Gcc2 (NM_027375) Mouse Tagged ORF Clone

CAT#: MR218330

Gcc2 (Myc-DDK-tagged) - Mouse GRIP and coiled-coil domain containing 2 (Gcc2)

Product Images

Circular map for MR218330

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Gcc2
• Synonyms: 0610043A03Rik; 2210420P05Rik; 2600014C01Rik; AW112121; mKIAA0336
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR218330 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGATTCTGCTCCAGATGCCGTAGCTGCAGCACCCTCCGGGACCCCGAAATCCAAGCTAGAAACGT
  TGCCCAGAGAAGATCTCATCAAGTTTGCCAAGAAGCAAATGATGCTCCTGCAGAAAGCCAAAGCAAGGTG
  TACAGAATTAGACAAAGAAGTTGAAGAACTCAAATCAAAACCTGTTGATGGAGGAACTGATGATATTATT
  AAGGTACTAACTGAACGCTTGGATGCTCTTCTTCTGGAAAAAGCAGAGACTGAACAACAGTGTCTATGTC
  TGAAAAAAGAAAATGTTAAAATGAAGCAAGAGGTTGAGGATTCTGTAACTAAACTGGAAGAGACACACAA
  AGAGTTTGAACAATCACATAGAAACTACGTAAAAGAAATTGAAAGCTGTAAAAATGAGCTGATGGCAGTG
  CACTCAGAGCACAGCAAAGAAACGGCCATCTTACAAAAAGAACTGGAAGAAGCTGTCCATAAGCAAGTAG
  AGCTGAGAGAGCAGCTTAAGTCTCAGAGTGACTCTGAAGATAACGTGAGAAAACTGCAGGAAGAGATTCA
  AAACATCACAGCAGCATTTGAGGAACAGATTTCATGTCTAGAGAAGAAATTAGAAGCTACCAGTGATGAA
  AAACAACAAGAGATTATTCATCTCCAGAAAGTCATTGAGGACAAGGCTCAGCACTACCAAAAAGATATTA
  ATACTTTCCAAGCAGAGATTTTGCAGCTGAGAGCTACACACAAAGAAGAAGTTACTGAGCTGATGTCTCA
  AATAGAAACATCAGCTAAAGAACACGAGGCAGAAATAAATAAACTAAAAGAGAACAGAGTGACACAGTGT
  GAGGCAAGTGAGAACATTCCAGAGAAATACCAATGTGAATCGGAAAACTTAAATGAAGTCGCCTCAGATG
  CAAGCCCGGAGAGTCAGAATTGCTCTGTGGCCTTACAGGAAGATCCTTCTGCGGAACAAACAGTATGTGA
  TAAAGTCAGACAATTGGAAGATTCATTAAAAGAACTGGAATCTCAACATAGTATCTTAAAAGATGAAGTA
  ACTTATATGAATAATCTCAAGTTAAAACTTGAAATGGATGCCCAGCATATAAAGGATGAGTTTTTCCATG
  AACGAGAAGACTTAGAGTTTAAAATTAATGAACTGTTGCTGGCTAAAGAAGAACAGGGCTATGTAGTAGA
  AAAATTAAAATATGAGCGAGAAGATTTAAATAGGCAACTTTGCTGTGCTGTGGAACAACATAACAAAGAA
  ATACAGCGTCTTCAGGAACACCATCAGAAAGAAGTCTCTGAACTAAGTGAGACATTTATATCAGGTTCAG
  AAAAAGAAAAGTTAGCATTGATGTTTGAAATACAAGGTCTTAAGGAACAGTGTGAAAATCTACAACATGA
  AAAGCAAGAGGTAGTTCTAAACTATGAGAGTTTACGAGAGATGATGGAAATTTTACAAACAGAACTTGGA
