Syngr1 (NM_009303) Mouse Tagged ORF Clone

CAT#: MR217607

Syngr1 (Myc-DDK-tagged) - Mouse synaptogyrin 1 (Syngr1), transcript variant 1b

Product Images

Circular map for MR217607

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Syngr1
• Synonyms: p29; R75468; Syngr1b
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR217607 representing NM_009303
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGGGGGTGCGTACGGAGCGGGCAAAGCCGGGGGCGCCTTCGACCCCTACACCCTGGTGAGGCAGC
  CACACACCATCCTGCGCGTCGTGTCTTGGGTCTTCTCCATCGTGGTCTTCGGCTCTATCGTGAACGAGGG
  CTACCTCAACAACCCAGAGGAGGAGGAGGAGTTCTGCATCTACAACCGCAACCCCAATGCCTGCAGCTAC
  GGTGTGACTGTGGGTGTGTTGGCCTTCCTCACCTGCCTGCTCTACCTGGCGCTCGATGTGTACTTCCCGC
  AGATCAGCAGTGTCAAGGACCGCAAGAAGGCCGTGCTCTCTGACATCGGTGTCTCGGCCTTCTGGGCCTT
  CTTCTGGTTCGTGGGTTTCTGCTTCCTGGCCAACCAGTGGCAAGTCTCCAAGCCCAAGGACAACCCTCTG
  AACGAAGGGACGGACGCAGCCCGGGCTGCCATCGCCTTCTCCTTCTTCTCCATTTTCACGTGGAGCCTGA
  CAGCAGCCCTGGCCGTACGCAGATTCAAGGAGCTTACCTTCCAGGAGGAATACAACACACTGTTCCCTGC
  CTCCGCACAACCG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR217607 representing NM_009303
  Red=Cloning site Green=Tags(s)
  
  MEGGAYGAGKAGGAFDPYTLVRQPHTILRVVSWVFSIVVFGSIVNEGYLNNPEEEEEFCIYNRNPNACSY
  GVTVGVLAFLTCLLYLALDVYFPQISSVKDRKKAVLSDIGVSAFWAFFWFVGFCFLANQWQVSKPKDNPL
  NEGTDAARAAIAFSFFSIFTWSLTAALAVRRFKELTFQEEYNTLFPASAQP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009303
• ORF Size: 573 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009303.2, NP_033329.1
• RefSeq Size: 818 bp
• RefSeq ORF: 576 bp
• Locus ID: 20972
• UniProt ID: O55100
• Cytogenetics: 15 E1
• MW: 21.7 kDa
• Gene Summary: May play a role in regulated exocytosis. Modulates the localization of synaptophysin/SYP into synaptic-like microvesicles and may therefore play a role in synaptic-like microvesicle formation and/or maturation (By similarity). Involved in the regulation of short-term and long-term synaptic plasticity (PubMed:10595519).[UniProtKB/Swiss-Prot Function]