Tspoap1 (NM_172449) Mouse Tagged ORF Clone

CAT#: MR216449

  • TrueORF®

Bzrap1 (Myc-DDK-tagged) - Mouse benzodiazepine receptor associated protein 1 (Bzrap1)

ORF Plasmid: DDK tGFP


  "NM_172449" in other vectors (2)


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Reconstitution Protocol

USD 1,551.00

In Stock*

Size
    • 10 ug

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Frequently bought together (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
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DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

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Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

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DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
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Other products for "Tspoap1"

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Symbol Tspoap1
Synonyms Bzrap1; D230016K05; mKIAA0612; PRAX-1
Vector pCMV6-Entry2
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR216449 representing NM_172449
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGCAACTGACAACCCTCCCACGGCTTGGAGACCTTGGAGCCATGGAGCCATGGGCACTGCCTGCCT
GGCAGCACTGGACTCAGGGCCAGGGGTGCAAACCTGGAGATGCATCTCCAAGCATTGCTGGTACTCCGAC
AGCTCTGCAGGTTAAAGGATTGAGGTTTGAAGAGAGTTCCAAGCCTGAGGGAGCTCATAGCCCTGGACCT
GTCGGAAATACTGATCCTGAAGCAACAGAGACCGGGCTGCCTAAGCTGGGGCAGCAAGCAGAGAGCCCTG
GGTACAGCTGTTCCGGGCTGGAGGAGGAGGAGGCACAGGCTTATAAGGCCAAGTTCAACATAGGCTTCGG
GGACAGGCCTAATCTGGAGCTGCTAAGGGCCCTGGGAGAGCTGCAGCAGCGCTGTACCATCCTGAAGGAG
GAAAACCAGATGCTGAGAAAAAGCAGCTTCCCAGAGACGGAGGAGAAGGTACGGAGGCTGAAGCGGAAGA
ATGCTGAACTGGCAGTCATTGCCAAGCGCCTGGAGGAGAGGGCACAGAAGCTGCAGGAAACGAACATGAG
GGTGGTGAGTGCCCCTGTGCCCCGACCCGGATCCAGTTTGGAGTTGTGCCGTAAGGCTCTAGCTCGCCAG
CGAGCCCGAGACCTCAGTGAGACAGCCAGTGCACTGTTGGCCAAGGACAAACAGATTGCTGCCTTGCAGC
GGGAGTGCAGGGAGCTGCAGGCCAGACTCTCTCTGGTGGGCAAGGAAGGTCCCCAGTGGCTGCATATGCG
GGACTTCGACCGGTTGCTGCGCGAGTCCCAACGGGAGGTGCTGCGGCTGCAGAGGCAGATCGCCCTGCGC
AACCAGCGGGAGCCGCTCCGGCCTGCCCGGTCCCCGGGTCCTACTGCCCCATCTAGAGTAGGGGCGCCGG
CCCCCGGGGCCCCGGGAGAGGCCGTACTTCAGGATGATGTGGAGAGCCCACAGGTAGTCCTAAGGGAACC
AGAGAAGCAGCAAAGGGTGCAGCAGCTGGAGTCTGAGCTCTGCAAGAAGCGAAAGAAATGCGAGAGTCTG
GAGCAGGAAGCCAGGAAAAAGCAGAGGCGATGTGAGGAGCTGGAGCTACAGCTGAGGGCAGCCCAGAATG
