Cfap157 (NM_025619) Mouse Tagged ORF Clone

CAT#: MR212962

1700019L03Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 1700019L03 gene (1700019L03Rik)

Product Images

Circular map for MR212962

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cfap157
• Synonyms: 1700019L03Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR212962 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCCAAAAAAGAAGCCAAACAAAGGAGGCAAGGAGATGCAGGGCAAGAAGATAGGGGGCAAGAAGG
  ATGCCAGTGGGACCAAGACTCCAGAGTTGGCCATGGTGGAGGAGCTGAAGGAATTCTACCACAAACAGAT
  CCAAGACCTGGAGGACAGGCTGGCCCGGTATCAGCGGAAATGGGATGAACTGGCTGTGCGGGAGAAGCTG
  TTCCATCAGGAGTTCGAGCAGCTGGCCAACAACAAGAAGGAGATTGTGGCCTTCCTCAAGCGCACGCTGA
  ACCAGAAGGTGGATGAGATCACAGACCTTAATGACCAGCTGCAGAGCCTGGAGGTGGCCAAGGATATGGA
  GAAGGACGCGTTCGAGGCCCAACTGGCCCAGGTGCGCCATGAATTCCAGGAAATCAAGGACCAGCTCACC
  ACAGAGAACATCGCCCTCGGGATCAAGCTGAGTTCCCTGGAAGAATTCCGGCTGCAGAAAGAGGAGCTCA
  CGGAGAAGTACTTGGCACTGGAGGAGCAGCTCCATAGGCAGGAGGGCGAGTACAAGGAATACGTGTACAA
  CCTGGAGAAGAAGTCTGTGCTGGACAAGGACAGGCTGAGGAAAGAGATTATCCAGCGTGTGAACCTGGTG
  GCCACAGAGTTCCGAAAGATGGCCACCAACCAGATGTGGGAGACGACCAGGCGGGCCATCCTAGAGAACA
  ACAGCGTGACTCTGCAGCTAAACAAGGTGTCCAAGCATGGTGTGCAGCTGCTGCAGGAGAATGAGCAGCT
  CAAGGGCACCCAGAACAAGCTGTGCCAACAGCTGGAGATGCTGGAGAACACCCAAGAGATCATGGCCAGA
  AAGAACATAGGGCATAAGAAGATTATCCTCATGCTGACCGAGAAGTGCCGCCAACAGAGAAAGGGCACAG
  AGGAGACGGAGCAACTGCGCCTCCTGCTGTCCCAACTGGAGCAGAACTTCCAGAATGTGCAGAGGGACAA
  CCAGACCCTGAGGAGTGAGAAAGACCAGCTGGAAGCGCAACTGAAGGAGAAGCAGGCAGAGGCAAATCGG
  CTACAGGAGGAGCTGACCAAGGAACAGAAAATTCGGGCAAACCTTAAAACAGTCCTGATCCAGGCGACCT
  CCATGCTACAGGACATTGTGCACATGCAAACAGAGGCGGAGGACGGCGACTTTGATGTGGTGTTCCAACT
  GCAGCGCAAGGAGCTGCTGCAGCAACTGCTGGTATTGCTCAGCTCAGGGGTGGTTTTGAAAACACAGCCG
  GACATGGGTTCCCACCAGGACAAGCAGCCCCAGGGCCTATCCAAAGAAAGCCAACGCATCACCCAGACAT
  CCAAGGAAGGGGCTGTTTCTCTGCTGCAGCAGCTCTCGACCATCACAACCTATAAGCCAGGGGATCTTGG
  GCTGGTGCCTCGACGTCCGGTCCGCATCCCACTCAAACCCCAGGATCTCAGGCCCCTCTCCTATATCACC
  CGAATGGGGATCTGCCAGAACACGAATGAGATCTACCCATCTGGAGCTCTCAAAAGGTTCAGAAAGTTTA
  CCCTTCCTAGACCTTTCCTACACCGCAAA
  
  ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGA
  TTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR212962 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAPKKKPNKGGKEMQGKKIGGKKDASGTKTPELAMVEELKEFYHKQIQDLEDRLARYQRKWDELAVREKL
  FHQEFEQLANNKKEIVAFLKRTLNQKVDEITDLNDQLQSLEVAKDMEKDAFEAQLAQVRHEFQEIKDQLT
  TENIALGIKLSSLEEFRLQKEELTEKYLALEEQLHRQEGEYKEYVYNLEKKSVLDKDRLRKEIIQRVNLV
  ATEFRKMATNQMWETTRRAILENNSVTLQLNKVSKHGVQLLQENEQLKGTQNKLCQQLEMLENTQEIMAR
  KNIGHKKIILMLTEKCRQQRKGTEETEQLRLLLSQLEQNFQNVQRDNQTLRSEKDQLEAQLKEKQAEANR
  LQEELTKEQKIRANLKTVLIQATSMLQDIVHMQTEAEDGDFDVVFQLQRKELLQQLLVLLSSGVVLKTQP
  DMGSHQDKQPQGLSKESQRITQTSKEGAVSLLQQLSTITTYKPGDLGLVPRRPVRIPLKPQDLRPLSYIT
  RMGICQNTNEIYPSGALKRFRKFTLPRPFLHRK
  
  TRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-NotI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_025619
• ORF Size: 1572 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_025619.1, NP_079895.1
• RefSeq Size: 1675 bp
• RefSeq ORF: 1572 bp
• Locus ID: 227736
• UniProt ID: Q0VFX2
• Cytogenetics: 2 B
• MW: 61 kDa
• Gene Summary: Specifically required during spermatogenesis for flagellum morphogenesis and sperm motility (PubMed:27965440). May be required to suppress the formation of supernumerary axonemes and ensure a correct ultrastructure (PubMed:27965440).[UniProtKB/Swiss-Prot Function]