Cdca2 (NM_175384) Mouse Tagged ORF Clone

CAT#: MR218654

Cdca2 (Myc-DDK-tagged) - Mouse cell division cycle associated 2 (Cdca2), transcript variant 2

Product Images

Circular map for MR218654

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cdca2
• Synonyms: 2610311M19Rik; AI586158
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR218654 representing NM_175384
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGCGAGTTCACACGACAAGCCTCTCTCTGAATCCAAGGAGTGTGTCCTTAATAATTCTGAAAATG
  ACGTTTTTAGATTGGGAACTGAACCGTTTGTGACTCCTCAGAAGCATGTAGCAGATGCGACTCCTAATCT
  TTGCACCCCTGATACCTTTAAGTCACCTTTGGACTTCACCACAGTAACCGTAGAGCAGTTGGGAATTACA
  CCTGAAAGCTTTGTTAAGACCTCTTCCGGAAAGTCAACATCTTCCCTTCAGAAGGCCAGGCGCCGCTCCA
  CTGTTGGTGTGCGGGGCTCTCCAGAAACCAATTGCCTGATCCGATTCATTGCCCAGCAGCGAAACCTAAA
  GAAGGCTGTGCTCTCTCCGTTGGCACGAGAGCCCCACTTCGAGGGTAGCCCAAGACTGTATCGAAATGCC
  AGTGTTTTAAGAGAGCGAATGTCTGCTTTTCGGTCAGCTTTTCACTCCATACAGGAAACTAAGATGGCCA
  GCAGTCCCTCAGCAGCAGAGGCAGACGGAGAGTCTAGGATATCAGATTTGACCAGAAAAGAAGATCTTCT
  TGAATATCAGCAGTCTGGGTTCCCTGTAAACTCCTCTTCAAAGCGGAGGAGAATATCCTCCCAGGACAGC
  CCTGACAATTATCTCAGTGGCACCAAAGCCCTTGCTGACGAAGCGTGTGCTGGGGGTGCCTCCACAGATC
  TTGCTGAGAAGTCACCTGACATCGGTTCCGCCCAGCCGGGATGCATGGCTGCTCCCCTTCCAGAGCTCAG
  GGAGACTTCCCAGGGACTTGCAGTGACTGACTGTGTAGAGGGCCCTGTAACACCACTGAGCTCAGGCACA
  GCCACTGCCACCAGGTCTCCAGAGACTCCTATGTGCGGGAGCAGCAGCCCCTCCGCTAAGACCACCGCCA
  CCAGGTCTCCAGCGACTCCTGTATGTGGAAGCAGCACCCCCTCCGCTAAGACCTTTGTGCTTCGTTCTGT
  GTTGAAGAAACCTGGTAAGCTGTTCTCAGAGAATGGAAAGGAATCCAACCTCTGTGATGACGGGGCTCAT
  CTGATCTCATATCCTTCAAACTCTTGCAAGGAAGGAAGAGCAGGTAGAGAAAACTGTAAAACACCAGGCT
  GTCTGAATCCGAGGAAGAGGAAGAGAGTTACTTTTGGAGAGGACTTAAGCCCTGAAGTGTTTGATGAGTC
  TTTACCAGCCAATACTCCATTGTGCAAAGGAGGAACACCTGTTCGCCCAAGGACCGTAAAAACTACCAGT
  CCCCTGCAGTCCCCCGTTCATGAGCAGTTTCTTCAGCCAAACTTTGATGACAAGGAGGAGAACCTTGAAA
  ACATAGAGCCACCTCAGGGATCATTTGCAAATCTGAGTCTTAGTAAATCCTCACTCTCTGAGACTCCTCC
  AGGCACTAACACTTGCAGTTCTTTAAATAAAGATGAAGAAATAATCTGCAGTATTGTTAGACCTACACGG
  ACCTCTCAAAGAAGAAAGCAGACGCTGAGTTCTACAGGTGTTTGCAGCTCATATACTACACAAGCTGAGC
  CCCGTAAAGAGAAGATGAGCAGGAGGAAATCTCGAGAAAAAAAGCACACAAGCGCGGCACTTCCTAAAAA
  GAAACAGGTTTTAAAAAGTTACAGAAAAAAGAAAAAGGGGAAAAAAGATGTTGAAAAATGCTTTTATGGG
  CCAAGAGACATTGCATCGAAGAAGCCACTCCTGAGCCCTATTCCTGAGCTGCCTGAAGTCTCTGAAGCGA
  CTCCTCTGGCTGACTGCACGCAGGGGACGAGCTCAGATGATTTCAACAAATGTGGTCAGCTGGAAGAAGT
  GAACTCGTTTGAAATTCCCACACAACGGAAGAGACGCTTACCACAGAAGGCAGATTCGCCTGAGCTGGAC
  CCAGCTCATCATCAGTCTCAGGTCTCTGACAAGTGCTGCTACCTGTTGCCTCTCACTACTGCTTCTGAGC
  GAGGGCCAAATGCCAGTACCAGAGACACAGGCAGTGAAGGGAACACCAGAGCGGAGAGTAAGTGTCAAAG
  TGCAAAGGAGCCAAAACCCGGGACCAAGATGGAAAGCGGTCTTGTGCCTCGGGCTTCTGTGACCCAAGAT
  CACATCGTGTCCAAGAATCCAAAACCTCTGGGCAGCCCTCAGAGCCAGGACCTTTTCAAGGCTGGTCAAA
  ATTTGGAAAATCCTTGTGAGATCCTTATTGTTTCAGAAAGTATGAATCTAAAGTGTGAGAAAGAGAGTGA
  ATGCTTAGCTCCACAGGGGAGTCTGCAGGGCAGCCCTGTCTCTACTGACTCAAAAAGAGACCTTAACTGT
  TCAGAAGATGTCCTCATTCAAAATATAAAAGAACCCGCAAGCCACAGTGAGAATGTGGGAAGGAAATGTG
  CAGGGAATGGCAGCCCTGGCAGTGGGAGAGAGAGGAAATGGAGAAGACGCACAGTGTGTTGTGGGGGTCA
  GAGTTCATACCTGGAACAAAATGGGAACCCTGCGTCTTCCTGCAGTGGGGAAAACTTTGTGGAAATTAGT
  TTAGAAAGCGTGCAGCTGATTGAAGAGCTGTCTAACACCATAGAGCAAAGCTTTCAGAGAACAAGCAGTA
  AAACCAAGGTGAGACGCAGCACAAGGCTCCAGAGAGACTTGGAAAATACAGGGCTTGTGTGGCTGTCTCC
  AAGTCCTTCCACTTTGCAGAAGCCCAGAAGGAGAATGACGATCTGCACCTTAGACAGCAGGGGATTTGAA
  TGTCCATCCTCCAAAGAAGAGACCATATCCTCAGGACAAAACCCAGGGCCGCTGCCCGCTGTCTCGGGCA
  GTGAGAGCCAGGGTGTTGGTTCTTCTGCGCTACCTAGGAAGAGGAGAAGCCTTTGTGGGTCTACCCTCAC
  AGATGCTAACAGTGCCACCCAGCCACCAGACTGCAAGAGAAAACCCTCGCTCAAGGGAGAAAGCGCTCAG
  CTGCCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR218654 representing NM_175384
  Red=Cloning site Green=Tags(s)
  
