Casz1 (NM_001159344) Mouse Tagged ORF Clone

CAT#: MR217174

Casz1 (Myc-DDK-tagged) - Mouse castor homolog 1, zinc finger (Drosophila) (Casz1), transcript variant 1

Product Images

Circular map for MR217174

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Casz1
• Synonyms: 2410019P08Rik; AV096627; BC040081; Cst; D4Ertd432e
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR217174 representing NM_001159344
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATCTTGGAACAGCTGAAAGCACCCGGTGCACCGACCCACCTGCAGGCAAGCCTCCAATGGCAGCCA
  AGCGCAAAGGCGGCCTGAAGCTCAACGCCATCTGTGCCAAGCTCAGCCGACAGGTGGTCGTGGAGAAGGG
  AGCAGAGGCCGGCTCCCAAGCCGAAGGTAGCCCACTACATCCCCGGGACAAAGAGCGCAGTGGCCCTGAG
  TCTGGGGTGAGCCGGGCTCCCCGAAGTGAAGAAGACAAGAGGCGGGCAGTGATCGAGAAATGGGTCAATG
  GAGAGTACTGTGAGGATCCCGCACCCACCCCAGTGTTGGGGCGTATTGCCCGTGATCAGGAGCTGCCCCC
  AGAGGGTGTCTACATGGTCCAGCCACAGGGCTGCAGTGACGAAGAAGACCATGCAGAAGAGCCCTCAAAA
  GATAACAGTGTCCTGGAGGAGAAGGAGTCAGATGGTACGGCTTCTAAAGATGACAGCGGCCCCAGCACCA
  GGCAGGCTTCAGGAGAAACCTCCTCTCTGAGGGACTACGCTGCTTCCACCATGACCGAGTTCCTCGGCAT
  GTTTGGCTACGATGACCAGAACACCAGGGATGAGCTGGCCAAGAAGATCAGCTTTGAGAAGCCGCATGCA
  GGCTCCACCCCCGAGGTGGCTGCCTCTTCCATGTTGCCCTCCTCTGAGGATACCCTCAGCAAGCGGGCGC
  GCTTCTCCAAATACGAGGAATACATCCGTAAGCTCAAGGCCGGCGAGCAACTTCCCTGGCCAGCCCACGG
  GAGCAAAGCCGAGGACCGGGCAGGCAAGGAGGTGGTGGGTCCCTTACCCAGCCTACGGCTGCCCAGCAAC
  ACGGCCCACCTGGAAACCAAGGCCACCATCCTGCCACTGCCATCACACAGCAGTGTCCAGATGCAGAATC
  TGGTAGCTCGTGCTTCCAAGTATGACTTCTTCATCCACAAACTGAAGACAGGCGAGAACCTGAGGCCCCA
  GAATGGAAGCACTTACAAGAAGCCATCCAAGTATGACCTGGAGAATGTCAAGTACTTGCACCTCTTCAAA
  CCCGGGGAAGGCAGCCCTGACATGGGCGGGGCCATCGCCTTCAAGACAGGCAAGGTGGGGCGCCCCTCTA
  AGTACGACGTTCGGGGCATCCAGAAGCCAGGCCCTACCAAGATTCCGCCCGCCCCCAGCCTGGTTCCTAC
  ACCCCTCACCAATGTGCCCAGTGCTCCCAGCACCCCCGGACCAGGACCGGAGCCACCTGCCTCCTTGTCC
  TTCAACACTCCCGAGTACCTGAAGTCAACCTTTTCCAAAACAGACTCCATCACCACAGGAACTGTCTCCA
  CTGTCAAGAACGGATTGCCCACAGATAAACCAGCTGTCACCGAAGATGTAAACATTTACCAGAAATATAT
  TGCCAGGTTCTCAGGAAGTCAGCACTGCGGTCACATCCACTGCGCCTACCAGTACCGTGAGCACTATCAC
  TGCCTGGACCCGGAGTGCAACTACCAGCGGTTCACAAGCAAGCAGGATGTGATCCGACATTACAACATGC
