Spert (NM_001164140) Mouse Tagged ORF Clone

CAT#: MR217722

Spert (Myc-DDK-tagged) - Mouse spermatid associated (Spert), transcript variant 4

Product Images

Circular map for MR217722

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Spert
• Synonyms: 1700086N05Rik; AI596460; Nurit
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR217722 representing NM_001164140
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCACCTCTGGAATGTTCTGAGTGTTTTGGTGACCAGCTTTTGCATAAGACCTATACCTGGCATCTCA
  CATTGAGGGGCACCGAGCCCTTCCTGAGGCTCCACAACCTGTACAGTACTCCTCGCTGCTCCAGGCAGGC
  TGCCTTACCTCGGATGAGCCGCAGGGTAGCCAGCCAGCACTCCTACCCACTCAACCGCTTCTCTTCTATG
  CCTTTTGACCCCATGGAGCGCCCCACCTCCCAGGCCGACCTGGAACTAGACTACAACCCTCCCCGGGTGC
  AGCTCAGCGATGAGATGTTTGTCTTCCAGGATGGGCGCTGGGTGAATGAGAGCTGCCGCCTACAACCACC
  TTACTTCTCTCCCCCCTCCTCCTTCCACCATAAGCTGCACCACAGGAGGCTGGCCAAGGAGTACCAGCTG
  CAGGAAGAGAACAAGTCTCTTCGGGATGAGAACCGGGCCCTACGGGATGAGAACAAGGCCCTCCGCAAGG
  AGAACAAGATCTTACAGGTCTTTTGGGAAGAGCACAAGGTCACTTTAGGCCACGAGGAGAGTCAGACCTC
  CTCATTGTTGCACAAGGACACAACCTCCCAGGAAGTGGTGAAGAGAGACAACACCACTTTGCCTGCCCAG
  CGTAGCAAAGAGAGCACCCTACAGCTCATTCGAGAGGAGAACCGGGCCCTGCAGCAGTTGCTGGAGCAGA
  GGCAGGCCTACTGGGCCCAGGCAGAAGAGAATGCAGCTTCCACTGAGGAAGGCAAGTCCACCTCCTCACC
  CAAGGAGGAGTCCCATAACTCAGGGCTTCTGCCAGATCAGAGCACAAACCACTCCTCCCCCTTTGAGGAC
  CCCAAAGTGCCCCCCACAACACAGGAGGACTCGAAGACACTGCGTGCCCTGAGGGAGATGGTTAACAACT
  TGTCTGGGGCATCTGGGGAAGAGGATGGCAAAGTAGGCCCTAACCTACCTGACAGCGCTCAACCCCTGCA
  GCTGCTGAGAGAGATGAACCAGGCGCTGCAGGCCCTGCAGGAGGAGAACCGGCTGCTGCAGGAGGAGAAC
  AGGGCTCTGCACATCTTGCGAGAGGAGCACCGCGTCTTCCAGGAGGAGAACAAGGCCCTGTGGGAGAACA
  ATAAGTTGAAGCTGCAGCAGCGGCTGGTCATCGACACGGTGACTGAGGTCACAGCCCGGATGGAGATGCT
  TATCGAAGAGCTGTATGCCTTCATGCCAGCCAAGAACAACAAAGATCCCAAAAAGCCCAGCAGGGTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR217722 representing NM_001164140
  Red=Cloning site Green=Tags(s)
  
  MSPLECSECFGDQLLHKTYTWHLTLRGTEPFLRLHNLYSTPRCSRQAALPRMSRRVASQHSYPLNRFSSM
  PFDPMERPTSQADLELDYNPPRVQLSDEMFVFQDGRWVNESCRLQPPYFSPPSSFHHKLHHRRLAKEYQL
  QEENKSLRDENRALRDENKALRKENKILQVFWEEHKVTLGHEESQTSSLLHKDTTSQEVVKRDNTTLPAQ
  RSKESTLQLIREENRALQQLLEQRQAYWAQAEENAASTEEGKSTSSPKEESHNSGLLPDQSTNHSSPFED
  PKVPPTTQEDSKTLRALREMVNNLSGASGEEDGKVGPNLPDSAQPLQLLREMNQALQALQEENRLLQEEN
  RALHILREEHRVFQEENKALWENNKLKLQQRLVIDTVTEVTARMEMLIEELYAFMPAKNNKDPKKPSRV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001164140
• ORF Size: 1257 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001164140.1, NP_001157612.1
• RefSeq Size: 1534 bp
• RefSeq ORF: 1260 bp
• Locus ID: 67926
• Cytogenetics: 14 D3
• MW: 49.1 kDa