D130040H23Rik (NM_172491) Mouse Tagged ORF Clone

CAT#: MR213717

D130040H23Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA D130040H23 gene (D130040H23Rik)

Product Images

Circular map for MR213717

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: D130040H23Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR213717 representing NM_172491
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATATTGAAGAACATTTTTGTCAAAATTCTAGAATACATAGAAGGCATGAAAGAGGTCCTGGAGAGAA
  ACCCTATGAATATACTCAGTGTGGTAAAGCCTTTGCACAACACAGTCATCTTCAAGGGCATGAAAGAATT
  CTTACTGGAAAGAAACCCTATGAATGTAATCAATGTGGTAAAGCCTTTATATGTCACAGTAGTCTTTCAA
  ATCATGAAAAGCATCATACTGGAGAGAAACCATACAAATGTATTGAATGTGGTAAAGCCTTTTCACAACA
  GAGTCACCTCCAGATACATAAAAGAATACATACTGGAGAGAAGCCCTATGAATGTAATCAGTGTGCTAAA
  GCCTTTTCACAACAGAGCCATCTCCAGAAACATAAAAGAACACATACTGGAGAGAAGCCCTACAAATGTA
  ATCAATGTAATAAAGACTTTTCACAACAAAGTCATCTCCAAATCCATAGAAGAACACATACTGGAGAGAA
  ACCCTACAAATGTAATCAATGTGATAAAGTCTTTTCACAACAAACTCATCTCCAAAGACATAAAAGAACA
  CATACTGGAGAGAAGCCCTACAAATGTAATCAATGTGGTAAAGCCTTTGCTCATCACAGTTATCTTGAAA
  AACATCAAAGAACACACACTGGAGAGAAACCTTATCAATGCAACCAATGTGGTAAAGCCTTTGCACATCA
  CAGTTATCTCCAAAAACATAAAAGAACACACACTGGAGAGAAACCTTATCAATGCAATCAATGTGGTAAA
  ACTTTTGCATGTCACAGAAGTCTTCAAATTCATGAAAGAATTCATACTGGAGAGAAACCTTATGAATGTT
  GTCAATGTGGTAAGACCTTTGCACATCACAATTATCTCCGAAACCATAAAAGAACACATACTGGAGAGAA
  CCCCTACAAATGTAAGGAATGCGGTAAAGCCTTTGCATGTCACAGTCATCTTCAAAGGCATGAAAGAGTT
  CATACTGGAGAGAAACCCTATGAGTGTAGTCAATGTGGTAAAGCCTTTGCATGTCACAGCAGTCTTCAAA
  ATCATGAAAAAAATCATATTGAAGAGAAACTCTATGAATTATGTAAGCAATATGGTAACGCCTTTGTACA
  TCACAGTTATCTCTGAAAACATAAAACACAAAAGAATATATTCTACAGAATATAATCAGGATTTTAAAGT
  CATGTCACAGTTGTCTTTGAGGACA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR213717 representing NM_172491
  Red=Cloning site Green=Tags(s)
  
  MILKNIFVKILEYIEGMKEVLERNPMNILSVVKPLHNTVIFKGMKEFLLERNPMNVINVVKPLYVTVVFQ
  IMKSIILERNHTNVLNVVKPFHNRVTSRYIKEYILERSPMNVISVLKPFHNRAISRNIKEHILERSPTNV
  INVIKTFHNKVISKSIEEHILERNPTNVINVIKSFHNKLISKDIKEHILERSPTNVINVVKPLLITVILK
  NIKEHTLERNLINATNVVKPLHITVISKNIKEHTLERNLINAINVVKLLHVTEVFKFMKEFILERNLMNV
  VNVVRPLHITIISETIKEHILERTPTNVRNAVKPLHVTVIFKGMKEFILERNPMSVVNVVKPLHVTAVFK
  IMKKIILKRNSMNYVSNMVTPLYITVISENIKHKRIYSTEYNQDFKVMSQLSLRT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_172491
• ORF Size: 1215 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172491.3
• RefSeq Size: 2002 bp
• RefSeq ORF: 1218 bp
• Locus ID: 211135
• Cytogenetics: 8 B3.3
• MW: 47.3 kDa