Cacna1g (NM_001177890) Mouse Tagged ORF Clone

CAT#: MR212118

Cacna1g (Myc-DDK-tagged) - Mouse calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), transcript variant 4

Product Images

Circular map for MR212118

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cacna1g
• Synonyms: a1G; alpha-1G; Cav3.1d; mKIAA1123; [a]1G
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR212118 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGAGGAAGAGGATGGAGCGGGCGCCGAGGAGTCGGGACAGCCCCGGAGCTTCACGCAGCTCAACG
  ACCTGTCTGGGGCCGGGGGCCGGCAGGGGCCGGGGTCGACGGAAAAGGACCCGGGCAGCGCGGACTCCGA
  GGCGGAGGGGCTGCCCTACCCGGCGCTGGCCCCGGTGGTTTTCTTCTACTTGAGCCAGGACAGCCGCCCG
  CGGAGCTGGTGTCTCCGCACGGTCTGTAACCCGTGGTTCGAGCGAGTCAGCATGCTGGTTATTCTCCTCA
  ACTGTGTGACTCTGGGTATGTTCAGGCCGTGTGAGGACATTGCCTGTGACTCCCAACGCTGCCGGATCCT
  GCAGGCCTTCGACGACTTCATCTTTGCCTTCTTTGCTGTGGAAATGGTGGTGAAGATGGTCGCTTTGGGT
  ATCTTTGGGAAGAAATGTTACCTGGGAGACACTTGGAACCGGCTTGACTTTTTTATCGTCATTGCTGGGA
  TGCTGGAGTACTCGCTGGACCTGCAGAATGTCAGCTTCTCCGCAGTCAGGACAGTCCGTGTGCTGCGACC
  GCTCAGGGCCATTAACCGGGTGCCCAGCATGCGCATTCTCGTCACATTACTGCTGGATACCTTGCCTATG
  CTGGGCAATGTCCTGCTGCTCTGTTTCTTCGTCTTTTTCATCTTTGGCATCGTTGGTGTTCAGCTGTGGG
  CAGGGCTACTTCGAAACCGATGCTTCCTCCCTGAGAATTTCAGCCTCCCGCTGAGTGTGGACTTGGAGCC
  TTACTACCAGACAGAGAATGAGGACGAGAGCCCCTTCATCTGCTCTCAGCCTCGGGAGAACGGCATGCGA
  TCCTGCAGGAGCGTGCCCACACTGCGCGGGGAAGGCGGTGGTGGTCCACCCTGCGGTCTGGACTATGAGG
  CCTATAACAGTTCCAGCAATACCACCTGTGTCAACTGGAACCAATACTATACCAACTGCTCTGCAGGCGA
  GCACAACCCCTTCAAAGGCGCCATCAACTTTGACAACATTGGCTATGCCTGGATCGCCATCTTCCAGGTC
  ATCACACTGGAGGGCTGGGTCGACATCATGTACTTTGTGATGGATGCTCATTCCTTCTACAACTTCATCT
  ACTTCATTCTTCTCATCATCTTCTCTGAGACCAAGCAACGGGAGAGTCAGCTGATGCGGGAGCAGCGTGT
  ACGATTCCTGTCCAATGCTAGCACCCTGGCAAGCTTCTCTGAGCCAGGCAGCTGCTATGAGGAGCTTCTC
  AAGTACCTGGTGTACATCCTCCGCAAAGCAGCCCGCAGGCTGGCCCAGGTCTCTAGGGCTGTAGGCGTGC
  GGGCTGGGTTGCTCAGCAGCCCAGTGGCCCGTGGTGGGCAGGAGCCCCAGCCCAGTGGCAGCTGCTCTCG
  TTCACACCGTCGTCTGTCTGTCCACCACCTGGTCCACCACCATCACCACCACCATCACCACTACCACCTG
  GGTAATGGGACGCTCAGAGTTCCCCGGGCCAGCCCAGAGATCCAGGACAGGGATGCCAATGGGTCCCGCT
