Lrrc32 (NM_001113379) Mouse Tagged ORF Clone

CAT#: MR215360

Lrrc32 (Myc-DDK-tagged) - Mouse leucine rich repeat containing 32 (Lrrc32)

Product Images

Circular map for MR215360

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Lrrc32
• Synonyms: AI426318; D7H11S833E; D11S833Eh; EG434215; Garp
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR215360 representing NM_001113379
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCCACCAGATCCTGCTACTCCTGGCCATGCTGACCCTGGGCCTGGCTATCTCTCAGCGTCGAGAGC
  AAGTGCCCTGTAGGACGGTGAACAAGGAGGCCTTGTGCCACGGCCTTGGCCTGCTCCAGGTCCCCTCGGT
  GCTCTCACTGGACATCCAAGCTCTCTACTTGTCCGGGAACCAACTGCAGAGCATCCTGGTCTCCCCACTG
  GGCTTCTATACAGCGCTTCGTCACCTGGATTTAAGTGACAACCAGATTAGCTTCCTCCAGGCTGGGGTCT
  TCCAGGCCCTGCCCTACCTGGAGCACCTTAACCTGGCCCACAATCGGCTTGCCACAGGCATGGCTCTCAA
  CAGTGGTGGTCTGGGCCGCCTGCCCCTTTTGGTCTCCCTGGACCTGTCTGGGAACAGCCTGCATGGCAAT
  CTGGTGGAGCGACTGTTGGGTGAGACCCCGAGGCTGCGTACGCTCTCACTGGCGGAGAACAGCCTCACTC
  GCCTGGCACGCCACACCTTCTGGGGTATGCCAGCGGTGGAGCAATTAGACCTCCACAGCAATGTCCTCAT
  GGATATCGAGGACGGTGCCTTTGAGGCCCTGCCCCACCTGACTCACCTCAATCTCTCCAGAAACTCCCTC
  ACCTGCATCTCAGACTTCAGCCTCCAGCAGCTGCAGGTACTAGACTTGAGCTGCAACAGCATTGAGGCCT
  TCCAGACGGCCCCGGAACCACAGGCCCAGTTCCAGTTGGCCTGGCTCGACCTCCGGGAGAACAAGCTGCT
  CCACTTCCCTGACCTGGCCGTGTTCCCGAGACTCATCTACCTGAATGTGTCTAACAACCTCATCCAGCTC
  CCTGCGGGGCTGCCCCGGGGCAGTGAGGACCTCCACGCACCCTCTGAAGGCTGGTCAGCCTCTCCACTGT
  CCAACCCCAGCCGGAATGCCAGCACCCACCCTCTCTCCCAGCTCCTGAACCTGGATCTGAGTTACAATGA
  GATCGAACTGGTCCCTGCTAGCTTTCTTGAACACCTGACCTCCCTGCGCTTCCTCAACCTCAGCAGAAAC
  TGCCTGCGATCCTTTGAGGCTCGACAAGTGGACTCCCTGCCCTGCCTGGTGCTTTTGGACTTGAGCCACA
  ATGTGCTGGAAGCATTAGAACTGGGTACCAAAGTCCTAGGGTCCCTGCAGACGTTGCTTCTGCAGGACAA
  TGCCTTGCAAGAACTTCCACCCTATACCTTTGCCAGCCTGGCCAGCCTGCAGAGGCTAAACCTACAGGGG
  AACCAGGTCAGTCCCTGTGGGGGGCCAGCAGAACCAGGTCCCCCGGGCTGTGTGGACTTCTCTGGGATCC
  CCACCCTTCATGTTCTTAACATGGCAGGGAACTCGATGGGAATGCTCAGGGCGGGCAGCTTCCTTCACAC
  CCCACTTACAGAGCTGGACCTCTCCACCAATCCTGGTCTGGATGTGGCCACGGGAGCCCTGGTGGGCCTG
  GAGGCATCCTTAGAGGTACTGGAGCTTCAAGGCAACGGGCTGACTGTCTTGAGAGTGGACTTGCCCTGCT
  TTCTCCGTCTCAAGCGCCTTAACCTTGCTGAGAACCAGTTAAGCCACCTGCCCGCTTGGACGCGGGCTGT
  GTCCCTGGAGGTGCTAGATCTGCGGAACAACAGCTTCAGCCTCCTGCCAGGCAATGCCATGGGTGGCCTG
  GAGACCAGTCTCCGGCGGCTGTACTTGCAGGGAAATCCACTCAGCTGCTGTGGCAATGGCTGGCTGGCGG
  CCCAGCTGCACCAGGGCAGAGTGGATGTGGATGCTACTCAGGACCTAATCTGCCGCTTTGGCTCCCAGGA
  GGAGTTGTCCCTGAGCCTAGTGCGTCCAGAGGATTGCGAGAAGGGAGGGCTGAAGAATGTCAACCTCATC
  CTCCTCCTCAGCTTCACACTGGTCTCTGCCATCGTCCTCACCACGCTGGCCACCATCTGCTTCCTCCGCC
  GGCAGAAGCTCAGCCAACAATACAAAGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR215360 representing NM_001113379
  Red=Cloning site Green=Tags(s)
  
