Smok3a (NM_001126045) Mouse Tagged ORF Clone

CAT#: MR216671

Smok3a (Myc-DDK-tagged) - Mouse sperm motility kinase 3A (Smok3a), transcript variant 2

Product Images

Circular map for MR216671

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Smok3a
• Synonyms: Smok2b1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR216671 representing NM_001126045
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCCCTGGGAGTCAACAGAAGTCAGAAAAGCTCAGATCCAAGTCCCCTTTGGCTGACATGGATGGTT
  TGCATGCTCAATATGTGATGCTAGAGACTATCGGCCATGGAGGCTGTGCCACAGTGAAGCTGGCCCAGCA
  CCGCCTCACAGGCACTCACGTGGCTGTCAAAACGATTCGAAAGAGGGAGTATTGGTGCAACCGTGTCATT
  TCTGAGGTAGAGTTACTGATGATGGCCGATCATCCGAATATCATCTCTCTCCTTCAAGTCATTGAGACCA
  AGAAGAAAGTATACCTCATTATGGAGTTGTGCAAGGGTAAATCACTTTACCAACACATCCGAAAAGCTGG
  CTACCTGCAGGAGCATGAAGCACGCGCATTATTCAAGCAGCTCTTAAGTGCTATGAACTACTGCCACAAC
  CAGGGTATAGTTCACAGGGACCTGAAACCGGACAATATCATGGTTGAAAAAGATGGGAAAGTGAAGATCA
  TTGATTTTGGACTCGGCACCAAAGTGAAGCCAGGGCAAAAACTCAACTTATTCTGTGGGACTTACCCATT
  TAGTGCTCCTGAGGTGCTCCTTAGCACACCCTATGATGGGCCCAAGATCGATGTATGGACTCTTGGAGTT
  GTGCTGTATTTTATGGTAACTGGAAAGATCCCGTTTGATGCTTGCAGCATAAAAAAGCTGGTAAAGCGAA
  TTCTTGCAGGAAAGTATTCTATTCCCTCTAGACTGTCAGCAGAGCTCCAAGACCTGCTTAGTCTTTTAAT
  GACGGCCAACCCCAAACTCAGGCCCACTGTTGCTGAGGTTATGGTGCATCCCTGGGTCACAGAAGGCTCA
  GGGGTGTTCCCAGATCCTTGTGAAGAACAGACCCCCCTCAAGCCAGACCCTGCAATTGTAAAAGCAATGG
  GACATATCGGGTTCCAAGCTCAAGATATTGAAGATTCGTTACGTCAGAGAAAATTCAACCAAACCATGGC
  GTCTTATTGTCTACTGAAAAAACAGATTCTTAAGGAATGTGACAGGCCAACCCGGGCTAGGCCCGTGAAC
  CCATCGGTGACCCCATTCCCTTCCCTTGTTGATACTGCTACTACCCGTCTCGGACTTCGCAGGAGAGAGA
  ATGAACCCACATGTCCCTGGTCATCCGCCAATAGGCAAGTGTCTGTCTGTGGCAAGAGTACTAGTAAGAA
  AAGAGACAGAAGAGTCAGTTGGCCCAGTGTTCTCGGCAGGCCACGCCACACGGCACCCACAATGGACCAC
  ACACGCACCCGTACTAGGAGTGTACCCTGCATTTGCTCAATGTTTTGCACAGTCCAGCCAAACAGCAGCG
  AAGAGAGCACACAAGGCCACACCAGAGCCTCAGCAGCAGATAAGCCTGTCCACAGCAGGGGCTGGCCCAG
  AGGCATCAAGGGCTGGACGAGGATGATAGGAAATGCGATGAGGAAGCTCTGTTGCTGTATCCCATCCAAA
  GAGACATCTCACCTGGGGCAGAACAGAGTCTCCCCCAAAAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR216671 representing NM_001126045
  Red=Cloning site Green=Tags(s)
  
  MGPGSQQKSEKLRSKSPLADMDGLHAQYVMLETIGHGGCATVKLAQHRLTGTHVAVKTIRKREYWCNRVI
  SEVELLMMADHPNIISLLQVIETKKKVYLIMELCKGKSLYQHIRKAGYLQEHEARALFKQLLSAMNYCHN
  QGIVHRDLKPDNIMVEKDGKVKIIDFGLGTKVKPGQKLNLFCGTYPFSAPEVLLSTPYDGPKIDVWTLGV
  VLYFMVTGKIPFDACSIKKLVKRILAGKYSIPSRLSAELQDLLSLLMTANPKLRPTVAEVMVHPWVTEGS
  GVFPDPCEEQTPLKPDPAIVKAMGHIGFQAQDIEDSLRQRKFNQTMASYCLLKKQILKECDRPTRARPVN
  PSVTPFPSLVDTATTRLGLRRRENEPTCPWSSANRQVSVCGKSTSKKRDRRVSWPSVLGRPRHTAPTMDH
  TRTRTRSVPCICSMFCTVQPNSSEESTQGHTRASAADKPVHSRGWPRGIKGWTRMIGNAMRKLCCCIPSK
  ETSHLGQNRVSPKK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001126045
• ORF Size: 1512 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001126045.1, NP_001119517.1
• RefSeq Size: 2160 bp
• RefSeq ORF: 1515 bp
• Locus ID: 545814
• UniProt ID: C0HKC9
• Cytogenetics: 5 G2
• MW: 56.5 kDa