Tank (NM_011529) Mouse Tagged ORF Clone

CAT#: MR219055

Tank (Myc-DDK-tagged) - Mouse TRAF family member-associated Nf-kappa B activator (Tank), transcript variant 3

Product Images

Circular map for MR219055

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tank
• Synonyms: C86182; E430026L09Rik; I-TRAF
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR219055 representing NM_011529
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTTTAAAGAGACATAGTCTGCGAAGGAACGCCTGTCACCTGGAGACGAGAGCTGGCATTCCTACCA
  TCCTTTATAGTGATGCTACAGGACAAAGAGGAATGGATAAAAACATTGGTGAGCAACTCAATAGAGCATA
  TGAAGCCTTCCGACAGGCATGCATGGATAGAGATTCAGCAGTAAGAGAGCTACAGCAAAAGCAGACTGAG
  AACTATGAACAAAGAATACGCGAGCAACAGGAACAGCTGTCATTTCAACAAAACCTAATTGACAGGCTGA
  AATCACAGCTACTTCTCGTGGATTCTAGTCGAGATAACAGTTATGGCTATGTACCTTTGCTTGAAGACAG
  TGACAGAAGGAAGAATAATTTGACCCTTGATGAACCACATGATAAAGTGAAACTAGGAACACTGAGAGAT
  AAGCAATCAAAGGTGAGACGACAAGAAGTTTCTTCTGGAAAAGAATCCGCCAAGGGTCTCAACATCCCTC
  TGCATCACGAAAGGGATAATATAGAGAAGACTTTCTGGGACCTTAAAGAAGAATTTCATAGGATTTGCTT
  GCTAGCAAAAGCACAGAAAGATCACTTAAGCAAACTTAATATACCAGATATTGCAACTGACACACAGTGT
  TCTGTGCCTATACAGTGTACTGATAAAACAGAGAAACAAGAAGCGCTGTTTAAGCCCCAGGCTAAAGATG
  ATATAAATAGAGGTATGTCGTGCGTCACAGCTGTCACACCAAGAGGACTGGGCCGGGATGAGGAAGATAC
  CTCTTTTGAATCACTTTCTAAATTCAATGTCAAGTTTCCGCCTATGGACAATGACTCTATTTTTCTACAT
  AGCACTCCAGAGGCCCCGAGCATCCTTGCTCCTGCCACACCTGAGACAGTGTGCCAGGACCGATTTAATA
  TGGAAGTCAGAGACAACCCAGGAAACTTTGTTAAAACAGAAGAAACTTTATTTGAAATTCAGGGAATTGA
  CCCCATAACTTCAGCTATACAAAACCTTAAAACAACTGACAAAACAAACCCCTCAAATCTTAGAGCGACG
  TGTTTGCCAGCTGGAGACCACAATGTGTTCTATGTAAATACGTTCCCACTTCAAGACCCGCCTGACGCAC
  CTTTTCCCTCACTGGATTCCCCAGGAAAGGCTGTCCGAGGACCACAGCAGCCCTTTTGGAAGCCTTTTCT
  TAACCAAGACACTGACTTAGTGGTACCAAGTGATTCAGACTCAGAGCTCCTTAAACCTCTAGTGTGTGAA
  TTCTGTCAAGAGCTTTTCCCACCATCCATTACATCCAGAGGGGATTTCCTCCGGCATCTTAATACACACT
  TTAATGGGGAGACT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR219055 representing NM_011529
  Red=Cloning site Green=Tags(s)
  
  MSLKRHSLRRNACHLETRAGIPTILYSDATGQRGMDKNIGEQLNRAYEAFRQACMDRDSAVRELQQKQTE
  NYEQRIREQQEQLSFQQNLIDRLKSQLLLVDSSRDNSYGYVPLLEDSDRRKNNLTLDEPHDKVKLGTLRD
  KQSKVRRQEVSSGKESAKGLNIPLHHERDNIEKTFWDLKEEFHRICLLAKAQKDHLSKLNIPDIATDTQC
  SVPIQCTDKTEKQEALFKPQAKDDINRGMSCVTAVTPRGLGRDEEDTSFESLSKFNVKFPPMDNDSIFLH
  STPEAPSILAPATPETVCQDRFNMEVRDNPGNFVKTEETLFEIQGIDPITSAIQNLKTTDKTNPSNLRAT
  CLPAGDHNVFYVNTFPLQDPPDAPFPSLDSPGKAVRGPQQPFWKPFLNQDTDLVVPSDSDSELLKPLVCE
  FCQELFPPSITSRGDFLRHLNTHFNGET
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011529
• ORF Size: 1344 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011529.2, NP_035659.1
• RefSeq Size: 2018 bp
• RefSeq ORF: 1347 bp
• Locus ID: 21353
• UniProt ID: P70347
• Cytogenetics: 2 C1.3
• MW: 51.4 kDa
• Gene Summary: Adapter protein involved in I-kappa-B-kinase (IKK) regulation which constitutively binds TBK1 and IKBKE playing a role in antiviral innate immunity. Acts as a regulator of TRAF function by maintaining them in a latent state. Blocks TRAF2 binding to LMP1 and inhibits LMP1-mediated NF-kappa-B activation. Negatively regulates NF-kappaB signaling and cell survival upon DNA damage. Plays a role as an adapter to assemble ZC3H12A, USP10 in a deubiquitination complex which plays a negative feedback response to attenuate NF-kappaB activation through the deubiquitination of IKBKG or TRAF6 in response to interleukin-1-beta (IL1B) stimulation or upon DNA damage. Promotes UBP10-induced deubiquitination of TRAF6 in response to DNA damage. May control negatively TRAF2-mediated NF-kappa-B activation signaled by CD40, TNFR1 and TNFR2. Essential for the efficient induction of IRF-dependent transcription following infection with Sendai virus.[UniProtKB/Swiss-Prot Function]