Adad1 (NM_009350) Mouse Tagged ORF Clone

CAT#: MR219030

Adad1 (Myc-DDK-tagged) - Mouse adenosine deaminase domain containing 1 (testis specific) (Adad1)

Product Images

Circular map for MR219030

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Adad1
• Synonyms: Tenr
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR219030 representing NM_009350
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGACCGCGGGCGGCAGCCGACGAGCCCCGGTCCCGGGCCCTCGGCTCGGCCTGCCTCTGGCAGCTC
  ACCTGCCGGCGAGCCTGGGCGGGGAGGGCGCGAAGGACAGCGTCGGGGGAGAGAAAACTTCTGGAAACAA
  TGACTGGTTTCAGAGTTCCAGGGTTCCCAGCTTTGCTCAGATGCTGAAAAAGAATTTGCCAGTTCAGCCA
  TCAGCTCAGACAGTAACTTTGCCCACTGGATATTCCTCTGAAAGTTGTAGTCTGTCGAATATGGCATCCA
  AGGTTACGCAAGTGACAGGTAATTTTCCAGAGCCATTGCTTTCCAAAGGTCTTTCATCTATTTCAAATCC
  TGTCCTTCCTCCAAAAAAACTACCTAAAGAGTTTATAATGAAATACAAACGTGGAGAGATCAATCCCGTG
  TCGGCCTTGCACCAGTTTGCACAAATGCAGCGTGTTCAGCTTGACCTTAAGGAAACTGTAACAACAGGAA
  ATGTTATGGGACCATATTTTGCTTTTTGTGCTGTGGTGGATGGTATTCAGTACAAGACAGGACTGGGACA
  GAATAAAAAGGAATCAAGATCCAATGCAGCAAAGTTAGCTCTTGACGAGCTTCTACAACTGGATGAGCCT
  GAACCGAGAGTTTTAGAACCAGCAGGACCACCTCCTATCCCTGCAGAACCTGTTGTTACACCTGAAGCAG
  CATATGTTTCAAAAGTGCAATATGAAGGGAGACAAGTCCAGTATGCCAAGATTTCACAGCTTGTTAAAGA
  AACATTTGGTCAACTAATTTCTAATCACTCACAATATCTGAAATGTAGCAGTTCATTGGCTGCTTTCATT
  ATTGAAAGAGCTGGACATCATGAAGTTGTGGCTATAGGCACAGGCGAATACAACTACAGCCAGTGCATCA
  AGCCAAATGGAAGGGTGCTGCACGATACTCATGCAGTTGTTACAGCAAGAAGGTCTCTCCTTAGGTACTT
  TTATAGACAGCTTCTGCTCTTCTATAGCAAAAATCCTGCTATGATGGAAAAGTCAATCTTTTGTACAGAA
  CCAGCTTCTAATCTACTTACTCTGAAACAAAACATCAACCTTTACCTCTATATGAACCAGTTGCCGAAAG
  GATCAGCCCAGATTAAGTCACAGTTACGCCTTAATCCACATTCTATTTCTGCGTTTGAAGCCAATGAAGA
  GCTGAGTCTCCATGTGGCTGTTGAAGGCAAAATTTATCTTACTGTTTACTGTTCTGCAGACGGGGTTAAT
  AGAGTAAACAGTATGTCCTCAAGTGACAAGCTGACAAGATGGGAGGTGCTTGGTGTACAGGGAGCCTTGC
  TGAGTCACTTTATACAGCCAGTTTATATCAGCAGTATACTTGTTGGTGATGGGAATTGCAATGACACAAG
  AGGCTTAGAAATCGCAATAAACCAGCGTGTTGATGACGCACTCACATCAAAACTGCCCATGTTTTACTTG
  GTCAACAGACCTCATATTAGCTTAGTGCCCACCGCATATCCTCTGCAGATAAACTTGGACCATAAATCAC
  TCAGTTTGAATTGGGCACAAGGGGACAATTCTTTGGAGATTGTGGATGGCCTAAATGGGAAGATTACTGA
  AAGTTCTCCATTTAAAAGTGGTTTATCAATGGCAAGTCGGCTTTGTAAGGCTGCAATGTTAAGTCGATTT
  AATCTGCTTGCCAAGGAAGCTAAAACAGATGATTTACTTGAAGCTCGGACATACCACGCGGCTAAGTGTA
  TGTCTGGACCCTATCAAGAAGCAAAGGCGTTACTGAAGGCCTACTTGCAGCAGCATGGCTATGGCTCCTG
  GATTGTGAAATCTCCCTGTATAGAGCAATTTAGTATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR219030 representing NM_009350
  Red=Cloning site Green=Tags(s)
  
  MATAGGSRRAPVPGPRLGLPLAAHLPASLGGEGAKDSVGGEKTSGNNDWFQSSRVPSFAQMLKKNLPVQP
  SAQTVTLPTGYSSESCSLSNMASKVTQVTGNFPEPLLSKGLSSISNPVLPPKKLPKEFIMKYKRGEINPV
  SALHQFAQMQRVQLDLKETVTTGNVMGPYFAFCAVVDGIQYKTGLGQNKKESRSNAAKLALDELLQLDEP
  EPRVLEPAGPPPIPAEPVVTPEAAYVSKVQYEGRQVQYAKISQLVKETFGQLISNHSQYLKCSSSLAAFI
  IERAGHHEVVAIGTGEYNYSQCIKPNGRVLHDTHAVVTARRSLLRYFYRQLLLFYSKNPAMMEKSIFCTE
  PASNLLTLKQNINLYLYMNQLPKGSAQIKSQLRLNPHSISAFEANEELSLHVAVEGKIYLTVYCSADGVN
  RVNSMSSSDKLTRWEVLGVQGALLSHFIQPVYISSILVGDGNCNDTRGLEIAINQRVDDALTSKLPMFYL
  VNRPHISLVPTAYPLQINLDHKSLSLNWAQGDNSLEIVDGLNGKITESSPFKSGLSMASRLCKAAMLSRF
  NLLAKEAKTDDLLEARTYHAAKCMSGPYQEAKALLKAYLQQHGYGSWIVKSPCIEQFSM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009350
• ORF Size: 1857 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009350.3, NP_033376.2
• RefSeq Size: 1972 bp
• RefSeq ORF: 1860 bp
• Locus ID: 21744
• UniProt ID: Q5SUE7
• Cytogenetics: 3 B
• MW: 67.8 kDa
• Gene Summary: Plays a role in spermatogenesis. Binds to RNA but not to DNA.[UniProtKB/Swiss-Prot Function]