Cdkl3 (NM_001166656) Mouse Tagged ORF Clone

CAT#: MR214810

Cdkl3 (Myc-DDK-tagged) - Mouse cyclin-dependent kinase-like 3 (Cdkl3), transcript variant 5

Product Images

Circular map for MR214810

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cdkl3
• Synonyms: AI852479; B230379H01Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR214810 representing NM_001166656
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGATGTATGAAACCCTTGGAAAAGTGGGAGAGGGAAGTTATGGAACGGTCATGAAATGCAAACATA
  AGGACACTGGGCGAATAGTGGCCATTAAGATTTTCTATGAGAAACCAGAAAAATCTGTCAACAAAATTGC
  AACGAGAGAAATAAAGTTTCTAAAGCAATTTCGGCATGAAAACCTGGTCAATTTGATTGAAGTTTTTAGA
  CAAAAGAAGAAAATTCATTTGGTATTTGAGTTTATTGACCACACGGTCTTAGATGAGCTGCAGCACTACT
  GTCACGGACTAGAGAGTAAGCGGCTGAGAAAGTACCTCTTCCAGATCCTTCGCGCCATTGAGTACCTGCA
  TAATAATAATATTATCCATCGAGATATAAAGCCTGAGAATATTTTAGTCTCCCAGTCAGGAATTACGAAG
  CTGTGTGATTTTGGGTTTGCGAGGACACTAGCAGCTCCTGGAGACGTTTACACAGACTACGTGGCCACAC
  GCTGGTACAGAGCTCCAGAGCTGGTGTTGAAAGACACCTCCTACGGAAAGCCAGTGGACATCTGGGCTTT
  GGGCTGTATGATCATTGAAATGGCCACTGGCCATCCCTTCCTTCCTAGCAGTTCCGATTTGGATTTGCTC
  CACAAGATTGTTTTAAAAGTAGGCAACCTGACCCCGCACCTGCACAATATCTTTTCCAAGAGTCCCATCT
  TTGCTGGGGTGGTTCTTCCTCAAGTTCAACACCCAAAAACCGCAAGAAAGAAATACCCCAAGCTCAATGG
  ATTGCTGGCAGATATAGTTCATGCTTGTTTACAAATTGATCCTGCTGAGAGGACATCATCTACTGATCTT
  TTGCGTCACGATTACTTTACTAGAGATGGATTTATTGAGAAATTCATACCAGAGCTGAGAGCTAAATTAT
  TACAGGAAGCAAAGGTTAATTCATTTATAAAGCCAAAAGAGAATTTTAAAGAAAATGAACCTGTGAGAGA
  TGAGAAGAAATCAGTTTTTACCAACACCCTGCTCTATGGAAATCCATCACTTTATGGCAAGGAAGTGGAC
  AGAGACAAAAGGGCCAAGGAGCTCAAAGTCAGAGTCATTAAGGCCAAAGGGGGCAAAGGAGATGTCCCAG
  ACCAGAAGAAGCCAGAGTATGAAGGCGACCACCGCCAGCAGGGCACAGCTGATGACACACAGCCCTCATC
  ACTGGACAAGAAGCCTTCTGTCTTGGAACTGACAAACCCTCTCAATCCCAGTGAGAATTCTGACGGTGTC
  AAAGAAGACCCACACGCTGGGGGTTGTATGATAATGCCACCTATCAACCTGACAAGCAGTAATTTGTTGG
  CCGCAAATCTCAGTTCAAACCTTTCCCACCCCAATTCACGGTTAACTGAAAGAACAAAAAAGAGACGCAC
  TTCTTCACAAACTATTGGACAGACTTTGTCTAATAGCAGACAAGAGGACACAGGTCCCACACAAGTCCAA
  ACAGAGAAAGGTGCATTTAATGAGCGAACAGGTCAGAATGACCAAATATCGAGTGGGAACAAAAGAAAGC
  TGAATTTTCCCAAATGCGACAGGAAAGAATTCCATTTCCCTGAACTGCCATTCACAGTGCAGGCGAAGGA
  GATGAAAGGGATGGAAGTTAAACAGATAAAAGTGCTGAAGAGAGAATCAAAGAAAACAGATTCATCTAAA
  ATACCAACTTTACTTAGTATGGACCCAAATCAAGAAAAACAAGAGAGTCTCACTATGTACCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR214810 representing NM_001166656
  Red=Cloning site Green=Tags(s)
  
  MEMYETLGKVGEGSYGTVMKCKHKDTGRIVAIKIFYEKPEKSVNKIATREIKFLKQFRHENLVNLIEVFR
  QKKKIHLVFEFIDHTVLDELQHYCHGLESKRLRKYLFQILRAIEYLHNNNIIHRDIKPENILVSQSGITK
  LCDFGFARTLAAPGDVYTDYVATRWYRAPELVLKDTSYGKPVDIWALGCMIIEMATGHPFLPSSSDLDLL
  HKIVLKVGNLTPHLHNIFSKSPIFAGVVLPQVQHPKTARKKYPKLNGLLADIVHACLQIDPAERTSSTDL
  LRHDYFTRDGFIEKFIPELRAKLLQEAKVNSFIKPKENFKENEPVRDEKKSVFTNTLLYGNPSLYGKEVD
  RDKRAKELKVRVIKAKGGKGDVPDQKKPEYEGDHRQQGTADDTQPSSLDKKPSVLELTNPLNPSENSDGV
  KEDPHAGGCMIMPPINLTSSNLLAANLSSNLSHPNSRLTERTKKRRTSSQTIGQTLSNSRQEDTGPTQVQ
  TEKGAFNERTGQNDQISSGNKRKLNFPKCDRKEFHFPELPFTVQAKEMKGMEVKQIKVLKRESKKTDSSK
  IPTLLSMDPNQEKQESLTMYL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001166656
• ORF Size: 1743 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001166656.1, NP_001160128.1
• RefSeq Size: 2030 bp
• RefSeq ORF: 1746 bp
• Locus ID: 213084
• UniProt ID: Q8BLF2
• Cytogenetics: 11 B1.3
• MW: 66.6 kDa