  GGCTCATGCTGCCACCACCCTCTACCCCCACTCCCTCTGGGGGCCCTCCGAGGGGTGCGGAGTCTGTACA
  CAGCTTCTACCATGCTGACTGCCACTTGGAGCCAGTCCGTTGCCAGGCGCCCCCTCCCAGGTCCCCATCG
  GAGGCATCTGGCAGGACTGTGGGTAGTGGGAAGGTATACCCCACTGTGCATACCAGCCCTCCACCAGAGA
  TGCTGAAGGATAAGGCACTAGTGGAGGTGGCCCCCAGCCCTGGGCCCCCCACCCTCACCAGCTTCAACAT
  CCCACCTGGGCCCTTCAGCTCCATGCACAAGCTCCTGGAGACACAGAGTACGGGAGCCTGCCATAGCTCC
  TGCAAAATCTCCAGCCCTTGCTCCAAGGCAGACAGTGGAGCCTGTGGGCCGGACAGTTGTCCCTACTGTG
  CCCGGACAGGAGCAGGGGAGCCAGAGTCCGCTGACCATGAAATGCCTGACTCAGACAGTGAGGCTGTGTA
  TGAGTTCACACAGGACGCTCAGCACAGCGACCTCCGGGATCCCCACAGACGGCGACGGCCGAGCCTGGGC
  CCAGATGCAGAGCCTAGTTCTGTGCTGGCCTTCTGGAGGCTGATCTGCGACACATTCCGGAAGATCGTAG
  ATAGCAAATACTTTGGCCGGGGAATCATGATCGCTATCCTGGTCAATACCCTCAGCATGGGCATCGAGTA
  CCACGAGCAGCCCGAGGAGCTCACCAACGCCCTGGAAATCAGCAACATCGTCTTCACCAGCCTCTTCGCC
  TTGGAGATGCTGCTGAAGCTGCTCGTCTACGGTCCCTTCGGCTACATTAAGAATCCCTACAACATCTTTG
  ATGGCGTCATTGTGGTCATCAGCGTGTGGGAGATTGTGGGCCAGCAGGGAGGTGGCCTGTCGGTGCTGCG
  GACCTTCCGCCTGATGCGGGTGCTGAAGCTAGTTCGCTTCCTGCCGGCACTGCAGCGGCAGCTCGTGGTG
  CTCATGAAGACCATGGACAACGTGGCCACCTTCTGCATGCTGCTCATGCTCTTCATCTTCATCTTCAGCA
  TCCTGGGCATGCACCTTTTTGGTTGCAAGTTCGCATCTGAACGGGATGGGGACACGTTGCCAGACCGGAA
  GAATTTTGACTCCCTGCTCTGGGCCATTGTCACTGTCTTTCAGATTCTGACTCAGGAAGACTGGAATAAA
  GTCCTTTACAACGGCATGGCCTCCACGTCATCTTGGGCTGCTCTTTACTTCATCGCCCTCATGACTTTTG
  GCAACTACGTGCTCTTTAACCTGCTTGTCGCCATTCTCGTGGAGGGTTTCCAGGCAGAGGGAGATGCCAC
  CAAGTCTGAGTCAGAGCCTGATTTCTTTTCGCCCAGTGTGGATGGTGATGGGGACAGGAAGAAGCGCTTG
  GCCCTGGTGGCCTTGGGAGAACACTCGGAACTACGAAAGAGCCTTTTGCCACCTCTCATCATCCACACAG
  CTGCTACACCGATGTCACTGCCCAAGAGCTCCAGCACAGGTGTGGGGGAAGCACTGGGCTCTGGCTCTCG
  CCGCACCAGTAGCAGTGGGTCCGCTGAGCCTGGAACTGCCCATCATGAGATGAAATCACCGCCAAGTGCC
  CGAAGCTCCCCGCACAGTCCCTGGAGCGCAGCAAGCAGCTGGACCAGCAGGCGCTCCAGCCGGAACAGCC
  TGGGCCGGGCCCCCAGCCTGAAGCGTAGGAGCCCAAGCGGGGAGCGGAGGTCCCTGCTGTCTGGAGAGGG
  TCAGGAGAGCCAGGATGAGGAGGAGAGTTCAGAAGAGGACCGGGCCAGCCCAGCAGGCAGTGATCATCGC
  CACAGGGGTTCCTTGGAACGTGAGGCCAAGAGTTCCTTTGACCTGCCTGACACCCTGCAGGTGCCCGGGC
  TTCATCGAACAGCCAGCGGTCGGAGCTCTGCCTCTGAACACCAAGACTGTAATGGCAAGTCGGCTTCAGG
  GCGTTTGGCCCGCACCCTGCGGGCTGATGACCCCCCACTGGATGGGGATGATGGCGATGATGAGGGCAAC
