Alpk2 (NM_001037294) Mouse Tagged ORF Clone

CAT#: MR218950

Alpk2 (Myc-DDK-tagged) - Mouse alpha-kinase 2 (Alpk2)

Product Images

Circular map for MR218950

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Alpk2
• Synonyms: Gm549; Hak
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR218950 representing NM_001037294
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACAGATCCAGGCTGCCCCGAGAGGCGCACGCTATGTTTCTTATCAACGTTACTTTCCCAGAAGGTTC
  CGGAGAAGTCTGACGTCGTGCTTCGATGCATGATAGCTGGTCAGCCCAAGCCAGAGGTGACTTGGTATAA
  GAATGGTCAAGCCATAGACTTGGGAGGCACTGTTTCCAGTTATGAGTTCTTTGAGAACCAGTATATTCAT
  TTGTTGCATCTCTCGTGCTGTACCCAGAGCGACGCCGCTGTTTACCAGGTCTCGGCAAGAAACTGCGTGG
  GGATGATCTGCTGTTCAGCATCCCTCGAGGTCCAGTGTCTGCAGGACCCACAGGTCTCTCCTGATCCTGG
  AGGCGGCAGGGACGCGGCTGGCGAGTGTAAAACAGAGATACGTGAAGAAGACAGCATAAACCACACGGAT
  GAGAAATGGAATCCTTGTAAGAAAGGAGAAAGTACAGCTGATTCCTTCCTCGACAAATTCAACCATTTGA
  GTTCACCCCAGATAGTGGCTAGGGGTGACTCTGGTGCTTCCAATTCTGAGAATCCCCAATATATTAAAGA
  GACGAGACAAAGGATGGGGCAGTACAATTCAAATAACATGCAAGAAAATTCTTTTAATTCAAATAACACT
  GCTGAAAAACAAGATGTATCCCAGCTCTGGACAGTGAATGCCACTGTGCCAGGGTTAGTTAGTGATGGCC
  TAGGATACGAAGAATCTAATGAGAGTGTCAGCCCTAGTCATCAAACCCCCAAAGTACAGAAATACATCAG
  TTTCAGTCTTCCATTGCCGGAGACAACCCTGGGTCCTTACCCAGAAGACAGCAACTCCATCAACATGCAG
  CCGGGACCCCAGGTTTCCAGCGAAGACTCTGACAGTGACTATGAACTTTGCCCAGAGATAACCCTAACCT
  ACACGGAGGAGTTTTCGGATGATGACCTGGAGTATCTGGAATGTTCCGATGTTATGACGGATTACTCTAA
  TGCGGTCTGGCAAAGGAGCCTGCAAGGGACTGACCGAGTTTTTTTATTAGAAAGCGATGACGAAGAGATG
  GAATTCAATGAGTGTGGTCTGGGTGGGTGTGAGCATTTCTTCACTGAAATGGGTTGTGGGCCTCAGGTGT
  CGGGTGGCATGTGGTCTATGAATGTTGCCACTGGCTTCTGTAGTTATCACTCACAACCTCAGGAAGTGAG
  GGTTAGGAGCAGCGGAACCTCAGGGCATAGCCCGTTACCCCTGCATTCAGAAATGACTCTTACCCTGGGA
  CCTCACCAGGATGAAACAGCTAAGATGACAGAACCGGGGAGGGCTCCACTCCCTACTGCTCCCGAGGCTG
  TTGAAAATGATTGTTCAGGAATTCGGGGAGAAACCAGAGACAACCCTGAAGCAGGAGAGGAATTCTCCGG
  TGACAATCTACAGACTATGGATAAGGTAGAGACAGAGGCATCAGTGAAGCCCTTGTCTGGGGGCTCAGAT
  AAGACAGAGGTGAAGCAGGGTTTGGAAAGCCTGGCTCGGGAACGCACAGACGAAAAGTACCCAGGCAGCA
