Fggy (NM_001113412) Mouse Tagged ORF Clone

CAT#: MR219072

Fggy (Myc-DDK-tagged) - Mouse FGGY carbohydrate kinase domain containing (Fggy), transcript variant 1

Product Images

Circular map for MR219072

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Fggy
• Synonyms: 2310009E04Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR219072 representing NM_001113412
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGTCTGGCAGAGACCAGGAACCAAGTCGCTACTATGTGGGCATCGATGTGGGAACAGGTAGTGTCC
  GTGCGGCTCTAGTGGACCAGAGAGGGCTCCTTTTGGCTTTCGCAGAGCAGCCAATTAAGAAGTGGGAGCC
  TCAATTCAACCACCACGAGCAGTCCTCAGAGGATATCTGGGCGGCATGCTGCCTTGTCACAAAGGAAGTT
  GTTCAAGGGATTGATGCACATCGAATCCGAGGACTTGGTTTTGATGCCACGTGTTCTCTGGTTGTCTTGG
  ATAAAGAGTTCCATCCTTTACCAGTAAACCATGAAGGGGACTCCTCTCGAAACGTTATCATGTGGCTGGA
  CCACCGGGCTGTCAGCCAGGTCCACAGGATTAATGAGACCAAGCACAGAGTCCTTCAGTATGTGGGTGGT
  GTGATGTCTGTGGAGATGCAGGCTCCAAAGCTCCTGTGGCTGAAGGAGAACTTGAGAGAGATTTGCTGGG
  ATAAGGCGGGACATTTCTTTGATCTCCCAGACTTCTTATCATGGAAGGCAACAGGTGTCACAGCACGGTC
  TCTCTGCTCCTTGGTGTGCAAATGGACATACTCAGCAGAGAAAGGTTGGGATGACAGCTTCTGGAAGATG
  ATTGGACTGGAAGATCTCATTGATGATAATTACAGCAAAATAGGAAACCTCGTGTTGCTTCCTGGAGCTG
  CTCTTGGAATTGGGCTCACACCAGAAGCAGCCAGAGAGCTGGGTCTTCCTTCAGGAATTGCAGTGGCAGC
  CTCACTCATTGATGCCCATGCAGGAGGACTAGGAGTGATTGGGGCAGATGTGAGAGGTCACGGGCTTACC
  TGTGAGGGACAGCCAGTGACGTCAAGGCTGGCTGTCATCTGTGGAACATCATCTTGTCACATGGGGATCA
  GCAAAGATCCAGTGTTTGTACCAGGTGTCTGGGGACCTTATTATTCAGCCATGGTCCCTGGGTTCTGGTT
  GAATGAAGGTGGTCAGAGTGTGACTGGAAAATTGATAGACCATATGGTGCAGGGCCATCCTGCCTTTCCT
  GAACTACAAGCCAAGGCCACAGCCAGATGCCAGAGTATATATGCATATTTGAATAGTCACCTGGATCTGA
  TTAAGAAGGCTCAGCCTGTGGGTTTCCTCACTGTTGATTTACATGTTTGGCCAGATTTCCATGGCAACCG
  GTCTCCCTTAGCAGATCTGACTCTGAAGGGCATGGTCACTGGATTGACGCTGTCTCAGGACCTTGATGAT
  CTTGCCATCCTCTACCTGGCCACTGTTCAAGCCATTGCTTTTGGGACTCGCTTCATCATTGAAACAATGG
  AGGCAGCAGGGCACTCTCTCAGTACCCTCTTCTTATGTGGAGGCCTAAGCAAGAATCCGCTTTTTGTGCA
  AATGCATGCTGACATTACTGGCATGCCTGTGGTCCTGTCACAAGAGGTGGAGTCTGTCCTCGTGGGAGCT
  GCTATTCTGGGTGCCTGTGCCTCAGGGGACTTCACTTCTGTGCAGGAGGCAATGGCAAGGATGAGCAAAG
  TGGGGAAAGTCGTGTTTCCGGAACATGCTGATAAAAAATATTATGACAAGAAATATCAAGTGTTTCTGAG
  AATGGTTGAGCACCAGAAGGAGTATTCAGCAATCATGAATGGAAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR219072 representing NM_001113412
  Red=Cloning site Green=Tags(s)
  
  MMSGRDQEPSRYYVGIDVGTGSVRAALVDQRGLLLAFAEQPIKKWEPQFNHHEQSSEDIWAACCLVTKEV
  VQGIDAHRIRGLGFDATCSLVVLDKEFHPLPVNHEGDSSRNVIMWLDHRAVSQVHRINETKHRVLQYVGG
  VMSVEMQAPKLLWLKENLREICWDKAGHFFDLPDFLSWKATGVTARSLCSLVCKWTYSAEKGWDDSFWKM
  IGLEDLIDDNYSKIGNLVLLPGAALGIGLTPEAARELGLPSGIAVAASLIDAHAGGLGVIGADVRGHGLT
  CEGQPVTSRLAVICGTSSCHMGISKDPVFVPGVWGPYYSAMVPGFWLNEGGQSVTGKLIDHMVQGHPAFP
  ELQAKATARCQSIYAYLNSHLDLIKKAQPVGFLTVDLHVWPDFHGNRSPLADLTLKGMVTGLTLSQDLDD
  LAILYLATVQAIAFGTRFIIETMEAAGHSLSTLFLCGGLSKNPLFVQMHADITGMPVVLSQEVESVLVGA
  AILGACASGDFTSVQEAMARMSKVGKVVFPEHADKKYYDKKYQVFLRMVEHQKEYSAIMNGS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001113412
• ORF Size: 1656 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001113412.1, NP_001106883.1
• RefSeq Size: 1904 bp
• RefSeq ORF: 1659 bp
• Locus ID: 75578
• UniProt ID: A2AJL3
• Cytogenetics: 4 C5
• MW: 60.8 kDa