Bcam (NM_020486) Mouse Tagged ORF Clone

CAT#: MR218632

Bcam (Myc-DDK-tagged) - Mouse basal cell adhesion molecule (Bcam)

Product Images

Circular map for MR218632

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Bcam
• Synonyms: 1200005K12Rik; B-CAM; Gplu; Lu
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR218632 representing NM_020486
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAACCCCCTGACGCCCGCGCAGGGCTGCTGTGGCTCACCTTCCTGCTGTCGGGCTACTCAGGTGCCC
  AGGCTGAGCTGCACGTGTCCGTGCCCCCACGGGTGGAGGTGATGCGAGGCGAGCAAGTCGCCCTGGACTG
  CACTCCACGGGAGCACCCTGAGCATTACGTGCTGGAATGGTTCCTCGTTGATGGTACTGGGGCTCGACAC
  CGTCTGGCTTCTGTGGAACCACAGGGCTCAGAGTTCCTGGGCACAGTCCACTCTCTGGGCCGCGTACCCC
  CATACGAGGTAGACTCTCGTGGGCGCCTGGTGATAGCAAAGGTCCAGGTGGGCGATGGACGGGACTACGT
  GTGCGTAGTGAAGGCTGGGGCAGCGGGTACCTCAGAGGCCACCTCAAGTGTCCGTGTGTTTGCAACACCA
  GAGGATACGGAGGTGTCTCCCAACAAAGGGACGCTGTCTGTGATGGACCAGTTTGCCCAGGAGATTGCCA
  CCTGCAGCAGCAACAACGGAAACCCCGTGCCTCGGATCACATGGTACCGAAACGGGCAGCGTCTGGAAGT
  ACCCATGGAGGTGAACCAAAAGGGCTACATAACCATCCGTACTGTCCGGGAAGCCTCAGGCCTGTACTCC
  CTGACCAGCACCCTCTACCTGAGGCTGCACAAGGATGATCGGGATGCCAACTTCCACTGTGCTGCTCACT
  ACGACTTGCCCTCAGGCCAACATGGCCGCCTGGACAGCCATACCTTTCGCCTCACCCTGCACTATCCCAC
  GGAACATGTAGAATTCTGGGTGGGGAGCCCGTCCACCACGGAGGGCTGGGTCCGTGAGGGTGATGCTGTC
  CAGCTGCTCTGCCAGGGCGATGGCAGCCCCAGCCCAGAGTATTCTTTTTTCCGCCAGCAGGGTACCCAGG
  AGGAACAGCTGAATGTCAACCTCAAGGGAAACTTGACCCTAGAACGGGTGCATCGGAACCAGAGCGGGAT
  CTACGGCTGCAGAGTGGAGGATTACGATGCCGACGAGGAAGTGCAACTTGTCAAGAAGCTAAAGCTGCAC
  GTGGCCTACCTGGATCCATTGGAGCTGAGCGTGCCTGAGGAGCTCTTCGTATTCCTGAACAGCAGCAGCA
  CGGTGGTGAACTGCTCCGCTAGAGGCCTGCCTACTCCTACTGTGCGATGGACCAAGGATTCTGTGACCCT
  TGCAGACGGCCCCATGCTCTCTCTCCAGTCTGTGACCTTTGACTCAGCTGGCACCTATACCTGTGAGGCG
  TCCACACCCACAGTCCCCCTCCTGAGCCGCACACAGAGTTTCCAGCTCATCGTCCAAGGAGCACCAGAGT
  TAAAACCGAATGAGATAATGCCCAAATCAGGGAATAGCTGGACAGAAGGCGATGAAGTCATGCTGACCTG
  CTCCGCCCGGGGCTTCCCAGAGCCCAAACTCACCTGGAGTCAGCGAGGCGATACTCCAGCAGAGCCGCCC
  TTTGAGGGTAGAGGCTGGAAGAGCAGCTCGCTGATGGTGAAAGTGACCAGCGCCCTGAGCCGAGAGGGCG
  TGTCCTGTGAAGCGTCTAACATCCATGGCAAGAAAGGCCATGTCTTCCACTTTGGCTCTGTGGCCCCTCA
  GACTGCCCAGGCTGGAGTGGCTGTCATGGCTGTAGCTGTCAGCGTCGGTCTTTTGCTACTCGTGGTCGCT
  GCTTTCTATTGCATGAGACGCAAAGGGCGCCCTGGCTGCTGCCGGCGAGCAGAGAAAGGGGCGCCGCCAG
  CCAGGGAACCGGAGTTGAGCCACTCTGGGTCAGAGCGGCCAGAACACACGGGCCTTCTCATGGGTGGGCC
  CTCCGGAGGAGGCAGAGGTGGCAGTGGAGGCTTTGGAGATGAGTGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR218632 representing NM_020486
  Red=Cloning site Green=Tags(s)
  
  MEPPDARAGLLWLTFLLSGYSGAQAELHVSVPPRVEVMRGEQVALDCTPREHPEHYVLEWFLVDGTGARH
  RLASVEPQGSEFLGTVHSLGRVPPYEVDSRGRLVIAKVQVGDGRDYVCVVKAGAAGTSEATSSVRVFATP
  EDTEVSPNKGTLSVMDQFAQEIATCSSNNGNPVPRITWYRNGQRLEVPMEVNQKGYITIRTVREASGLYS
  LTSTLYLRLHKDDRDANFHCAAHYDLPSGQHGRLDSHTFRLTLHYPTEHVEFWVGSPSTTEGWVREGDAV
  QLLCQGDGSPSPEYSFFRQQGTQEEQLNVNLKGNLTLERVHRNQSGIYGCRVEDYDADEEVQLVKKLKLH
  VAYLDPLELSVPEELFVFLNSSSTVVNCSARGLPTPTVRWTKDSVTLADGPMLSLQSVTFDSAGTYTCEA
  STPTVPLLSRTQSFQLIVQGAPELKPNEIMPKSGNSWTEGDEVMLTCSARGFPEPKLTWSQRGDTPAEPP
  FEGRGWKSSSLMVKVTSALSREGVSCEASNIHGKKGHVFHFGSVAPQTAQAGVAVMAVAVSVGLLLLVVA
  AFYCMRRKGRPGCCRRAEKGAPPAREPELSHSGSERPEHTGLLMGGPSGGGRGGSGGFGDEC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020486
• ORF Size: 1866 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020486.2, NP_065232.1
• RefSeq Size: 2407 bp
• RefSeq ORF: 1869 bp
• Locus ID: 57278
• UniProt ID: Q9R069
• Cytogenetics: 7 A3
• MW: 68.1 kDa
• Gene Summary: Laminin alpha-5 receptor. May mediate intracellular signaling (By similarity).[UniProtKB/Swiss-Prot Function]