A2ml1 (NM_001001179) Mouse Tagged ORF Clone

CAT#: MR218078

BC048546 (Myc-DDK-tagged) - Mouse cDNA sequence BC048546 (BC048546)

Product Images

Circular map for MR218078

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: A2ml1
• Synonyms: Ovos; Ovos2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR218078 representing NM_001001179
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGCCTACCATCTTGCTCAGTGCCCTCCTGCTTCATTTCACTGATGTTGTGGCTGCAGAACCGCGAT
  ACATCCTGTGGGTTTCTTCCGTGGTACAGCGATTTTCCTCAGAAAAAGCCTGTCTTCATCTTTTAAACCT
  CAACGAATCTGTGTCCTTGAGCGTCACCCTAGAATATGATGGATCCAGTACTACTATTTTTGACCAGCCC
  GTGGACGAGGGGAACTTCTACGCTTGTGCAGATTTCAAAGTGTCTCAGATGTCCTCAGAGCAGTTGGCCT
  TTGTGGCATTACTTGTTCAGGGAAACACTCTTAAAATCTCTGAGAGAAGATCTGTGGCCATCGCTGCTGA
  GGAGAACGCCACTTTTGTGCAGACAGACACACCGGTCCATAAACCTGGAGACACAGTTCACTTTCGCGTT
  GTGACACTGAACATCTGGCTGAAGCCTGTTGATGACTTGTATCCCTTGATCACCGTTCAGGATCCCCAAA
  GCAATGTGATTTTTCAGTGGATCAATGTGACCACTTTCAGAAACATTACCCAGCTCTCCTTCCAACTGAC
  GCCGGAACCAATATTGGGGGATTACACAATCGTCATAAAAACACAATCGGGAACGACAGTGATGGATCAT
  TTCACTGTTAACCGAGACGTTTTGCCCAAATTTGAAGTCGAGCTCACTGCACCAGAGACAATAACCATTG
  CGGACAGTCAATTTCAAATGGTTACATGTGCAAAGTACACCTATGGCCAGCCTGTGCAGGGAAAAGCCCA
  GATAAAAGTGTGCAGAGAGCTCTTCTCCCCGGCACATTGTGAGAGCAATGAGAATGAAATCTGTGAGCAG
  TTCACTGTGCAGCTGAAAGATGGCTGTGCTTCTCACATTATCAACACAAAAGTCTTCCAGTTGGACCGCT
  CTGGATTGTTCATGACCTTGAATGTTAACGAAGTGGTTACAGAATCTGGAACAGGTGTGCAGATGAGTAA
  AACACACTCGGTTTTTATCACTTCCGTGCTTGGCACTGTCAGCTTCGAGAACATGGACCCTTTCTACAGG
  AGAGGGATCACTTATTTTGGAACTCTTAAATTTTCTGGTCCCAATAACACACCACTGGTGGACAAACTTT
  TGCAACTGGAACTCGACGGCAAACCTGTAGGAAATTACACCACAGATGAGAACGGGGAAGCTCGATTTTC
  CATTAACACTTCAGAGATATTCGGTGCACAGATTAGCTTGAAGGCCGTCTACGTCCGACCTAGAAGCTGC
  CATCGTTCCAGCTGGCTGTCCCCTGAGTATTTGGACGCTTACTTCTCGGCCTCGCGCTTTTACTCCCAGA
  CCAGCAGCTTCACGAAGATCATTCTCGAGCCCAAGCAGCTTCCGTGCGATCAAGAGAAGATGTTCTCAGT
  ACTTTACTCCCTAAACCCCGAGGCCTACAAGGAGGCCTCAGATGTGACCTTCTTCTATTTGGTGATGGTG
  AGAGGAGGTATCTCCCGCAGCGGACAGAAGCAAGTCAGAGTTCAAGCCTGGAATGGGAACTTCTCTTTCC
  CAATCAGCATCAATGCTGATCTGGCGCCATCGGCTGACCTGTTCGTCTACACCCTCCACCCTAGCGGGGA
  AATCGTTGCGGATAATGTCAGGCTCCAGATCGAGAAGTGCTTTAAAAACAAGGTTAGCATAAACTTCTCA
  CGGGATAAGGATCTACCGGGCTCTAACACCAGTGTTCATCTTCAAGCGGCCCCTGACTCCTTCTGTGCCC
  TGCGAGCAGTGGACAAAAGTGCCCTTCTACTGAACCACGGACAAGAAATGACACCTGAGAGCGTGTACTT
  CACACTCCCGTATATCCATCAATATGGCTATTTCTACAATGGCCTCAACTTGGATGATCAGCAAGCAGAA
  CCATGCATTCCTCAGAAGGACTTATTCTACAATGGACTGTATTATACACCTACAGGCAATATTTGGGATG
  GAGACCTCTCTAATCTTCTCAGTAACATGGGTCTGAAAATCTTTACAAATCTCCATTACCGGAAACCGGA
