Ano8 (NM_001164679) Mouse Tagged ORF Clone

CAT#: MR219012

Ano8 (Myc-DDK-tagged) - Mouse anoctamin 8 (Ano8)

Product Images

Circular map for MR219012

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ano8
• Synonyms: BC053460; Tmem16h
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR219012 representing NM_001164679
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGAGGCGGCTTCGGGCGCCGGGGACGTGACCCTGGAGGGGGAGCGGGGCAAGAGGCCCCCTCCCG
  AGGGCGAGCCTGCGGCGCCTGCGTCTGGAGTACTGGATAAACTATTTGGGAAGAGGCTCCTGCAGGCTGG
  CCGCTACCTGGTGTCCCACAAGGCATGGATGAAGACAGTGCCCACTGAGGACTGCGACGTGCTCATGACC
  TTCCCAGACACCACTGATGACCACACGCTGCTGTGGCTGCTGAATCACATCCGGGTGGGCATTCCCGAGC
  TGATCGTGCAAGTTCGCCACCACCGCCACACGCGTGCCTATGCCTTCTTTGTCACCGCCACATACGAGAG
  CCTGCTCCGTGGGGCCGATGAGCTGGGTCTGCGCAAGGCCGTGAAGGCCGAATTTGGAGGGGGCACCCGC
  AGCTTCTCCTGCGAAGAGGACTTCATCTACGAGAATGTGGAGAGCGAGCTACGCTTCTTCACATCGCAGG
  AGCGGCAGAGCATCATTCGCTTCTGGTTGCAGAACCTTCGAGCCAAGCAGGGGGAGGCTCTGCACAACGT
  GCGCTTCTTGGAGGACCAGCCAATCATTCCGGAGCTGGCTGCACGAGGCATCATACAGCAGGTGTTTCCA
  GTTCACGAGCAGCGTATTCTGAATCGTCTCATGAAGTCCTGGGTGCAGGCCGTGTGTGAAAACCAGCCTT
  TAGATGATATCTGTGACTATTTTGGGGTGAAGATTGCCATGTATTTTGCCTGGCTGGGCTTCTACACATC
  GGCAATGGTTTATCCTGCAGTTTTTGGCTCTGTTCTGTATACATTCACTGAGGCTGATCAGACAAGCCGG
  GATGTTTCCTGTGTGGTCTTCGCTCTCTTCAATGTGATCTGGTCAACACTGTTCTTGGAGGAGTGGAAAC
  GGAGGGGGGCAGAGCTAGCCTACAAGTGGGGTACACTAGACTCACCTGGGGAGGCTGTGGAGGAGCCACG
  TCCCCAGTTCAGGGGCATCCGGCGCATCAGCCCCATCACCCGGGCTGAGGAGTTCTACTACCCACCCTGG
  AAGCGATTGCTTTTCCAACTACTTGTCAGCCTGCCTCTGTGCCTGGCCTGTCTTATCTGTGTCTTTATAC
  TGATGCTTGGCTGCTTCCAGCTGCAGGAGCTAGTCTTGAGTGTGAAGGGTCTGCCCCGCCTGGTTCGCTT
  CTTGCCCAAGGTTATGCTAGCCCTGCTGGTCAGCGTGAGTGCTGAGGGCTACAAGAAGTTAGCTGTGTGG
  CTAAATGACATGGAGAATTACCGGCTAGAGAGCACCTACGAGAGGCACCTTATCATCAAAGTCGTCTTGT
  TCCAGTTTGTCAACTCGTACTTGAGTCTGTTCTACATCGGCTTCTACCTCAAAGACATGGACCGCCTAAA
  AGAGATGCTGGCCACCCTACTCATCACCCGGCAGCTGCTGCAGAACGTGCGCGAGGTGCTGCAGCCGCAC
  CTGTACCGCAGGCTGGGCAGCGGGGAGCTGGGCTTGCGAACCATCCTGGAGCTGGCCCGGGCCCTGCTAG
  GCCTGCTGAACCCCTTGCGCCCGGATCCCCGACGGCACTTGGAGGCCCAGGCTGATGAGGGCGGAGCTGG
  GAGCCGCAGGTGCCTGGGCGGTGGCTGCGGGGCGCCAGAGGAGGAGAATGAGGAGGAGGAGGAGGCAGCT
  GTGGAGCGGAGGCCAGCAGGGGAAGGAGGGGAGGTTCCAGAAGGGCCTCGGGGTGGCAAGGAGGAGGACG
  AGGAGGAGGACGACGACGAAGACGAGGACGAGGAATACGAGGGCGAGGAAGGAAGCCTCCTAGACTGCGG
  GCTGCGCCTCAAGAAGGTCAGCTTTGCTGAGCGGGGCGCAGGGCGGCGCAGGCCAGGCCCCAGCCCAGAC
  GGCCTCTTGGAGGAGGGGAGCCCTACCATGGTGGAGAAGGGGCTGGAGCCTGGTGTGTTTACTCTGGCCG
  AAGAAGACGATGAGCCTGAGGGGCCTCCAGGCAGTCCCGGGCCAGAGCCCCAGACTGTCTTGCTTCGTCG
  GGCGAGGGGCGAGGGCCGTGACCAAGGTCCTGATGGAGACCGAGATACGGAGACTGGCTCTGGCGACGCG
  GCTGGAAGACAGAAAAGACATAACAGGTCCTCGTGGATCGACCCGCCAGAGGAGGAGCATTCACCGCAGC
  TCACGCAGGCAGAGCTAGAGAGCTGCATGAAGAAGTATGAGGACACCTTCCAGGACTACCAGGAGATGTT
  CGTGCAGTTTGGCTATGTAGTGCTCTTCTCCTCTGCCTTCCCACTGGCCGCCCTGTGCGCCCTCGTCAAC
  AACCTGATTGAGATCCGAAGTGATGCCTTCAAGCTCTGCACTGGCCTGCAGAGGCCCTTTGGCCGGCGTG
  TGGAGAGCATTGGCCAGTGGCAGAAGGTCATGGAGGCGATGGGTGTGCTGGCCATCGTGGTAAACTGCTA
  CCTCATTGGCCAGTGTGGCCAGCTGCAGCGCCTGTTCCCCTGGCTGAGCCCAGAGGCGGCCATTGTATCT
  GTGGTGGTGCTAGAGCACTTGGCTCTTCTAGTCAAGTACCTCATCCATGTAGCCATCCCTGACATCCCCG
  GCTGGGTAGCTGAGGAGATGGCCAAACTGGAGTACCAGCGACGGGAAGCTTTCAAGCGGCACGAGCGGCA
  GGCGCAGCAGCGCTTTCAGCAGCAGCAGCGGCGGCGGCGCGAGGAGGAGGAGCGGCAGCGGCACGCGGAG
  CAGCAGGCGCGGCGGGAGCGCGACACGGGAGGCCGCGAGGAGGCGCGCGCGGAGGCGCCGGGTCCTGACC
  CCGTGGCGGAGCGGGGCGCTGCCAAGGCCAAGGGCAGCGAGCGGCCCCGACGGCCCGGAGCGCTGCTGCC
  ACCCGGACCTGTGCTGCGGCTGAAGCAGATCATCCCGTTGCAGACTCGGCCGCCCGCGCCCACCGGGTGC
  GCGCCCCCGCCGCGCTCGCCCGCGGACACGCGCCTGCCCGCCTTCCTCAGCCTGCGCTTCCTCAAGGCTC
  CGGAGCGAGGCCCGTCCCCGCCGCGGCCCGGGAAGCTGTTCGCATTCTCGGCGCGCGAGCCCTCAGCCAA
  CGGGGCCCCCGGGGGCGGCGCGCGCGCTCACAGGAGCGCCGGGGACGAGCCCGCGGCCGCCGAGCCCGAA
  CCGCGGCCGGAGGACGCAGGTCACAGGCCT
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR219012 representing NM_001164679
  Red=Cloning site Green=Tags(s)
  
