Cox14 (NM_183256) Mouse Tagged ORF Clone

CAT#: MR212724

Cox14 (Myc-DDK-tagged) - Mouse RIKEN cDNA 2310016M24 gene (2310016M24Rik)

Product Images

Circular map for MR212724

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cox14
• Synonyms: 2310016M24Rik; AA959804
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR212724 representing NM_183256
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCATCTGCCAAGCAGCTAGCCGATATCGGCTACAAGACCTTCTCTGCCTCGATGATGCTCCTCACTG
  TGTATGGGGGTTACCTCTGCAGTGTACGAGCCTACCGTTACCTCCAGCTGCGCAGTGCCAGGCGCCAGGC
  TGCAGAAGAGCAGAAGACCTCAGGAGTCCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR212724 representing NM_183256
  Red=Cloning site Green=Tags(s)
  
  MPSAKQLADIGYKTFSASMMLLTVYGGYLCSVRAYRYLQLRSARRQAAEEQKTSGVL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_183256
• ORF Size: 171 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_183256.3, NP_899079.1
• RefSeq Size: 741 bp
• RefSeq ORF: 174 bp
• Locus ID: 66379
• UniProt ID: Q8BH51
• Cytogenetics: 15 F1
• MW: 6.8 kDa
• Gene Summary: Core component of the MITRAC (mitochondrial translation regulation assembly intermediate of cytochrome c oxidase complex) complex, that regulates cytochrome c oxidase assembly. Requires for coordination of the early steps of cytochrome c oxidase assembly with the synthesis of MT-CO1.[UniProtKB/Swiss-Prot Function]