  AGTGTGTTCATCCCAGGAAAACCAGCCCTTGCTGAGGACGTTCGATCACCCAAATGAACGGATAATGATG
  TACGGTGGTGGCGCCCCACCTTCTTCTGCCTTTCATGACTCTGTGGACAGCATCAGCCATGCAAAAGTGG
  CCATCAAAGAGACAGTGAGGACAAACTTCCCCAGGACATGGATATGGAACCTTGTCAGTGTAGATTCCTC
  AGGCACAGCAAATGTCTCGTTCCTGGTCCCTGATACCATCACCCAGTGGGAGGCCAGCGCCTTCTGTGTC
  AATGGCAATGCTGGGTTTGGCATTTCACCCAAAGTGTCCCTACAGATCTCCCAGCCCTTCTTCGTGGAAG
  TTACCTCACCCTTTTCCGTTGTTCGAAGTGAACAGAGTGACATGGTTGTCACTGTCTTCAACTACCTGAC
  TACATGTGTAGAGATTTCTGTTCAGTTAGAAGCATCTGAGAATTATGAAGCAAGCATCAACACCCAGCGT
  AACACCGACAGTGAAGTCCTCCAGGCTGGAGAACAGAAAACATATGTCTGGACCATTATTCCTAAGACAC
  TGGGTAAAGTAAATGTCACTGTAGTGGCTACATCCAAACAGAGCAGGGCTTGCCCAAATGACGCCAGTAA
  GGAGCAAGATGTCCACTGGAAAGACACTGTCGTCAAAACCATGCTGGTAGAGGCTGAAGGTATTGAAAAA
  GAAGCAACTCAAAGTTTCCTCATCTGTCCAAAAGGTACCAAAGCCTCAAAACAAACACTTCTGGAATTGC
  CAAGCAATGTAGTAGAAGGTTCAGTCAGATCTTTTGTCACCATTGTGGGGGACATTCTGGGAGTCGCAAT
  GCAGAATCTGGAGAGCCTTCTCCAAATGCCCTATGGGTGTGGTGAACAGAACATTGCCCAGCTAGCATCT
  GACGTTTACATACTGGACTACCTGAAAGCTACCGACCAGCTGACAGAAGAACTCAAATCTAAGGCTCAGC
  GTCTCTTATCCAATGGTTACCAAAATCACTTGTCTTTCAAAAACTATGATGGGTCCTACGATGTGTTCTG
  CCAGAGCAACCAGGAAGGGAGCACATGGCTCAGTGCCCTTTCTTTCAAGACAGTTGAGAAAATGAAGGAA
  TATATTTTCATCGAAGAGACAGTCCCCAAACAGACGTTAATCTGGCTTGTAAAGAAACAGAAATCAAATG
  GATGCTTTAGAAGGGACGAGAAGCATGTCGACACGGCTCAGGAGGGCCGTGAGGGTGACCAGGAAGATAT
  TGCGCTCACTGCGTACGTCGTCGGGGTGTTCCTTGAAGTTGGACTCAATGCCAGCTTTCCTGCCCTCCGC
  AATGGTCTCTATTGCCTGGAAGAGGCATTTTCGAATGGTGTCACCAATGGCTACACGCAAGCTATTCTGG
  CTTATGTATTTGCATTAGCTGGGAAAGAACAGCAAGCGAAATCCTTACTCTCAATCCTGGACAAATCTGC
  TACAAAAACAAATAATATGATATACTGGGAGAGAGATGAGAAACCCGAGACAGACAACTCTCCATCATTC
  ATTCCTTCGGCACTTTCTGGTGAGACAGAGAAGACGTGCTATGTGCTGCTGGCTGTCCTTTCCCAGGACA
  CTCAGGACCTTGATTATGCCAGCAAGATTGTGCAGTGGCTTGCCCAGCGGATGAACTCCCATGGAGGCTT
  CTCTGCCATGCAGGACACCACAGTGTGCCTTCTCGCTCTAACCCAATACATGAAGTTAACCGGCTCTAAC
  CCTCAAAACACCATCACGCTGAGCTCAGAGGAGTCTGAGGAGGTTTTCTATGTTAACAGAAATAAACGCC
  TTCTGGTCCAGCACTCAAAAGTATCCAAAGGCCACCAACAATACACAGTGGATGTAGAAGGAGATGGATG
  CTCATTTATCCAGGCCACCCTCAGGTACAACGTGCCACTTCCTAAGGAGGCCTCAGGATTTTCCCTTTCC
  GTGAAAACTGGAAAAAGCAACTCTTCAGATGAATTCCAGACCAAGTTTGAACTCACGGTGACCCTCACCT
  ACACTGGAGCTCGTGAAAGCTCAGTTACAGTCCTTGTGGATGTGAAGATGCTCTCTGGCTTTACTCCTGT
  CGTCTCATCCACCGAGGAGCTTAAATTCAATAGTCAAGTAACGAAGACGGACATAAAGAATGGCCACGTT
  CTGTTCTACTTGGAAAATGTTCCCAAGGAAGCAACCAGCCTCACCTTCTCCATTGAACAAACTAACCATG
  TGGCCAATATCCAGCCAGCCCCAGTCACGGTCTACAGCTATGAAAAAGGTGAATATGCTTTTGATTCTTA
  CAACATCAATAGCATTTCGGATTCCCAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR218078 representing NM_001001179
  Red=Cloning site Green=Tags(s)
  
