AU022751 (NM_001166433) Mouse Tagged ORF Clone

CAT#: MR214772

AU022751 (Myc-DDK-tagged) - Mouse expressed sequence AU022751 (AU022751), transcript variant 1. Note: ORF is codon optimized

Product Images

Circular map for MR214772

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: AU022751
• Synonyms: AU023811
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR214772 representing NM_001166433, codon optimized.
  Due to the complexity of NM_001166433, the ORF clone is codon optimized for mammalian Expression.
  The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical.
  
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAGCTCCTCCTCCCCAGAGCGGGGGCTGGAAGCACTGCGAGATACTGATGAGAGCGAGGGCGAAG
  CACCTGGACCGTCAGGCCCGAGAGGCAGGGGAGGGCCTTCAGGGGCCGGTTCTGCACTTCGGTTGAGATC
  CCTGGAAGCTGAAATGGCCGCCGCTTGCGTTACCTCAACTGCAGGTGAGGACTTGGGAACCTTTTCCGAG
  CCAGGCTCACAGCATGGGGATCCCGAGGGCGGGGGTGGGCCTGACCTTGAACTGGGGCACGCTAGACCAA
  TGATGCGGAGTCAAAGAGAGTTGGGCCTCACACCTAAAGGCGGAGGAAAGGCAGATCAGGGCGGGAAGGG
  CAGGAAGGGCGGGAGCGGTAGCCCCCCTCATACCAAGAGCTCCCGAAAGAGGGAACAGCCTAACCCCAAT
  CGATCACTGATGGCTCAGGGGGCAGCTGGACCCCCATTGCCCGGCGCACGAGGCAGTCCTGCTATGCCCC
  AACCCGAGAGTAGCCTCTCTCCCAGGCCTGATCAGTCTCACCATTTTGACTTCCCCGTCGGGAATCTGGA
  GGCACCCGGGCCAACCCTGAGGTCCAGTACCAGTCAGGGATCTGGGAGCACCCCCGTGCCAGAAGCCCTG
  AGGTGCGCTGAGAGTAGCCGAGCCGAAAGCGACCAGTCTAGTCCCGCGGGCAGAGAGCTTCGGCAGCAGG
  CTTCTCCCCGAGCCCCTGACGATGATGATGATGGAGATGGAGGCCCTGATCCACGGGGCAGCGGGACACC
  AGAAGGATGGGTACTGCGCAGCGGTGTGGTGCCTTTCGGTAGGCGCAGCTCTGCCAGTGAGGTGAGTCCC
  GAGGAGGTGAGGCCTGAGGCTCAATGTACAGGCTGGAACCTCAGACCGCGGCCAAGATCAAGCGCTTCAG
  CAGTGTCACCAGAGGCTCGGCCTAAAGCGCAATCCGCCGGTAGAAATCTGCGGCCTCGGCCGCGCAGTAG
  TGCCTCAGTGGTCAGTCCGGAAGCACGGCCCAAAGCACAGAGTGCTGGCCGCAACCTCCGCCCAAGACCC
  AGGAGCTCTGCGTCAGTTGTATCTCCTGAGGCCAGGCCCGAGGCACAGAGCGCCGGACGCAATCTTCGAC
  CTCGAGCCACCCCCAGAGTTCCTGTCGCACCGAGCTCTACCACTAGAAGTTCCAGTGACAGAGGTTCTTC
  ACGCGCCCCCAGATCTAGGTCCAGATCCAGATCATGCAGCACACCTCGGCTGGGGTCTGATCATCAGAGA
  TCTCGAAAAATTAAGATGCGACTGGACCTGCAAGTCGATCGGGAACCGGAGTCCGAAGCGGAGCAAGAGG
  AACAGGAACTTGAGTCTGAGCCCGGTCCCTCCTCCCGCCCACAGGCTTCTAGAAGCAGCAGCAGATTTGC
  CGTCCCCGGGAGATCTTCTCTGGCCGCAGAAGATTCACCGCCTAGAAGACCGGTCCGGATGAGGGCTAGC
  TCTCCCTCACCACCAGGTCGCTTGTACCCACTCCCTAAGCACTACTTCGAAGGGGTGCACTCCCCAAGCT
  CATCTAGTAGCGAGAGCTCTTCCGTTAGTAGCAGTCATAGCCCCCTCAATAAGGCTCCTGACCCTGGTTC
  CTCCCCTCCTCTGAGTAGCCTTTCAGGTCCTAACCCTTTCTGGTTGGCTCTGATCGCCGACCTGGACAAT
  CTGGATTCCAGCAGTCCCAGGGTGCCTGGCGAGGAGATCGAGGCTGCACCTCACACACGAGAGGAGGAAG
  ATAAGAAGTGCCGGGGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR214772 representing NM_001166433
  Red=Cloning site Green=Tags(s)
  
  MASSSSPERGLEALRDTDESEGEAPGPSGPRGRGGPSGAGSALRLRSLEAEMAAACVTSTAGEDLGTFSE
  PGSQHGDPEGGGGPDLELGHARPMMRSQRELGLTPKGGGKADQGGKGRKGGSGSPPHTKSSRKREQPNPN
  RSLMAQGAAGPPLPGARGSPAMPQPESSLSPRPDQSHHFDFPVGNLEAPGPTLRSSTSQGSGSTPVPEAL
  RCAESSRAESDQSSPAGRELRQQASPRAPDDDDDGDGGPDPRGSGTPEGWVLRSGVVPFGRRSSASEVSP
  EEVRPEAQCTGWNLRPRPRSSASAVSPEARPKAQSAGRNLRPRPRSSASVVSPEARPKAQSAGRNLRPRP
  RSSASVVSPEARPEAQSAGRNLRPRATPRVPVAPSSTTRSSSDRGSSRAPRSRSRSRSCSTPRLGSDHQR
  SRKIKMRLDLQVDREPESEAEQEEQELESEPGPSSRPQASRSSSRFAVPGRSSLAAEDSPPRRPVRMRAS
  SPSPPGRLYPLPKHYFEGVHSPSSSSSESSSVSSSHSPLNKAPDPGSSPPLSSLSGPNPFWLALIADLDN
  LDSSSPRVPGEEIEAAPHTREEEDKKCRG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001166433
• ORF Size: 1767 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001166433.1, NP_001159905.1
• RefSeq Size: 2203 bp
• RefSeq ORF: 1770 bp
• Locus ID: 102991
• UniProt ID: Q3UWZ4
• Cytogenetics: X A1.1
• MW: 61.8 kDa
• Gene Summary: Inhibits PRC2/EED-EZH1 and PRC2/EED-EZH2 complex function by inhibiting EZH1/EZH2 methyltransferase activity, thereby causing down-regulation of histone H3 trimethylation at 'Lys-27' (H3K27me3) (PubMed:31086175). Probably inhibits methyltransferase activity by limiting the stimulatory effect of cofactors such as AEBP2 and JARID2 (By similarity). Inhibits H3K27me3 deposition during spermatogenesis and oogenesis (PubMed:31451685).[UniProtKB/Swiss-Prot Function]