Rusc2 (NM_199057) Mouse Tagged ORF Clone

CAT#: MR217794

Rusc2 (Myc-DDK-tagged) - Mouse RUN and SH3 domain containing 2 (Rusc2), transcript variant 1

Product Images

Circular map for MR217794

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Rusc2
• Synonyms: AI840675; mKIAA0375
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR217794 representing NM_199057
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCTTGTTGGAACTTTCCAGAATGGATAGCCCCCCTAAGCTGACTGGAGAGACCCTCATCGTCCACC
  ATATCCCCTTAGTGCACTGCCAAGTCCCAGACAGGCAGTGCTGCGGGGGCGCAGGCGGAGGCGGTGGGGG
  CACAAGAGCCAATCCCTTCTGCCCCCCGGAGCTGGGCCTCACCCAGCCTGACCACGACCTAGGACAAGCT
  GACTCCCTTCTGTACCCCAGCCTGCACTCAGCGCCAGGGGCACCTACAGGGTCTTCAGACAGCGTCAAAA
  GTAGGAGTCGGGATGGACGAGGCCCCGGAGCTCCCAAGCGCCATAATCCCTTCTTGGTGCAGGAAGGTGT
  GGGTGAGACAGGACTCGGTGACCTGCATGACAGCAGCACTGGTGACAGTGTCACCCAGCAGTCCTTCCAC
  CTGCACAGCGCCAGCCAGCCCTTCCATCTGTCTTCTTTCCAGCTGCCACCGTCCGGCCCCGGGCAGGGCA
  GGCCGTGGGGTGCAACTCACAGTCGACCTGGAGTGGTGGAGGGGCAGGAACAGGACCCAGCGACAGCCTT
  GGGGACCCAGTGCAGCACCAGCCACTGCTGCCGGCCAGAGCTGGAAGCTGAGAGGATGGAGCTGGACGAG
  TGTGGGGGACACGGGGGGAGTGGCAGTGGAGGGGGGACCAGTGATATCTCTGGCTTTTCCTTTGAGCAAG
  AGTGGAAGATCAGTTCCGACGAATCCCCAAGACACCCTGGACGCTCCGGCTCGGGAACCCAGCACTGCCA
  CTGCAGTAGTACGTCCAGTCAGTCGGAGGCGGCTGACCAGTCCATGGGCTATGTCAGCGACTCCTCCTGT
  AACAGCTCCGACGGTGTGCTAGTCACCTTCAGCACCCTCTACAATAAGATGCACAGCAGCTCCCGTGCCA
  ACCTCAACTCTGTCCCTCAGTCCTGCAGCGACTCTTCCTTCTGCAGCCACGCAGACCCTGGAGCCTTCTA
  CCTGGACCTGCAGCCCTCCCCGGCTGAGTCGAGAATGTCTTGCGAGTCCCACCACCCTGAGAACGGGGAC
  AGGGAAGAGGGCTGTGGCTGTCCTCATGTCTCATCCCCTGAGCTCGATGCCAACTGCAACGCCTACCACC
  CACACTCTGAGCCCTGCCCAGCTGTGGCTGACCTCACAGCCTGCTTCCAGAGCCAGGCCCGCCTTGTTGT
  GGCCACACAGAATTACTATAAACTTGTCACCTGTGACCTGTCCTCACAGTCCTCCCCGAGCCCCGCCGGC
  TCCTCCATCACGAGTTGCTCTGAAGAACACACCAAGATAAGTCCTCCACCAGGCCCCTGCCCAGACCCAG
  ACCCCAACCAGCCCTCTGAGTATTACCTCTTCCAGAAGCCAGACATCCAGCCAGAGGAACAAGAAGCAGT
  GGGTTCCCCAGCAGAAGCAGCAACCGCCATGGGCCCCACTGTACTTGAAGGGCAGGTGTACACGAATACT
  TCCCCCCCCAACCTCAACACTGGCCGGCAGCGCTCTCGAAGCTATGACCGTAGCCTGGAGCGCAGCCCCC
  CTGTCCGCCTGGGCTCACTAGAGCGCATGCTTAGTTGCCCAGTACGATTAAGTGAGGGCCCTGCGGCCCT
  AGCTGGGCCTGCGTCCCCACCCAGAAGGGTTACCTCCTTCGCTGAGTTGGCCAAAGGCCGGAAGAAAGCT
  GCGGGCTCCGGCTCCCCACCACTGCGGGCGAGCGTTGGAGATTCTTCCCAAGAGTTCTCGCCCATCCAAG
  AAGCCCAGCAAGACAGGGCAGCCCCACTTGACGAGGGCACTCGATGTAGCCATAGCCTGCCGTCCCTGCC
  CTTGGGGCCAAGCTTGGACCTCCTTGGCCCCGAACCCTGGTCCACCCCGGTTTGTCAGGGCTCCCAGTCC
  AGCGAGATGCCACTTCCTAGCCTCAGAGCTGCTGGGCAGGGACCCTTGGCCCAGCTGATGGATCCTGGGC
  CTGCTTTCTCGGGGAGCCCAGCCACCAGCCATACCCAGAGGGATTCGAGAGCTAGAGCTGACGGGGGTGG
  CACCGAAAGCCGACCCGTCCTTCGCTACAGCAAGGAACAGAGGCCGACCACCCTACCGATCCAGCCGTTC
  GTGTTCCAACATCACTTCCCCAAGCAGCTGGCCAAGGCCCGGGCCCTGCACAGCCTCTCCCAGCTCTACA
