Ccnc (NM_001122982) Mouse Tagged ORF Clone

CAT#: MR218028

Ccnc (Myc-DDK-tagged) - Mouse cyclin C (Ccnc), transcript variant 2

Product Images

Circular map for MR218028

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ccnc
• Synonyms: AI451004; AU020987; CG1C
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR218028 representing NM_001122982
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGTGTTTCTGGCATCCAAAGTAGAGGAATTTGGTGTTGTCTCAAATACAAGATTGATTGCTGCTACT
  ACTTCTTAAAAACTAGATTTTCATATGCTTTTCCAAAGGAATTTCCTTACAGGATGAATCATATACTAGA
  ATGTGAATTTTACCTCTTAGAATTAATGGACTGTTGCTTGATAGTGTATCATCCTTATAGACCTTTGCTC
  CAGTATGTGCAGGACATGGGCCAGGAAGACGTGCTGCTTCCCCTTGCATGGAGGATAGTGAATGATACCT
  ACAGGACGGATCTCTGTCTGCTGTACCCTCCGTTCATGATCGCTTTAGCTTGCCTACATGTAGCCTGTGT
  CGTACAACAGAAAGATGCTAGACAGTGGTTTGCAGAACTTTCTGTGGATATGGAGAAGATTTTGGAAATA
  ATCAGGGTTATTTTAAAACTGTATGAGCAGTGGAAGAATTTTGATGAGAGAAAAGAGATGGCAACTATTC
  TTAGTAAGATGCCGAAACCAAAACCACCTCCAAACAGAAGCTCCCTGAGTGACTCTCCACTAATGGCAGG
  GCCTGAAGCTGCAAGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR218028 representing NM_001122982
  Red=Cloning site Green=Tags(s)
  
  MCVSGIQSRGIWCCLKYKIDCCYYFLKTRFSYAFPKEFPYRMNHILECEFYLLELMDCCLIVYHPYRPLL
  QYVQDMGQEDVLLPLAWRIVNDTYRTDLCLLYPPFMIALACLHVACVVQQKDARQWFAELSVDMEKILEI
  IRVILKLYEQWKNFDERKEMATILSKMPKPKPPPNRSSLSDSPLMAGPEAAR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001122982
• ORF Size: 576 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq Size: 1471 bp
• RefSeq ORF: 849 bp
• Locus ID: 51813
• Cytogenetics: 4 A3
• MW: 33.6 kDa
• Gene Summary: Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Binds to and activates cyclin-dependent kinase CDK8 that phosphorylates the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex (By similarity).[UniProtKB/Swiss-Prot Function]