  ACAAGAAGCGCGACAACTCCCTGCAGCACGGCTTCATGCGCTTCAGCCCGCTGGACGACTGCAGTGTCTA
  CTACCACGGCTGCCACCTCAATGGGAAGAGCACCCACTACCACTGCATGCAGGTGGGATGTAACAAGGTA
  TACACAAGTACGTCGGATGTGATGACTCACGAGAACTTCCACAAGAAGAACACCCAGCTCATCAACGATG
  GCTTCCAGCGCTTCCGAGCCACGGAGGACTGCGGCACAGCTGACTGTCAGTTCTATGGACAGAAGACCAC
  ACACTTCCACTGCAGGCGCCCTGGCTGCACATTCACCTTCAAGAACAAGTGTGACATCGAGAAGCACAAG
  AGCTACCACATCAAGGATGATGCCTACGCCAAGGACGGCTTCAAGAAGTTCTACAAGTACGAGGAGTGCA
  AATACGAGGGCTGCATGTACAGCAAGGCCACCAACCATTTCCACTGCATCCGCGCCGGCTGCGGCTTCAC
  CTTCACCTCCACCAGCCAGATGACCTCACACAAGCGCAAGCACGAGCGGCGGCACATCCGGTCCTCGGGG
  GCCCTGGGGCTGCCGGCCTCCCTGCTGGGCGCCAAGGACACGGAGCACGAGGAATCCAGCAACGATGACC
  TCGTGGACTTCTCTGCCCTGAGCAGCAAGAACTCCAGCCTGAGCGCCTCCCCCACCAGCCAGCAATCGTC
  CGCATCCCTGGCCGCTGCGGCTGCCGCCACCACTGCTGAGGCCATCCCCAGTGCCACCAAGCCTCCCAAT
  AGCAAGATGGCAGGCCTGCTGCCCCAGGGCCTGTCTGGTTCCATCCCCTTAGCACTGGCCCTCTCTAACT
  CAGGCCTGCCCACCACCACACCCTATTTCCCTCTGCTTCCTAACCGTGGGAGCGCCTCATTGCCTGTGGG
  ATCTCCAGGGCTCCTGGGCTCCATGTCCTCTGGGGCCACAACCTCAGCAACCCCTGACATGCCGGCCCTG
  ATGGCTTCCAGAGCTGGAGACTCGGCCCCCACGGCTGCCACCTCTCTCTCGGTGCCCCCTGCCTCCATCA
  TTGAGAGAATCTCTGCAAGCAAAGGCCTCATCTCACCCATGATGGCTAGACTGGCTGCGGCCGCCCTCAA
  GCCCTCTGCCACCTTTGACCCAGGAAGTGGGCAGCAGCCCACCCCCACCAAGTTCCCCCAGGCCCAGGTG
  AAGCAGGAGCCTGACAGTGCTGGCACCCCAGGTCCCCACGAGGCCTCCCAAGACCGCAGTCTAGACCTGA
  CCGTGAAGGATCCCAGTAATGAATCAAATGGCCACGCAGTCTCGGCAAATTCATCTCTTTTATCCTCGCT
  TATGAATAAGATGTCTCAGGGCAACCCCAGCCTCGAAAGCTTCCTGAGCATCAAGACAGAAGCGGAGGGG
  AGCCCCGCCGGGGAGCCCTCGCCTTTCTTGGGCAAGGCCGTGAAGGCACTAGTTCAAGAGAAGCTGTCAG
  AGCCTTGGAAGGTGTATCTCCGCAGGTTTGGTACCAAGGACTTCTGTGATGCCCAGTGTGACTTCCTCCA
  CAAGGCGCATTTCCATTGTGTAGTGGAGGAGTGCGGTGCGCTTTTCAGCACCTTGGACGGAGCCATCAAA
  CATGCAAACTTCCACTTCCGGACAGAGGGAGGAACAGCAAAAGGAACCCCAGAGGCTTCCTTCCCGACCT
  CTGCTGCTGAGACCAAACCTCCCTTGGCACCCTCGTCCCTGCCAGCACCTCCTGGCACCATGGTCGCTGG
  ATCTTCTCTGGAGGGGCCTGCTCCCAGCCCGGTCTCTGTGCCCTCCACCCCCACCCTGCTCGCCTGGAAG
  CAGCTGGCTTCCACCATACCCCAGATGCCTCAGATTCCCTCCTCAGTGCCTCACCTGCCCACCTCGCCCC
  TGGCGACGACGTCTCTAGAGAGCGCCAAGCCTCAGGTCAAACCCGGGTTCCTCCAGTTCCAGGACAACGA
  TCCATGCCTGGCGACGGACTGCAAATACGCCAGCAAGTTCCACTTCCACTGTCTCTTCGGGAACTGCAAG
