Bicd1 (NM_001112796) Mouse Tagged ORF Clone

CAT#: MR218586

Bicd1 (Myc-DDK-tagged) - Mouse bicaudal D homolog 1 (Drosophila) (Bicd1), transcript variant 1

Product Images

Circular map for MR218586

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Bicd1
• Synonyms: B830009D06Rik; bic-D 1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR218586 representing NM_001112796
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGCAGAGGAGGCGCTGAAGACGGTAGACCAATATAAGACTGAAATAGAGAGACTGACCAAGGAGC
  TCACAGAAACGACCCATGAGAAGATCCAGGCTGCAGAGTATGGGCTGGTGGTGCTGGAGGAGAAACTGAC
  TCTCAAGCAACAGTATGACGAGCTGGAGGCCGAGTATGACGGCCTCAAACAAGAGCTGGAGCAGCTCAGA
  GAGGCATTTGGGCAGTCCTTCTCTATCCACCGTAAAGTAGCTGAGGATGGAGAGACTCGGGAGGAAACAC
  TTCTTCAGGAGTCTGCATCCAAAGAAGCTTACTATCTAAACAAGATCTTGGAGATGCAGAATGAGCTGAA
  GCAGAGCCGGGCTGTGGTTACAAATGTCCAGGCAGAAAATGAGAGGCTCTCGGCCGTTGTACAGGAACTG
  AAAGAGAACAACGAGATGGTGGAGCTACAGAGAATACGAATGAAAGATGAAATCCGAGAATATAAATTCC
  GGGAGGCCCGACTCCTTCAGGACTACACAGAATTGGAAGAAGAAAATATCACATTGCAGAAACTAGTGTC
  CACATTGAAGCAGAACCAGGTTGAATACGAAGGCTTAAAGCATGAAATTAAGCGATTTGAAGAAGAAACA
  GTATTACTGAATAGCCAACTAGAAGATGCCATTCGACTGAAAGAGATTGCAGAGCATCAGTTGGAAGAAG
  CCCTTGAGACACTGAAGAATGAAAGAGAGCAAAAGAACAACCTGAGGAAGGAGCTGTCCCAGTACATCAA
  CCTCAGTGATAGCCACATCAGCATCTCAGTAGATGGGCTCAAGTTTGCTGAGGATGGAAGTGAGCCAAAT
  AACGATGACAAGATGAATGGACATATCCATGGGCCTCTTGGGAAACTGAATGGAGACTATCGTACTCCCA
  CTACCAGGAAAGGAGAGTCCCTACACCCTGTGTCTGACTTATTCAGTGAGTTGAACATTTCAGAAATACA
  GAAATTGAAGCAGCAGCTTATTCAGGTAGAGAGGGAAAAAGCTATTCTTCTGGCCAACCTCCAGGAGTCA
  CAAACCCAGCTAGAACATACCAAGGGGGCACTGACAGAGCAGCATGAGCGAGTGCACCGGCTCACAGAGC
  ATGTCAATGCTATGAGGGGCTTGCAAAACAGCAAAGAGATCAAGGCTGAGCTAGATTGTGAGAAGGGCCG
  GAACTCAGCGGAGGAAGCTCATGACTATGAGGTGGACATCAATGGCTTAGAGATCCTTGAGTGCAAATAT
  AGAGTGGCCGTCACAGAAGTCATTGATTTGAAAGCAGAAATTAAGGCCTTAAAGGAAAAATATAATAAAT
  CTGTAGAAAATTATACAGAAGAAAAGACCAAATATGAGAGTAAGATCCAAATGTATGATGAACAAGTGAC
  AAACCTTGAGAAGACATCTAAGGAGAGTGGTGAGAAGATGGCCCACATGGAGAAGGAGTTGCAAAAGATG
  ACTGGCATAGCCAACGAAAATCACAACACCCTCAATACAGCCCAGGATGAGTTGGTGACATTTAGTGAGG
  AACTAGCCCAGCTTTACCACCATGTGTGTCTATGTAATAATGAAACTCCCAATAGAGTCATGTTGGACTA
  CTATAGACAAAGCAGAGTTACTCGAAGTGGCAGCCTCAAGGGGCCTGATGATCCCAGAGGGCTTTTGTCG
  CCAAGATTATCCAGGAGGGGTGTGTCATCCCCTGTAGAATCAAGGACATCATCTGAACCAGTTTCAAAGG
  AAAATACAGAGACTAGTAAAGAGCCAAGTCCAACTAAGACTCCCACAATCTCTCCTGTTATTACTGCCCC
  ACCATCATCTCCAGTTTTGGATACCAGTGATATTCGCAAAGAGCCAATGAATATCTACAACCTCAATGCC
  ATAATCCGGGACCAAATCAAGCATCTGCAGAAGGCAGTGGACAGGTCCTTACAGCTGTCTCGTCAAAGAG
  CAGCAGCCCGAGAGTTGGCCCCCATGATTGATAAGGACAAGGAAGCCTTAATGGAAGAGATTCTCAAACT
  GAAGTCCTTGCTGAGCACCAAACGGGAGCAGATTGCGACACTGAGGGCAGTGTTGAAAGCCAATAAGCAG
  ACAGCTGAAGTGGCTTTAGCTAACCTGAAGAACAAATATGAAAATGAAAAGGCAATGGTTACTGAAACAA
  TGACAAAACTGAGGAATGAACTAAAGGCTTTGAAGGAAGATGCTGCAACCTTCTCATCCCTGAGAGCAAT
  GTTTGCAACAAGATGCGATGAATATGTCACACAGTTGGATGAGATGCAGAGACAGTTAGCAGCTGCAGAA
  GATGAGAAGAAGACTCTAAACACTTTATTGCGAATGGCTATCCAGCAAAAACTCGCCCTGACACAGAGGC
  TGGAGGACTTAGAGTTTGACCATGAGCAGTCCCGACGCAGCAAAGGCAAACTTGGAAAGAGCAAGATCGG
  CAGCCCTAAATCCGGACATTGCCCTCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR218586 representing NM_001112796
  Red=Cloning site Green=Tags(s)
  
