Ankib1 (NM_001003909) Mouse Untagged Clone

CAT#: MC223480

Ankib1 (untagged) - Mouse ankyrin repeat and IBR domain containing 1 (Ankib1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ankib1
• Synonyms: 2310061P20Rik; 4631416I11Rik; AW494740; C80642; mKIAA1386
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223480 representing NM_001003909
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAAATACAACCACCAAATTCCGCAAAGCACTCATCAATGGTGATGAAAATTTGGCCTGCCAAATAT
  ATGAAAATAATCCTCAGCTAAAAGAATCCCTTGATCCCAATATTTCTTATGGAGAACCCTATCAGCACAA
  CACACCGTTACACTATGCTGCTAGACATGGAATGAATAGAATATTAGGGACTTTTCTTTTTGGTAGAGAT
  GGAAACCCAAATAAACGGAATGTGCACAATGAAACATCTATGCATTTGTTGTGTATGGGACCTCAAATCA
  TGATATCTGAAGGAACCCTTCATCCTCGCTTAGCACGGCCTGTGGAAGATGATTTCAGAAGGGCAGATTG
  TCTGCAGATGATCTTACAGTGGAAAGGAGCAAAACTTGACCAAGGCGAATATGAGAGAGCGGCTATTGAT
  GCCGTTGATAACAAAAAGAACACACCCCTTCACTATGCTGCAGCCTCGGGGATGAAAGCCTGTGTAGAGC
  TCTTAGTAAAACATGGAGGAGACTTGTTTGCTGAAAATGAAAATAGAGATACTCCTTGTGATTGTGCCGA
  AAAGCAGCAGCACAAAGACCTGGCCCTCAGTCTGGAGTCTCAGATGGTGTTCTCTCGGGACCCGGAGGCC
  GAAGAAATAGAAGCGGAGTATGCTGCCTTAGACAAGCGAGAGCCATATGAAGGACTAAGGCCCCAGGATC
  TTCGTAGGTTAAAAGATATGCTTATTGTGGAGACTGCAGACATGCTCCAGGCCCCTCTGTTTACTGCTGA
  AGCACTGCTTCGAGCTCACGATTGGGACAGGGAGAAGTTGCTTGAAGCTTGGATGTCCAACCCAGAGAAC
  TGCTGCCAGCGGTCAGGGGTGCAGATGCCAACCCCACCACCCAGTGGGTACAATGCCTGGGACACGCTGC
  CTTCCCCGAGAACTCCAAGGACTACAAGATCCTCTGTCACCTCCCCAGATGAAATCAGTCTGTCTCCTGG
  GGATTTAGATACCAGTTTGTGTGACATTTGTATGTGCAGTATTTCTGTGTTTGAAGATCCAGTGGATATG
  CCCTGTGGACATGATTTTTGTAGAGGCTGTTGGGAGGCGTTTTTAAATCTGAAAATTCAAGAAGGTGAAG
  CCCATAACATTTTCTGCCCTGCATATGAATGCTTTCAACTCGTGCCTGTAGATGTCATAGAAAGTGTAGT
  TTCTAAGGAGATGGACAAACGATACCTACAGTTTGATATTAAGGCCTTTGTTGAAAATAATCCTGCCATT
  AAATGGTGTCCTACCGCAGGCTGTGAACGAGCAGTCAGACTGACAAAGCAGGGGTCAAATCCATCTGGGT
  CTGACACACTCAGCTTCCCATTGCTAAGAGCCCCTGCTGTGGACTGCGGCAAAGGACACCTCTTCTGCTG
  GGAGTGCCTTGGTGAAGCACATGAGCCTTGTGACTGCCAAACATGGAAAAACTGGTTACAAAAGATAACC
  GAAATGAAGCCAGAAGAACTCGTTGGAGTCAGCGAAGCCTATGAAGATGCTGCCAATTGTCTGTGGCTGT
  TAACTAACTCCAAGCCTTGTGCGAACTGTAAGTCTCCAATACAGAAGAACGAAGGCTGTAATCACATGCA
  GTGTGCCAAGTGCAAGTATGACTTTTGCTGGATTTGCCTAGAAGAATGGAAAAAACATAGTTCTTCCACT
