Trim39 (NM_178281) Mouse Tagged ORF Clone

CAT#: MR218360

Trim39 (Myc-DDK-tagged) - Mouse tripartite motif-containing 39 (Trim39), transcript variant 2

Product Images

Circular map for MR218360

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Trim39
• Synonyms: 1100001D15Rik; E130103K13Rik; mKIAA4179; RBCC-B30.2; Rnf23; tfp
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR218360 representing NM_178281
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGAGACAAGTCTGTTAGAGGCTGGAACCTCTGCAGCCTCCACAGCCGCTGCTCTGGAGAACCTGC
  AGGTGGAAGCAAGCTGTTCTGTATGCCTAGAGTATCTGAAGGAGCCAGTTATTATTGAATGTGGGCACAA
  CTTCTGCAAAGCGTGCATTACCCGCTGGTGGGAAGACCTAGAGCGGGACTTCCCTTGCCCTGTCTGTCGG
  AAGACATCCCGATACCGGAGTCTCCGGCCTAACCGACAGCTAGGCAGTATGGTGGAAATCGCCAAGCAGC
  TCCAGACGGTCAAGCGGAAGATCAGAGATGAAAGCCTCTGCTCCCAGCACCACGAACCTCTTAGCCTTTT
  CTGCTATGAAGACCAGGAGGCTGTCTGTCTGATATGTGCAATTTCTCATACCCACCGGCCCCACACCGTC
  GTGCCGATGGATGATGCTACACAGGAATACAAGGAGAAACTTCAGAAGTGCCTGGAGCCCCTGGAACAGA
  AGCTGCAGGAGATCACCTGCTGCAAAGCCTCCGAAGAGAAGAAGCCGGGGGAGCTCAAGAGACTGGTGGA
  GAGCCGCCGGCAGCAGATCTTAAAGGAGTTTGAGGAGCTGCACAGGCGACTGGACGAAGAGCAGCAGACA
  CTGCTTTCACGACTGGAGGAAGAAGAACAGGACATTCTACAGCGACTTCGAGAAAATGCTGCTCACCTTG
  GGGACAGGCGCCGGGACCTTGCCCACTTAGCTGCTGAGGTGGAGGGCAAGTGCTTACAGTCAGGCTTCGA
  GATGCTTAAGGATGTGAAAAGTACCCTAGAAAAATGTGAGAAGGTGAAGACCATGGAGGTGACTTCAGTA
  TCCATAGAGCTGGAAAAGAACTTCAGCAATTTCCCCCGGCAGTACTTTGCCCTAAGGAAAATTCTTAAAC
  AGCTAATTGCGGATGTGACTCTGGACCCCGAGACTGCTCACCCTAACCTCGTCCTGTCAGAGGATCGGAA
  AAGCGTCAAGTTTGTGGAGACAAGACTCCGAGACCTCCCTGACACACCGCAGCGTTTCACTTTCTACCCT
  TGTGTCTTAGCTACTGAAGGCTTCACCTCAGGTCGACACTACTGGGAGGTGGAGGTGGGTGACAAGACCC
  ACTGGGCAGTAGGAGTATGCCGGGACTCTGTGAGCAGAAAGGGCGAACTGACTCCACTTCCTGAGACTGG
  CTACTGGCGAGTGCGGCTGTGGAATGGGGACAAGTATGCAGCCACCACCACGCCTTTTACCCCTTTGCAC
  ATCAAGGTGAAGCCCAAGCGGGTAGGCATATTCCTAGACTATGAGGCTGGCACACTGTCCTTTTACAACG
  TCACAGACCGTTCACATATCTACACCTTCACTGATACTTTTACTGAAAAACTTTGGCCCCTCTTCTACCC
  AGGGATCCGGGCCGGTCGGAAGAATGCTGCACCACTTACCATTAGGCCCCCAACAGATTGGGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR218360 representing NM_178281
  Red=Cloning site Green=Tags(s)
  
  MAETSLLEAGTSAASTAAALENLQVEASCSVCLEYLKEPVIIECGHNFCKACITRWWEDLERDFPCPVCR
  KTSRYRSLRPNRQLGSMVEIAKQLQTVKRKIRDESLCSQHHEPLSLFCYEDQEAVCLICAISHTHRPHTV
  VPMDDATQEYKEKLQKCLEPLEQKLQEITCCKASEEKKPGELKRLVESRRQQILKEFEELHRRLDEEQQT
  LLSRLEEEEQDILQRLRENAAHLGDRRRDLAHLAAEVEGKCLQSGFEMLKDVKSTLEKCEKVKTMEVTSV
  SIELEKNFSNFPRQYFALRKILKQLIADVTLDPETAHPNLVLSEDRKSVKFVETRLRDLPDTPQRFTFYP
  CVLATEGFTSGRHYWEVEVGDKTHWAVGVCRDSVSRKGELTPLPETGYWRVRLWNGDKYAATTTPFTPLH
  IKVKPKRVGIFLDYEAGTLSFYNVTDRSHIYTFTDTFTEKLWPLFYPGIRAGRKNAAPLTIRPPTDWE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_178281
• ORF Size: 1464 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_178281.1, NP_840065.1
• RefSeq Size: 3070 bp
• RefSeq ORF: 1467 bp
• Locus ID: 79263
• Cytogenetics: 17 B1
• MW: 56.8 kDa
• Gene Summary: E3 ubiquitin-protein ligase. May facilitate apoptosis by inhibiting APC/C-Cdh1-mediated poly-ubiquitination and subsequent proteasome-mediated degradation of the pro-apoptotic protein MOAP1.[UniProtKB/Swiss-Prot Function]