  MDASSHDKPLSESKECVLNNSENDVFRLGTEPFVTPQKHVADATPNLCTPDTFKSPLDFTTVTVEQLGIT
  PESFVKTSSGKSTSSLQKARRRSTVGVRGSPETNCLIRFIAQQRNLKKAVLSPLAREPHFEGSPRLYRNA
  SVLRERMSAFRSAFHSIQETKMASSPSAAEADGESRISDLTRKEDLLEYQQSGFPVNSSSKRRRISSQDS
  PDNYLSGTKALADEACAGGASTDLAEKSPDIGSAQPGCMAAPLPELRETSQGLAVTDCVEGPVTPLSSGT
  ATATRSPETPMCGSSSPSAKTTATRSPATPVCGSSTPSAKTFVLRSVLKKPGKLFSENGKESNLCDDGAH
  LISYPSNSCKEGRAGRENCKTPGCLNPRKRKRVTFGEDLSPEVFDESLPANTPLCKGGTPVRPRTVKTTS
  PLQSPVHEQFLQPNFDDKEENLENIEPPQGSFANLSLSKSSLSETPPGTNTCSSLNKDEEIICSIVRPTR
  TSQRRKQTLSSTGVCSSYTTQAEPRKEKMSRRKSREKKHTSAALPKKKQVLKSYRKKKKGKKDVEKCFYG
  PRDIASKKPLLSPIPELPEVSEATPLADCTQGTSSDDFNKCGQLEEVNSFEIPTQRKRRLPQKADSPELD
  PAHHQSQVSDKCCYLLPLTTASERGPNASTRDTGSEGNTRAESKCQSAKEPKPGTKMESGLVPRASVTQD
  HIVSKNPKPLGSPQSQDLFKAGQNLENPCEILIVSESMNLKCEKESECLAPQGSLQGSPVSTDSKRDLNC
  SEDVLIQNIKEPASHSENVGRKCAGNGSPGSGRERKWRRRTVCCGGQSSYLEQNGNPASSCSGENFVEIS
  LESVQLIEELSNTIEQSFQRTSSKTKVRRSTRLQRDLENTGLVWLSPSPSTLQKPRRRMTICTLDSRGFE
  CPSSKEETISSGQNPGPLPAVSGSESQGVGSSALPRKRRSLCGSTLTDANSATQPPDCKRKPSLKGESAQ
  LP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_175384
• ORF Size: 2946 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_175384.4, NP_780593.3
• RefSeq Size: 3568 bp
• RefSeq ORF: 2949 bp
• Locus ID: 108912
• UniProt ID: Q14B71
• Cytogenetics: 14 D1
• MW: 106.4 kDa
• Gene Summary: Regulator of chromosome structure during mitosis required for condensin-depleted chromosomes to retain their compact architecture through anaphase. Acts by mediating the recruitment of phopsphatase PP1-gamma subunit (PPP1CC) to chromatin at anaphase and into the following interphase. At anaphase onset, its association with chromatin targets a pool of PPP1CC to dephosphorylate substrates (By similarity).[UniProtKB/Swiss-Prot Function]