATCCACACCAAAGACAGTACTCTGAGGATTAT
  GGACCTGCGGATATTGGCAGCCAGGAAATTTGTGGGTGCAGCAAATTACCGTGAGAAGATCCATAGCACC
  TTGACGCCGTGCGGGACTCTGCTCTTTTCTGGGAGTGAGGATGGGATAGTATACGTTTGGAACCCAGAGA
  CAGGAGAACAAGTGGCAATGTACTCTGACCTGCCATTCAAGTCCACAATCCGAGACATCTCTTACCACCC
  CCTGGAGAATATGGTTGCCTTCTGTGCCTTTGGACAGAGCGAGCCGATTCTTCTTTATATTTATGATTTC
  CAGGTAGCCCAGCAGGAGGCTGAAATGTTGAAACGCTATAGTGGGACACTCCCGTTGCCTGGGATACATC
  AGAGTGAGGATGCTTTATGCACCTGCCCTAAGCTGCCCCAGCAGGGCTCTTTTCAGATTGATGAATTTGT
  CAACACTGAAAATAGTTCATCAAGAAAGATTCAACTTGTGAAACAGAGACTCGAAACTGTCACAGAGGTG
  ATACGATCCTGTGCTGCAAAAGTCAACAAAAATCTCTCAATGACTTCGCCACCACCAGGCCCTGCAAAGA
  AGCCCAGGGTAAAGCAGTCGTTCGTGCTGACCACTGATGAGATCATACATCAGTTTGGACTCCCCCAGAC
  TGCATTTATCAGCATAGAGAGAGGGCCTTTTGTGCGCCATGTTGATCCACCACCAATGGTAGTGGCTCTT
  TATGACTACACAGCCAGCCGATCAGATGAACTAACCATCCATCGTGGAGACATTATCCGAGTGTATTTCA
  AAGATAATGAAGACTGGTGGTACGGCAGCGTAAGAAAGGGGCAGGAAGGGTTCTTTCCAGCTAATCATGT
  GGCCAGTGAAACACTGTATCGAGACTCCCCTCCGAAGGTAAAGGAGCGCTCCCCTCCTTTAACTCCCAAG
  GAGAAAACTAAACCAGAAAAGCCTCTGGCTTCTCAAAAGCAGTCGCTCAGTAAGGGCAGACCCCTGGATC
  CCAGACTGGGCCCGCAGCCTGTGGGGCATTCTGAGAAGGGCAAAGATCAAAACGTGGAGGACCGAGGACA
  CAAAGTAGATATGGAGACAAAGAAAAGCGAGCCGGTGGTCCGCAAAGTCACCCTGATAGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR219438 representing NM_026203
  Red=Cloning site Green=Tags(s)
  
  MEPETPEKVDSAQEKVRGKTPTADDSDDSREKTGIEEKGELTDAYQLQVAEEMAKEIKKKIRKKLKEQLT
  YFPPDTLLHDDKLASEKRKKKKKKVPVPTKPESSPSDVCDSAVEGEQKKEGTPEDSQHMEGICSREQDVD
  ATVPENAKPKPKKTKKKTKAVSNDNEDTNGDGVHEITSRDSPVHPKCLLDDDLVMGVYIHRTDRLKSDFM
  ISHPMVKIHVVDEHTGQYVKKDDSERPVSSYYEKDNVDYILPIMTQPYDFKKLKSRLPEWEEQVIFNENF
  PYLLREFEECPKVILFFEILDFLSMDEIKNNSEVQNQECGFRKIAWAFLKLLGANGNANINSKLRLQLYY
  PPTKPRSQLNVVEVFEWWSKCPRNRYPSTLYVTVRGLKVPDCIKPSYRSMMALQEERGTPVYCERHRETS
  SVDTEPGLEDSKEEVKWKRLPGQACRIPNKHLFSLNAGERGCFCLDFSHNGRILAAACASRDGYPIILYE
  IPSGRFMRELCGHLNIIYDLDWSKDDRYLVTSSSDGTARVWKNEINSTSTFRVLPHPSFVYTAKFHPATR
  ELVVTGCYDSMIRIWKIDAREDAAILVRQLDVHKSFVNSICFDDEGHHMYSGDCIGVIVVWDTYVKVNDV
  QHSVRHWTINKEIKETEFRGVPISYLEVHPNGKRLLIHTKDSTLRIMDLRILAARKFVGAANYREKIHST
  LTPCGTLLFSGSEDGIVYVWNPETGEQVAMYSDLPFKSTIRDISYHPLENMVAFCAFGQSEPILLYIYDF
  QVAQQEAEMLKRYSGTLPLPGIHQSEDALCTCPKLPQQGSFQIDEFVNTENSSSRKIQLVKQRLETVTEV
  IRSCAAKVNKNLSMTSPPPGPAKKPRVKQSFVLTTDEIIHQFGLPQTAFISIERGPFVRHVDPPPMVVAL
  YDYTASRSDELTIHRGDIIRVYFKDNEDWWYGSVRKGQEGFFPANHVASETLYRDSPPKVKERSPPLTPK
  EKTKPEKPLASQKQSLSKGRPLDPRLGPQPVGHSEKGKDQNVEDRGHKVDMETKKSEPVVRKVTLIE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_026203
• ORF Size: 3141 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_026203.3, NP_080479.2
• RefSeq Size: 4885 bp
• RefSeq ORF: 3144 bp
• Locus ID: 52906
• Cytogenetics: 10 9.75 cM
• MW: 119.7 kDa