Inpp5j (NM_172439) Mouse Tagged ORF Clone

CAT#: MR218891

Inpp5j (Myc-DDK-tagged) - Mouse inositol polyphosphate 5-phosphatase J (Inpp5j)

Product Images

Circular map for MR218891

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Inpp5j
• Synonyms: Pib5pa; Pipp
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR218891 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGGCCAGACCAGGAGTGGTAGTGCCAGGCCCGGGACCAGGACTGGACTGGGACCCTTGCCTGGGA
  CTCATGGGGTCTTGCAAGCTGAGATACCCTCGAAGAAGGTAAACTCAAGTTTTCAGCTTCCAGCAAAGAA
  CTCGGGCCCAGCATCTTCGGAACCAAGGTTGACCCTGGCACCTGTGGGACCACGAGCAGCTGTGTCACCT
  CCTTCAGAGAGGCCGAGACTGGTTTTGTCATCTCCCCGACCTGTCCTAGCGCCACTGTCCATCGCTGGAG
  AACAGAAAAGGCCTCCGCCCCCTCACAGCTCCAACAGGGCAGCCAAGTCTGTGGGCCAACTGGTAGTATC
  TGCTGCTGCAGCATCCAAGCCTCCACCAGTCGCCTCAGTCTCAATCCTGGCTCCGAAGTCTCTGGGGCAG
  CTAGTAATATCTGCCTCTGCTATGCCAAGGCCATCTCCGGCTCCCCTGGGGTCCGTCCTAACTCCAACTT
  CTAGGGATCAGAAGCAGTTATCACCCACCTCTGTGGGACCTAAGCCAGCATTGGCAACCTCAGGCCTAAG
  CCTAGCGCTGGCCTCTCAGGAGCAGCCCCCGCAGTCCCCCTCCAGTCCTTCCCCTGTGCCGAGTCCCGTT
  CTGTCACCCTCTCAGGAGGGTCACCTGGCTGCAGCATCTGTGACATCTACCCCAGCTTCTGAGAGACAGT
  TGCCGGCTAGACAGAAGGATACCGCAGTTCCCAGGCCCACTCCTCCTGCAGACAAGTGTCTCTACACTCC
  AGAGCGGGCAGCTGGTCCCGCCACCTCTCCACCAAGGGCCCAAGCTTTCTCAGACCCTCGGCTTTCCCCC
  TCTTTCCGCGCGAGACCAGAGGCTCCACGCCACAGCCCAGAGGATCCCGTCTTGCCGCCGCCACCGCAGA
  CCCTGCCCTTGGATGTGAGCCCAGGCCTTCCAGAGTCTGGCACCCGTTCCCCTGGACTTCTGTCCCCTAC
  CTTTCGGCCAGGAATCCCTTCAAGCCAGACTGTGCCCCCACCTCTGCCAAAGCCACCTCGGTCTCCCAGC
  CGCTCCCCGAGTCGCTCTCCCAACCGCTCTCCCTGTTTGCCCCCAGCCCCTGAGGTGGCACTCCCAAAAC
  CTGTCACCCAGGCTGCAGGGTCTGGCAGGTGCCCAAGCCCTAACCTACAGGCACAAGAAAGTCCAGCAGC
  CGCCACCACCACCACCTCACCAACATCTTCTTGGTCAGCTCAGCCTACTTGCAAGAGCGACCCTGGATTC
  CGGATCACTGTGGTTACATGGAATGTGGGGACCGCCATGCCCCCTGATGATGTCACATCTCTTCTTCACC
  TGGGCAGTGGCCACGACAATGATGGTGCAGATATGATTGCCATAGGGTTGCAAGAAGTCAATTCCATGAT
  CAACAAGCGGCTCAAGGATGCTCTCTTTACTGACCAGTGGAGTGAGCTCTTCATGGATGCACTGGGGCCT
  TTCAACTTTGTGCTGGTGAGCACCGTGAGGATGCAGGGCGTCATTCTGCTGCTCTTTGCCAAGTACTACC
  ACCTACCGTTCCTACGGGACGTGCAGACAGACTGTACTCGTACTGGCCTGGGTGGCTACTGGGGTAACAA
  GGGCGGAGTAAGTGTGCGCTTAGCAGCCTTCGGGCACATGCTGTGCTTCCTAAACTGCCACTTGCCCGCA
  CACATGGACAAGGCAGAACAGCGCAAGGATAACTTTCAAACTATCCTTAGCCTCCAGCAATTCCAGGGAC
  CTGGTGCACACGGCATCTTGGATCATGATCTTGTATTCTGGTTTGGGGACCTGAACTTCCGCATTGAGAG
  CTATGACCTACACTTTGTCAAGTTCGCCATAGACAGCAACCAACTCCATCAGCTCTGGGAGAAGGACCAG
  CTCAACATGGCCAAGAACACTTGGCCCATCTTGAAAGGTTTCCAGGAAGGGCCCCTTAACTTTGCTCCCA
  CTTTCAAGTTTGACGTGGGTACCAACAAATATGATACCAGTGCCAAAAAGCGGAAGCCAGCCTGGACAGA
  TCGCATTCTGTGGAAGGTCAAGGCTCCAAGTGGAGGTCCCAGTCCCTCAGGACGAGAGAGTCATAGGCTC
  CAGGTGACCCAGCACAGCTACCGCAGCCACATGGAGTACACTGTCAGTGACCACAAGCCTGTTGCTGCCC
  AGTTCATTCTGCAGTTTGCCTTCAGGGATGATGTTCCTCTGGTGCGGCTGGAGGTAGCCGATGAATGGGC