  GAATCTGCTGGAAAAATAAGTCAAGAGTTTGAAACAATGAAGCAACAGCAAGCATCTGATGTTCATGAGC
  TTCAGCAGAAGCTAAGAAGTGCTTTTAATGAAAAAGATGCTCTTCTTGAAACTGTGAATCGCCTCCAAGG
  AGAAAATGAAAAATTATTATCTCAACAAGAATTAGTACCAGAACTTGAAAGTACCATAAAGAACCTTCAG
  GCAGATAATAGCATGTACTTAGCCAGTCTTGGTCAAAAAGATACCATGTTACAAGAATTAGAAGCAAAGA
  TAAGTTCTCTTGCTAAAGAAAAAGATGACTTTATAAGTAAAATCAAAACTTCCCATGAAGAGATGGATGA
  TCTCCATCAGAAATGGGAAAGGGAACAGAGACTGTCTGTAGAACTCAGGGAAGCAGCAGGGCAAGCTGCC
  CAGCACAACAGTGAACTGAGACAAAGAGTAAGTGAATTAACAGGGAAACTAGATGAACTAGTAAGAGAAA
  AGAGTCAAAATGACCAAAGCATTACGGTTCAAATGAAAACTATGACGGAAGATCAAGAAGCTCTGTCATC
  TAAAATCAAGTCTCTTTATGAGGAAAACAATAGACTACATTCAGAAAAGGCCCAGTTGAGCAGGGATTTA
  GAAGCACTTCAGGCTCAACAAGATTTTGCCCATAAAGAACATGTTGCTGAATTCGAAAAGAAACTCCAGT
  TAATGGTTGAGGAGCGAGACGATTTAAATAAACTGCTTGAAAATGAGCAAGTTCAGAAGTCATTTGTTAA
  AACTCAGTTGTATGAGTATCTTAAGCAACTGAGGGCAAGCATTTTAGAAGAAAATGAAGAGGAAGATGTT
  GTCAAACTTATACAAGCTGTAGGTGAATCTTTAGTGAAAGTAAAGGAAGAAGAACATAACTTAGTCTTTG
  AATATGATGCAAGGGTATTAGAATTAGAAAATAAGATTAAGTGTCTTCAAGAGGACAGTGCAGTTCAGTG
  TGAAGAACTAAGGACTTTAGTAAGAGACTCTGAGCAAGAGAAAATTCTCCTAAGAAAAGAATTAGATGCA
  GTCACATCAGCAAAAGAGGCCCTCCAACTTGATCTTTTAGAAATGAAGAATACTAATGAGAAAGCAAGCC
  TTGAAAACCAGACTCTTTCAACTCAGGTTGAAGAATTATCTCAGACATTACACAGCAGAAATGAAGTCCA
  TGATGAAAAGGTTCTTGTAATAGAACATGAAAACCTAAGGCTACTGCTTAAACAGAGGGAATCTGAACTC
  CAAGATGTGAGAGCAGAATTGATACTGCTAAAGGATTCCTTAGAGAAATCGCCTTCTGTAAAAAATGATC
  AACTTTCATTGGTAAAAGAGCTGGAAGAAAAAATAGAAAGTCTGGAAAAAGAATCCAAAGACAAGGATGA
  GAAAATAAGTAAGATAAAACTAGTTGCTGTGAAAGCAAAGAAAGAACTAGATTCTAACAGGAAAGAGGCC
  CAGACACTACGGGAAGAACTGGAATCCGTGCGGTCAGAAAAGGACCGCCTGTCTGCTTCTATGAAGGAGT
  TCCTCCAAGGAGCAGAGAGCTATAAGAGTCTTCTGTTAGAATATGATAAGCAGTCAGAACAGCTGGATGT
  GGAAAAAGAACGTGCTCATAATTTTGAACGTCATATAGAAGACCTTACCAAACAATTAAGAAATTCGACT
  TGCCAGTATGAAAGACTAACTTCAGACAACGAGGATCTCCTGGCTCGAATTGAGACCCTGCAGGCTAATG
  CCAAGTTGTTAGAAGCACAGATCTTAGAAGTCCAGAAAGCCAAAGGAGTAGTAGAAAAAGAACTGGACGC
  TGAAAAACTTCAGAAAGAGCAGAAAATTAAGGAACATGTCAGTACTGTAAATGAGCTCGAAGAACTCCAA
