Jmjd7 (NM_001114637) Mouse Tagged ORF Clone

CAT#: MR216128

  • TrueORF®

Jmjd7 (Myc-DDK-tagged) - Mouse jumonji domain containing 7 (Jmjd7)

ORF Plasmid: DDK tGFP

Lentiviral Particles: DDK w/ Puro mGFP w/ Puro

AAV Particle: DDK


  "NM_001114637" in other vectors (4)


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Reconstitution Protocol

USD 300.00

In Stock*

Size
    • 10 ug

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Frequently bought together (4)
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Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
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DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
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Other products for "Jmjd7"

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Symbol Jmjd7
Synonyms MGC106779
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR216128 representing NM_001114637
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGAGGCGGCTCTGGAGGCAGTTCGGAGGGCGTTGCAAGAGTTCCCGGCGGCGGCTCGCGACCTCA
ATGTACCTCGTGTTGTGCCCTACCTGGATGAGCCCCCAAGCCCACTCTGCTTCTACCGGGATTGGGTGTG
CCCCAACAGGCCCTGCATTATCCGAAATGCTCTGCAGCACTGGCCAGCCCTCCAGAAGTGGTCCCTGTCC
TACTTAAGAGCCACGGTGGGCTCCACGGAGGTGAGTGTGGCTGTGACTCCAGATGGTTATGCGGACGCGG
TGCGAGGGGACCGCTTTGTGATGCCTGCTGAACGCCGCCTGCCCATAAGCCATGTACTGGATGTGTTGGA
AGGTCGGGCCCAGCACCCAGGAGTCCTCTATGTGCAGAAACAGTGTTCCAACCTGCCCACTGAGCTGCCC
CAGCTTCTGTCTGACATTGAGTCCCATGTGCCCTGGGCCTCTGAGTCACTGGGGAAGATGCCTGATGCCG
TGAACTTCTGGCTGGGTGATGCATCTGCAGTGACATCCTTGCACAAGGACCACTATGAGAATCTGTACTG
TGTAGTCTCTGGCGAGAAACACTTCTTGTTACATCCACCCAGCGACCGGCCCTTCATCCCTTACAATCTC
TACACACCAGCAACCTACCAGCTGACTGAAGAGGGCACTTTTAGGGTGGTGGACGAGGAAGCCATGGAGA
AGGTACCCTGGATCCCACTGGACCCCTTGGCTCCAGACCTGACCCAGTACCCCAGTTACAGCCAGGCACA
GGCCCTTCACTGCACAGTGCGGGCCGGCGAGATGCTGTACCTGCCAGCTCTGTGGTTCCACCATGTCCAG
CAGTCCCACGGCTGTATTGCTGTGAATTTCTGGTATGACATGGAGTATGACCTCAAGTACAGTTACTTTC
AACTGATGGACACCCTCACCAGGGCCACAGGCCTTGAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR216128 representing NM_001114637
Red=Cloning site Green=Tags(s)

MAEAALEAVRRALQEFPAAARDLNVPRVVPYLDEPPSPLCFYRDWVCPNRPCIIRNALQHWPALQKWSLS
YLRATVGSTEVSVAVTPDGYADAVRGDRFVMPAERRLPISHVLDVLEGRAQHPGVLYVQKQCSNLPTELP
QLLSDIESHVPWASESLGKMPDAVNFWLGDASAVTSLHKDHYENLYCVVSGEKHFLLHPPSDRPFIPYNL
YTPATYQLTEEGTFRVVDEEAMEKVPWIPLDPLAPDLTQYPSYSQAQALHCTVRAGEMLYLPALWFHHVQ
QSHGCIAVNFWYDMEYDLKYSYFQLMDTLTRATGLD

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001114637
ORF Size 948 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001114637.1, NP_001108109.1
RefSeq Size 1383 bp
RefSeq ORF 951 bp
Locus ID 433466
UniProt ID P0C872
Cytogenetics 2
MW 35.9 kDa
Gene Summary Bifunctional enzyme that acts both as an endopeptidase and 2-oxoglutarate-dependent monoxygenase (PubMed:28847961) (By similarity). Endopeptidase that cleaves histones N-terminal tails at the carboxyl side of methylated arginine or lysine residues, to generate 'tailless nucleosomes', which may trigger transcription elongation (PubMed:28847961). Preferentially recognizes and cleaves monomethylated and dimethylated arginine residues of histones H2, H3 and H4. After initial cleavage, continues to digest histones tails via its aminopeptidase activity (PubMed:28847961). Additionally, may play a role in protein biosynthesis by modifying the translation machinery. Acts as Fe(2+) and 2-oxoglutarate-dependent monoxygenase, catalyzing (S)-stereospecific hydroxylation at C-3 of 'Lys-22' of DRG1 and 'Lys-21' of DRG2 translation factors (TRAFAC), promoting their interaction with ribonucleic acids (RNA) (By similarity).[UniProtKB/Swiss-Prot Function]

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