  AGAAGGCAGCCCTGAGACCCACCAGGGCGAGGCGGCCCGGAATGAAGGCAAACACCAAGAAGCAGCTGTT
  GAGAGACAGTGCCCCCAAAGGCACTCTAGATCTCCTCCCCAAAGAACCGACTAGGCAACCCTTACCCGGG
  AGCTATGGACAAGAGCCCACACACACGGAAGCAGGAGCTCCTGGCTGGGATTCCCACTTCCACGCAGAAG
  TGTGTATTCCACTTCCTGCAGAGCAAGACTCCAAAATCCTTCGACCACCAGCAGACCCACTTTCCAAGGA
  AGAGGACAGCAGCTTCGAGGGAGGTGGGGCACTGCTTAATAAATTATTTGAAGCAAGCCAGATTCCAGAC
  CGGACTGACCATCTTCAGATGCAAATTCAGGAAACCATTGGGGAGAGCAGTTCTCTCGACCAGATGTTGG
  CCTTTTCAGTGCCTGCTGAGGAGTCATCTACATTTGCTGGGGCCACAACACATTCTGTCTCCAACTTGAG
  TGAGATCAACAGGGAAAATCTGTCATTGGCCCAATACCCAGGACTGGAAAGCTGTCCTCAAAGCCTCCAG
  CAGGAAGGCAGACCAAACAGAGACAGAGACTTGCCTGGTGCTCTCTGGGCAGAATCAGCCTGTGAACTGA
  GTCTCCTAGAAGACAATGAGGAAGAAGAGTCGCAGCCTCCAGCCTCAGTGGCTCTCCCTCAGGGTGATGG
  TGTCCCCTGCAGGGAGCCAGAGGGTCTCTCTGATTCTTTCCCCCAGCCCACTGCTCCCTCCCTCCCCCTG
  GAAAATGTGGGCAGTGGGTCAAGGGTCAGAGAAGCTGCAGGTGGGGTGGGGTGTTTTGAAGCCGGTGACC
  AAGAAACATGTTATGCTACCATGGATCTCCTTGTTGGAGCACCAGTTGATAAATATTTGCCTCAAGAAAT
  TTGCCCCGAGGACTTGGAGCTGACAGAAGGTCAAAGCGAAGTGTGTGATTTATGTTCTCCTGACAAGATA
  CTGGCTGTTCTACAGACACAAGGTTCTGAGCCTCCACGGTCCACAGACAAGCGCAGCCAGGATGGCAAGT
  CAGCCGAGGGCCTTCTTTTTAACAGTACCTTCACCTGGGACACGGCAAAGGAGGCCAGTGAAGATGCTGT
  GGGAGAGACAGCAGCTGATGTGGAGAATCCTCCCTCCACCTTCTCTTCTACGCTACCCTACAGTGAAAGA
  GGGTTTGGGGAGACACAACCCCTTTGTTCTGAGACTATCTCCTTTGTAAAGGATAGTGAAGGGAGCTACA
  GAAGTTCCAGTCTCAGCATCCCAGCTGCCATAGACACACTTGCCAGCTACAGTTCTGACAGGGAGTGCTC
  AAAAGAGCAGTCAGCCGAATCAACTGCTAATGTCGACTGTCATCAGGTGACCAGGGAGATGGAGGGCATA
  TCAACTAATGCCGCTGAGGTCCACGAAATCAAATGCCACTCCGTTTCTGTCCCCCAGGACAATGACTTTG
  ATGTTGGTGCTGACCAGGTCTCGTGTGAGGCACGAGATGAAGATAATTCCCAATCTCTTCCAGACGACGA
  CTCACAGTCAGGTCGTTCATTAAGTAGCTCCACAGGTGAAGCAACCGGGGAGACTCTGGTGCCAGCACCC
  AGCAGTGCAGGAGATCATGGCCACTTCTCCATGCCCGAGGGACAGGGTTTGTGTAGCAGGGCTCTTCAGA
  TGGATAACCAGCCTGTGTGTCAGAGCCAGGCTATGGAGGGAGCCCACAGCAGAGGCCTTGAGGAGCACTT
  CCAAGAAAAGGGAAGTGGAATGAAGCATGGCATCCGGCCACAGAGCACATCCCACCAGGTTTCTCTTTCT
  GCAAATGACTTCCAAGAAATTTTGCCCTCCATACCCACCATGCAACAGGAGACCAATGTGGAACCCTTGG