  CTCCAATTTCAAAAGGAAAAGAAGCAGCTTCAGAAAACAATGCAAGAATTAGAGCTGGTTAAAAAGGATG
  CCCAACAGACCACATTGATGAATATGGAAATAGCAGATTACGAACGTTTGATGAAAGAACTAAATCAAAA
  GTTAACAAATAAAAACAGTACAATAGAAGATTTGGAGCAAGAAATGAAAATTCAGAAAGAGAAACAAGAA
  ACCCTACAAGAAGAAATAACTTCACTGCAGTCGTCAGTACAGCACTATGAAGAAAAGAACACTAAAATCA
  AGCAGCTGCTTGTGAAAACCAAAAAGGAGCTGGCAGATGCAAAGCAAGCGGAAACCGATCACTTACTACT
  CCAGGCATCTTTAAAGGGTGAGCTGGAAGCAAGCCAGCAGCAAGTAGAAGTCTATAAAATTCAGCTGGCT
  GAGATGACATCTGAGAAACACAAAATCCATGAGCATCTGAAGACTTCTGCGGAACAGCACCAGCGCACAC
  TGAGTGCATACCAGCAGAGGGTGGTGGCCCTGCAGGAGGAGAGTCGCGCTGCCAAGGCAGAACAAGCTGC
  TGTCACCTCTGAATTTGAGAGCTACAAAGTCCGAGTTCATAACGTTCTAAAGCAACAGAAAAATAAATCT
  GTGTCTCAGGTTGAAACTGAGGGAGCTAAACAAGAAAGGGAACACCTGGAAATGCTGATTGACCAACTGA
  AAATCAAGTTACAGGACAGCCAAAATAGCTTACAAATCAGTGTATCGGAGTACCAGACCCTGCAGGCTGA
  GCACGACACTCTGCTAGAGAGGCACAACCGGATGCTGCAGGAAACTGTGACCAAAGAGGCAGAACTTCGG
  GAAAAGCTATGTTCAGTTCAATCTGAGAACACCATGATGAAGTCTGAGCACTCGCAGACAATGTGTCAGC
  TAACATCCCAGAATGAAGCACTCCGTACCAGCTTCCGAGACCAAGTTCGACACTTGCAGGATGAGCACAG
  AAAGACAGTGGAGACTCTGCAGCACCAGCTCTCCAAGCTGGAAGCTCAGCTCTTCCAGCTCAAGAGTGAG
  CCATCCACAAGAAGCCCTGCTTCTTCCCATCAACCTTCGAAAAGCCTCCGAGAAAGAAGAACCACAGACC
  TCCCCCTTCTGGACATGCACACTGTGGCCCGCGAGGAGGGGGAGGGCATGGAGACAACTGACAGCGAGTC
  TGTGTCGTCGGCAGGCACACACATTCAGTCCTTAGAGCAGCTCCTTAGCTCTCCTGACACCAAGCTTGAA
  CGCCTTGCAGAGACCTCCTTGTGGCATAATGAATTTACCAAAGAAGAATTAGCTGAGAAGCTCAGCTCCA
  CCACGAAGAGTGCAGATCACTTAAACGGCCTGCTTCGGGAGACAGAGGCTACCAACGCTATCCTTATGGA
  GCAAATTAAGCTTCTCAAGAGTGAAATAAGAAGACTGGAAAGAAACCAAGAGCGTGAGAAGTCTGTGGCT
  AACCTGGAGTACTTGAAGAATGTCTTGCTGCGGTTCATTTTCCTGAAGCCGGGTAGTGAAAGAGAGAGGC
  TTCTGCCTGTGATAGACACCATGCTGCAGCTCAGCCCTGAGGAAAAGGGAAAGCTTGCCACAGTTGCTCA
  AGGTGAGGAAGAAAGTGCTTCTCGTTCCTCTGGATGGGCGTCCTATCTGCATAGCTGGTCTGGACTGCGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR218330 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEDSAPDAVAAAPSGTPKSKLETLPREDLIKFAKKQMMLLQKAKARCTELDKEVEELKSKPVDGGTDDII
