Cfap157 (NM_025619) Mouse Untagged Clone

CAT#: MC217514

1700019L03Rik (untagged) - Mouse RIKEN cDNA 1700019L03 gene (1700019L03Rik), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Cfap157
• Synonyms: 1700019L03Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC217514 representing NM_025619
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCCAAAAAAGAAGCCAAACAAAGGAGGCAAGGAGATGCAGGGCAAGAAGATAGGGGGCAAGAAGG
  ATGCCAGTGGGACCAAGACTCCAGAGTTGGCCATGGTGGAGGAGCTGAAGGAATTCTACCACAAACAGAT
  CCAAGACCTGGAGGACAGGCTGGCCCGGTATCAGCGGAAATGGGATGAACTGGCTGTGCGGGAGAAGCTG
  TTCCATCAGGAGTTCGAGCAGCTGGCCAACAACAAGAAGGAGATTGTGGCCTTCCTCAAGCGCACGCTGA
  ACCAGAAGGTGGATGAGATCACAGACCTTAATGACCAGCTGCAGAGCCTGGAGGTGGCCAAGGATATGGA
  GAAGGACGCGTTCGAGGCCCAACTGGCCCAGGTGCGCCATGAATTCCAGGAAATCAAGGACCAGCTCACC
  ACAGAGAACATCGCCCTCGGGATCAAGCTGAGTTCCCTGGAAGAATTCCGGCTGCAGAAAGAGGAGCTCA
  CGGAGAAGTACTTGGCACTGGAGGAGCAGCTCCATAGGCAGGAGGGCGAGTACAAGGAATACGTGTACAA
  CCTGGAGAAGAAGTCTGTGCTGGACAAGGACAGGCTGAGGAAAGAGATTATCCAGCGTGTGAACCTGGTG
  GCCACAGAGTTCCGAAAGATGGCCACCAACCAGATGTGGGAGACGACCAGGCGGGCCATCCTAGAGAACA
  ACAGCGTGACTCTGCAGCTAAACAAGGTGTCCAAGCATGGTGTGCAGCTGCTGCAGGAGAATGAGCAGCT
  CAAGGGCACCCAGAACAAGCTGTGCCAACAGCTGGAGATGCTGGAGAACACCCAAGAGATCATGGCCAGA
  AAGAACATAGGGCATAAGAAGATTATCCTCATGCTGACCGAGAAGTGCCGCCAACAGAGAAAGGGCACAG
  AGGAGACGGAGCAACTGCGCCTCCTGCTGTCCCAACTGGAGCAGAACTTCCAGAATGTGCAGAGGGACAA
  CCAGACCCTGAGGAGTGAGAAAGACCAGCTGGAAGCGCAACTGAAGGAGAAGCAGGCAGAGGCAAATCGG
  CTACAGGAGGAGCTGACCAAGGAACAGAAAATTCGGGCAAACCTTAAAACAGTCCTGATCCAGGCGACCT
  CCATGCTACAGGACATTGTGCACATGCAAACAGAGGCGGAGGACGGCGACTTTGATGTGGTGTTCCAACT
  GCAGCGCAAGGAGCTGCTGCAGCAACTGCTGGTATTGCTCAGCTCAGGGGTGGTTTTGAAAACACAGCCG
  GACATGGGTTCCCACCAGGACAAGCAGCCCCAGGGCCTATCCAAAGAAAGCCAACGCATCACCCAGACAT
  CCAAGGAAGGGGCTGTTTCTCTGCTGCAGCAGCTCTCGACCATCACAACCTATAAGCCAGGGGATCTTGG
  GCTGGTGCCTCGACGTCCGGTCCGCATCCCACTCAAACCCCAGGATCTCAGGCCCCTCTCCTATATCACC
  CGAATGGGGATCTGCCAGAACACGAATGAGATCTACCCATCTGGAGCTCTCAAAAGGTTCAGAAAGTTTA
  CCCTTCCTAGACCTTTCCTACACCGCAAATAG
  
  ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGA
  TTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-NotI
• ACCN: NM_025619
• Insert Size: 1572 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_025619.1, NP_079895.1
• RefSeq Size: 1675 bp
• RefSeq ORF: 1572 bp
• Locus ID: 227736
• UniProt ID: Q0VFX2
• Cytogenetics: 2 B
• Gene Summary: Specifically required during spermatogenesis for flagellum morphogenesis and sperm motility (PubMed:27965440). May be required to suppress the formation of supernumerary axonemes and ensure a correct ultrastructure (PubMed:27965440).[UniProtKB/Swiss-Prot Function]