  AGCACTCCCTAGCAGATTCCAGGGAAGAAATTGAGTGTAGCTCAGACCCGAGGACCAGTGACTTGGTGGT
  GGCTGAGAAGACTGTGGGAGAAGACAGTCATTTGGTAGTCAGTGTCCCAGCTCTCCCTGACATCCTCCTT
  GGAGAGAAAGATGACGTTGGGCTAGGAAGTTGGGCTGTGGGCGGCAAAGTGAAGATCATAACTCTAGAAG
  CTCCCGTCTTTGAAATCTGGCCACCAGAACTAGTGAGGCACCCTGGGTACAAGGAGGCAGAAGCTGGTCT
  CACCATGCCTGGTAGGAGCTGGGCTCTGTCTGACATCCTCAGAGCAGGTGCCACCAGATCTGAGCCAGGT
  GCCTTGGGAGGAGCAGCATGGGTTCCCAGCCCCCAGGCTGATGCTCTCATGGCCCTTGGAGCGAACAGGG
  ACACCTGGCTAGGTGCTGCACCAGACAGACAAGCAAACTGCAATTGTCTGTCTTCCCAGTGTCTGAGTCA
  ACCCCGATTCCTGGAGTCATCTGTAGACCCTGTTGAGGACAAGGAGTTAGAGGTCACGGACTCTCCATCA
  GAGGTTTCCAAAACTGGAGAGATGGAAATGCCTGAGACTCTGAATGAGGAACAGGAGGAAACCCAGCAGA
  TACTGCGTCACCCCGCCGTTGTTAACCAGTCGGTGAACTTCCCTAGGATCCTGGAATCCTCTGTGGACCC
  CATTGACGACAGGGGTGAGCTGGAGGGTGTCTGGCCCGAGAAGCCAGAGCCCTCTGACTCCAGCGTAGAA
  GGAAACGAATTCATTGTTGGAAACACGTGTCAGAGGGTAGACATCCAACCTGCTAGCCTACAGCTCCCAC
  ATCCCCAGGACAGCGGGGAAATCATTCCATATGAACACACAACCAACCAAAATCGCGTAGATGGAGAGAG
  AGCAGAAGCCAAAACCAGTCTGCCGGATAAAGCCAAAGCGGAAGCAGAAGCTGTTGTTTGGCAGGCCCAG
  GGGCCTGGTGAAGAGGGACAAGGAATTCCAAGTGTATGCAGCATGAGCCAAACACAAGATGGTGGTGACA
  GAAGCCTAGGAGAAGCTGGGCAAAGGGGAACGGATGAGACCGAGGTCATTTCCCCCCTGTCTCCTCTTTC
  TAGCTGTCTCACAGGAGTGACACATACATGTGTCAAGGCTGAAACCAACAACTCCACAGGCCACATTTAT
  GGCGGATCTGAGCCCAGAACCCGTCAAAGTGTAATTCCTATGAAGACAGAAAAGGGAACTATCGAGAGCA
  AGTGTGGGAACCATGTGCGCTCTTCAGATGATCTCACAAACACACCTTGTACTTCATCTCCCAAAGGAAA
  TGTCACACGCTTGTCAATAAGCCATGGCCTGGAGGAACTGAAATCAGAGAAGCTGCAGATTGCGGAAACC
  AAACCCCTAAACTCATCTGACTCCCCAACAATGACCTTAGCTCTCATTTCAGGAGAATGTGAGTCAGAGA
  AAGACCCCAAAAGCTTGTTACGTAGGGACCCATGTCCAAAGGGCTCCACCCTGGATAGCGGGAAGAAGTC
  CAGAGACCAACAGCAGAAGCCTGTGGCAGCCCAGGTCAGCAAGGCACCTGGGGACCAATCAGCAATGGCT
  GGGTCAGAGGAGGGCAAGAAGAAGCAAGAGGCTTCGGGGAGTGGACACTTGACTGCAGGGATAAAGAAGA
  AAATTCTATCCAGGGTCGCAGCCCTGAGACTGAGGCTGGAGGAAAAGGAAAATTCGAGGAAGAACTCCAT
  CGTGAAGAAGACACCTAAGTTTGAAAGGTCCTTATCCCGCACTGATGAGAAAAGAGACCCCAAAAGGGCC
  CCTTGCAAAGCTGAAGGGAAAGCTCCAGTATTGCTGAAGAGGATCCAGGCCGAGATGGCTCCCGAGCACT