  KVLTERLDALLLEKAETEQQCLCLKKENVKMKQEVEDSVTKLEETHKEFEQSHRNYVKEIESCKNELMAV
  HSEHSKETAILQKELEEAVHKQVELREQLKSQSDSEDNVRKLQEEIQNITAAFEEQISCLEKKLEATSDE
  KQQEIIHLQKVIEDKAQHYQKDINTFQAEILQLRATHKEEVTELMSQIETSAKEHEAEINKLKENRVTQC
  EASENIPEKYQCESENLNEVASDASPESQNCSVALQEDPSAEQTVCDKVRQLEDSLKELESQHSILKDEV
  TYMNNLKLKLEMDAQHIKDEFFHEREDLEFKINELLLAKEEQGYVVEKLKYEREDLNRQLCCAVEQHNKE
  IQRLQEHHQKEVSELSETFISGSEKEKLALMFEIQGLKEQCENLQHEKQEVVLNYESLREMMEILQTELG
  ESAGKISQEFETMKQQQASDVHELQQKLRSAFNEKDALLETVNRLQGENEKLLSQQELVPELESTIKNLQ
  ADNSMYLASLGQKDTMLQELEAKISSLAKEKDDFISKIKTSHEEMDDLHQKWEREQRLSVELREAAGQAA
  QHNSELRQRVSELTGKLDELVREKSQNDQSITVQMKTMTEDQEALSSKIKSLYEENNRLHSEKAQLSRDL
  EALQAQQDFAHKEHVAEFEKKLQLMVEERDDLNKLLENEQVQKSFVKTQLYEYLKQLRASILEENEEEDV
  VKLIQAVGESLVKVKEEEHNLVFEYDARVLELENKIKCLQEDSAVQCEELRTLVRDSEQEKILLRKELDA
  VTSAKEALQLDLLEMKNTNEKASLENQTLSTQVEELSQTLHSRNEVHDEKVLVIEHENLRLLLKQRESEL
  QDVRAELILLKDSLEKSPSVKNDQLSLVKELEEKIESLEKESKDKDEKISKIKLVAVKAKKELDSNRKEA
  QTLREELESVRSEKDRLSASMKEFLQGAESYKSLLLEYDKQSEQLDVEKERAHNFERHIEDLTKQLRNST
  CQYERLTSDNEDLLARIETLQANAKLLEAQILEVQKAKGVVEKELDAEKLQKEQKIKEHVSTVNELEELQ
  LQFQKEKKQLQKTMQELELVKKDAQQTTLMNMEIADYERLMKELNQKLTNKNSTIEDLEQEMKIQKEKQE
  TLQEEITSLQSSVQHYEEKNTKIKQLLVKTKKELADAKQAETDHLLLQASLKGELEASQQQVEVYKIQLA
  EMTSEKHKIHEHLKTSAEQHQRTLSAYQQRVVALQEESRAAKAEQAAVTSEFESYKVRVHNVLKQQKNKS
  VSQVETEGAKQEREHLEMLIDQLKIKLQDSQNSLQISVSEYQTLQAEHDTLLERHNRMLQETVTKEAELR
  EKLCSVQSENTMMKSEHSQTMCQLTSQNEALRTSFRDQVRHLQDEHRKTVETLQHQLSKLEAQLFQLKSE
  PSTRSPASSHQPSKSLRERRTTDLPLLDMHTVAREEGEGMETTDSESVSSAGTHIQSLEQLLSSPDTKLE
  RLAETSLWHNEFTKEELAEKLSSTTKSADHLNGLLRETEATNAILMEQIKLLKSEIRRLERNQEREKSVA
  NLEYLKNVLLRFIFLKPGSERERLLPVIDTMLQLSPEEKGKLATVAQGEEESASRSSGWASYLHSWSGLR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_027375
• ORF Size: 5043 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_027375.2, NP_081651.2
• RefSeq Size: 6528 bp
• RefSeq ORF: 5043 bp
• Locus ID: 70297
• Cytogenetics: 10 B4
• MW: 194.6 kDa