1700017B05Rik (NM_028820) Mouse Tagged ORF Clone

CAT#: MR212450

1700017B05Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 1700017B05 gene (1700017B05Rik)

Product Images

Circular map for MR212450

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: 1700017B05Rik
• Synonyms: AA682102; D9Ertd278e
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR212450 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGAGAAGCGGCCCCTAGGGCCTCTGGGGCCTATGATGTATGGCAAGCTGCCTCGCCTGGAGCCAG
  ACCCAGGGCCAGGACACAGCCTGCCCCTTTCTGCTAGCAGCCAGGACTCCTGCAATTACAAAGGTGCATA
  TTTCTCCTGCCCCATAGGGGGTACTTCGAAGGCAGGATCTGAGCGGTTGGCATCCTGGACCCCATATCCA
  TCTTTGTACCCCACTGGTGTGGCAGGATCCCCACTCCGGGGTGACAACCTACTGACAAACTGCCTGCTCT
  ACCGTCCCCCGACAGAAGGCTCTGAAAAGATACAAGACTCAAGTGAGCTGCTGCCCTTCGGTCCCCAGGC
  TCATGCGTACCCAGGCCCGCCCCTGGCCGCGCCCAAGCCTGTCTACCGCAACCCTTTGTGTTACGGACTC
  TCCACTTGCCTCGGAGATGGGGGAACAAAGAGATCATTGGATGGTGACTGGACCCTGGTGACAGGGCCCC
  TGCTGCCTTCTGCTGACCCACCCTGTCCTCTAGCTACAGCTCCTGGCAAGGGCCAGCCCCTGGATGGGAC
  GTTCTTGCGAGGACTACCATCTGGGGGACCTGGCAAGGACTCTTCCCTGCCTTTTTCCCCGTGCCAGGCA
  TTCTTGGAGAAGTATCGAACTATCCAAAGCACAGGTTTCCTGGCTTCCAAGTACACCAGTCCTTATCCTG
  GGGATGCAAAGCAAGCAATGTCTGAGGGGCCCTCCAGTCCTTGGACTCAACTTGCACAGCCCCTAGGGCC
  ACCTTGCCAGGATGCAGTGGCAGCCCACTATCCACTGCCCCCACCGCCACAGGCCCTGCCTTGCCCTCCA
  TCTTGTCATCCAGAAAAGCAGGGAAGCTATGGCTCGTTGCTCCCACTGCCACCTCTAGGAGCCCACAAGG
  GGGCTGCGTACCAAGCTGGGGGGCTGGGCAGCCCTTACCTGAGGCAACAGGCACCCCAGGCTCCGTACAT
  GCCCCCTCTTGGGATAGACAGTTATTCCTACTCCTCTGCACCCCTCCCAGCACCATCACCAGGGCTCAAA
  CTGGAGCCCCCTCTTGCTCCAAGGTGCCCACTGGACTTTGTTCCCCAAACTCTGGGTTTTCCTTATGCCA
  GGGATGACCTCTCTCTCTATGGGGCATCCCCGGGGCTTGGGGGAACACCGCCTTCCCATTCCCAGAACAG
  TGTGCAGCCTGTGCCACAGCCCGGTGCCTTCCAGCGAGCATGCCAGCCTTTGCCAGCCAGCCAACCCTGC
  TCTGAGCCTGTGAGACCTGCAGAGAAGCCAACTCCAGAAGCTCAAGAGAAGATGTGGCTGCCCAGCTGCA
  GGAAAGAGCAACTCCAGCCCAGGCCAAACGAGCGTCCTGGAGTGCCTATTGTTATCCGGGACAGTCCAGT
  TCCCCGCACCTCACCAGCACTGCACCCCTGTGCCAAGGAGCGGCAGTCTGTCCCACAGAAGGATGCCAGG
  CCTCCCAGCTCCCCACCAATGCCCGTGATTGACAACGTTTTTAGTCTGGCCCCTTACCGTGACTACCTGG
  ATGTGCAGACACCAGAGCCCAGGGCTGAGCGGGACTCGGCTCCAGCCACCAGTAAGAGCCAAGACAAAGA
  TTGCAAGGGGAACCTGCCTGCTCAGGATGGAGCCTCCAGGTCTCACTGCTCTCTTCGTGAGGAGGTGGCT
  CTGGACTTGAGTGTTAAAAAGACGATGGCAGAGGGTGTCCCTGTCAAGGTCCCCAGTCCTGAGGTGCATG
  AAAAGCCCGCTGAAGCTGTGGATGGGCCAGGTATAGAAAACACAGTGTCAGGTCTGCCTGGCCTAAAAAA
  GATGGTCACAGAAATCCCTGAAGTAACTGCAGAAGCCACACCAAGGACCAACTTCCATAGCTCTGTGGCC
  TTCATGTTCCGAAAATTCAAGATACTCCGTACTGCGCCCGTACCTGCAGCTATTGTCTCATCTCCCACTA
  CCCCGGCTCCTGTCCCTGCGCCTGCCCCTGCACAGCCTGTACCCAACCCCCCGTCTGTGCCAGTGGGACT
