Enpep (NM_007934) Mouse Tagged ORF Clone

CAT#: MR216141

Enpep (Myc-DDK-tagged) - Mouse glutamyl aminopeptidase (Enpep)

Product Images

Circular map for MR216141

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Enpep
• Synonyms: 6030431M22Rik; APA; Bp-1/6C3; Ly-51; Ly51
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR216141 representing NM_007934
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACTTTGCAGAGGAAGAGCCCTCCAAGAAATACTGCATTAAAGGCAAACACGTGGCCATCATCTGTG
  GAGTAGTGGTGGCAGTTGGATTAATAGTGGGACTTTCTGTGGGTTTGACCAGGTCGTGTGAGCAGGACAC
  AACCCCAGCTCCTTCCCAACCTCCTCCGGAAGCCAGCACTGCCCTCCCTCCTCAGGACCAGAATGTCTGC
  CCTGACAGTGAAGATGAAAGCGGAGAATGGAAAAACTTCAGGCTGCCTGACTTCATCAATCCAGTTCACT
  ACGACCTGGAGGTGAAGGCCCTGATGGAGGAAGACAGGTACACGGGAATAGTGACCATCTCTGTCAACTT
  GAGCAAACCCACTCGTGACCTATGGCTCCACATCAGGGAGACCAAGATCACCAAGCTGCCGGAGCTAAGG
  AGGCCCTCTGGGGAGCAGGTACCAATTCGACGGTGCTTCGAGTATAAGAAGCAGGAGTACGTGGTGATCC
  AGGCTGCAGAAGACCTTGCGGCCACCAGTGGGGACAGTGTCTATCGGCTGACCATGGAGTTTAAAGGCTG
  GCTGAACGGTTCCCTTGTGGGTTTTTACAAAACCACCTACATGGAGGACGGGCAAATCAGGAGCATAGCT
  GCCACTGACCATGAACCAACAGATGCCAGGAAGTCCTTCCCTTGTTTCGACGAACCCAACAAGAAGTCAA
  CTTACAGTATATCCATCATCCACCCAAAAGAATACTCAGCACTTTCTAATATGCCAGAAGAGAAATCAGA
  GATGGTGGATGACAACTGGAAGAAAACCACTTTTGTGAAGTCTGTCCCAATGAGCACTTACCTGGTGTGC
  TTTGCTGTGCATCGGTTCACTGCTATAGAGAGAAAATCCAGGAGCGGCAAACCACTCAAGGTCTATGTCC
  AGCCCAATCAGAAGGAGACAGCAGAGTATGCGGCAAACATAACCCAAGCTGTATTTGATTACTTCGAAGA
  CTACTTCGCTATGGAGTATGCGCTTCCTAAACTGGATAAAATTGCTATTCCAGATTTTGGCACCGGCGCC
  ATGGAAAATTGGGGACTTGTCACTTACCGAGAAACAAACCTGCTTTACGACCCCCTACTATCGGCCTCAT
  CTAACCAGCAGAGAGTGGCCAGCGTGGTTGCCCACGAACTTGTACACCAGTGGTTTGGAAATACTGTGAC
  CATGGACTGGTGGGACGACTTGTGGCTAAATGAAGGATTTGCTTCGTTCTTCGAGTTCTTGGGAGTAAAC
  CACGCAGAAAAAGACTGGCAGATGCTCAGTCAGGTGCTGCTTGAAGATGTGTTCCCCGTGCAAGAGGACG
  ACTCCCTGATGTCTTCACATCCAGTGGTCGTCACCGTGTCCACGCCAGCTGAAATAACATCTGTGTTTGA
  TGGGATATCATACAGCAAGGGAGCTTCTATTCTGAGAATGCTCCAAGACTGGATAACACCAGAGAAATTC
  CAAAAAGGCTGTCAGATTTACTTGAAAAAATTCCAGTTCGCGAATGCAAAAACTTCCGACTTTTGGGATT
  CACTGCAAGAGGCAAGCAATCTGCCAGTGAAAGAAGTGATGGACACCTGGACTAGCCAGATGGGTTATCC
  TGTGGTCACTGTGAGTGGAAGGCAGAACATCACCCAGAAACGCTTTCTGTTGGACTCCAAAGCTGATCCT
  TCGCAGCCACCGTCAGAGCTCGGTTACACATGGAATATTCCAGTCAGATGGGCTGATAATGACAACTCAA
  GGATCACCGTGTACAATAGGTTAGACAAAGGAGGAATCACTCTGAATGCTAATCTTAGCGGAGATGCTTT
  TCTCAAAATCAACCCAGATCACATTGGGTTTTATCGTGTAAATTATGAAGGAGGAACGTGGGATTGGATA
  GCCGAGGCTCTCTCCTCAAACCACACGAGATTCTCCGCTGCTGACCGGTCAAGTTTTATTGATGATGCTT
  TTGCTTTGGCAAGAGCTCAACTTCTGAATTATAAAATAGCTCTGAACTTGACCATGTATCTCAAATCAGA
  AGAGGATTTCCTACCATGGGAGAGAGTCATTTCATCTGTAAGCTACATCATTAGCATGTTTGAAGATGAC
  AGAGAGCTGTACCCCATGATAGAGACGTACTTTCAAGGCCAAGTGAAGCCCGTTGCAGATTTGCTGGGAT
  GGCAGGATACCGGAAGCCACATCACAAAGTTACTCCGGGCCTCTATCTTAGGATTCGCATGCAAGATGGG
  GGACAGAGAAGCCTTGGGCAATGCTTCCCAGTTATTTGACTCCTGGCTGAAAGGGAGCGCAAGTATTCCG
  GTAAACCTCAGGCTGCTTGTGTACCGCTATGGAATGCAGAACTCTGGCAATGAGGCAGCGTGGAACTATA
  CCCTAGAGCAGTATCAGAAAACATCGCTTGCCCAAGAGAAAGAAAAACTGCTCTATGGGTTAGCTTCAGT
  GAAGGATGTTAAACTCTTGGCAAGGTATCTGGAAATGCTCAAAGACCCCAATATTATTAAAACTCAGGAT
  GTATTTACCGTCATCCGCTACATCTCCTACAACAGTTATGGGAAGACAATGGCCTGGAATTGGATACAAC
  TCAACTGGGACTATCTGGTCAGCAGATTTACAATCAATGACAGATACCTTGGCCGGATCGTCACCATAGC
  TGAGCCCTTCAACACTGAACTGCAGCTCTGGCAGATGCAGAGCTTTTTTGCAAAATATCCAAATGCTGGC
  GCTGGAGCAAAACCTAGAGAGCAAGTGCTGGAGACGGTGAAGAACAACATCGAGTGGCTAAACGTGAACA
  GACAGTCCATCAGAGAGTGGTTCGCTAGCCTGCCG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR216141 representing NM_007934
  Red=Cloning site Green=Tags(s)
  