  CCGGAAATATAAAGTTGAGCTGCCAGTTTTCAGAAATCCATGAAGACTCTACCGTCTGCTGGACAAAAGA
  TTCCAAGTCGATAGCCCAGGCCAAGAAAAGCGCAGGGGACAACTCCAGTGTTTCCTTGGCCATCGTCCAA
  GCTGGTCAGAAGGACCAGGGCCTGTATTACTGCTGCCTCAAGAACAGTTATGGAAAAGTCACTGCTGAGT
  TTAACCTCACAGCTGAAGTTCTCAAACAGCTTTCAAGTCACACAGAATATAGAGGATGTGAAGAGATTGA
  ATTCAGCCAGCTCATCTTCAAAGAAGATGTTTTCAATGACAGCTACTTCGGGGACCACCTACGTGGCCAG
  ATCTCCACGGAGGAGCTTCACTTTGGCGAAGGGGTGCACCGCAAAGCTTTCCGGAGCAAGGTGATGCAGG
  GCCTCATGCCGGTCTTCCAGCCCGGCCACGCATGCGTACTCAAGGTGCACAATGCCGTCGCCCATGGGAC
  CAGAAACAATGACGAACTTGTGCAGAGGAACTACAAACTGGCTGCCCAGGAATGCTACGTCCAGAATACT
  GCCAGATACTACGCCAAGATCTACGCCGCTGAAGCACAGCCTCTGGAAGGCTTCGGAGAGGTGCCGGAGA
  TCATTCCTATTTTCCTTATCCATCGGCCCGAGAACAACATCCCATATGCCACAGTGGAAGAAGAGCTGAT
  TGGAGAATTCGTGAAGTATTCCATCCGGGACGGGAAGGAAATCAACTTCCTCAGACGAGATTCAGAGGCT
  GGCCAGAAATGTTGCACCTTCCAGCACTGGGTATACCAGAAAACAAGTGGCTGTCTCCTGGTCACGGACA
  TGCAGGGTGTGGGAATGAAGTTAACTGACGTTGGCATAGCAACACTAGCTAGAGGGTACAAAGGATTTAA
  GGGCAACTGTTCCATGACCTTCATTGATCAGTTCAGAGCGCTGCATCAGTGTAACAAGTACTGTAAAATG
  CTGGGGCTGAAATCCCTTCAAAACAACAGCCAGAAACCCAAGAAGCCCATCGTCGGGAAAGGCAGGGTTC
  CGACAAACGCCACGCAGGTGAAGACGCCTGAGTCTGAGACGCCGCCCGCAGAAAGAAAAACC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR218950 representing NM_001037294
  Red=Cloning site Green=Tags(s)
  
  MTDPGCPERRTLCFLSTLLSQKVPEKSDVVLRCMIAGQPKPEVTWYKNGQAIDLGGTVSSYEFFENQYIH
  LLHLSCCTQSDAAVYQVSARNCVGMICCSASLEVQCLQDPQVSPDPGGGRDAAGECKTEIREEDSINHTD
  EKWNPCKKGESTADSFLDKFNHLSSPQIVARGDSGASNSENPQYIKETRQRMGQYNSNNMQENSFNSNNT
  AEKQDVSQLWTVNATVPGLVSDGLGYEESNESVSPSHQTPKVQKYISFSLPLPETTLGPYPEDSNSINMQ
  PGPQVSSEDSDSDYELCPEITLTYTEEFSDDDLEYLECSDVMTDYSNAVWQRSLQGTDRVFLLESDDEEM
  EFNECGLGGCEHFFTEMGCGPQVSGGMWSMNVATGFCSYHSQPQEVRVRSSGTSGHSPLPLHSEMTLTLG
  PHQDETAKMTEPGRAPLPTAPEAVENDCSGIRGETRDNPEAGEEFSGDNLQTMDKVETEASVKPLSGGSD
  KTEVKQGLESLARERTDEKYPGSKKAALRPTRARRPGMKANTKKQLLRDSAPKGTLDLLPKEPTRQPLPG
  SYGQEPTHTEAGAPGWDSHFHAEVCIPLPAEQDSKILRPPADPLSKEEDSSFEGGGALLNKLFEASQIPD
  RTDHLQMQIQETIGESSSLDQMLAFSVPAEESSTFAGATTHSVSNLSEINRENLSLAQYPGLESCPQSLQ
