Tank (NM_011529) Mouse Untagged Clone

CAT#: MC215967

Tank (untagged) - Mouse TRAF family member-associated Nf-kappa B activator (Tank), transcript variant 3, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Tank
• Synonyms: C86182; E430026L09Rik; I-TRAF
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC215967 representing NM_011529
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTTTAAAGAGACATAGTCTGCGAAGGAACGCCTGTCACCTGGAGACGAGAGCTGGCATTCCTACCA
  TCCTTTATAGTGATGCTACAGGACAAAGAGGAATGGATAAAAACATTGGTGAGCAACTCAATAGAGCATA
  TGAAGCCTTCCGACAGGCATGCATGGATAGAGATTCAGCAGTAAGAGAGCTACAGCAAAAGCAGACTGAG
  AACTATGAACAAAGAATACGCGAGCAACAGGAACAGCTGTCATTTCAACAAAACCTAATTGACAGGCTGA
  AATCACAGCTACTTCTCGTGGATTCTAGTCGAGATAACAGTTATGGCTATGTACCTTTGCTTGAAGACAG
  TGACAGAAGGAAGAATAATTTGACCCTTGATGAACCACATGATAAAGTGAAACTAGGAACACTGAGAGAT
  AAGCAATCAAAGGTGAGACGACAAGAAGTTTCTTCTGGAAAAGAATCCGCCAAGGGTCTCAACATCCCTC
  TGCATCACGAAAGGGATAATATAGAGAAGACTTTCTGGGACCTTAAAGAAGAATTTCATAGGATTTGCTT
  GCTAGCAAAAGCACAGAAAGATCACTTAAGCAAACTTAATATACCAGATATTGCAACTGACACACAGTGT
  TCTGTGCCTATACAGTGTACTGATAAAACAGAGAAACAAGAAGCGCTGTTTAAGCCCCAGGCTAAAGATG
  ATATAAATAGAGGTATGTCGTGCGTCACAGCTGTCACACCAAGAGGACTGGGCCGGGATGAGGAAGATAC
  CTCTTTTGAATCACTTTCTAAATTCAATGTCAAGTTTCCGCCTATGGACAATGACTCTATTTTTCTACAT
  AGCACTCCAGAGGCCCCGAGCATCCTTGCTCCTGCCACACCTGAGACAGTGTGCCAGGACCGATTTAATA
  TGGAAGTCAGAGACAACCCAGGAAACTTTGTTAAAACAGAAGAAACTTTATTTGAAATTCAGGGAATTGA
  CCCCATAACTTCAGCTATACAAAACCTTAAAACAACTGACAAAACAAACCCCTCAAATCTTAGAGCGACG
  TGTTTGCCAGCTGGAGACCACAATGTGTTCTATGTAAATACGTTCCCACTTCAAGACCCGCCTGACGCAC
  CTTTTCCCTCACTGGATTCCCCAGGAAAGGCTGTCCGAGGACCACAGCAGCCCTTTTGGAAGCCTTTTCT
  TAACCAAGACACTGACTTAGTGGTACCAAGTGATTCAGACTCAGAGCTCCTTAAACCTCTAGTGTGTGAA
  TTCTGTCAAGAGCTTTTCCCACCATCCATTACATCCAGAGGGGATTTCCTCCGGCATCTTAATACACACT
  TTAATGGGGAGACTTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_011529
• Insert Size: 1347 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011529.2, NP_035659.1
• RefSeq Size: 2018 bp
• RefSeq ORF: 1347 bp
• Locus ID: 21353
• UniProt ID: P70347
• Cytogenetics: 2 C1.3
• Gene Summary: Adapter protein involved in I-kappa-B-kinase (IKK) regulation which constitutively binds TBK1 and IKBKE playing a role in antiviral innate immunity. Acts as a regulator of TRAF function by maintaining them in a latent state. Blocks TRAF2 binding to LMP1 and inhibits LMP1-mediated NF-kappa-B activation. Negatively regulates NF-kappaB signaling and cell survival upon DNA damage. Plays a role as an adapter to assemble ZC3H12A, USP10 in a deubiquitination complex which plays a negative feedback response to attenuate NF-kappaB activation through the deubiquitination of IKBKG or TRAF6 in response to interleukin-1-beta (IL1B) stimulation or upon DNA damage. Promotes UBP10-induced deubiquitination of TRAF6 in response to DNA damage. May control negatively TRAF2-mediated NF-kappa-B activation signaled by CD40, TNFR1 and TNFR2. Essential for the efficient induction of IRF-dependent transcription following infection with Sendai virus.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) represents use of an alternate promoter and 5' UTR and uses an upstream start codon, compared to variant 1. The resulting isoform (3) has a longer N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.