  TACGTCTGCAAGACCTCGGGCAAGGCTGAGTCCCACTGCTTGGACCACATCAACCCCAGCAACAGCCTGG
  TGAACGTGCGAGACCAGTTTGCTTACTACTCTCTACAGTGCCTGTGTCCCAACCAGCACTGTGAGTTCCG
  GATGCGTGGACACTACCACTGCCTCCGGACGGGCTGCTACTTTGTCACCAACATCACCACCAAACTTCCA
  TGGCATATAAAGAAGCATGAGAAGGCTGAACGACGGGCAGCCAACGGGTTCAAATACTTCACCAAGCGCG
  AGGAGTGCGGAAGACTAGGCTGCAAGTACAACCAGGTGAACAGCCACTTCCACTGCATCCGGGAAGGTTG
  TCAGTTCTCCTTTCTCCTCAAGCACCAGATGACCTCGCATGCCCGGAAGCACATGCGGAGGATGCTGGGG
  AAGAACTTTGACCGAGTGCCCCCATCCCAGGGTCCCCCGAGCCTAATGGATGCCGAGACAGACGAGGGCA
  TGGACTATACAGGCTGTAGCCCTGGAGCGGCGTCCTCAGAGTCTTCCACCATGGACCGTAGCTGTTCCAG
  CACCCCTGTGGGCAATGAGAGCACAGCTGCAGGGAACACCATCTCCATGCCGACAGCCTCGGGGGCCAAA
  AAGCGCTTTTGGATCATCGAGGACATGTCTCCCTTTGGCAAGCGAAGGAAGACAGCCTCGTCTCGGAAGA
  TGCTGGACGAGGGCATGATGCTGGAGGGTTTCCGGCGCTTTGACCTCTATGAGGACTGCAAGGATACAGC
  CTGCCAGTTCTCACTCAAGGTCACCCACTACCACTGCACGCGGGAAAACTGCGGCTACAAGTTTTGCGGA
  CGCACGCACATGTACAAGCACGCACAGCACCACGACCGCGTGGACAACCTGGTGCTGGACGACTTCAAGC
  GCTTCAAGGCCTCCCTCAGCTGCCACTTCGCCGACTGCCCGTTCTCGGGCACCAGCACGCACTTCCACTG
  CCTGCGCTGCCGCTTCCGTTGCACAGACAGCACCAAGGTCACTGCGCACCGCAAGCACCACGGCAAGCAG
  GACGTGATCAGCGCCGCTGGCTTCTGCCAGTTCAGCTCGAGCGCCGACTGCGCAGTGCCCGACTGCAAGT
  ACAAGCTCAAGTGCTCGCACTTCCACTGCACCTATCCGGGCTGTCGCCACACAGTGGTGGGCATGTCGCA
  GATGGACTCGCACAAGCGCAAGCACGAGAAGCAGGAGCGAGGGGAGCCTCCCGCAGCCTCTCCGGGTGCG
  CCTGTCAACCTGGACGGCTCCCTCACGCTGGCCGCCGAGCAGGGCTCGCTGCTCTTCCTGCAGACAGCGG
  CTGCGGGCCTAGGCCTGCTCGGGGACACGGGGGACCCCGGCCCACCTGTCACCGCCTCCGGGACTCGGGA
  CGGCCCCGCTGCGCCCACCCCCGCTGCCGCCGCCACCACCACCACCACCACCACAGCCACAGCCACAGCC
  ACCGCTGGGGAGTCCTCCCAAGAGGACGACGAGGAGCTGGAGCTGCCGGAGGAGGAGGCCGAGGACGACG
  ACGAGGATGACGATGAAGAGGACGACGACGACGAGGATGATGATGATGATGACGACGACGAGGACCTGCG
  CACCGACTCGGAGGAGTCGTTGCCCGAGGCTGCGGGCGAGGCGGGCGCGCGGACCCCGCTGGCAGCCCTG
  GGTGGCCCCGGCCCTGCGCCCACCGCCGCATCACCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR217174 representing NM_001159344
  Red=Cloning site Green=Tags(s)
  
  MDLGTAESTRCTDPPAGKPPMAAKRKGGLKLNAICAKLSRQVVVEKGAEAGSQAEGSPLHPRDKERSGPE
  SGVSRAPRSEEDKRRAVIEKWVNGEYCEDPAPTPVLGRIARDQELPPEGVYMVQPQGCSDEEDHAEEPSK