  CTGAGCAAAGGGGAACGCTTACGAGCCTGGGTCCGAGCCCGGCTCCCTGCCTGTTGCCGAGAGCGAGATT
  CCTGGTCTGCCTATATCTTCCCTCCCCAGTCAAGGTTTCGTCTCCTGTGTCACCGGATCATCACCCACAA
  GATGTTTGACCATGTGGTCCTCGTCATCATCTTCCTCAACTGTATCACCATCGCTATGGAGCGCCCCAAA
  ATTGACCCCCACAGCGCTGAACGCATCTTCCTGACCCTCTCCAACTACATCTTCACGGCAGTCTTCCTGG
  CTGAAATGACAGTGAAGGTGGTGGCACTGGGCTGGTGCTTTGGGGAGCAGGCCTACCTGCGCAGCAGCTG
  GAACGTGCTGGACGGCTTGCTGGTGCTCATCTCTGTCATCGACATCCTGGTGTCCATGGTCTCTGACAGC
  GGCACCAAGATTCTCGGCATGCTGAGGGTGCTGCGGCTGCTGCGGACCCTACGTCCACTCAGGGTCATCA
  GCCGGGCCCAGGGGCTGAAGCTGGTGGTAGAGACTCTGATGTCATCCCTCAAACCCATTGGCAACATTGT
  GGTCATCTGCTGTGCCTTCTTCATCATTTTTGGAATTCTTGGGGTGCAGCTCTTCAAAGGGAAGTTCTTC
  GTGTGTCAGGGTGAGGACACCAGGAACATCACTAACAAGTCCGACTGTGCTGAGGCCAGTTACCGGTGGG
  TCCGGCACAAGTACAACTTTGACAACCTGGGCCAGGCTCTGATGTCCCTGTTTGTGCTGGCCTCCAAGGA
  TGGCTGGGTTGACATCATGTATGATGGACTGGATGCTGTGGGAGTGGACCAGCAGCCCATCATGAACCAC
  AACCCTTGGATGCTGCTCTACTTCATCTCCTTCCTCCTCATCGTGGCCTTCTTCGTCCTGAACATGTTTG
  TGGGCGTGGTGGTGGAGAACTTCCATAAGTGCAGGCAGCACCAGGAGGAGGAGGAGGCGCGGCGGCGGGA
  GGAGAAGCGACTAAAGAGGCTGGAGAAAAAGAGAAGGAATCTAATGTTGGACGATGTAATTGCTTCCGGC
  AGCTCAGCCAGCGCTGCGTCAGAAGCCCAGTGCAAACCCTACTACTCTGACTACTCGCGCTTCCGGCTCC
  TCGTCCACCACCTGTGTACCAGCCACTACCTGGACCTCTTCATCACTGGTGTCATCGGGCTGAATGTGGT
  CACGATGGCCATGGAACATTACCAGCAGCCCCAGATCCTGGACGAGGCTCTGAAGATCTGCAACTACATC
  TTTACCGTCATCTTTGTCTTGGAGTCAGTATTCAAACTTGTGGCCTTCGGCTTCCGCCGGTTCTTCCAGG
  ACAGGTGGAACCAGCTGGACCTGGCTATTGTGCTTCTGTCCATCATGGGCATCACGCTGGAAGAGATTGA
  GGTCAATGCTTCACTGCCCATCAACCCCACCATCATCCGTATCATGAGGGTGCTCCGCATTGCTCGAGTT
  CTGAAGCTGTTGAAGATGGCTGTGGGCATGCGGGCACTGCTGGACACGGTGATGCAGGCCCTGCCCCAGG
  TGGGGAACCTGGGACTTCTCTTCATGCTATTATTTTTCATCTTTGCAGCTCTGGGCGTGGAGCTCTTTGG
  AGACCTGGAGTGTGATGAGACACACCCTTGTGAGGGCTTGGGCCGGCATGCCACCTTTAGGAACTTTGGT
  ATGGCCTTTCTGACCCTCTTCCGAGTCTCCACTGGTGACAACTGGAATGGTATTATGAAGGACACCCTCC
  GGGACTGTGACCAGGAGTCCACCTGCTACAACACCGTCATCTCACCCATCTACTTCGTGTCCTTCGTGCT
  GACGGCCCAGTTTGTGCTGGTCAACGTGGTCATAGCCGTGCTGATGAAGCACCTGGAAGAGAGCAACAAA
  GAGGCCAAGGAGGAGGCGGAGTTGGAGGCGGAGCTGGAGCTAGAGATGAAGACACTCAGCCCGCAGCCCC