  MAAEEALKTVDQYKTEIERLTKELTETTHEKIQAAEYGLVVLEEKLTLKQQYDELEAEYDGLKQELEQLR
  EAFGQSFSIHRKVAEDGETREETLLQESASKEAYYLNKILEMQNELKQSRAVVTNVQAENERLSAVVQEL
  KENNEMVELQRIRMKDEIREYKFREARLLQDYTELEEENITLQKLVSTLKQNQVEYEGLKHEIKRFEEET
  VLLNSQLEDAIRLKEIAEHQLEEALETLKNEREQKNNLRKELSQYINLSDSHISISVDGLKFAEDGSEPN
  NDDKMNGHIHGPLGKLNGDYRTPTTRKGESLHPVSDLFSELNISEIQKLKQQLIQVEREKAILLANLQES
  QTQLEHTKGALTEQHERVHRLTEHVNAMRGLQNSKEIKAELDCEKGRNSAEEAHDYEVDINGLEILECKY
  RVAVTEVIDLKAEIKALKEKYNKSVENYTEEKTKYESKIQMYDEQVTNLEKTSKESGEKMAHMEKELQKM
  TGIANENHNTLNTAQDELVTFSEELAQLYHHVCLCNNETPNRVMLDYYRQSRVTRSGSLKGPDDPRGLLS
  PRLSRRGVSSPVESRTSSEPVSKENTETSKEPSPTKTPTISPVITAPPSSPVLDTSDIRKEPMNIYNLNA
  IIRDQIKHLQKAVDRSLQLSRQRAAARELAPMIDKDKEALMEEILKLKSLLSTKREQIATLRAVLKANKQ
  TAEVALANLKNKYENEKAMVTETMTKLRNELKALKEDAATFSSLRAMFATRCDEYVTQLDEMQRQLAAAE
  DEKKTLNTLLRMAIQQKLALTQRLEDLEFDHEQSRRSKGKLGKSKIGSPKSGHCPQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001112796
• ORF Size: 2478 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001112796.2
• RefSeq Size: 5217 bp
• RefSeq ORF: 2481 bp
• Locus ID: 12121
• UniProt ID: Q8BR07
• Cytogenetics: 6 G3
• MW: 95.3 kDa
• Gene Summary: Regulates coat complex coatomer protein I (COPI)-independent Golgi-endoplasmic reticulum transport by recruiting the dynein-dynactin motor complex.[UniProtKB/Swiss-Prot Function]