P4ha2 (NM_011031) Mouse Tagged ORF Clone

CAT#: MR218159

P4ha2 (Myc-DDK-tagged) - Mouse procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha II polypeptide (P4ha2), transcript variant 2

Product Images

Circular map for MR218159

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: P4ha2
• Synonyms: AA407196; C76437; P4hl
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR218159 representing NM_011031
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGCTCCAGGTGTTGGTGTTGGTGTTGCTGATGTCCTGGTTCGGTGTCCTGAGCTGGGTGCAGGCAG
  AATTCTTCACCTCCATTGGGCACATGACCGATCTGATTTACGCAGAGAAGGACCTGGTACAGTCTCTGAA
  GGAGTACATCCTTGTGGAGGAAGCCAAGCTCGCCAAGATTAAGAGCTGGGCCAGCAAGATGGAAGCCCTG
  ACCAGCAGATCAGCTGCCGACCCCGAGGGCTACCTGGCTCATCCTGTGAATGCCTACAAGCTGGTGAAGC
  GGTTGAACACAGACTGGCCTGCACTGGGGGACCTTGTCCTTCAGGATGCTTCGGCAGGTTTTGTCGCTAA
  CCTCTCAGTTCAGCGGCAATTCTTCCCCACTGATGAGGACGAGTCTGGAGCTGCCAGAGCCCTGATGAGA
  CTTCAGGACACGTACAAACTGGATCCGGACACGATTTCCAGAGGGGAACTTCCAGGCACAAAGTACCAGG
  CCATGCTGAGTGTGGACGACTGCTTTGGGCTGGGCCGCTCAGCTTACAATGAAGGAGACTATTACCATAC
  TGTGCTGTGGATGGAGCAGGTACTGAAGCAGCTCGATGCTGGGGAGGAGGCCACTGTTACCAAGTCCCTG
  GTGCTGGACTACCTGAGCTATGCTGTCTTCCAACTGGGTGACCTGCACCGTGCTGTGGAACTCACCCGCC
  GCCTGCTCTCTCTTGACCCAAGCCACGAACGAGCTGGAGGGAATCTGCGGTACTTTGAACGGTTGTTAGA
  GGAAGAAAGAGGGAAATCACTGTCAAATCAGACAGACGCCGGACTGGCCACCCAGGAAAACTTGTACGAG
  AGGCCCACGGACTACCTGCCTGAGAGGGATGTGTACGAGAGCCTGTGTCGAGGGGAGGGCGTGAAACTGA
  CACCCCGGAGGCAGAAGAAGCTTTTCTGTAGGTACCATCATGGAAACAGAGTGCCACAGCTCCTCATCGC
  CCCCTTCAAAGAGGAAGACGAGTGGGACAGCCCACACATCGTCAGGTACTATGATGTGATGTCCGACGAA
  GAAATCGAGAGGATCAAGGAGATTGCTAAGCCCAAACTTGCACGAGCCACTGTGCGTGACCCCAAGACAG
  GTGTCCTCACTGTTGCCAGCTACAGAGTTTCCAAAAGCTCCTGGCTAGAGGAGGATGACGACCCTGTTGT
  GGCCCGGGTCAACCGGCGGATGCAACATATCACCGGGCTAACGGTGAAGACTGCAGAGCTATTGCAGGTC
  GCAAACTACGGAATGGGGGGACAGTACGAACCACACTTTGACTTCTCAAGGAGCGATGAGCAAGATGCTT
  TCAAGCGTTTAGGGACTGGGAACCGTGTGGCCACGTTTCTAAACTACATGAGCGATGTCGAAGCTGGTGG
  TGCCACCGTCTTTCCTGACTTGGGAGCTGCTATTTGGCCCAAGAAGGGCACAGCTGTATTCTGGTACAAC
  CTTCTTCGCAGTGGGGAAGGTGATTATCGGACGAGACATGCAGCCTGCCCTGTGCTTGTGGGCTGCAAGT
  GGGTCTCCAACAAGTGGTTCCATGAGCGAGGACAGGAGTTCTTAAGACCTTGTGGAACAACGGAAGTTGA
  T
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR218159 representing NM_011031
  Red=Cloning site Green=Tags(s)
  
  MKLQVLVLVLLMSWFGVLSWVQAEFFTSIGHMTDLIYAEKDLVQSLKEYILVEEAKLAKIKSWASKMEAL
  TSRSAADPEGYLAHPVNAYKLVKRLNTDWPALGDLVLQDASAGFVANLSVQRQFFPTDEDESGAARALMR
  LQDTYKLDPDTISRGELPGTKYQAMLSVDDCFGLGRSAYNEGDYYHTVLWMEQVLKQLDAGEEATVTKSL
  VLDYLSYAVFQLGDLHRAVELTRRLLSLDPSHERAGGNLRYFERLLEEERGKSLSNQTDAGLATQENLYE
  RPTDYLPERDVYESLCRGEGVKLTPRRQKKLFCRYHHGNRVPQLLIAPFKEEDEWDSPHIVRYYDVMSDE
  EIERIKEIAKPKLARATVRDPKTGVLTVASYRVSKSSWLEEDDDPVVARVNRRMQHITGLTVKTAELLQV
  ANYGMGGQYEPHFDFSRSDEQDAFKRLGTGNRVATFLNYMSDVEAGGATVFPDLGAAIWPKKGTAVFWYN
  LLRSGEGDYRTRHAACPVLVGCKWVSNKWFHERGQEFLRPCGTTEVD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011031
• ORF Size: 1611 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011031.2, NP_035161.2
• RefSeq Size: 2284 bp
• RefSeq ORF: 1614 bp
• Locus ID: 18452
• UniProt ID: Q60716
• Cytogenetics: 11 32.13 cM
• MW: 61.5 kDa
• Gene Summary: Catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins.[UniProtKB/Swiss-Prot Function]