1600015I10Rik (NM_001081273) Mouse Tagged ORF Clone

CAT#: MR214065

1600015I10Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 1600015I10 gene (1600015I10Rik)

Product Images

Circular map for MR214065

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: 1600015I10Rik
• Synonyms: AI429442; Doxl1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR214065 representing NM_001081273
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTTTGTTCCAGAGCCATGGGGATGGCACAAAAGAGCCTGACACTGGGCTGGGTAACTGCCATCTTGC
  TGTTACAGATGGCCATTGCAGACCGCCCCAGGTGGAGCCTGAATGGCAGGCCCCGGGTATTCGCAGACCT
  GAGTGTTTATGAGATCAAGATTGTGCAGAACTTCCTGATGGACAAAAAGGAGCTGCAGCTGCAGCCATCT
  GAGACACCAACTTTGGGCAAGAACTCAGTATACCTCATAGAAATGCTGCTGCCCGATAAGGAGGATGTGC
  TAGATTTTCTGGACAAAGGAGAAAGGAGTCCTGTGCGGGAAGCCCGTGTCGTCATCTTCTTTGGTGCTCA
  GAAGCATCCTAAAGTCACTGAGTTTGCTGTTGGGCCCATGCCATTACCAATCTACATGCGAGAAATATCT
  CCCGGGCCTGGGCACGATCCATCCTGGGCATCTAGACCCATTTCCAAAGCAGAGTACAGCCTCATCTACC
  GTACGCTGACAGAGGCCACCAAGTCTCTGCATCAGTTCTTCCTTGACACCACTGACTACTCACTAAAAGA
  ACGTGTTGAGGACTATCTGACCTTCACATATATGGCCCCTGATGACACAAAATCTGACCAGCGCTGTAGC
  TGGTTCATGTTGCAACGCTATATGGAAAGCCACTTCCTGCAGCCTACAGGTCTGGAGATCCTTCTGGATC
  ATGGAAGCACAGAAGTCCAGCACTGGAAAGTGAAGCAGCTCTGGTATAACGGCAAGCTCTACAACAGCCC
  AGAGGAACTGGCTCAGAAATACGCAGATGGAGAAGTGAGTATACTGGTACTCACGGAGTCAGTTGACAAG
  GACACAAAGCAGCCCCTAGTCTTCTCTTCCTCCAATATCCCAGAGGACTTTCCCACTACCAACAGCATGG
  CTAGACCCAGAGTGTCTGAGCCCCATGTTTCTAGCTATAACGTAGAGGACAACACGGTGTTCTACAGAGA
  CTGGATTTTCTCCTTCCGGCTAAGACCCTCCTCTGGTCTGCAGATCCTGAATGTGCACTTTCGGGGTGTG
  CGTATTGCATATGAAGTCAGTGTACAAGAGGCGGTGGCACAGTTCGGAGGACATGTATCTGCAGGCACTC
  AGGCCAAGTACATGGATGTGGGCTGGGGCCCTGGCAGTGCTACTCGCCAGTTAGCCGCTGGCATTGATTG
  TCCAGACACAGCCACCTTCCTGGAAGTCATTCATCACTATGACACCGACAGACCAGTCTCCTATCCACGA
  GCTCTCTGTCTCTTTGAGATGCCAGCAGAAATGCCAATTAGGCACCACTTTAACTCCAACTTTAGAGAAG
  ACTTCAACTCCAGTGCAGGGACAAATGGACACATGCTGGTGCTACGGACAATCTCATCAGTCCTCCACTA
  TGATTATATTTGGGACTTTGTTTTCTACACTAATGGAGCAATGAAGGCAAAAATGCAAGCCACTGGCTTG
  GTCCATGCCACCTACTACCAGCCTGAGGAGATGCGCTACATCTCTCAATCACACACCCATGTATTCAGCA
  ACATTCACACCCACCTAGTACATTACCGCATTGACCTGGATGTAGCAGGCACCAAGAACAGATTCCAGAC
  ACTGCAGATCATTCGGAAAAACATCACCAACTCTAAGAACCTGAGACATCAGGCACTTGGAAATACCCTA
  AAGCAAGCAAATTACACCCAAGAGCAACAAGCAGCCTTCTACTTTGGAAAGCCGCTTCCCAATTACCTAA
  TCTTTAGCAACCTCCATACGACCCACGGGATGGGCCACAGGAGCAGGTACCGTCTGAAGACCTACTCCAA
  AATCCATCAGGTGCCACTGCCAGGCTGGCAAAAGAAGCAAGGTGTCTCTTGGACCAGGTATCCCTTGGCT
  GTGACCGAGTACCAAGATTCTGAGAAGTGCAGCAGCAGCATCTACAGCCAAAATAATCCTTGGGATCCCC
  CTGTGGTCTTCGAGGATTTTATTCAGAACAATACAAACATTGAAGATAAGGATTTGGTGGCCTGGGCCAC
  AGTGGGCTTCCACAACATGCCCCGTTCTGAGGCTAGTACCAACATGACCACACCTGAGAACTCTGTGGGT
  TTTGTGCTCCGACCCTTCAACCTCCCTGGCTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR214065 representing NM_001081273
  Red=Cloning site Green=Tags(s)
  
  MLCSRAMGMAQKSLTLGWVTAILLLQMAIADRPRWSLNGRPRVFADLSVYEIKIVQNFLMDKKELQLQPS
  ETPTLGKNSVYLIEMLLPDKEDVLDFLDKGERSPVREARVVIFFGAQKHPKVTEFAVGPMPLPIYMREIS
  PGPGHDPSWASRPISKAEYSLIYRTLTEATKSLHQFFLDTTDYSLKERVEDYLTFTYMAPDDTKSDQRCS
  WFMLQRYMESHFLQPTGLEILLDHGSTEVQHWKVKQLWYNGKLYNSPEELAQKYADGEVSILVLTESVDK
  DTKQPLVFSSSNIPEDFPTTNSMARPRVSEPHVSSYNVEDNTVFYRDWIFSFRLRPSSGLQILNVHFRGV
  RIAYEVSVQEAVAQFGGHVSAGTQAKYMDVGWGPGSATRQLAAGIDCPDTATFLEVIHHYDTDRPVSYPR
  ALCLFEMPAEMPIRHHFNSNFREDFNSSAGTNGHMLVLRTISSVLHYDYIWDFVFYTNGAMKAKMQATGL
  VHATYYQPEEMRYISQSHTHVFSNIHTHLVHYRIDLDVAGTKNRFQTLQIIRKNITNSKNLRHQALGNTL
  KQANYTQEQQAAFYFGKPLPNYLIFSNLHTTHGMGHRSRYRLKTYSKIHQVPLPGWQKKQGVSWTRYPLA
  VTEYQDSEKCSSSIYSQNNPWDPPVVFEDFIQNNTNIEDKDLVAWATVGFHNMPRSEASTNMTTPENSVG
  FVLRPFNLPGF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001081273
• ORF Size: 2133 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001081273.2, NP_001074742.2
• RefSeq Size: 2493 bp
• RefSeq ORF: 2136 bp
• Locus ID: 69761
• Cytogenetics: 6 B2.3
• MW: 81.5 kDa