  MNFAEEEPSKKYCIKGKHVAIICGVVVAVGLIVGLSVGLTRSCEQDTTPAPSQPPPEASTALPPQDQNVC
  PDSEDESGEWKNFRLPDFINPVHYDLEVKALMEEDRYTGIVTISVNLSKPTRDLWLHIRETKITKLPELR
  RPSGEQVPIRRCFEYKKQEYVVIQAAEDLAATSGDSVYRLTMEFKGWLNGSLVGFYKTTYMEDGQIRSIA
  ATDHEPTDARKSFPCFDEPNKKSTYSISIIHPKEYSALSNMPEEKSEMVDDNWKKTTFVKSVPMSTYLVC
  FAVHRFTAIERKSRSGKPLKVYVQPNQKETAEYAANITQAVFDYFEDYFAMEYALPKLDKIAIPDFGTGA
  MENWGLVTYRETNLLYDPLLSASSNQQRVASVVAHELVHQWFGNTVTMDWWDDLWLNEGFASFFEFLGVN
  HAEKDWQMLSQVLLEDVFPVQEDDSLMSSHPVVVTVSTPAEITSVFDGISYSKGASILRMLQDWITPEKF
  QKGCQIYLKKFQFANAKTSDFWDSLQEASNLPVKEVMDTWTSQMGYPVVTVSGRQNITQKRFLLDSKADP
  SQPPSELGYTWNIPVRWADNDNSRITVYNRLDKGGITLNANLSGDAFLKINPDHIGFYRVNYEGGTWDWI
  AEALSSNHTRFSAADRSSFIDDAFALARAQLLNYKIALNLTMYLKSEEDFLPWERVISSVSYIISMFEDD
  RELYPMIETYFQGQVKPVADLLGWQDTGSHITKLLRASILGFACKMGDREALGNASQLFDSWLKGSASIP
  VNLRLLVYRYGMQNSGNEAAWNYTLEQYQKTSLAQEKEKLLYGLASVKDVKLLARYLEMLKDPNIIKTQD
  VFTVIRYISYNSYGKTMAWNWIQLNWDYLVSRFTINDRYLGRIVTIAEPFNTELQLWQMQSFFAKYPNAG
  AGAKPREQVLETVKNNIEWLNVNRQSIREWFASLP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007934
• ORF Size: 2835 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007934.3, NP_031960.1
• RefSeq Size: 4208 bp
• RefSeq ORF: 2838 bp
• Locus ID: 13809
• UniProt ID: P16406
• Cytogenetics: 3 57.92 cM
• MW: 108.4 kDa
• Gene Summary: Regulates central hypertension through its calcium-modulated preference to cleave N-terminal acidic residues from peptides such as angiotensin II.[UniProtKB/Swiss-Prot Function]