  AAGGCCAGAGCAGGCCGTGGTGAGGTACCGCGTGGAAACAGTATTTGCCCGAAGCTCCTGGGACTGGATC
  GGCTTGTACCGGGTGGGCTTCCGTCACTGTAAGGACTATGTGGCTTACGTCTGGGCCAAGCATGAGGAAG
  TGGATGGGAATATCTACCAGGTGACCTTCAGTGAGGAGTCACTGCCCAAGGGCCACGGAGACTTCATTCT
  GGGTTACTATAGCCACCACCACAGCATCCTCATTGGCGTCACAGAACCCTTTCAGATCTCGCTGCCTACC
  TCAGAATCTGCCAGCAGCAGCACAGACAGCTCGGGCACCAGCTCGGAGGGGGAGGATGACAGCACTCTGG
  AGCTGCTGGCACCCAAGTCCCGCAGCCCCAGCCCTGGCAAGTCCAAGAGACACCGTAGTCGCAGCCCTGG
  CCTAGCCCGCTTCCCCAGTCTCGCTCTCCATCCCTCGTCCCGTGAACGCCGTGGTGGCAGCCGAAGTCCC
  TCACCCCAGAGCCGCCAACTGCCTCGGGTAGCCCCTGACAGGGGTCACAGTAGTAGCAGCCGAGGTAGTA
  GTGAGGAGGGGCCCTCTGGGCTGCCTGGACCCTGGGCCTTCCCACCCTCTGTGCCTCGAAGCCTGGGCCT
  GCTTCCAGCCTTGCGCCTGGAGACCGTAGACCCTGGTGGTGGAGGCTCCTGGGGAGCTGACCAGGAGGCC
  CCTGACCCTAATAGCCTCTCCCCTAGCCCCCAGGGCCGACTGGGGCTGGAGGAAGGGGGTTTGGGGCCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR218891 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEGQTRSGSARPGTRTGLGPLPGTHGVLQAEIPSKKVNSSFQLPAKNSGPASSEPRLTLAPVGPRAAVSP
  PSERPRLVLSSPRPVLAPLSIAGEQKRPPPPHSSNRAAKSVGQLVVSAAAASKPPPVASVSILAPKSLGQ
  LVISASAMPRPSPAPLGSVLTPTSRDQKQLSPTSVGPKPALATSGLSLALASQEQPPQSPSSPSPVPSPV
  LSPSQEGHLAAASVTSTPASERQLPARQKDTAVPRPTPPADKCLYTPERAAGPATSPPRAQAFSDPRLSP
  SFRARPEAPRHSPEDPVLPPPPQTLPLDVSPGLPESGTRSPGLLSPTFRPGIPSSQTVPPPLPKPPRSPS
  RSPSRSPNRSPCLPPAPEVALPKPVTQAAGSGRCPSPNLQAQESPAAATTTTSPTSSWSAQPTCKSDPGF
  RITVVTWNVGTAMPPDDVTSLLHLGSGHDNDGADMIAIGLQEVNSMINKRLKDALFTDQWSELFMDALGP
  FNFVLVSTVRMQGVILLLFAKYYHLPFLRDVQTDCTRTGLGGYWGNKGGVSVRLAAFGHMLCFLNCHLPA
  HMDKAEQRKDNFQTILSLQQFQGPGAHGILDHDLVFWFGDLNFRIESYDLHFVKFAIDSNQLHQLWEKDQ
  LNMAKNTWPILKGFQEGPLNFAPTFKFDVGTNKYDTSAKKRKPAWTDRILWKVKAPSGGPSPSGRESHRL
  QVTQHSYRSHMEYTVSDHKPVAAQFILQFAFRDDVPLVRLEVADEWARPEQAVVRYRVETVFARSSWDWI
  GLYRVGFRHCKDYVAYVWAKHEEVDGNIYQVTFSEESLPKGHGDFILGYYSHHHSILIGVTEPFQISLPT
  SESASSSTDSSGTSSEGEDDSTLELLAPKSRSPSPGKSKRHRSRSPGLARFPSLALHPSSRERRGGSRSP
  SPQSRQLPRVAPDRGHSSSSRGSSEEGPSGLPGPWAFPPSVPRSLGLLPALRLETVDPGGGGSWGADQEA
  PDPNSLSPSPQGRLGLEEGGLGP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_172439
• ORF Size: 3012 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172439.3, NP_766027.2
• RefSeq Size: 3473 bp
• RefSeq ORF: 3012 bp
• Locus ID: 170835
• UniProt ID: P59644
• Cytogenetics: 11 A1
• MW: 107.5 kDa
• Gene Summary: Inositol 5-phosphatase, which converts inositol 1,4,5-trisphosphate to inositol 1,4-bisphosphate. Also converts phosphatidylinositol 4,5-bisphosphate to phosphatidylinositol 4-phosphate and inositol 1,3,4,5-tetrakisphosphate to inositol 1,3,4-trisphosphate in vitro. May be involved in modulation of the function of inositol and phosphatidylinositol polyphosphate-binding proteins that are present at membranes ruffles (By similarity).[UniProtKB/Swiss-Prot Function]