AU022751 (NM_001166433) Mouse Tagged ORF Clone

CAT#: MG214772

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  • TrueORF®

AU022751 (GFP-tagged) - Mouse expressed sequence AU022751 (AU022751) transcript variant 1, (10ug). Note: ORF is codon optimized



  "NM_001166433" in other vectors (2)

USD 680.00


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Size
    • 10 ug


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Specifications

Product Data
Product Name AU022751 (NM_001166433) Mouse Tagged ORF Clone
Type Mouse Tagged ORF Clone
Tag TurboGFP
Symbol AU022751
Synonyms AU023811
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Cell Selection Neomycin
Sequence Data
>MG214772 representing NM_001166433, codon optimized.
Due to the complexity of NM_001166433, the ORF clone is codon optimized for mammalian Expression.
The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical
.

Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCAGCTCCTCCTCCCCAGAGCGGGGGCTGGAAGCACTGCGAGATACTGATGAGAGCGAGGGCGAAG
CACCTGGACCGTCAGGCCCGAGAGGCAGGGGAGGGCCTTCAGGGGCCGGTTCTGCACTTCGGTTGAGATC
CCTGGAAGCTGAAATGGCCGCCGCTTGCGTTACCTCAACTGCAGGTGAGGACTTGGGAACCTTTTCCGAG
CCAGGCTCACAGCATGGGGATCCCGAGGGCGGGGGTGGGCCTGACCTTGAACTGGGGCACGCTAGACCAA
TGATGCGGAGTCAAAGAGAGTTGGGCCTCACACCTAAAGGCGGAGGAAAGGCAGATCAGGGCGGGAAGGG
CAGGAAGGGCGGGAGCGGTAGCCCCCCTCATACCAAGAGCTCCCGAAAGAGGGAACAGCCTAACCCCAAT
CGATCACTGATGGCTCAGGGGGCAGCTGGACCCCCATTGCCCGGCGCACGAGGCAGTCCTGCTATGCCCC
AACCCGAGAGTAGCCTCTCTCCCAGGCCTGATCAGTCTCACCATTTTGACTTCCCCGTCGGGAATCTGGA
GGCACCCGGGCCAACCCTGAGGTCCAGTACCAGTCAGGGATCTGGGAGCACCCCCGTGCCAGAAGCCCTG
AGGTGCGCTGAGAGTAGCCGAGCCGAAAGCGACCAGTCTAGTCCCGCGGGCAGAGAGCTTCGGCAGCAGG
CTTCTCCCCGAGCCCCTGACGATGATGATGATGGAGATGGAGGCCCTGATCCACGGGGCAGCGGGACACC
AGAAGGATGGGTACTGCGCAGCGGTGTGGTGCCTTTCGGTAGGCGCAGCTCTGCCAGTGAGGTGAGTCCC
GAGGAGGTGAGGCCTGAGGCTCAATGTACAGGCTGGAACCTCAGACCGCGGCCAAGATCAAGCGCTTCAG
CAGTGTCACCAGAGGCTCGGCCTAAAGCGCAATCCGCCGGTAGAAATCTGCGGCCTCGGCCGCGCAGTAG
TGCCTCAGTGGTCAGTCCGGAAGCACGGCCCAAAGCACAGAGTGCTGGCCGCAACCTCCGCCCAAGACCC
AGGAGCTCTGCGTCAGTTGTATCTCCTGAGGCCAGGCCCGAGGCACAGAGCGCCGGACGCAATCTTCGAC
CTCGAGCCACCCCCAGAGTTCCTGTCGCACCGAGCTCTACCACTAGAAGTTCCAGTGACAGAGGTTCTTC
ACGCGCCCCCAGATCTAGGTCCAGATCCAGATCATGCAGCACACCTCGGCTGGGGTCTGATCATCAGAGA
TCTCGAAAAATTAAGATGCGACTGGACCTGCAAGTCGATCGGGAACCGGAGTCCGAAGCGGAGCAAGAGG
AACAGGAACTTGAGTCTGAGCCCGGTCCCTCCTCCCGCCCACAGGCTTCTAGAAGCAGCAGCAGATTTGC
CGTCCCCGGGAGATCTTCTCTGGCCGCAGAAGATTCACCGCCTAGAAGACCGGTCCGGATGAGGGCTAGC
TCTCCCTCACCACCAGGTCGCTTGTACCCACTCCCTAAGCACTACTTCGAAGGGGTGCACTCCCCAAGCT
CATCTAGTAGCGAGAGCTCTTCCGTTAGTAGCAGTCATAGCCCCCTCAATAAGGCTCCTGACCCTGGTTC
CTCCCCTCCTCTGAGTAGCCTTTCAGGTCCTAACCCTTTCTGGTTGGCTCTGATCGCCGACCTGGACAAT
CTGGATTCCAGCAGTCCCAGGGTGCCTGGCGAGGAGATCGAGGCTGCACCTCACACACGAGAGGAGGAAG
ATAAGAAGTGCCGGGGA


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG214772 representing NM_001166433
Red=Cloning site Green=Tags(s)

MASSSSPERGLEALRDTDESEGEAPGPSGPRGRGGPSGAGSALRLRSLEAEMAAACVTSTAGEDLGTFSE
PGSQHGDPEGGGGPDLELGHARPMMRSQRELGLTPKGGGKADQGGKGRKGGSGSPPHTKSSRKREQPNPN
RSLMAQGAAGPPLPGARGSPAMPQPESSLSPRPDQSHHFDFPVGNLEAPGPTLRSSTSQGSGSTPVPEAL
RCAESSRAESDQSSPAGRELRQQASPRAPDDDDDGDGGPDPRGSGTPEGWVLRSGVVPFGRRSSASEVSP
EEVRPEAQCTGWNLRPRPRSSASAVSPEARPKAQSAGRNLRPRPRSSASVVSPEARPKAQSAGRNLRPRP
RSSASVVSPEARPEAQSAGRNLRPRATPRVPVAPSSTTRSSSDRGSSRAPRSRSRSRSCSTPRLGSDHQR
SRKIKMRLDLQVDREPESEAEQEEQELESEPGPSSRPQASRSSSRFAVPGRSSLAAEDSPPRRPVRMRAS
SPSPPGRLYPLPKHYFEGVHSPSSSSSESSSVSSSHSPLNKAPDPGSSPPLSSLSGPNPFWLALIADLDN
LDSSSPRVPGEEIEAAPHTREEEDKKCRG

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
Tag TurboGFP
ACCN NM_001166433
ORF Size 1767 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001166433.1, NP_001159905.1
RefSeq Size 2203 bp
RefSeq ORF 1770 bp
Locus ID 102991
Cytogenetics X A1.1

Citations (2)

Other Versions

Other products for "AU022751"
Frequently bought together (2)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

USD 640.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

USD 310.00

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