  GGAGGTTACTACAGATGCACTCGCTATGAAGTCATCCAACATGTGGAGGAGCAATCCAAGGAGATGACAG
  TGGAGGCTGAGAAGAAACATAAACGGTTTCAGGAACTTGACAGATTTATGCACTATTATACAAGATATAA
  AAACCATGAGCATAGTTATCAGTTAGAACAACGCCTTCTTAAAACAGCCAAAGAAAAGATGGAGCAGTTG
  AGTAGAGCTCTCAAAGAAACTGAAGGAGGCTGTCCAGATACCACTTTCATCGAAGATGCAGTGCATGTTC
  TTCTAAAAACTCGGCGCATCCTCAAGTGTTCTTATCCATATGGATTTTTCTTAGAACCTAAAAGCACAAA
  GAAAGAAATTTTTGAACTAATGCAAACAGACCTGGAAATGGTCACTGAAGACCTTGCCCAGAAAGTCAAT
  AGGCCTTATCTTCGTACACCTCGGCACAAGATCATCAGGGCAGCATGCCTTGTGCAGCAGAAAAGGCAAG
  AGTTCCTGGCATCTGTCGCTCGTGGTGTTGCTCCTGCAGACTCACCAGATGCTCCCAGGCGCAGCTTTGC
  CGGTGGAACATGGGATTGGGAATATTTAGGATTTGCTTCACCTGAGTATCGGAGGAGACACAGACAGCAG
  CGCCGTCGAGGAGATGTGCATAGCCTGCTCAGTAACCCTACAGACCTGGATGAACCAAGCGAAAGCACTT
  TCGATCTCCCAGAGGGGAGCAGCGGCCGCAGGCCTGGTGCATCTGTGGTCAGCTCTGCATCGATGAGTGT
  GCTGCACAGCTCTTCCCTGCGAGACTACAGCCCTGCCAGTCGCTCTGCAAACCAGGACTCTCTTCAGGCT
  CTGAGTTCCTTGGATGAAGATGATCCCAACATACTCCTGGCCATCCAGTTGTCACTGCAAGAGTCTGGGC
  TGGACATGGATGAAGAGACCAGAGACTTCCTTAGTAACGAGACATCCCTAGGAGCCATAGGCTCATCTTT
  ACCTTCCCGACTGGACTCTGTGCCCCGCAGCACTGAGTCTCCTCGGGCTGCGCTGAGCAGCTCGGAGCTG
  CTGGAGCTTGGTGACAGTCTCATGAGACTAGGAGCAGACAGTGACCCATTTTCAACTGACACTTTGAGTT
  CACGCCCTCTCAGTGAGACAAGAAGTGATTTCTGTCCCTCTTCCAGTGACCTTGACTCAGCTGGCCAGGA
  CCCCAGTGCCAATGACAATCTTCTTGGCAATATTATGGCTTGGTTTCATGACATGAACCCGCAGAGTATC
  GCCCTGATTCCTCCAGCAGCAACCACAGAAATTAGTGCTGAGCCTCAGCTTCCTTGTATAAGAGATGGGT
  CTGAAGGTGTGAGGGACATGGAACTCGTGCCACCAGAAGACTCAGTGTCCAAAGATACTGGTGTTCATGA
  AGGTGAAAGAGCCCAGATGGAAGAAAATCCTCTGGAAGAGAATATTCTGGCCAGGGAAGAGTTGTCTCAA
  GCTGGTGACAGTAGTAACGAGGCAGTGGGCAGGGGGGACAGGCCAGATGCTGCCAGTCAAACCCCTCAGA
  CCTCAAGCGACTGGCTCGAGCAAGTACATTCAGTATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001003909
• Insert Size: 3258 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001003909.4, NP_001003909.2
• RefSeq Size: 6626 bp
• RefSeq ORF: 3258 bp
• Locus ID: 70797
• UniProt ID: Q6ZPS6
• Cytogenetics: 5 A1
• Gene Summary: Might act as an E3 ubiquitin-protein ligase, or as part of E3 complex, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes and then transfers it to substrates.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' region, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.