  ACTCCCCGCTGGGCAGCCCCTTCCTCTGGCCTGGGGTGGAAGGTGTCAATAGCCCTGACAGCCCTAAGCC
  TGGGGCTCCACACACCACGGCCCACATTGGAGCAGCCTCTTCAGGCTTCTCCCTTGAGCACCCCACGATG
  GTACCTCACACTGAGGAGGGGCCAGTCCCCCTAGGACCAGACCTGCTGACTGTGAGGAAGTCTGGTGTCA
  GCCGGACACACTCTCTGCCCAATGACAGCTACATGTGCCGCAATGGGAGCACTGCCGAGAGATCCCTAGG
  ACACAGGGGCTGGGGGCTCCCCAAAGCCCAGTCAGGCTCCATCTTGTCTGTTCACTCCCAACCAGCAGAC
  ACCAGCTGCATCCTACAGCTTCCCAAAGATGCACACTATCTGCTCCAGCCTCATGGGGCTCCCACCTGGG
  GCGCCATCCCTAAACTACCCCCACCTGGCCGCTCCCCTCTGGCTCAGAGGCCTCTCAGGCGCCAGGCAGC
  AATAAGGACTGACTCCCTGGACGTGCAGGGCCTGGGTAGCCGGGAAGACCTGTTGTCAGAGGTGAGTGGG
  CCCTCCTGCCCTCTGACCCGCTCCTCATCCTTCTGGGGCGGGTCGAGCATCCAGGTGCAGCAGCGCTCCG
  GCAGCCAGAGCAAAGTCTCCAAGCACATCCGCCTGCCAGCCCCTTGCCCAGGCCTGGAACCCAGCTGGGC
  CAAGGACCCTCAAGAGACCAGAAGCAGCTTAGAGCTGGACACGGAGCTGAGCTGGATTTCAGGAGACCTC
  CTGCCCAGCAGTCAGGAAGAACCCCTGTCCCCACGGGACTTGAAAAAATGCTACAGTGTAGAGGCCCAGA
  GCTGCCGGCGCAGGCCTGGGTCCTGGCTAGACGAACAGAGGAGACACTCCATCGCTGTCAGCTGCCTGGA
  CAGCGGCTCCCAGCCCCGCCTATGTCCAAGCCCCTCAAGCCTCGGGGGCCAACCTCTTGGGGGCCCTGGG
  AGCCGGCCTAAGAAAAAACTCAGCCCACCCAGTATCTCTATAGACCCCCCGGAGAGCCAGGGCCCTCGGC
  CCCCATGCAGTCCTGGCGTCTGCCTCAGGAGGAGGGCGCCGGCCAGTGACTCGAAGGATCCCTCGGCCTC
  CAGCCCCCTTGACAGCACGGCTGCCTCACCCTCCCCAAAGAAAGATGCGCTGAGTCTCTCTGGTTTGTCT
  TCTGACCCAACAGACCTGGATCCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR212118 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MDEEEDGAGAEESGQPRSFTQLNDLSGAGGRQGPGSTEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRP
  RSWCLRTVCNPWFERVSMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALG
  IFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPM
  LGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLEPYYQTENEDESPFICSQPRENGMR
  SCRSVPTLRGEGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQV
  ITLEGWVDIMYFVMDAHSFYNFIYFILLIIFSETKQRESQLMREQRVRFLSNASTLASFSEPGSCYEELL
  KYLVYILRKAARRLAQVSRAVGVRAGLLSSPVARGGQEPQPSGSCSRSHRRLSVHHLVHHHHHHHHHYHL
  GNGTLRVPRASPEIQDRDANGSRWLMLPPPSTPTPSGGPPRGAESVHSFYHADCHLEPVRCQAPPPRSPS
  EASGRTVGSGKVYPTVHTSPPPEMLKDKALVEVAPSPGPPTLTSFNIPPGPFSSMHKLLETQSTGACHSS