  DNSVLEEKESDGTASKDDSGPSTRQASGETSSLRDYAASTMTEFLGMFGYDDQNTRDELAKKISFEKPHA
  GSTPEVAASSMLPSSEDTLSKRARFSKYEEYIRKLKAGEQLPWPAHGSKAEDRAGKEVVGPLPSLRLPSN
  TAHLETKATILPLPSHSSVQMQNLVARASKYDFFIHKLKTGENLRPQNGSTYKKPSKYDLENVKYLHLFK
  PGEGSPDMGGAIAFKTGKVGRPSKYDVRGIQKPGPTKIPPAPSLVPTPLTNVPSAPSTPGPGPEPPASLS
  FNTPEYLKSTFSKTDSITTGTVSTVKNGLPTDKPAVTEDVNIYQKYIARFSGSQHCGHIHCAYQYREHYH
  CLDPECNYQRFTSKQDVIRHYNMHKKRDNSLQHGFMRFSPLDDCSVYYHGCHLNGKSTHYHCMQVGCNKV
  YTSTSDVMTHENFHKKNTQLINDGFQRFRATEDCGTADCQFYGQKTTHFHCRRPGCTFTFKNKCDIEKHK
  SYHIKDDAYAKDGFKKFYKYEECKYEGCMYSKATNHFHCIRAGCGFTFTSTSQMTSHKRKHERRHIRSSG
  ALGLPASLLGAKDTEHEESSNDDLVDFSALSSKNSSLSASPTSQQSSASLAAAAAATTAEAIPSATKPPN
  SKMAGLLPQGLSGSIPLALALSNSGLPTTTPYFPLLPNRGSASLPVGSPGLLGSMSSGATTSATPDMPAL
  MASRAGDSAPTAATSLSVPPASIIERISASKGLISPMMARLAAAALKPSATFDPGSGQQPTPTKFPQAQV
  KQEPDSAGTPGPHEASQDRSLDLTVKDPSNESNGHAVSANSSLLSSLMNKMSQGNPSLESFLSIKTEAEG
  SPAGEPSPFLGKAVKALVQEKLSEPWKVYLRRFGTKDFCDAQCDFLHKAHFHCVVEECGALFSTLDGAIK
  HANFHFRTEGGTAKGTPEASFPTSAAETKPPLAPSSLPAPPGTMVAGSSLEGPAPSPVSVPSTPTLLAWK
  QLASTIPQMPQIPSSVPHLPTSPLATTSLESAKPQVKPGFLQFQDNDPCLATDCKYASKFHFHCLFGNCK
  YVCKTSGKAESHCLDHINPSNSLVNVRDQFAYYSLQCLCPNQHCEFRMRGHYHCLRTGCYFVTNITTKLP
  WHIKKHEKAERRAANGFKYFTKREECGRLGCKYNQVNSHFHCIREGCQFSFLLKHQMTSHARKHMRRMLG
  KNFDRVPPSQGPPSLMDAETDEGMDYTGCSPGAASSESSTMDRSCSSTPVGNESTAAGNTISMPTASGAK
  KRFWIIEDMSPFGKRRKTASSRKMLDEGMMLEGFRRFDLYEDCKDTACQFSLKVTHYHCTRENCGYKFCG
  RTHMYKHAQHHDRVDNLVLDDFKRFKASLSCHFADCPFSGTSTHFHCLRCRFRCTDSTKVTAHRKHHGKQ
  DVISAAGFCQFSSSADCAVPDCKYKLKCSHFHCTYPGCRHTVVGMSQMDSHKRKHEKQERGEPPAASPGA
  PVNLDGSLTLAAEQGSLLFLQTAAAGLGLLGDTGDPGPPVTASGTRDGPAAPTPAAAATTTTTTTATATA
  TAGESSQEDDEELELPEEEAEDDDEDDDEEDDDDEDDDDDDDDEDLRTDSEESLPEAAGEAGARTPLAAL
  GGPGPAPTAASP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001159344
• ORF Size: 5286 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001159344.1, NP_001152816.1
• RefSeq Size: 7966 bp
• RefSeq ORF: 5289 bp
• Locus ID: 69743
• Cytogenetics: 4 78.87 cM
• MW: 191.7 kDa
• Gene Summary: Transcription factor involved in vascular assembly and morphogenesis through direct transcriptional regulation of EGFL7.[UniProtKB/Swiss-Prot Function]