  QEGRPNRDRDLPGALWAESACELSLLEDNEEEESQPPASVALPQGDGVPCREPEGLSDSFPQPTAPSLPL
  ENVGSGSRVREAAGGVGCFEAGDQETCYATMDLLVGAPVDKYLPQEICPEDLELTEGQSEVCDLCSPDKI
  LAVLQTQGSEPPRSTDKRSQDGKSAEGLLFNSTFTWDTAKEASEDAVGETAADVENPPSTFSSTLPYSER
  GFGETQPLCSETISFVKDSEGSYRSSSLSIPAAIDTLASYSSDRECSKEQSAESTANVDCHQVTREMEGI
  STNAAEVHEIKCHSVSVPQDNDFDVGADQVSCEARDEDNSQSLPDDDSQSGRSLSSSTGEATGETLVPAP
  SSAGDHGHFSMPEGQGLCSRALQMDNQPVCQSQAMEGAHSRGLEEHFQEKGSGMKHGIRPQSTSHQVSLS
  ANDFQEILPSIPTMQQETNVEPLEHSLADSREEIECSSDPRTSDLVVAEKTVGEDSHLVVSVPALPDILL
  GEKDDVGLGSWAVGGKVKIITLEAPVFEIWPPELVRHPGYKEAEAGLTMPGRSWALSDILRAGATRSEPG
  ALGGAAWVPSPQADALMALGANRDTWLGAAPDRQANCNCLSSQCLSQPRFLESSVDPVEDKELEVTDSPS
  EVSKTGEMEMPETLNEEQEETQQILRHPAVVNQSVNFPRILESSVDPIDDRGELEGVWPEKPEPSDSSVE
  GNEFIVGNTCQRVDIQPASLQLPHPQDSGEIIPYEHTTNQNRVDGERAEAKTSLPDKAKAEAEAVVWQAQ
  GPGEEGQGIPSVCSMSQTQDGGDRSLGEAGQRGTDETEVISPLSPLSSCLTGVTHTCVKAETNNSTGHIY
  GGSEPRTRQSVIPMKTEKGTIESKCGNHVRSSDDLTNTPCTSSPKGNVTRLSISHGLEELKSEKLQIAET
  KPLNSSDSPTMTLALISGECESEKDPKSLLRRDPCPKGSTLDSGKKSRDQQQKPVAAQVSKAPGDQSAMA
  GSEEGKKKQEASGSGHLTAGIKKKILSRVAALRLRLEEKENSRKNSIVKKTPKFERSLSRTDEKRDPKRA
  PCKAEGKAPVLLKRIQAEMAPEHSGNIKLSCQFSEIHEDSTVCWTKDSKSIAQAKKSAGDNSSVSLAIVQ
  AGQKDQGLYYCCLKNSYGKVTAEFNLTAEVLKQLSSHTEYRGCEEIEFSQLIFKEDVFNDSYFGDHLRGQ
  ISTEELHFGEGVHRKAFRSKVMQGLMPVFQPGHACVLKVHNAVAHGTRNNDELVQRNYKLAAQECYVQNT
  ARYYAKIYAAEAQPLEGFGEVPEIIPIFLIHRPENNIPYATVEEELIGEFVKYSIRDGKEINFLRRDSEA
  GQKCCTFQHWVYQKTSGCLLVTDMQGVGMKLTDVGIATLARGYKGFKGNCSMTFIDQFRALHQCNKYCKM
  LGLKSLQNNSQKPKKPIVGKGRVPTNATQVKTPESETPPAERKT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001037294
• ORF Size: 6432 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001037294.1, NP_001032371.1
• RefSeq Size: 7250 bp
• RefSeq ORF: 6435 bp
• Locus ID: 225638
• UniProt ID: Q91ZB0
• Cytogenetics: 18 E1
• MW: 233.7 kDa
• Gene Summary: Kinases that recognize phosphorylation sites in which the surrounding peptides have an alpha-helical conformation.[UniProtKB/Swiss-Prot Function]