Ldb3 (NM_001039073) Mouse Tagged ORF Clone

CAT#: MR219038

Ldb3 (Myc-DDK-tagged) - Mouse LIM domain binding 3 (Ldb3), transcript variant 4

Product Images

Circular map for MR219038

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ldb3
• Synonyms: AW742271; PDLIM6; ZASP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR219038 representing NM_001039073
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTTACAGTGTGACTCTGACTGGGCCTGGGCCCTGGGGCTTCCGTCTGCAGGGAGGCAAGGACTTCA
  ACATGCCCCTCACTATCTCCCGGATCACTCCAGGCAGCAAGGCAGCCCAGTCCCAGCTCAGCCAAGGAGA
  CCTGGTGGTGGCCATTGATGGCGTCAACACAGACACCATGACCCACCTGGAGGCCCAGAACAAGATCAAG
  TCGGCCAGCTACAACCTGAGCCTCACTCTACAGAAGTCCAAGCGGCCTATTCCCATCTCCACGACAGCGC
  CTCCCATCCAGTCCCCGCTGCCAGTGATCCCCCACCAGAAGGTGGTAGCCAATTCTCCAGCCAATGCTGA
  CTACCAGGAACGCTTCAACCCCAGCGTCCTGAAGGACTCAGCCCTGTCCACCCACAAGCCCATTGAAGTG
  AAGGGCTTGGGAGGCAAGGCCACTATCATCCACGCTCAGTACAACACCCCGATCAGCATGTACTCACAGG
  ATGCTATCATGGACGCCATCGCGGGGCAGGCCCAGGCCCAGGGCAGTGACTTCAGTGGGGCGTCTCCGCT
  GGCGAGCCTCCCTGTGAAAGACTTGGCGGTGGACAGCGCCTCTCCTGTGTACCAGGCTGTGATCAAAACC
  CAAAGCAAGCCGGAAGATGAGGCTGATGAGTGGGCCCGCCGGTCCTCCAACCTGCAGTCTCGCTCCTTCC
  GCATCCTGGCCCAGATGACCGGGACAGAATACATGCAAGACCCTGATGAAGAGGCTCTGCGAAGGTCAAG
  GCCACAGGCCTCTGCTTACAGTCCTGCAGCCGCCGCTTCTCCAGCACCCTCTGCCCATACCAGCTACAGC
  GAGGGTCCAGCTGCCCCTGCACCCAAGCCTCGGGTCGTAACCACTGCCAGCATCCGGCCTTCTGTCTACC
  AGCCAGTGCCTGCATCTTCCTACAGCCCATCTCCAGGAGCCAATTACAGTCCAACTCCCTATACCCCTTC
  GCCAGCTCCTGCTTATACCCCCTCTCCTGCTCCCACCTATACCCCCTCACCTGCACCTACATACTCCCCT
  TCCCCTGCACCAGCTTATACCCCCTCACCTGCCCCAAACTATACCCCTACACCATCAGCTGCCTACAGTG
  GGGGCCCTTCAGAATCTGCCAGCCGTCCACCCTGGGTGACCGATGACAGCTTCTCTCAGAAGTTTGCTCC
  TGGAAAGAGCACTACCACTGTCAGCAAGCAGACCCTGCCCCGGGGGGCTCCAGCCTACAACCCAACAGGT
  CCACAAGTGACACCTCTTGCCAGGGGCACCTTCCAGAGAGCTGAGCGCTTCCCAGCTAGCAGCCGGACTC
  CCCTCTGTGGGCACTGTAACAATGTCATCAGGGGCCCTTTTCTGGTGGCCATGGGCCGCTCTTGGCACCC
  AGAAGAGTTCAATTGTGCCTACTGCAAGACCTCCCTAGCAGATGTGTGCTTTGTGGAGGAGCAGAACAAC
  GTTTACTGCGAGCGGTGTTACGAGCAGTTCTTTGCCCCGATCTGTGCCAAATGTAACACCAAAATCATGG
  GGGAAGTGATGCACGCTCTGCGACAGACATGGCACACCACCTGCTTTGTCTGTGCAGCCTGCAAGAAGCC
  CTTTGGGAACAGCCTCTTCCACATGGAGGATGGAGAGCCATACTGTGAGAAAGACTACATCAACCTGTTC
  AGCACCAAGTGCCATGGATGCGATTTCCCTGTGGAAGCTGGTGACAAGTTTATCGAGGCCCTGGGGCACA
  CCTGGCATGACACCTGCTTCATTTGTGCGGTCTGCCACGTGAATCTGGAGGGTCAGCCATTCTACTCTAA
  GAAAGACAAACCACTGTGCAAGAAGCACGCACATGCCATCAACGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR219038 representing NM_001039073
  Red=Cloning site Green=Tags(s)
  
  MSYSVTLTGPGPWGFRLQGGKDFNMPLTISRITPGSKAAQSQLSQGDLVVAIDGVNTDTMTHLEAQNKIK
  SASYNLSLTLQKSKRPIPISTTAPPIQSPLPVIPHQKVVANSPANADYQERFNPSVLKDSALSTHKPIEV
  KGLGGKATIIHAQYNTPISMYSQDAIMDAIAGQAQAQGSDFSGASPLASLPVKDLAVDSASPVYQAVIKT
  QSKPEDEADEWARRSSNLQSRSFRILAQMTGTEYMQDPDEEALRRSRPQASAYSPAAAASPAPSAHTSYS
  EGPAAPAPKPRVVTTASIRPSVYQPVPASSYSPSPGANYSPTPYTPSPAPAYTPSPAPTYTPSPAPTYSP
  SPAPAYTPSPAPNYTPTPSAAYSGGPSESASRPPWVTDDSFSQKFAPGKSTTTVSKQTLPRGAPAYNPTG
  PQVTPLARGTFQRAERFPASSRTPLCGHCNNVIRGPFLVAMGRSWHPEEFNCAYCKTSLADVCFVEEQNN
  VYCERCYEQFFAPICAKCNTKIMGEVMHALRQTWHTTCFVCAACKKPFGNSLFHMEDGEPYCEKDYINLF
  STKCHGCDFPVEAGDKFIEALGHTWHDTCFICAVCHVNLEGQPFYSKKDKPLCKKHAHAINV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001039073
• ORF Size: 1866 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001039073.2, NP_001034162.1
• RefSeq Size: 4737 bp
• RefSeq ORF: 1869 bp
• Locus ID: 24131
• UniProt ID: Q9JKS4
• Cytogenetics: 14 B
• MW: 67.5 kDa
• Gene Summary: May function as an adapter in striated muscle to couple protein kinase C-mediated signaling via its LIM domains to the cytoskeleton.[UniProtKB/Swiss-Prot Function]