Galk2 (NM_175154) Mouse Tagged ORF Clone

CAT#: MR216381

Galk2 (Myc-DDK-tagged) - Mouse galactokinase 2 (Galk2)

Product Images

Circular map for MR216381

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Galk2
• Synonyms: 2810017M24Rik; AI327030; Gk2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR216381 representing NM_175154
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCGACCTATCTGAAAGATTGCTGAAGCTAAAAGAGATGTTTAACTCTAAATTTGGGTCCACACCCA
  AGTTTTATGTTCGAGCCCCAGGAAGAGTCAACATAATAGGTGAGCACATAGATTACTGTGGATATTCCGT
  TATTCCCATGGCTGTGGAACAAGACATGTTAATAGCTGTGGAACCTGTGAAAACACACACTCTCCAACTG
  GCTAATACAGACCCCTTGTATCCAGACTTCAGTACTACAGCTAATAACATCTGTATCGACAAGACCAAAC
  CTCTATGGCACAACTACTTCTTGTGTGGCTTTAAGGGAATTCAGGAACACTTTGGCCTTAGTAAGCTGCC
  TGGAATGAATTGCCTGGTAGATGGAAATATACCCCCGAGTTCGGGCCTCTCCAGCTCCAGTGCTCTGGTT
  TGCTGTGCTGGGTTAGTGACTCTCACGGTGCTGGGACTGAGACTTTCCAAGGTGGAACTTGCAGAAATCT
  GTGCCAAGAGTGAGCGCTACATAGGCACTGAAGGTGGCGGCATGGACCAGTCCATATCATTCCTTGCAGA
  AGAAGGAACTGCCAAGTTAATAGAATTTAGCCCTCTGAGAGCGACTAATGTGAAGCTCCCCAGTGGGGCA
  GTCTTTGTGATTGCCAACAGCTGCATGGAAATGAACAAGGCGGCCACTTCACATTTCAATGTCAGGGTGA
  TGGAGTGTCGACTGGCGGCAAAGGTCCTTGCTAAACACAAAGGCTTGCAATGGGATAATGTCCTGCGGCT
  AGAGGAAGTGCAAAGCAAACTCGGGATTAGTCTGGAAGAGATGCTGTTGGTCACAGAGGATGCCCTTCAT
  CCTGAACCCTACAGCCGGGAGGAGATCTGCAGGTGCCTGGGAATTAGCCTCGAGAGACTCCGCACCCAAA
  TCCTGACTCCAAATACTCAAGATGAACTCACCTTTAAGCTCTACCAGCGGGCCAAGCACGTGTACAGTGA
  GGCTGCACGCGTGCTGCAGTTTAAGCAGGTATGTGAAGATGCGCCAGACAACGCAGTCCAGCTGCTGGGG
  GAGCTAATGAACCAGAGCCACAGAAGCTGCCGGGACATGTATGAGTGCAGCTGCCCTGAGCTGGACCAGC
  TAGTGGACATCTGCCGGAAGTTTGGGGCCAAAGGCTCACGACTTACTGGAGCAGGATGGGGAGGCTGCAC
  TGTGTCCCTCGTGCCCGCTGACATGCTGTCCAGCTTTCTCGCCAGTGTGCACGAAGCTTATTACCAGGGG
  AACACATCCCGCCTGGCTCAGGAGAAGCATAGCTTGTTTGCCACCAAGCCTGGAGGCGGGGCCTTGGTTT
  TCCGTGAGGTC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR216381 representing NM_175154
  Red=Cloning site Green=Tags(s)
  
  MPDLSERLLKLKEMFNSKFGSTPKFYVRAPGRVNIIGEHIDYCGYSVIPMAVEQDMLIAVEPVKTHTLQL
  ANTDPLYPDFSTTANNICIDKTKPLWHNYFLCGFKGIQEHFGLSKLPGMNCLVDGNIPPSSGLSSSSALV
  CCAGLVTLTVLGLRLSKVELAEICAKSERYIGTEGGGMDQSISFLAEEGTAKLIEFSPLRATNVKLPSGA
  VFVIANSCMEMNKAATSHFNVRVMECRLAAKVLAKHKGLQWDNVLRLEEVQSKLGISLEEMLLVTEDALH
  PEPYSREEICRCLGISLERLRTQILTPNTQDELTFKLYQRAKHVYSEAARVLQFKQVCEDAPDNAVQLLG
  ELMNQSHRSCRDMYECSCPELDQLVDICRKFGAKGSRLTGAGWGGCTVSLVPADMLSSFLASVHEAYYQG
  NTSRLAQEKHSLFATKPGGGALVFREV
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_175154
• ORF Size: 1341 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_175154.3, NP_780363.1
• RefSeq Size: 2520 bp
• RefSeq ORF: 1344 bp
• Locus ID: 69976
• Cytogenetics: 2 F1
• MW: 49.8 kDa
• Gene Summary: Acts on GalNAc. Also acts as a galactokinase when galactose is present at high concentrations (By similarity).[UniProtKB/Swiss-Prot Function]