AAAATGCCCGCCTGGTGGAGGAGAACTCTCGGCTCAGTGGGAGAGCCACAGAGAAGGAGCAGGTGGAATG
GGAGAATTCGGAGCTGAAGGGACAGCTCCTGGGGGTGACACAAGAGAGGGACTCGGCCCTTCTTAAGAGC
CAGGGCCTGCAAAGCAAGCTGGAGAGCCTGGAGCAGGTGCTGAAGCATATGCGGGAGGTGGCCCAGCGCC
GGCAGCAGCTGGAGGTGGAGCATGAGCAGGCTAGGCTCAGCCTGCAGGAGAAGCAGGAGGAGGTCCGGAG
GCTGCAGCAGGCCCAGGCAGAAGCCAAGAGGGAACATGAGGGAGCCGTTCAGCTGCTGGAGTCTACCTTG
GATTCCATGCAGGCCCGGGTTCGAGAGCTTGAGGGCCAGTGCCGAAGCCAGACTGAGCGTTTCAGCCTTC
TGGCTCAGGAGCTCCAGGCCTTCCGTCTGCACCCAGGCCCTCTGGATCTGCTTACTTCAGCCTTGGGCTG
TAGTGCCCTTGGGGACCACCCGCCCCCCCACTGCTGCTGCTCTATCCCTCAGCCCTGCCAGGGGTCCGGC
CCCAAAGATCTTGACCTCCCACCGGGCTCCCCAGGACGCTGCACCCCCAAATCTTCTGAACCTGCTCTCA
CCACCCTTACTGGAATCCCTCGAAGGACAGCTAAGAAGGCCGAGTCTCTTTCTAATTCTTCTCGCTCAGA
GTCCATCCACAACAGCCCCAAGTCATGTCCCACACCAGAGGTGGACACAGCCAGTGAGGTGGAGGAACTG
GAGGTAGACAGTGTTTCCCTGCTCCCAGCAGCTCCGGAGAGTCACTCGGGAGGAGCCAGAATCCAGGTCT
TCCTAGCACGCTATAGCTACAACCCCTTTGAGGGTCCCAATGAGAATCCAGAGGCCGAGCTTCCTCTGAC
AGCGGGCGAGTATATCTACATCTATGGTAACATGGACGAGGATGGCTTTTTTGAAGGGGAGCTCATGGAT
GGCCGAAGGGGCCTGGTCCCTTCCAACTTTGTAGAGCGCGTGTCTGATGATGATCTTCTGTCCACCCTCC
CTCGGGAGCTGGCTGATTCGTCGCACAGCTCAGGCCCCGAGCTCAGTTTCCTGAGTGGAGGCGGGGGTGG
TTGCAGTAGTGGGGGCCAGAGCAGCGGGGGACGTAGCCAGCCCAGACCAGAGGAGGAGGCCGCAGGAGAT
GAACTCAGTCTGAGCCCCCCACCAGAGGGACTTGGCGAGCCTCTGGCTGTGCCTTACCCCCGACACATCA
CGGTTCTCAAGCAGTTAGCCCACAGTGTGGTGCTGGCCTGGGAGTTGCCTCCTGAGAGAGTAGATCTGCG
TGGCTTCCATATCTTTGTCAATGGGGAACTCCGTCAGGCCTTGGGGCCTGGGGTGCCCCCCAAAGCTGTG
CTTGAAAACATGGACCTGCGGACTGGGCCTCTCCATGTGTCTGTCCAGGCCCTAACCAGCAAGGGCAGCT
CAGACCCTCTGCGCTGTTGCCTGGCTGTGGGTGCCGGGGCTGGGGTGGTACCCAGCCAGCTGCGGATCCA
TCGGCTGACAGCCACATCTGCTGAGATCGCCTGGGTGCCGGGGAATAGCAACTTGGCCCATGCCATCTAC
CTCAATGGAGAAGAGTGCCCACCTGCTCGCCCCAGCACATATTGGGCAACCTTTTGTAACCTGAGACCTG
GCACACTCTATCAGGCCCGAGTGGAGGCTCAGATCCCATCTCAGGGGCCTTGGGAACCAGGCTGGGAGAG
GCCAGAGCAGAGAGCTGCTACCTTGCAGTTCACCACACTTCCAGCAGGTCTGCCTGATGCCCCACTGGAT
GTACAGGCTGAACCAGGACCCTCTCCTGGAATCTTGATGATCAGTTGGCTCCCTGTCACCATTGATGCTG
CTGGTACCTCCAATGGTGTCCGGGTTACAGGCTATGCCATCTATGCTGATGGGCAGAAGATTATGGAGGT
GGCTTCCCCCACAGCAGGCAGTGTGCTGGTGGAGGTGTCCCAGCTGCAGCTGTTGCAGGCCTGCCATGAG
GTGACTGTACGCACTATGTCACCCCATGGCGAGTCCAGTGACTCCATCCCGGCCCCCGTTGCCCCAGCCC
TGGCTTCTGCCTGCCAGCCAGCCAGGATGTCCTGTCTCTCACCACGACCAAGCCCAGAGGTCAGAACACC
CCTTGCTTCAGTCTCCCCAGGGCTTGGGGATACCAGCTTTCCCCTCCGGCATCCTGTCCCCCATGGAACT