  MSHQILLLLAMLTLGLAISQRREQVPCRTVNKEALCHGLGLLQVPSVLSLDIQALYLSGNQLQSILVSPL
  GFYTALRHLDLSDNQISFLQAGVFQALPYLEHLNLAHNRLATGMALNSGGLGRLPLLVSLDLSGNSLHGN
  LVERLLGETPRLRTLSLAENSLTRLARHTFWGMPAVEQLDLHSNVLMDIEDGAFEALPHLTHLNLSRNSL
  TCISDFSLQQLQVLDLSCNSIEAFQTAPEPQAQFQLAWLDLRENKLLHFPDLAVFPRLIYLNVSNNLIQL
  PAGLPRGSEDLHAPSEGWSASPLSNPSRNASTHPLSQLLNLDLSYNEIELVPASFLEHLTSLRFLNLSRN
  CLRSFEARQVDSLPCLVLLDLSHNVLEALELGTKVLGSLQTLLLQDNALQELPPYTFASLASLQRLNLQG
  NQVSPCGGPAEPGPPGCVDFSGIPTLHVLNMAGNSMGMLRAGSFLHTPLTELDLSTNPGLDVATGALVGL
  EASLEVLELQGNGLTVLRVDLPCFLRLKRLNLAENQLSHLPAWTRAVSLEVLDLRNNSFSLLPGNAMGGL
  ETSLRRLYLQGNPLSCCGNGWLAAQLHQGRVDVDATQDLICRFGSQEELSLSLVRPEDCEKGGLKNVNLI
  LLLSFTLVSAIVLTTLATICFLRRQKLSQQYKA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001113379
• ORF Size: 1989 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001113379.2
• RefSeq Size: 3817 bp
• RefSeq ORF: 1992 bp
• Locus ID: 434215
• UniProt ID: G3XA59
• Cytogenetics: 7 53.86 cM
• MW: 72.9 kDa
• Gene Summary: Key regulator of transforming growth factor beta (TGFB1, TGFB2 and TGFB3) that controls TGF-beta activation by maintaining it in a latent state during storage in extracellular space (PubMed:25127859). Associates specifically via disulfide bonds with the Latency-associated peptide (LAP), which is the regulatory chain of TGF-beta, and regulates integrin-dependent activation of TGF-beta (PubMed:25127859, PubMed:28912269). Able to outcompete LTBP1 for binding to LAP regulatory chain of TGF-beta (By similarity). Controls activation of TGF-beta-1 (TGFB1) on the surface of activated regulatory T-cells (Tregs) (PubMed:25127859). Required for epithelial fusion during palate development by regulating activation of TGF-beta-3 (TGFB3) (PubMed:28912269).[UniProtKB/Swiss-Prot Function]