Adam4 (NM_009620) Mouse Tagged ORF Clone

CAT#: MR215306

Adam4 (Myc-DDK-tagged) - Mouse a disintegrin and metallopeptidase domain 4 (Adam4)

Product Images

Circular map for MR215306

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Adam4
• Synonyms: AV274654; tMDCV
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR215306 representing NM_009620
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCCCTTCCTCAGACCATCTACCTGGACTTTGGCCCTCCTGAGAGGTGCTCTCTGGCTCTCTGTGC
  TCTGGGCACTGTTGTCTCCTGCCTGCTGTTCCCGTAGCCCACCCAAATGGCGCTTCTCTACCTCTGAAAT
  TGTCATCCCCAGGAAGGTTCCCCAGAGAATGGGCAAAAGTGATATGTCTGGGCACATCACCTATAGCATG
  CGCTTTAGGGGACAAAGACATGTGGTCCACATGAAACTCAAGAAAAACATGATTCCTCAAAATTTTCCTG
  TATATACCTCTAATGACCAAGGAGCCCAACAGAAGGATTACCCATTTGTTCCTCGGGATTGTTACTTCTA
  CAGCTTCCTGGAAGGAGTCCCTGGGTCCCAAGCTATACTAGATACCTGTACTGGAGGTCTGAAAGGCATG
  ATACAGGTGGATGACTTCACTTATGAAATCAAACCATTGGCATCTTCTTCCAAATTTGAGCATGTAATTT
  CTCTGCTTGTGGTGGATGAAAGGTCACGTGAGTCAAAAAAGTGTAGAAATGATGAGATTATGGCAAAGGC
  AGGTGACCTCTCTGAAGAGACCAAGCTGGCTGGAAGTCCCAGAGCAGCCCCTGTATATCTGTGGCGTTAC
  CATTTGAAAAGTGTAGGAATACACTACACGGTGACTCATGACTTATTCAAACAAACTGGGAGCAATTCCT
  CCACCTCAGTTGAGTTAATACTGATTATGAATAGCATTTCAGATAGCATTTATAAAATAAGTGGATTGAT
  TGTGTATGCACGTGGTGTATGGATTTGGAATACGAAGAACCTGCATACTGTCCCCGGTGAAAATCCCCAC
  CTTGATCCTTGGAGTGTTATGGCAGGTTTTGGTGTATGGAAATCTAAAGAATTGCACGATTTGTATGCCA
  CTACAGCTATTCTCCTTGCATCAAGACCAAAAAATACAGATTATGGAAGCTTTCAGAATGGATTATGTAA
  CCCCAACTGGGGAGTATTATTTACATATGTAGGAAAAAACCATTTATTTCTGGCTGGATCTTTCCTGGCA
  CATGCAGTGGGTCACCTTTTAGATGTAAGCCATGATACACCAGGCTGTGTTTGTTTCAGAAGGAGCAGTT
  GTCTCATGGATGAATTTCCTACTCTTCATGATATGATTAGTAATTGTTCCCATAATGAGTTACATAGGAG
  GATCCATGACTGGGACCCTTGCTTGAGTTTAGAGCTAAAAACAGCTAAAGAAGTAAACATGTATGAAGTT
  CCTCGTTGTGGAAATAAGAGAGTGGAGGCCAGTGAGAAATGTGACTGTGGTTCTGTGAAAGACTGTACCA
  CTGATAAGTGTTGTGAAGTCAATTGTGAGTTTACTCAGGGTAGCTCTTGTGCTGCAGGAGGTTGCTGTCT
  ATCTTGTAAGTTTGCACCCACTGGAACAATATGCAGAGACAAAAATGGTATTTGTGATCTTCCAGAGTAC
  TGTAGTGGAGCCTCAGAGCATTGCCCAGGAAATTTTTACATCATGGATGGAACTCCTTGTTCTCCACTGG
  CAGTCTGCATAGCAGGAAACTGTAGTGACCGTCATTTGCAGTGTCAAGCTCTTTTTGGATACCAAGTAAA
  GGATGGTTCACCAGCATGCTATAATGAATTAAATGTCAAAGGTGACCGATTTGGAAACTGTGGCATTAGA
  ATCATACGTGGAGGAAGCCAACCTGTCCCATGTCAAAAGGAGGATGTTTTTTGTGGAATGATCCACTGTG
  ATGGTGTCAGTCATATTCCTGGTGGAGGTGAGCACACTACCTTTTATCACCTAAAGGTTCAAGATGTTAA
  AGAAGAACAGTGCTTTGGGTATGATGCACACCATGGGACAGAACTTCCAGAAATGGGGCTTGTAGTGGAT
  GGTGCAACCTGTGGCCCTGGCAAATATTGTAAAGCTCAAAGATGTGTTGCTCATCCAACTTTGAATTTTA
  ATTGCAACATTAGTTCGTGTAACTTCAGAGGGGTGTGCAACAACAAAGGCAACTGTCACTGTGTTCGAGG
  ATGGCAGCCACCACATTGTTTACAAATAGGAAAAGGTGGTAGTACAAATAGTGGCCCTCCACCTACCACT
  CAGAAAAAACATCAAGCTGAAATACATGTGAGTGCCAACAGGCTAATAGTCATTTTAAGTATTCGCATGA
  TTCTTATCTTGGCTTCGATTCTTTTGGGTGCAGTTTTCAAAGCAATATTTGTTTCAGAAACTACAGAAGA
  GGAGCAACATTCTGCTGTTAACAGACAGTTGACTTCATACCAAACTATT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR215306 representing NM_009620
  Red=Cloning site Green=Tags(s)
  