CAAGATTTCTCTGCAAGTCTTTCCATAGAGATGTCCAAAGGACCCCAGGAGGAACCTCCAGTCCCTTGCT
CTCAGGAAGAGGCTGGGGCAGCTGTGCGGAGCATCTCAGAAGAGAAGAGGGCTATCGAGCCAACTCTGGG
CCAGGAAGGCCCTGAGCCTGTGGCTCCTTCCCTGGCTAAGCAGGAAGTGGAGTGCACTTCAGGAGATGCT
GGCCCTGTACCTTGCTCCACCCAAGGAGAGCTGACCCAGAAGAAGCCAAGTATTGAAGCCTGCCATGGGG
GAGATCTGGACTCCGGGCTGAAACTTAGATCTGAGAAAGAAGATATGTCAGAGCTTGGAGTTCACCTGGT
GAATTCCCTTGTGGATCACAGCCGCAACTCAGACTTATCAGACATCCAGGAAGAAGAGGAAGAGGAGGAA
GAAGAGGAAGAGGAACTGGGTTCCAGGCCTTGTTCCTCCCAGAAGCAGGTTGCTGGCAACAGCATCAGGG
AGAATGGAGCCAAGCCCCAGCCAGACCCCTTTTGTGAGACTGACAGCGACGAGGAGATCTTGGAGCAGAT
ACTGGAGTTGCCTCTCCAGCGGCTCTGCAGCAAGAAGCTGTTCAGCATCCCTGAGGAGGAAGAGGAGGAG
GAAGAGGAGGAAGGGCTGGAGAAACCAGGGCCCAGCCGCACTTCCCAAGACCCTAGCCAGCCTGAACTTG
CGTTGCTAGGGCCGGGCTGTGATAGCAGTCAGCCCCAGGGACCTGGCCTGTGTCCCTTGTCTCCTGAGCT
CTCTGGGGTCAGGGAGCACCTGGAGGATGTGCTGGGAGTCGTTGGTGGAAACGGCAGGAGGAGAGGAGGT
GGCTCCCCGGAGAAACTCCCAAACCGCAAGCGACCTCAGGACCCCCGAGAACATTGCAGCCGGCTTCTTG
GCAATGGCGGGCCCCAGGCCTCTGCACGGCCGGTCCCTCCACGGGAGAGGGGCAGCCTCCCTGTGATTGA
GGGCACCAGGGTTGGACAGGAGCCCGGTGGGAGAGGGCGGCCGGGTCTTTCCCGGAGGTGTCCCCGTGGC
CCTGCTCCAGAATCCAGCTTAGTCAGCTGCCTCTCTCCAAAGTGTTTGGAGATCAGTATTGAATATGATT
CTGAGGATGAGCAGGAGGCGGGCAGCGGGGGTGTCAGCATCAACAGCTCCTGCTACCCCACAGATGGGGA
GGCCTGGGGCACAGCGGCAGTAGGAAGGCCCAGGGGACCTCCGAAGGTCAATCCAGGCCCCAACGCCTAC
CTGCGCCTCCCAGCCTGGGAGAAAGGGGAGCCAGAGCGGAGAGGCCGCAGTGCGATTGGCAGAACCAAGG
AGCCACCCTCCCGGGCAACAGAAACTGGGGAGTCCAGAGGGCAGGACAACTCTGGGCGGAGAGGACCCCA
GAGGAGAGGGGCCCGGGTGCCTAGGTCTGGTACCACTGAGCTGGCCCCTCCAAGGAGCCCCCAAGAAGCA
CCACCTCATCAGGACCTGCCTGTGAGGGTCTTTGTGGCTCTGTTTGACTATGACCCTGTATCAATGTCAC
CGAACCCTGATGCCGGAGAAGAGGAACTGCCCTTTAAGGAGGGCCAACTCCTCAAGGTGTTTGGAGACAA
GGACGCTGATGGTTTCTACCGGGGTGAGAGTGGGGGCCGTACAGGCTACATCCCCTGCAACATGGTGGCT
GAGGTGGCTGTGGACAGTCCAGCAGGGAGACAACAGCTGCTCCAGCGGGGTTTCTTGCCCCCAAATGTTC
TCACCGAGGCCTCAGGAAATGGTCCCTCTGTGTACTCCTCAGCACACACACCCGGGCCTCCCCCCAAGCC
TCGTCGGTCCAAGAAAGTGGAGCTGGAAGGTCCTACACAGCTCTGTCCAGGTCCTCCTAAGCTGATTCAT
TCTGCTGCCCAGAAAACCTCCCGACCTATGGTGGCTGCATTTGACTATAATCCTCGGGAGAACTCCCCCA
ATATGGATGTGGAGGCAGAGCTGCCCTTCAGAGCAGGGGATGTCATTACTGTGTTTGGGAACATGGACGA
TGATGGTTTCTACTATGGGGAGCTGAATGGACAAAGGGGCCTGGTTCCATCCAACTTCCTGGAAGGCCCT
GGGCCTGAGTCAGGCAGCCTAGAGTCTGGGACATCTCAAGCCGAGAGTCAGAGAACGAGGAGGAGAAGAG
TCCAGTGC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR216449 representing NM_172449
Red=Cloning site Green=Tags(s)