  GCCTCTCTATGCCCTGCAGCCGCGCACAGCAGCCCGCCCCGCTGGTTATCTCTACTGCTCAAGGCCCAGC
  CCCAGCTCCCTCAGGAGAGCCGCAGCCATTCACATCGCAGGCCTCTGGCAGAGGCGCCAGAAACGCTGGG
  CCTGAACCAGAGACCTCGCGGCCATCGCCCCTGGGAAGCTACTCCCCAGTCCGGAGTGCCGGCCCCTTTG
  GGTCTAGCACCGACTCCTCTGCCTCTACCTCCTGCTCCCCGCCTCCAGAGCAGGGCACAGCAGCCGACAG
  TGTATCACCATGGAGCCATACCTGCCCTCCTACTGTGCGCCCTGCCACATCCCAGCAGCCACCGAAGGAG
  GACCAGAAGATTCCGACCTTGGCTGAGTACAGGCTTCATGGCACAGGAAGTTTACCCCCTCTGGGCTCCT
  GGAGATCTGGCTTCACCCGGGCAGAGAGTCTGGTTCGAGGAGGTGGCGAGGGCAGCATGGCCAACAGGCC
  CAACAATGCCAACCACCTGTCCCCTCAGGCCCTCAAGTGGCGGGAATACAGGAGGAAGAACCCATTAGGG
  CCACCTGGTTTGTCAGGAAGCCTAGATCGAAGGCCACCGGAAGCTCGGCTGGCCCGCAGGAACCCCATCT
  TTGAGTTCCCTGGTTCTTTCGGCACAACCAGCCATCTGAATTGCCGGCTGAATGGTCAGATTTCAAAGCC
  ACTGTCGCTGACCTGTCCTGACCTGCAGGACCCCTTCTCCTTAACCGAGAAGCCTCCCGCTGAGTTCTGT
  CTGTCTCCCGATGGCAACTCGGAGGCCATATCCATCGACATACTTCAGAAAAAAGGGCTGGTAAAAGCTG
  TCAACACCGCTGTGGACCTTATTGTGGCCCATTTTGGCACGAGCCGGGATCCTGGCGTGAAGGCAAAGCT
  GGGCAACAGTTCTGTGAGCCCCAATGTCGGCCACCTGGTTCTGAAGTACTTGTGCCCTGCAGTCCAGGCC
  GTGCTCGAGGACGGACTCAAGGCCTTCGTACTGGATGTGATCATCGGCCAACGTAAAAACATGCCTTGGA
  GTGTGGTCGAGGCTTCCACACAGCTAGGCCCATCCACCAAGGTCTTGCATGGCCTCTACAACAAAGTCAG
  CCAGTTCCCAGAACTCACCAGTCACACCATGCGCTTCAACGCCTTCATCCTCGGCCTGCTCAACATCCGG
  TCTCTGGAGTTCTGGTTTAATCACCTGTATAACCATGAAGATATCATCCAGACCCACTACCAGCCCTGGG
  GCTTCCTGCGGGCCGCACACACGGTGTGCCCAGGCCTCTTCGAGGAGCTGCTGCTGTTGCTACAGCCCTT
  AGCCCTGCTACCCTTCAGCCTGGACTTGCTGTTCCAGCACCGGCTGCTGCAGAGCGGGCGGCAGCAGCGG
  CAGCACAAGGAACTGCTGCGTGTGTCCCAGGACCTGCTGCTGTCTGCGCACTCAACACTGCAGTTGGCCA
  GGTCCCGAGGCCAGGAGGGCCCTGGAGACATGGACAGGGTGGCTCCTGGGGAACGGGTGAAGGGGGTGGG
  TGCCTCAGAAGGTGGAGAAGAGGACGAGGAAGACGCGGAAGAGGTGGCAGTGGTGGCGGGGAGCTCGGAT
  CATGGCAAGTGGGCCCGAGGGGGTCAGGCTGGCTGGTGGTACCAGCTCATGCAGAGCTCCCAGGTCTACA
  TCGATGGCACCGCTGAGGGCTCTAGATTCCCTCGCAGCAGCAGCAGCAGCAGTGGCAGCGGCAGTGAGAA
  GAAGAAAGGAGTAGGCAGTGGGGGGCCGTCCCAGGCTCCGCCTCCGCCGCCTCGGGAAGGTGTGGTAGAG
  GGGGCGGAGGCCTGCCCTGCCCCTGAGGAGGCCCTTGGCCAAGAAAGGGGCTGGCCTTTCTGGATGGGGA
  GCCCCCCTGACTCTGTGCTAGCTGAGCTGAGGCGTAGTCGAGAGAGGGAAGGGCCTGTTGCCCCACCGAC
  GGAAAATGAGGAAGGGACCGCAGAGCCTTCACCTGGGGGCATCAAGTGGGGACATCTCTTTGGTTCTCGG
  AAATCTCAGCGGGAAGCCCGGCCCACGAACAGGCTGCCGTCGGACTGGCTGAGCCTGGACAAGTCTGTGT
  TCCAGCTGGTAGCACAGACGATGGGGGCCCGTCGGGAGCCCGAGCCCAGGGAGAACCTGCAAGAGTCACA
  CCCTCCAGCCGTGCCCTCCAAACCTCCGTGCGAGGTGCAGGCTCTGTGCCACCATCTGGCCACAGGCCCT
  GGACAACTGAGCTTCCACAAAGGAGATATCCTCCGGGTGCTGGGACCGGCCAGAGGAGACTGGCTGCGCT
  GCAGCCGAGGCCCTGACACCGGCTTGGTACCTCTGGCCTATGTGACATTGACCCCAACTCCAAGTTCACC
  TCCTGGGAGCAGCCAAAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR217794 representing NM_199057
  Red=Cloning site Green=Tags(s)
  