  CKISSPCSKADSGACGPDSCPYCARTGAGEPESADHEMPDSDSEAVYEFTQDAQHSDLRDPHRRRRPSLG
  PDAEPSSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEELTNALEISNIVFTSLFA
  LEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMRVLKLVRFLPALQRQLVV
  LMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLLWAIVTVFQILTQEDWNK
  VLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEGDATKSESEPDFFSPSVDGDGDRKKRL
  ALVALGEHSELRKSLLPPLIIHTAATPMSLPKSSSTGVGEALGSGSRRTSSSGSAEPGTAHHEMKSPPSA
  RSSPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGQESQDEEESSEEDRASPAGSDHR
  HRGSLEREAKSSFDLPDTLQVPGLHRTASGRSSASEHQDCNGKSASGRLARTLRADDPPLDGDDGDDEGN
  LSKGERLRAWVRARLPACCRERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVLVIIFLNCITIAMERPK
  IDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLLVLISVIDILVSMVSDS
  GTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFFIIFGILGVQLFKGKFF
  VCQGEDTRNITNKSDCAEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMYDGLDAVGVDQQPIMNH
  NPWMLLYFISFLLIVAFFVLNMFVGVVVENFHKCRQHQEEEEARRREEKRLKRLEKKRRNLMLDDVIASG
  SSASAASEAQCKPYYSDYSRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYI
  FTVIFVLESVFKLVAFGFRRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARV
  LKLLKMAVGMRALLDTVMQALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFG
  MAFLTLFRVSTGDNWNGIMKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNK
  EAKEEAELEAELELEMKTLSPQPHSPLGSPFLWPGVEGVNSPDSPKPGAPHTTAHIGAASSGFSLEHPTM
  VPHTEEGPVPLGPDLLTVRKSGVSRTHSLPNDSYMCRNGSTAERSLGHRGWGLPKAQSGSILSVHSQPAD
  TSCILQLPKDAHYLLQPHGAPTWGAIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLSEVSG
  PSCPLTRSSSFWGGSSIQVQQRSGSQSKVSKHIRLPAPCPGLEPSWAKDPQETRSSLELDTELSWISGDL
  LPSSQEEPLSPRDLKKCYSVEAQSCRRRPGSWLDEQRRHSIAVSCLDSGSQPRLCPSPSSLGGQPLGGPG
  SRPKKKLSPPSISIDPPESQGPRPPCSPGVCLRRRAPASDSKDPSASSPLDSTAASPSPKKDALSLSGLS
  SDPTDLDP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001177890
• ORF Size: 6744 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001177890.1, NP_001171361.1
• RefSeq Size: 8097 bp
• RefSeq ORF: 6747 bp
• Locus ID: 12291
• Cytogenetics: 11 D
• MW: 248.9 kDa