  MAPFLRPSTWTLALLRGALWLSVLWALLSPACCSRSPPKWRFSTSEIVIPRKVPQRMGKSDMSGHITYSM
  RFRGQRHVVHMKLKKNMIPQNFPVYTSNDQGAQQKDYPFVPRDCYFYSFLEGVPGSQAILDTCTGGLKGM
  IQVDDFTYEIKPLASSSKFEHVISLLVVDERSRESKKCRNDEIMAKAGDLSEETKLAGSPRAAPVYLWRY
  HLKSVGIHYTVTHDLFKQTGSNSSTSVELILIMNSISDSIYKISGLIVYARGVWIWNTKNLHTVPGENPH
  LDPWSVMAGFGVWKSKELHDLYATTAILLASRPKNTDYGSFQNGLCNPNWGVLFTYVGKNHLFLAGSFLA
  HAVGHLLDVSHDTPGCVCFRRSSCLMDEFPTLHDMISNCSHNELHRRIHDWDPCLSLELKTAKEVNMYEV
  PRCGNKRVEASEKCDCGSVKDCTTDKCCEVNCEFTQGSSCAAGGCCLSCKFAPTGTICRDKNGICDLPEY
  CSGASEHCPGNFYIMDGTPCSPLAVCIAGNCSDRHLQCQALFGYQVKDGSPACYNELNVKGDRFGNCGIR
  IIRGGSQPVPCQKEDVFCGMIHCDGVSHIPGGGEHTTFYHLKVQDVKEEQCFGYDAHHGTELPEMGLVVD
  GATCGPGKYCKAQRCVAHPTLNFNCNISSCNFRGVCNNKGNCHCVRGWQPPHCLQIGKGGSTNSGPPPTT
  QKKHQAEIHVSANRLIVILSIRMILILASILLGAVFKAIFVSETTEEEQHSAVNRQLTSYQTI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009620
• ORF Size: 2289 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009620.2, NP_033750.1
• RefSeq Size: 2330 bp
• RefSeq ORF: 2292 bp
• Locus ID: 11498
• Cytogenetics: 12 D1
• MW: 84.4 kDa