  MPLLELSRMDSPPKLTGETLIVHHIPLVHCQVPDRQCCGGAGGGGGGTRANPFCPPELGLTQPDHDLGQA
  DSLLYPSLHSAPGAPTGSSDSVKSRSRDGRGPGAPKRHNPFLVQEGVGETGLGDLHDSSTGDSVTQQSFH
  LHSASQPFHLSSFQLPPSGPGQGRPWGATHSRPGVVEGQEQDPATALGTQCSTSHCCRPELEAERMELDE
  CGGHGGSGSGGGTSDISGFSFEQEWKISSDESPRHPGRSGSGTQHCHCSSTSSQSEAADQSMGYVSDSSC
  NSSDGVLVTFSTLYNKMHSSSRANLNSVPQSCSDSSFCSHADPGAFYLDLQPSPAESRMSCESHHPENGD
  REEGCGCPHVSSPELDANCNAYHPHSEPCPAVADLTACFQSQARLVVATQNYYKLVTCDLSSQSSPSPAG
  SSITSCSEEHTKISPPPGPCPDPDPNQPSEYYLFQKPDIQPEEQEAVGSPAEAATAMGPTVLEGQVYTNT
  SPPNLNTGRQRSRSYDRSLERSPPVRLGSLERMLSCPVRLSEGPAALAGPASPPRRVTSFAELAKGRKKA
  AGSGSPPLRASVGDSSQEFSPIQEAQQDRAAPLDEGTRCSHSLPSLPLGPSLDLLGPEPWSTPVCQGSQS
  SEMPLPSLRAAGQGPLAQLMDPGPAFSGSPATSHTQRDSRARADGGGTESRPVLRYSKEQRPTTLPIQPF
  VFQHHFPKQLAKARALHSLSQLYSLSMPCSRAQQPAPLVISTAQGPAPAPSGEPQPFTSQASGRGARNAG
  PEPETSRPSPLGSYSPVRSAGPFGSSTDSSASTSCSPPPEQGTAADSVSPWSHTCPPTVRPATSQQPPKE
  DQKIPTLAEYRLHGTGSLPPLGSWRSGFTRAESLVRGGGEGSMANRPNNANHLSPQALKWREYRRKNPLG
  PPGLSGSLDRRPPEARLARRNPIFEFPGSFGTTSHLNCRLNGQISKPLSLTCPDLQDPFSLTEKPPAEFC
  LSPDGNSEAISIDILQKKGLVKAVNTAVDLIVAHFGTSRDPGVKAKLGNSSVSPNVGHLVLKYLCPAVQA
  VLEDGLKAFVLDVIIGQRKNMPWSVVEASTQLGPSTKVLHGLYNKVSQFPELTSHTMRFNAFILGLLNIR
  SLEFWFNHLYNHEDIIQTHYQPWGFLRAAHTVCPGLFEELLLLLQPLALLPFSLDLLFQHRLLQSGRQQR
  QHKELLRVSQDLLLSAHSTLQLARSRGQEGPGDMDRVAPGERVKGVGASEGGEEDEEDAEEVAVVAGSSD
  HGKWARGGQAGWWYQLMQSSQVYIDGTAEGSRFPRSSSSSSGSGSEKKKGVGSGGPSQAPPPPPREGVVE
  GAEACPAPEEALGQERGWPFWMGSPPDSVLAELRRSREREGPVAPPTENEEGTAEPSPGGIKWGHLFGSR
  KSQREARPTNRLPSDWLSLDKSVFQLVAQTMGARREPEPRENLQESHPPAVPSKPPCEVQALCHHLATGP
  GQLSFHKGDILRVLGPARGDWLRCSRGPDTGLVPLAYVTLTPTPSSPPGSSQN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_199057
• ORF Size: 4569 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_199057.3, NP_951012.2
• RefSeq Size: 5278 bp
• RefSeq ORF: 4572 bp
• Locus ID: 100213
• Cytogenetics: 4 A5
• MW: 162.3 kDa