MEQLTTLPRLGDLGAMEPWALPAWQHWTQGQGCKPGDASPSIAGTPTALQVKGLRFEESSKPEGAHSPGP
VGNTDPEATETGLPKLGQQAESPGYSCSGLEEEEAQAYKAKFNIGFGDRPNLELLRALGELQQRCTILKE
ENQMLRKSSFPETEEKVRRLKRKNAELAVIAKRLEERAQKLQETNMRVVSAPVPRPGSSLELCRKALARQ
RARDLSETASALLAKDKQIAALQRECRELQARLSLVGKEGPQWLHMRDFDRLLRESQREVLRLQRQIALR
NQREPLRPARSPGPTAPSRVGAPAPGAPGEAVLQDDVESPQVVLREPEKQQRVQQLESELCKKRKKCESL
EQEARKKQRRCEELELQLRAAQNENARLVEENSRLSGRATEKEQVEWENSELKGQLLGVTQERDSALLKS
QGLQSKLESLEQVLKHMREVAQRRQQLEVEHEQARLSLQEKQEEVRRLQQAQAEAKREHEGAVQLLESTL
DSMQARVRELEGQCRSQTERFSLLAQELQAFRLHPGPLDLLTSALGCSALGDHPPPHCCCSIPQPCQGSG
PKDLDLPPGSPGRCTPKSSEPALTTLTGIPRRTAKKAESLSNSSRSESIHNSPKSCPTPEVDTASEVEEL
EVDSVSLLPAAPESHSGGARIQVFLARYSYNPFEGPNENPEAELPLTAGEYIYIYGNMDEDGFFEGELMD
GRRGLVPSNFVERVSDDDLLSTLPRELADSSHSSGPELSFLSGGGGGCSSGGQSSGGRSQPRPEEEAAGD
ELSLSPPPEGLGEPLAVPYPRHITVLKQLAHSVVLAWELPPERVDLRGFHIFVNGELRQALGPGVPPKAV
LENMDLRTGPLHVSVQALTSKGSSDPLRCCLAVGAGAGVVPSQLRIHRLTATSAEIAWVPGNSNLAHAIY
LNGEECPPARPSTYWATFCNLRPGTLYQARVEAQIPSQGPWEPGWERPEQRAATLQFTTLPAGLPDAPLD
VQAEPGPSPGILMISWLPVTIDAAGTSNGVRVTGYAIYADGQKIMEVASPTAGSVLVEVSQLQLLQACHE
VTVRTMSPHGESSDSIPAPVAPALASACQPARMSCLSPRPSPEVRTPLASVSPGLGDTSFPLRHPVPHGT
QDFSASLSIEMSKGPQEEPPVPCSQEEAGAAVRSISEEKRAIEPTLGQEGPEPVAPSLAKQEVECTSGDA
GPVPCSTQGELTQKKPSIEACHGGDLDSGLKLRSEKEDMSELGVHLVNSLVDHSRNSDLSDIQEEEEEEE
EEEEELGSRPCSSQKQVAGNSIRENGAKPQPDPFCETDSDEEILEQILELPLQRLCSKKLFSIPEEEEEE
EEEEGLEKPGPSRTSQDPSQPELALLGPGCDSSQPQGPGLCPLSPELSGVREHLEDVLGVVGGNGRRRGG
GSPEKLPNRKRPQDPREHCSRLLGNGGPQASARPVPPRERGSLPVIEGTRVGQEPGGRGRPGLSRRCPRG
PAPESSLVSCLSPKCLEISIEYDSEDEQEAGSGGVSINSSCYPTDGEAWGTAAVGRPRGPPKVNPGPNAY
LRLPAWEKGEPERRGRSAIGRTKEPPSRATETGESRGQDNSGRRGPQRRGARVPRSGTTELAPPRSPQEA
PPHQDLPVRVFVALFDYDPVSMSPNPDAGEEELPFKEGQLLKVFGDKDADGFYRGESGGRTGYIPCNMVA
EVAVDSPAGRQQLLQRGFLPPNVLTEASGNGPSVYSSAHTPGPPPKPRRSKKVELEGPTQLCPGPPKLIH
SAAQKTSRPMVAAFDYNPRENSPNMDVEAELPFRAGDVITVFGNMDDDGFYYGELNGQRGLVPSNFLEGP
GPESGSLESGTSQAESQRTRRRRVQC

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NM_172449
ORF Size 5538 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_172449.2, NP_766037.2
RefSeq Size 7633 bp
RefSeq ORF 5541 bp
Locus ID 207777
UniProt ID Q7TNF8
Cytogenetics 11 C
MW 200.4 kDa

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