Slc6a18 (NM_001168646) Mouse Tagged ORF Clone

CAT#: MR218259

Slc6a18 (Myc-DDK-tagged) - Mouse solute carrier family 6 (neurotransmitter transporter), member 18 (Slc6a18), transcript variant 6

Product Images

Circular map for MR218259

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Slc6a18
• Synonyms: B0AT3; D630001K16Rik; Xt2; Xtrp2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR218259 representing NM_001168646
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCACAGGCCTCAGGGATGGACCCGCTTGTGGACATTGAGGATGAAAGACCCAAGTGGGACAACAAAC
  TCCAGTACCTCCTGAGCTGCATCGGTTTTGCTGTGGGGCTGGGGAACATATGGAGGTTCCCCTACCTGTG
  CCAGACCCACGGAGGAGGGGCCTTCCTCATCCCCTATTTCATTGCCCTGGTCTTTGAGGGTATCCCGCTT
  TTCTACATCGAGCTTGCCATTGGCCAGCGCCTACGGAGGGGAAGCATTGGAGTGTGGAAGACCATCTCCC
  CTTACCTCGGTGGCGTAGGCCTGGGCTGCTTCTCAGTGTCCTTCCTGGTCAGTTTGTACTACAACACGGT
  TCTCCTGTGGGTCTTATGGTTCTTCCTCAACTCCTTCCAACACCCGCTGCCCTGGAGCACATGTCCGCTG
  GATCTCAACAGAACAGGATTTGTGCAGGAATGCCAGAGCAGTGGCACCGTGAGCTACTTCTGGTACCGGC
  AGACTCTGAATATCACATCTGACATCAGCAACACAGGCACTATCCAATGGAAGCTGTTCCTCTGCCTGGT
  GGCCTGCTGGTCAACTGTGTACCTGTGTGTCATCAGAGGCATTGAGAGCACGGGGAAGGTGATCTACTTT
  ACAGCCTTATTCCCTTACCTGGTGCTAACCATCTTCCTCATCAGAGGTCTTACCCTGCCTGGAGCAACAG
  AGGGCCTGATCTACCTGTTTACTCCCAATATGAAGACTCTTCAGAATCCACGGGTGTGGTTGGATGCAGC
  CACCCAGATTTTCTTCTCCCTGTCCCTGGCCTTTGGAGGGCATATTGCTTTTGCAAGCTACAACCCACCC
  AGGAACAATTGTGAGAAGGACGCCGTGATTATTGCCCTGGTCAACAGCATGACCTCCCTGTATGCATCCA
  TCGCCATCTTCTCCGTCATGGGGTTCAAGGCATCCAATGACTATGGAAGGTGCCTGGACAGAAATATCTT
  GAGCCTCATCAATGAGTTTGACCTTCCAGAGCTTAGCATCTCCAGGGATGAGTACCCATCTGTCCTCATG
  TACCTGAATGCCACTCAGACTGCGAGGGTGGCCCAACTTCCCCTGAAGACCTGCCATCTGGAAGATTTTC
  TGGATAAGAGTGCCTCGGGCCCAGGCCTGGCCTTCATCGTTTTCACAGAAGCTGTCCTGCACATGCCAGG
  TGCTTCTGTGTGGTCTGTGCTCTTCTTTGGGATGCTGTTTACCCTGGGTCTGTCCTCCATGTTTGGGAAC
  ATGGAGGGTGTCATTACACCACTATTGGACATGGGGATCTTACCCAAAGGTATACCCAAGGAGGTCATGA
  CTGCCATTTGCTTCACACTGCAGTCTGGAGGCTACTGGTTGGAGATCTTTGACAGTTTTGCAGCTTCTCT
  GAATTTAATCATCTTCGCCTTCATGGAAGTGGTGGGAGTCATTCACATTTATGGGATGAAACGGTTCTGT
  GATGACATTGAATGGATGACTGGGCGGCGGCCCGGCCTCTACTGGCAGGTGACATGGAGGGTTGTGAGCC
  CTATGCTGCTGTTTGGAATCTTCCTGTCCTACATTGTCCTTCTGATCCAGACACCGCCCAGTTACAAGGC
  CTGGAACCCCCAATATGAACATTTTCCCTCAAGAGAGGAGAAGTTCTACCCAGGCTGGGTGCAGGTCACC
  TGTGTGCTCCTGTCCTTCCTGCCCTCACTGTGGGTCCCTGGAGTTGCTCTGGCTCAGTTACTGTCCCAGT
  ACAAACAGAGGTGGAAGGCTACGCATCTGGAAAGTGGTCTGAAGCTACAGGAGAGCAGAGGCTGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR218259 representing NM_001168646
  Red=Cloning site Green=Tags(s)
  
  MAQASGMDPLVDIEDERPKWDNKLQYLLSCIGFAVGLGNIWRFPYLCQTHGGGAFLIPYFIALVFEGIPL
  FYIELAIGQRLRRGSIGVWKTISPYLGGVGLGCFSVSFLVSLYYNTVLLWVLWFFLNSFQHPLPWSTCPL
  DLNRTGFVQECQSSGTVSYFWYRQTLNITSDISNTGTIQWKLFLCLVACWSTVYLCVIRGIESTGKVIYF
  TALFPYLVLTIFLIRGLTLPGATEGLIYLFTPNMKTLQNPRVWLDAATQIFFSLSLAFGGHIAFASYNPP
  RNNCEKDAVIIALVNSMTSLYASIAIFSVMGFKASNDYGRCLDRNILSLINEFDLPELSISRDEYPSVLM
  YLNATQTARVAQLPLKTCHLEDFLDKSASGPGLAFIVFTEAVLHMPGASVWSVLFFGMLFTLGLSSMFGN
  MEGVITPLLDMGILPKGIPKEVMTAICFTLQSGGYWLEIFDSFAASLNLIIFAFMEVVGVIHIYGMKRFC
  DDIEWMTGRRPGLYWQVTWRVVSPMLLFGIFLSYIVLLIQTPPSYKAWNPQYEHFPSREEKFYPGWVQVT
  CVLLSFLPSLWVPGVALAQLLSQYKQRWKATHLESGLKLQESRGC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001168646
• ORF Size: 1815 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001168646.1, NP_001162117.1
• RefSeq Size: 4284 bp
• RefSeq ORF: 1818 bp
• Locus ID: 22598
• UniProt ID: O88576
• Cytogenetics: 13 40.13 cM
• MW: 68.6 kDa
• Gene Summary: Functions as a sodium and chloride-dependent neutral amino acid transporter in kidneys (PubMed:26240152, PubMed:19478081). Required CLTRN for cell surface expression and for its amino acid transporter activity (PubMed:26240152).[UniProtKB/Swiss-Prot Function]