Fubp1 (NM_057172) Mouse Tagged ORF Clone

CAT#: MR217380

Fubp1 (Myc-DDK-tagged) - Mouse far upstream element (FUSE) binding protein 1 (Fubp1)

Product Images

Circular map for MR217380

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Fubp1
• Synonyms: 9530027K12Rik; D3Ertd330e; FBP; Fubp; Fubp4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR217380 representing NM_057172
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGACTACTCCACAGTGCCTCCGCCGTCGTCTGGCTCGGCTGGCGGCGGCGGCGGCGGAGTCGTTA
  ACGACGCTTTCAAAGATGCCCTGCAGAGAGCGCGGCAGATTGCAGCAAAAATTGGGGGTGATGCTGGTAC
  ATCATTGAATTCAAATGACTATGGTTATGGGGGACAAAAAAGACCCTTAGAAGATGGAGATCAGCCAGAT
  GCTAAGAAAGTACCTCCCCAAAATGACTCTTTTGGAGCACAGTTACCTCCAATGCATCAGCAGCAAAGAT
  CTGTAATGACAGAAGAATACAAAGTCCCAGATGGGATGGTCGGATTTATAATTGGCAGAGGAGGTGAACA
  GATCTCACGAATACAGCAGGAATCTGGATGCAAGATACAGATAGCACCTGATAGTGGTGGCCTACCAGAA
  AGGTCTTGTATGCTAACTGGAACACCTGAATCTGTCCAATCAGCAAAAAGATTATTGGACCAGATTGTTG
  AAAAGGGAAGACCAGCCCCTGGCTTTCATCATGGTGATGGACCTGGAAATGCAGTTCAGGAAATCATGAT
  TCCAGCCAGCAAAGCAGGACTAGTTATTGGAAAGGGGGGCGAGACTATTAAACAACTTCAGGAACGGGCT
  GGTGTTAAAATGGTAATGATTCAAGATGGGCCTCAAAACACTGGTGCTGATAAACCTCTTAGGATTACGG
  GTGACCCATACAAAGTTCAGCAAGCCAAGGAAATGGTATTAGAGTTAATTCGTGATCAAGGTGGTTTCAG
  AGAAGTGCGGAATGAGTATGGCTCAAGAATAGGAGGCAATGAAGGGATAGATGTCCCAATTCCAAGATTT
  GCTGTTGGCATTGTAATAGGAAGAAATGGAGAAATGATTAAAAAAATACAAAATGATGCTGGTGTTCGAA
  TTCAGTTTAAGCCAGATGATGGAACAACACCTGATAGGATAGCACAGATAACAGGACCTCCAGACAGGTG
  TCAGCACGCTGCAGAAATAATCACAGACCTTCTACGAAGTGTTCAGGCTGGCAATCCTGGTGGACCGGGA
  CCTGGTGGTCGAGGACGAGGTAGAGGTCAAGGAAACTGGAATATGGGGCCCCCGGGTGGACTCCAGGAGT
  TTAATTTCATTGTGCCAACTGGGAAAACTGGACTGATCATTGGAAAAGGAGGTGAAACCATAAAAAGCAT
  AAGCCAACAGTCTGGTGCAAGAATAGAACTGCAGAGAAGCCCTCCACCTAATGCAGATCCCAATATGAAG
  TTATTTACAATTCGGGGCACTCCACAGCAAATAGACTATGCTCGACAACTCATAGAAGAGAAGATTGGGG
  GCCCAGTAAATCCTTTAGGGCCACCTGTACCCCATGGGCCCCATGGGGTTCCAGGTCCTCATGGGCCTCC
  TGGACCTCCAGGGCCGGGAACTCCAATGGGACCATACAACCCTGCACCTTACAATCCAGGACCACCTGGC
  CCAGCTCCTCACGGTCCTCCAGCCCCATATGCTCCCCAGGGATGGGGAAATGCGTATCCACATTGGCAGC
  AACAGGCTCCTCCTGACCCAGCCAAGGCAGGAGCAGATCCAAACTCGGCAGCTTGGGCTGCTTATTATGC
  TCACTATTACCAGCAGCAGGCACAACCCCCACCTGCAGCTCCTGCCGGTGCACCAGCTACAACCCAAACG
  AACGGACAAGGTAACTACGGAGATCAGCAGGCTCCAGCTCCAGCTGGACAGGTTGATTATACAAAGGCTT
  GGGAAGAATACTACAAGAAAATGGGTCAAGCAGTTCCTGCTCCTGCTGGGGCCCCACCAGGTGGTCAGCC
  GGATTATAGTGCAGCCTGGGCTGAGTACTATAGACAGCAAGCAGCGTATTATGCCCAGACAAGTCCCCAG
  GGGATGCCGCAGCATCCTCCAGCACCTCAGGGCCAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR217380 representing NM_057172
  Red=Cloning site Green=Tags(s)
  
  MADYSTVPPPSSGSAGGGGGGVVNDAFKDALQRARQIAAKIGGDAGTSLNSNDYGYGGQKRPLEDGDQPD
  AKKVPPQNDSFGAQLPPMHQQQRSVMTEEYKVPDGMVGFIIGRGGEQISRIQQESGCKIQIAPDSGGLPE
  RSCMLTGTPESVQSAKRLLDQIVEKGRPAPGFHHGDGPGNAVQEIMIPASKAGLVIGKGGETIKQLQERA
  GVKMVMIQDGPQNTGADKPLRITGDPYKVQQAKEMVLELIRDQGGFREVRNEYGSRIGGNEGIDVPIPRF
  AVGIVIGRNGEMIKKIQNDAGVRIQFKPDDGTTPDRIAQITGPPDRCQHAAEIITDLLRSVQAGNPGGPG
  PGGRGRGRGQGNWNMGPPGGLQEFNFIVPTGKTGLIIGKGGETIKSISQQSGARIELQRSPPPNADPNMK
  LFTIRGTPQQIDYARQLIEEKIGGPVNPLGPPVPHGPHGVPGPHGPPGPPGPGTPMGPYNPAPYNPGPPG
  PAPHGPPAPYAPQGWGNAYPHWQQQAPPDPAKAGADPNSAAWAAYYAHYYQQQAQPPPAAPAGAPATTQT
  NGQGNYGDQQAPAPAGQVDYTKAWEEYYKKMGQAVPAPAGAPPGGQPDYSAAWAEYYRQQAAYYAQTSPQ
  GMPQHPPAPQGQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_057172
• ORF Size: 1926 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_057172.4
• RefSeq Size: 6491 bp
• RefSeq ORF: 1929 bp
• Locus ID: 51886
• Cytogenetics: 3 77.05 cM
• MW: 67.9 kDa
• Gene Summary: Regulates MYC expression by binding to a single-stranded far-upstream element (FUSE) upstream of the MYC promoter. May act both as activator and repressor of transcription (By similarity).[UniProtKB/Swiss-Prot Function]