  MVPTILLSALLLHFTDVVAAEPRYILWVSSVVQRFSSEKACLHLLNLNESVSLSVTLEYDGSSTTIFDQP
  VDEGNFYACADFKVSQMSSEQLAFVALLVQGNTLKISERRSVAIAAEENATFVQTDTPVHKPGDTVHFRV
  VTLNIWLKPVDDLYPLITVQDPQSNVIFQWINVTTFRNITQLSFQLTPEPILGDYTIVIKTQSGTTVMDH
  FTVNRDVLPKFEVELTAPETITIADSQFQMVTCAKYTYGQPVQGKAQIKVCRELFSPAHCESNENEICEQ
  FTVQLKDGCASHIINTKVFQLDRSGLFMTLNVNEVVTESGTGVQMSKTHSVFITSVLGTVSFENMDPFYR
  RGITYFGTLKFSGPNNTPLVDKLLQLELDGKPVGNYTTDENGEARFSINTSEIFGAQISLKAVYVRPRSC
  HRSSWLSPEYLDAYFSASRFYSQTSSFTKIILEPKQLPCDQEKMFSVLYSLNPEAYKEASDVTFFYLVMV
  RGGISRSGQKQVRVQAWNGNFSFPISINADLAPSADLFVYTLHPSGEIVADNVRLQIEKCFKNKVSINFS
  RDKDLPGSNTSVHLQAAPDSFCALRAVDKSALLLNHGQEMTPESVYFTLPYIHQYGYFYNGLNLDDQQAE
  PCIPQKDLFYNGLYYTPTGNIWDGDLSNLLSNMGLKIFTNLHYRKPEVCSSQENQPLLRTFDHPNERIMM
  YGGGAPPSSAFHDSVDSISHAKVAIKETVRTNFPRTWIWNLVSVDSSGTANVSFLVPDTITQWEASAFCV
  NGNAGFGISPKVSLQISQPFFVEVTSPFSVVRSEQSDMVVTVFNYLTTCVEISVQLEASENYEASINTQR
  NTDSEVLQAGEQKTYVWTIIPKTLGKVNVTVVATSKQSRACPNDASKEQDVHWKDTVVKTMLVEAEGIEK
  EATQSFLICPKGTKASKQTLLELPSNVVEGSVRSFVTIVGDILGVAMQNLESLLQMPYGCGEQNIAQLAS
  DVYILDYLKATDQLTEELKSKAQRLLSNGYQNHLSFKNYDGSYDVFCQSNQEGSTWLSALSFKTVEKMKE
  YIFIEETVPKQTLIWLVKKQKSNGCFRRDEKHVDTAQEGREGDQEDIALTAYVVGVFLEVGLNASFPALR
  NGLYCLEEAFSNGVTNGYTQAILAYVFALAGKEQQAKSLLSILDKSATKTNNMIYWERDEKPETDNSPSF
  IPSALSGETEKTCYVLLAVLSQDTQDLDYASKIVQWLAQRMNSHGGFSAMQDTTVCLLALTQYMKLTGSN
  PQNTITLSSEESEEVFYVNRNKRLLVQHSKVSKGHQQYTVDVEGDGCSFIQATLRYNVPLPKEASGFSLS
  VKTGKSNSSDEFQTKFELTVTLTYTGARESSVTVLVDVKMLSGFTPVVSSTEELKFNSQVTKTDIKNGHV
  LFYLENVPKEATSLTFSIEQTNHVANIQPAPVTVYSYEKGEYAFDSYNINSISDSQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001001179
• ORF Size: 4368 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001001179.3, NP_001001179.2
• RefSeq Size: 4698 bp
• RefSeq ORF: 4371 bp
• Locus ID: 232400
• UniProt ID: Q3UU35
• Cytogenetics: 6 F3
• MW: 162.3 kDa
• Gene Summary: Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism.[UniProtKB/Swiss-Prot Function]