Ankib1 (NM_001003909) Mouse Tagged ORF Clone

CAT#: MR217460

Ankib1 (Myc-DDK-tagged) - Mouse ankyrin repeat and IBR domain containing 1 (Ankib1)

Product Images

Circular map for MR217460

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ankib1
• Synonyms: 2310061P20Rik; 4631416I11Rik; AW494740; C80642; mKIAA1386
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR217460 representing NM_001003909
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAAATACAACCACCAAATTCCGCAAAGCACTCATCAATGGTGATGAAAATTTGGCCTGCCAAATAT
  ATGAAAATAATCCTCAGCTAAAAGAATCCCTTGATCCCAATATTTCTTATGGAGAACCCTATCAGCACAA
  CACACCGTTACACTATGCTGCTAGACATGGAATGAATAGAATATTAGGGACTTTTCTTTTTGGTAGAGAT
  GGAAACCCAAATAAACGGAATGTGCACAATGAAACATCTATGCATTTGTTGTGTATGGGACCTCAAATCA
  TGATATCTGAAGGAACCCTTCATCCTCGCTTAGCACGGCCTGTGGAAGATGATTTCAGAAGGGCAGATTG
  TCTGCAGATGATCTTACAGTGGAAAGGAGCAAAACTTGACCAAGGCGAATATGAGAGAGCGGCTATTGAT
  GCCGTTGATAACAAAAAGAACACACCCCTTCACTATGCTGCAGCCTCGGGGATGAAAGCCTGTGTAGAGC
  TCTTAGTAAAACATGGAGGAGACTTGTTTGCTGAAAATGAAAATAGAGATACTCCTTGTGATTGTGCCGA
  AAAGCAGCAGCACAAAGACCTGGCCCTCAGTCTGGAGTCTCAGATGGTGTTCTCTCGGGACCCGGAGGCC
  GAAGAAATAGAAGCGGAGTATGCTGCCTTAGACAAGCGAGAGCCATATGAAGGACTAAGGCCCCAGGATC
  TTCGTAGGTTAAAAGATATGCTTATTGTGGAGACTGCAGACATGCTCCAGGCCCCTCTGTTTACTGCTGA
  AGCACTGCTTCGAGCTCACGATTGGGACAGGGAGAAGTTGCTTGAAGCTTGGATGTCCAACCCAGAGAAC
  TGCTGCCAGCGGTCAGGGGTGCAGATGCCAACCCCACCACCCAGTGGGTACAATGCCTGGGACACGCTGC
  CTTCCCCGAGAACTCCAAGGACTACAAGATCCTCTGTCACCTCCCCAGATGAAATCAGTCTGTCTCCTGG
  GGATTTAGATACCAGTTTGTGTGACATTTGTATGTGCAGTATTTCTGTGTTTGAAGATCCAGTGGATATG
  CCCTGTGGACATGATTTTTGTAGAGGCTGTTGGGAGGCGTTTTTAAATCTGAAAATTCAAGAAGGTGAAG
  CCCATAACATTTTCTGCCCTGCATATGAATGCTTTCAACTCGTGCCTGTAGATGTCATAGAAAGTGTAGT
  TTCTAAGGAGATGGACAAACGATACCTACAGTTTGATATTAAGGCCTTTGTTGAAAATAATCCTGCCATT
  AAATGGTGTCCTACCGCAGGCTGTGAACGAGCAGTCAGACTGACAAAGCAGGGGTCAAATCCATCTGGGT
  CTGACACACTCAGCTTCCCATTGCTAAGAGCCCCTGCTGTGGACTGCGGCAAAGGACACCTCTTCTGCTG
  GGAGTGCCTTGGTGAAGCACATGAGCCTTGTGACTGCCAAACATGGAAAAACTGGTTACAAAAGATAACC
  GAAATGAAGCCAGAAGAACTCGTTGGAGTCAGCGAAGCCTATGAAGATGCTGCCAATTGTCTGTGGCTGT
  TAACTAACTCCAAGCCTTGTGCGAACTGTAAGTCTCCAATACAGAAGAACGAAGGCTGTAATCACATGCA
  GTGTGCCAAGTGCAAGTATGACTTTTGCTGGATTTGCCTAGAAGAATGGAAAAAACATAGTTCTTCCACT
  GGAGGTTACTACAGATGCACTCGCTATGAAGTCATCCAACATGTGGAGGAGCAATCCAAGGAGATGACAG
  TGGAGGCTGAGAAGAAACATAAACGGTTTCAGGAACTTGACAGATTTATGCACTATTATACAAGATATAA
  AAACCATGAGCATAGTTATCAGTTAGAACAACGCCTTCTTAAAACAGCCAAAGAAAAGATGGAGCAGTTG
  AGTAGAGCTCTCAAAGAAACTGAAGGAGGCTGTCCAGATACCACTTTCATCGAAGATGCAGTGCATGTTC
  TTCTAAAAACTCGGCGCATCCTCAAGTGTTCTTATCCATATGGATTTTTCTTAGAACCTAAAAGCACAAA
  GAAAGAAATTTTTGAACTAATGCAAACAGACCTGGAAATGGTCACTGAAGACCTTGCCCAGAAAGTCAAT
  AGGCCTTATCTTCGTACACCTCGGCACAAGATCATCAGGGCAGCATGCCTTGTGCAGCAGAAAAGGCAAG
  AGTTCCTGGCATCTGTCGCTCGTGGTGTTGCTCCTGCAGACTCACCAGATGCTCCCAGGCGCAGCTTTGC
  CGGTGGAACATGGGATTGGGAATATTTAGGATTTGCTTCACCTGAGTATCGGAGGAGACACAGACAGCAG
  CGCCGTCGAGGAGATGTGCATAGCCTGCTCAGTAACCCTACAGACCTGGATGAACCAAGCGAAAGCACTT
  TCGATCTCCCAGAGGGGAGCAGCGGCCGCAGGCCTGGTGCATCTGTGGTCAGCTCTGCATCGATGAGTGT
  GCTGCACAGCTCTTCCCTGCGAGACTACAGCCCTGCCAGTCGCTCTGCAAACCAGGACTCTCTTCAGGCT
  CTGAGTTCCTTGGATGAAGATGATCCCAACATACTCCTGGCCATCCAGTTGTCACTGCAAGAGTCTGGGC
  TGGACATGGATGAAGAGACCAGAGACTTCCTTAGTAACGAGACATCCCTAGGAGCCATAGGCTCATCTTT
  ACCTTCCCGACTGGACTCTGTGCCCCGCAGCACTGAGTCTCCTCGGGCTGCGCTGAGCAGCTCGGAGCTG
  CTGGAGCTTGGTGACAGTCTCATGAGACTAGGAGCAGACAGTGACCCATTTTCAACTGACACTTTGAGTT
  CACGCCCTCTCAGTGAGACAAGAAGTGATTTCTGTCCCTCTTCCAGTGACCTTGACTCAGCTGGCCAGGA
  CCCCAGTGCCAATGACAATCTTCTTGGCAATATTATGGCTTGGTTTCATGACATGAACCCGCAGAGTATC
  GCCCTGATTCCTCCAGCAGCAACCACAGAAATTAGTGCTGAGCCTCAGCTTCCTTGTATAAGAGATGGGT
  CTGAAGGTGTGAGGGACATGGAACTCGTGCCACCAGAAGACTCAGTGTCCAAAGATACTGGTGTTCATGA
  AGGTGAAAGAGCCCAGATGGAAGAAAATCCTCTGGAAGAGAATATTCTGGCCAGGGAAGAGTTGTCTCAA
  GCTGGTGACAGTAGTAACGAGGCAGTGGGCAGGGGGGACAGGCCAGATGCTGCCAGTCAAACCCCTCAGA
  CCTCAAGCGACTGGCTCGAGCAAGTACATTCAGTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR217460 representing NM_001003909
  Red=Cloning site Green=Tags(s)
  
