Klhl40 (NM_028202) Mouse Tagged ORF Clone

CAT#: MR216861

Klhl40 (Myc-DDK-tagged) - Mouse kelch repeat and BTB (POZ) domain containing 5 (Kbtbd5)

Product Images

Circular map for MR216861

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Klhl40
• Synonyms: 2310024D23Rik; Kbtbd5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR216861 representing NM_028202
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACGCTGGGCTTGGAGCAGGCGGAGGAACAGCGTCTGTACCAGCAGACGCTCCTGCAAGACGGCCTCA
  AGGACATGCTGGACCACGGCAAGTTCCTAGACTGTGTAGTGCGCGTGGGTGAGCGCGAGTTCCCGTGCCA
  CCGCCTGGTGCTAGCCGCCTGTAGCCCCTACTTCCGCGCGCGCTTCCTGGCTGAGCCAGATAGCGCGGGA
  GAGGTACGCCTGGAGGAGGTGTCGCCAGACGTAGTGTCCCAGGTGCTGCACTACCTGTACACATCAGAGA
  TCGCGCTGGATGAGGCAAGCGTGCAAGACCTGTTTGCCGCGGCGCATCGATTCCAGATCCCGTCCATCTT
  CACCATCTGCGTGTCGTTTCTGCAGAAGCGCCTGTGCTTGGCCAATTGCCTGGCTGTCTTCCGCCTCGGC
  CTCCTGCTGGACTGCGCGCGTCTGGCTGTGGCCGCGCGCGACTTCATCTGCGCGCGCTTCCCGCTGGTGG
  CGCGGGACAACGACTTCCTGGGACTCTCGGCTGACGAGCTGATCGCCATCATCTCCAGCGACGGCCTCAA
  CGTAGAGAAGGAGGAGGCAGTGTTTGAGGCTGTGATGCGCTGGGCCAGCAGCGGAGATGCTGAGGCGCAG
  GCGGAGCGCCAGCGCGCGCTGCCCACGGTCTTCGAGAGCGTTCGCTGCCGCCTGCTGCCTCGTGCTTTCC
  TGGAGACTCGCGTGGAGCGCCACCCACTTGTGCGCTCCCAGCCCGAGCTGTTGCGCAAGGTGCAGATGGT
  GAAGGATGCACATGAGGGCCGCCTCACTACACTGCGCAAGAAGAAGAAGGAAAAGGGTGAGCAGACAGCT
  CGGGCCAAGGAGGCCAACCAGGGCACAGAAGACACCAAGGCTGAGGACGATGAGGAACGAGTACTGCCCG
  GGATCCTCAATGACACTCTGCGCTTCGGCATGTTCCTTCAGGATCTCATCTTCATGATCAGCGAGGAAGG
  CGCCGTGGCTTACGACCCAGCCGCCAACGAGTGCTACTGTGCATCCCTGTCCACCCAGATCCCCAAGAAC
  CATGTCAGTCTGGTGACCAAGGAGAACCAAGTCTTCGTGGCCGGTGGCCTCTTCTACAATGAGGACAACA
  AGGAGGACCCTATGAGTGCTTACTTCCTGCAGTTTGACCACTTGGACTCTGAGTGGCTGGGGATGCCGCC
  TCTCCCTTCACCTCGCTGCCTCTTCGGCCTGGGGGAGGCTCTCAACGCCATCTACGTGGTCGGCGGCCGG
  GAACTCAAGGACAGCGAAGACAGCCTGGACTCAGTCCTGTGCTACGACAGGCTGTCATTCAAATGGGGTG
  AGTCAGACCCGCTGCCCTACGCCGTGTACGGCCACACAGTCCTTTCCCACATGGACCTGGTCTATGTCAT
  TGGTGGCAAAGGCAAAGACAGGAAATGTCTGAACAAGATGTGTGTCTACGACCCCAAGAAGTTTGAGTGG
  AAGGAGCTAGCACCCATGCAGACAGCCCGATCGCTCTTTGGGGCCACGGTCCATGACGGCCGCATCTTTG
  TGGCCGCAGGGGTGACAGACACAGGACTTACCAGCTCTTCAGAGGTGTACAGCATTGCAGACAACAAGTG
  GACCTCCTTTGAGGCCTTCCCACAGGAACGAAGCTCGCTCAGCCTGGTCAGCCTAGCTGGCACTCTTTAT
  GCCTTGGGTGGCTTTGCCACTCTGGAGACGGAGTCTGGAGAGCTGGTCCCCACGGAGCTCAATGACATCT
  GGAGATACAACGAGGATGAGAAAAAGTGGGAGGGGGTCCTACGGGAGATCGCCTACGCAGCTGGCGCCAC
  CTTCCTCCCTGTGCGCCTCAATGTGCTTCGCCTGACCAAGATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR216861 representing NM_028202
  Red=Cloning site Green=Tags(s)
  
  MTLGLEQAEEQRLYQQTLLQDGLKDMLDHGKFLDCVVRVGEREFPCHRLVLAACSPYFRARFLAEPDSAG
  EVRLEEVSPDVVSQVLHYLYTSEIALDEASVQDLFAAAHRFQIPSIFTICVSFLQKRLCLANCLAVFRLG
  LLLDCARLAVAARDFICARFPLVARDNDFLGLSADELIAIISSDGLNVEKEEAVFEAVMRWASSGDAEAQ
  AERQRALPTVFESVRCRLLPRAFLETRVERHPLVRSQPELLRKVQMVKDAHEGRLTTLRKKKKEKGEQTA
  RAKEANQGTEDTKAEDDEERVLPGILNDTLRFGMFLQDLIFMISEEGAVAYDPAANECYCASLSTQIPKN
  HVSLVTKENQVFVAGGLFYNEDNKEDPMSAYFLQFDHLDSEWLGMPPLPSPRCLFGLGEALNAIYVVGGR
  ELKDSEDSLDSVLCYDRLSFKWGESDPLPYAVYGHTVLSHMDLVYVIGGKGKDRKCLNKMCVYDPKKFEW
  KELAPMQTARSLFGATVHDGRIFVAAGVTDTGLTSSSEVYSIADNKWTSFEAFPQERSSLSLVSLAGTLY
  ALGGFATLETESGELVPTELNDIWRYNEDEKKWEGVLREIAYAAGATFLPVRLNVLRLTKM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_028202
• ORF Size: 1863 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_028202.3, NP_082478.1
• RefSeq Size: 2455 bp
• RefSeq ORF: 1866 bp
• Locus ID: 72330
• UniProt ID: Q9D783
• Cytogenetics: 9 F4
• MW: 70.1 kDa
• Gene Summary: Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex that acts as a key regulator of skeletal muscle development (PubMed:25940086). The BCR(KLHL40) complex acts by mediating ubiquitination and degradation of TFDP1, thereby regulating the activity of the E2F:DP transcription factor complex (PubMed:25940086). Promotes stabilization of LMOD3 by acting as a negative regulator of LMOD3 ubiquitination; the molecular process by which it negatively regulates ubiquitination of LMOD3 is however unclear (PubMed:24960163).[UniProtKB/Swiss-Prot Function]