  GCAGATCCTCACTCAGCCTTTGCCAGTGGCATGCTTCAACCTGGCCCTGCCTAGCCCACCAGCCATAGTG
  GCCTCCCCGGCCCCAGCCTCTGCTCCACCTCCATCACCAGCTCCTGCTCCTGCTCCAGCCTCGGGTCCTG
  CTCCATCGTCTGCTCAAGTTCCCACTGCAGCCCCAGTGGACTCCCCGGAACAGCACTTCACAGGGCTGCA
  CACATCCCTCTGTGATGCCATCTCAGGCTCTGTGGCCCATTCTCCACCAGAGAAACTGCGGGAGTGGCTT
  GAGACAACTGGGCCGTGGGGCCAGGCTGCGTGGCAGGACTGCCAGGGTGTTCAGGGGCTACTAGGCAAAC
  TGCTGTCCCAGCTGCAGAAGTTCGTGTGCACACAGCGGTGCCCCTTCCCTCATGTAGTACGGGCTGGTGC
  CATCTTTGTGCCCATCCATCTGGTGAAGGAGCGTCTCTTTCCAAAGCTGCCACCGGCCTCTGTGGACCAC
  GTGTTGCAGGACCACCGAGTGGAACTACGGCCCACCACTCTGTCGGAGGAGCGTGCGCTGCGTGAACGTG
  CCCTGCACGGCTGCACCTCGCGCATGCTGAAGCTGCTGGCACTGCGTCAGCTGCCTGACATCTATCCTGA
  CCTGCTGGGCCTACAGTGGCATGACTGTGTCCGCCGCCAGCTGGGTGGCTTTGACACTGAGGCCAGAACT
  TTATCCCCATCAGAACCCACGGTCACCAGAGATGAGCCAGAAAGCCAAGCCTTGGCTGGAAAGTTGCCAG
  CCCCCAAGGTCAAGAAGCCAGGGAGGAAGCCACCGACGCCTGGCCCTGAGAAAGCAGAGGCAGCCACTGG
  GGAAGGGTCACGTAATCCCTCACCTAGCTCTGGTGCCAGCACCAGCCCACCAGGTCCCACCCTGAGGGCC
  CGCTTCCGCAACCTGTTAGAAAATGCCTGGCTCAATGGTGTGGCACTGCCTACTTGGGGTCACAAGGCCT
  CCGGAGCAGATCGATCTTCGCCTCACCCCCAGCTCTTGGGCAGCCAGACCCATCATCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR212450 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAEKRPLGPLGPMMYGKLPRLEPDPGPGHSLPLSASSQDSCNYKGAYFSCPIGGTSKAGSERLASWTPYP
  SLYPTGVAGSPLRGDNLLTNCLLYRPPTEGSEKIQDSSELLPFGPQAHAYPGPPLAAPKPVYRNPLCYGL
  STCLGDGGTKRSLDGDWTLVTGPLLPSADPPCPLATAPGKGQPLDGTFLRGLPSGGPGKDSSLPFSPCQA
  FLEKYRTIQSTGFLASKYTSPYPGDAKQAMSEGPSSPWTQLAQPLGPPCQDAVAAHYPLPPPPQALPCPP
  SCHPEKQGSYGSLLPLPPLGAHKGAAYQAGGLGSPYLRQQAPQAPYMPPLGIDSYSYSSAPLPAPSPGLK
  LEPPLAPRCPLDFVPQTLGFPYARDDLSLYGASPGLGGTPPSHSQNSVQPVPQPGAFQRACQPLPASQPC
  SEPVRPAEKPTPEAQEKMWLPSCRKEQLQPRPNERPGVPIVIRDSPVPRTSPALHPCAKERQSVPQKDAR
  PPSSPPMPVIDNVFSLAPYRDYLDVQTPEPRAERDSAPATSKSQDKDCKGNLPAQDGASRSHCSLREEVA
  LDLSVKKTMAEGVPVKVPSPEVHEKPAEAVDGPGIENTVSGLPGLKKMVTEIPEVTAEATPRTNFHSSVA
  FMFRKFKILRTAPVPAAIVSSPTTPAPVPAPAPAQPVPNPPSVPVGLQILTQPLPVACFNLALPSPPAIV
  ASPAPASAPPPSPAPAPAPASGPAPSSAQVPTAAPVDSPEQHFTGLHTSLCDAISGSVAHSPPEKLREWL
  ETTGPWGQAAWQDCQGVQGLLGKLLSQLQKFVCTQRCPFPHVVRAGAIFVPIHLVKERLFPKLPPASVDH
  VLQDHRVELRPTTLSEERALRERALHGCTSRMLKLLALRQLPDIYPDLLGLQWHDCVRRQLGGFDTEART
  LSPSEPTVTRDEPESQALAGKLPAPKVKKPGRKPPTPGPEKAEAATGEGSRNPSPSSGASTSPPGPTLRA
  RFRNLLENAWLNGVALPTWGHKASGADRSSPHPQLLGSQTHHL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_028820
• ORF Size: 3072 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_028820.2, NP_083096.1
• RefSeq Size: 4934 bp
• RefSeq ORF: 3072 bp
• Locus ID: 74211
• UniProt ID: Q3TEI4
• Cytogenetics: 9 30.89 cM
• MW: 108.2 kDa