  MGNTTTKFRKALINGDENLACQIYENNPQLKESLDPNISYGEPYQHNTPLHYAARHGMNRILGTFLFGRD
  GNPNKRNVHNETSMHLLCMGPQIMISEGTLHPRLARPVEDDFRRADCLQMILQWKGAKLDQGEYERAAID
  AVDNKKNTPLHYAAASGMKACVELLVKHGGDLFAENENRDTPCDCAEKQQHKDLALSLESQMVFSRDPEA
  EEIEAEYAALDKREPYEGLRPQDLRRLKDMLIVETADMLQAPLFTAEALLRAHDWDREKLLEAWMSNPEN
  CCQRSGVQMPTPPPSGYNAWDTLPSPRTPRTTRSSVTSPDEISLSPGDLDTSLCDICMCSISVFEDPVDM
  PCGHDFCRGCWEAFLNLKIQEGEAHNIFCPAYECFQLVPVDVIESVVSKEMDKRYLQFDIKAFVENNPAI
  KWCPTAGCERAVRLTKQGSNPSGSDTLSFPLLRAPAVDCGKGHLFCWECLGEAHEPCDCQTWKNWLQKIT
  EMKPEELVGVSEAYEDAANCLWLLTNSKPCANCKSPIQKNEGCNHMQCAKCKYDFCWICLEEWKKHSSST
  GGYYRCTRYEVIQHVEEQSKEMTVEAEKKHKRFQELDRFMHYYTRYKNHEHSYQLEQRLLKTAKEKMEQL
  SRALKETEGGCPDTTFIEDAVHVLLKTRRILKCSYPYGFFLEPKSTKKEIFELMQTDLEMVTEDLAQKVN
  RPYLRTPRHKIIRAACLVQQKRQEFLASVARGVAPADSPDAPRRSFAGGTWDWEYLGFASPEYRRRHRQQ
  RRRGDVHSLLSNPTDLDEPSESTFDLPEGSSGRRPGASVVSSASMSVLHSSSLRDYSPASRSANQDSLQA
  LSSLDEDDPNILLAIQLSLQESGLDMDEETRDFLSNETSLGAIGSSLPSRLDSVPRSTESPRAALSSSEL
  LELGDSLMRLGADSDPFSTDTLSSRPLSETRSDFCPSSSDLDSAGQDPSANDNLLGNIMAWFHDMNPQSI
  ALIPPAATTEISAEPQLPCIRDGSEGVRDMELVPPEDSVSKDTGVHEGERAQMEENPLEENILAREELSQ
  AGDSSNEAVGRGDRPDAASQTPQTSSDWLEQVHSV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001003909
• ORF Size: 3255 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001003909.4, NP_001003909.2
• RefSeq Size: 6626 bp
• RefSeq ORF: 3258 bp
• Locus ID: 70797
• UniProt ID: Q6ZPS6
• Cytogenetics: 5 A1
• MW: 121.9 kDa
• Gene Summary: Might act as an E3 ubiquitin-protein ligase, or as part of E3 complex, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes and then transfers it to substrates.[UniProtKB/Swiss-Prot Function]