A4galt (NM_001004150) Mouse Tagged ORF Clone

CAT#: MR217727

A4galt (Myc-DDK-tagged) - Mouse alpha 1,4-galactosyltransferase (A4galt), transcript variant 2

Product Images

Circular map for MR217727

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: A4galt
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR217727 representing NM_001004150
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCATCTCCTGTTCCCATCTGGAGGAGACCATGTCCAAGCCCCCCGACTGCCTGCTGCGGATGCTCA
  GGGGCACCCCCAGACAGCGGGTCTTCACCTTCTTCATCATCAGCTTCAAGTTCATGTTCTTGATCTCCAT
  CCTGATCTACTGGCACACTGTGGGTGCACCCAAGGACCAAAGAGAGTATAGCCTGCCAGTAGACTTCTCC
  TGCCCCCAGCTGGCCTTTCCTAGAGTCTCTGCCCCAGGCAACATCTTCTTCCTAGAGACATCGGACAGGA
  CCAGTCCCAACTTTCTATTTATGTGTTCTGTGGAATCAGCTGCCAGGGCACACCCTGAATCCCAGGTGGT
  TGTGCTGATGAAAGGGCTGCCTAGGGACACCACAGCTCAGCCCCGGAACCTCGGCATCTCTCTTCTGAGC
  TGTTTTCCTAATGTCTGGATACGACCTCTGGACTTGCAAGAACTGTTTGAGGACACACCACTGGCAGCCT
  GGTACTCGGAGGCACGGCACAGATGGGAGCCCTACCAGTTGCCCGTACTGTCTGATGCTTCCAGGATCGC
  ACTCCTCTGGAAGTTTGGTGGGATCTACCTGGACACAGACTTCATCGTCCTCAAGAACCTGCTCAACCTG
  ACCAACACGCTGGGCATCCAATCCCGCTATGTCCTCAACGGTGCCTTCCTAGCCTTCGAGCGCAAGCATG
  AGTTCTTGGCACTGTGTCTACATGACTTCGTGGCCAACTACAATGGTTGGATTTGGGGTCACCAGGGCCC
  CCAACTGCTTACCCGAGTCTTCAAGAAGTGGTGTTCCATCCAAAGCCTGGAAAAGAGCCATGCTTGCCGT
  GGGGTCACTGCCCTGCCCCCCGAGGCCTTCTACCCCATCCCCTGGCAGAACTGGAAGAAATACTTTGAAG
  ACATCAGCCCCGAGGAGCTGACCCAGCTGCTCAATGCCACCTATGCTGTTCATGTGTGGAACAAGAAAAG
  TCAGGGTACACACCTAGAGGCCACATCCAAGGCTCTGCTGGCCCAGCTACATGCCCGCTATTGCCCTACG
  ACACATAGAGCCATGAAGATGTACTTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR217727 representing NM_001004150
  Red=Cloning site Green=Tags(s)
  
  MGISCSHLEETMSKPPDCLLRMLRGTPRQRVFTFFIISFKFMFLISILIYWHTVGAPKDQREYSLPVDFS
  CPQLAFPRVSAPGNIFFLETSDRTSPNFLFMCSVESAARAHPESQVVVLMKGLPRDTTAQPRNLGISLLS
  CFPNVWIRPLDLQELFEDTPLAAWYSEARHRWEPYQLPVLSDASRIALLWKFGGIYLDTDFIVLKNLLNL
  TNTLGIQSRYVLNGAFLAFERKHEFLALCLHDFVANYNGWIWGHQGPQLLTRVFKKWCSIQSLEKSHACR
  GVTALPPEAFYPIPWQNWKKYFEDISPEELTQLLNATYAVHVWNKKSQGTHLEATSKALLAQLHARYCPT
  THRAMKMYL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001004150
• ORF Size: 1077 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001004150.3, NP_001004150.1
• RefSeq Size: 2105 bp
• RefSeq ORF: 1047 bp
• Locus ID: 239559
• UniProt ID: Q67BJ4
• Cytogenetics: 15 E1
• MW: 41.8 kDa
• Gene Summary: The protein encoded by this gene catalyzes the transfer of galactose to lactosylceramide to form globotriaosylceramide, which has been identified as the P(k) antigen of the P blood group system. The encoded protein, which is a type II membrane protein found in the Golgi, is also required for the synthesis of the bacterial verotoxins receptor. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Jan 2010]