Klhl40 (NM_028202) Mouse Untagged Clone

CAT#: MC219836

Klhl40 (untagged) - Mouse kelch repeat and BTB (POZ) domain containing 5 (Kbtbd5), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Klhl40
• Synonyms: 2310024D23Rik; Kbtbd5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219836 representing NM_028202
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACGCTGGGCTTGGAGCAGGCGGAGGAACAGCGTCTGTACCAGCAGACGCTCCTGCAAGACGGCCTCA
  AGGACATGCTGGACCACGGCAAGTTCCTAGACTGTGTAGTGCGCGTGGGTGAGCGCGAGTTCCCGTGCCA
  CCGCCTGGTGCTAGCCGCCTGTAGCCCCTACTTCCGCGCGCGCTTCCTGGCTGAGCCAGATAGCGCGGGA
  GAGGTACGCCTGGAGGAGGTGTCGCCAGACGTAGTGTCCCAGGTGCTGCACTACCTGTACACATCAGAGA
  TCGCGCTGGATGAGGCAAGCGTGCAAGACCTGTTTGCCGCGGCGCATCGATTCCAGATCCCGTCCATCTT
  CACCATCTGCGTGTCGTTTCTGCAGAAGCGCCTGTGCTTGGCCAATTGCCTGGCTGTCTTCCGCCTCGGC
  CTCCTGCTGGACTGCGCGCGTCTGGCTGTGGCCGCGCGCGACTTCATCTGCGCGCGCTTCCCGCTGGTGG
  CGCGGGACAACGACTTCCTGGGACTCTCGGCTGACGAGCTGATCGCCATCATCTCCAGCGACGGCCTCAA
  CGTAGAGAAGGAGGAGGCAGTGTTTGAGGCTGTGATGCGCTGGGCCAGCAGCGGAGATGCTGAGGCGCAG
  GCGGAGCGCCAGCGCGCGCTGCCCACGGTCTTCGAGAGCGTTCGCTGCCGCCTGCTGCCTCGTGCTTTCC
  TGGAGACTCGCGTGGAGCGCCACCCACTTGTGCGCTCCCAGCCCGAGCTGTTGCGCAAGGTGCAGATGGT
  GAAGGATGCACATGAGGGCCGCCTCACTACACTGCGCAAGAAGAAGAAGGAAAAGGGTGAGCAGACAGCT
  CGGGCCAAGGAGGCCAACCAGGGCACAGAAGACACCAAGGCTGAGGACGATGAGGAACGAGTACTGCCCG
  GGATCCTCAATGACACTCTGCGCTTCGGCATGTTCCTTCAGGATCTCATCTTCATGATCAGCGAGGAAGG
  CGCCGTGGCTTACGACCCAGCCGCCAACGAGTGCTACTGTGCATCCCTGTCCACCCAGATCCCCAAGAAC
  CATGTCAGTCTGGTGACCAAGGAGAACCAAGTCTTCGTGGCCGGTGGCCTCTTCTACAATGAGGACAACA
  AGGAGGACCCTATGAGTGCTTACTTCCTGCAGTTTGACCACTTGGACTCTGAGTGGCTGGGGATGCCGCC
  TCTCCCTTCACCTCGCTGCCTCTTCGGCCTGGGGGAGGCTCTCAACGCCATCTACGTGGTCGGCGGCCGG
  GAACTCAAGGACAGCGAAGACAGCCTGGACTCAGTCCTGTGCTACGACAGGCTGTCATTCAAATGGGGTG
  AGTCAGACCCGCTGCCCTACGCCGTGTACGGCCACACAGTCCTTTCCCACATGGACCTGGTCTATGTCAT
  TGGTGGCAAAGGCAAAGACAGGAAATGTCTGAACAAGATGTGTGTCTACGACCCCAAGAAGTTTGAGTGG
  AAGGAGCTAGCACCCATGCAGACAGCCCGATCGCTCTTTGGGGCCACGGTCCATGACGGCCGCATCTTTG
  TGGCCGCAGGGGTGACAGACACAGGACTTACCAGCTCTTCAGAGGTGTACAGCATTGCAGACAACAAGTG
  GACCTCCTTTGAGGCCTTCCCACAGGAACGAAGCTCGCTCAGCCTGGTCAGCCTAGCTGGCACTCTTTAT
  GCCTTGGGTGGCTTTGCCACTCTGGAGACGGAGTCTGGAGAGCTGGTCCCCACGGAGCTCAATGACATCT
  GGAGATACAACGAGGATGAGAAAAAGTGGGAGGGGGTCCTACGGGAGATCGCCTACGCAGCTGGCGCCAC
  CTTCCTCCCTGTGCGCCTCAATGTGCTTCGCCTGACCAAGATGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_028202
• Insert Size: 1866 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_028202.3, NP_082478.1
• RefSeq Size: 2455 bp
• RefSeq ORF: 1866 bp
• Locus ID: 72330
• UniProt ID: Q9D783
• Cytogenetics: 9 F4
• Gene Summary: Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex that acts as a key regulator of skeletal muscle development (PubMed:25940086). The BCR(KLHL40) complex acts by mediating ubiquitination and degradation of TFDP1, thereby regulating the activity of the E2F:DP transcription factor complex (PubMed:25940086). Promotes stabilization of LMOD3 by acting as a negative regulator of LMOD3 ubiquitination; the molecular process by which it negatively regulates ubiquitination of LMOD3 is however unclear (PubMed:24960163).[UniProtKB/Swiss-Prot Function]