  MAEAASGAGDVTLEGERGKRPPPEGEPAAPASGVLDKLFGKRLLQAGRYLVSHKAWMKTVPTEDCDVLMT
  FPDTTDDHTLLWLLNHIRVGIPELIVQVRHHRHTRAYAFFVTATYESLLRGADELGLRKAVKAEFGGGTR
  SFSCEEDFIYENVESELRFFTSQERQSIIRFWLQNLRAKQGEALHNVRFLEDQPIIPELAARGIIQQVFP
  VHEQRILNRLMKSWVQAVCENQPLDDICDYFGVKIAMYFAWLGFYTSAMVYPAVFGSVLYTFTEADQTSR
  DVSCVVFALFNVIWSTLFLEEWKRRGAELAYKWGTLDSPGEAVEEPRPQFRGIRRISPITRAEEFYYPPW
  KRLLFQLLVSLPLCLACLICVFILMLGCFQLQELVLSVKGLPRLVRFLPKVMLALLVSVSAEGYKKLAVW
  LNDMENYRLESTYERHLIIKVVLFQFVNSYLSLFYIGFYLKDMDRLKEMLATLLITRQLLQNVREVLQPH
  LYRRLGSGELGLRTILELARALLGLLNPLRPDPRRHLEAQADEGGAGSRRCLGGGCGAPEEENEEEEEAA
  VERRPAGEGGEVPEGPRGGKEEDEEEDDDEDEDEEYEGEEGSLLDCGLRLKKVSFAERGAGRRRPGPSPD
  GLLEEGSPTMVEKGLEPGVFTLAEEDDEPEGPPGSPGPEPQTVLLRRARGEGRDQGPDGDRDTETGSGDA
  AGRQKRHNRSSWIDPPEEEHSPQLTQAELESCMKKYEDTFQDYQEMFVQFGYVVLFSSAFPLAALCALVN
  NLIEIRSDAFKLCTGLQRPFGRRVESIGQWQKVMEAMGVLAIVVNCYLIGQCGQLQRLFPWLSPEAAIVS
  VVVLEHLALLVKYLIHVAIPDIPGWVAEEMAKLEYQRREAFKRHERQAQQRFQQQQRRRREEEERQRHAE
  QQARRERDTGGREEARAEAPGPDPVAERGAAKAKGSERPRRPGALLPPGPVLRLKQIIPLQTRPPAPTGC
  APPPRSPADTRLPAFLSLRFLKAPERGPSPPRPGKLFAFSAREPSANGAPGGGARAHRSAGDEPAAAEPE
  PRPEDAGHRP
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001164679
• ORF Size: 3180 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001164679.1, NP_001158151.1
• RefSeq Size: 3773 bp
• RefSeq ORF: 3183 bp
• Locus ID: 382014
• UniProt ID: Q6PB70
• Cytogenetics: 8 B3.3
• MW: 119.6 kDa
• Gene Summary: Does not exhibit calcium-activated chloride channel (CaCC) activity.[UniProtKB/Swiss-Prot Function]