Lrp5 (NM_008513) Mouse Tagged ORF Clone

CAT#: MR212054

Lrp5 (Myc-DDK-tagged) - Mouse low density lipoprotein receptor-related protein 5 (Lrp5)

Product Images

Circular map for MR212054

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Lrp5
• Synonyms: BMND1; HBM; LR3; LRP7; mKIAA4142; OPPG
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR212054 representing NM_008513
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAACGGCGCCGACCCGGGCCCCTCCGCCGCCGCCGCCGCCGCTGCTGCTGCTGGTGCTGTACTGCA
  GCTTGGTCCCCGCCGCGGCCTCACCGCTCCTGTTGTTTGCCAACCGCCGGGATGTGCGGCTAGTGGATGC
  CGGCGGAGTGAAGCTGGAGTCCACCATTGTGGCCAGTGGCCTGGAGGATGCAGCTGCTGTAGACTTCCAG
  TTCTCCAAGGGTGCTGTGTACTGGACAGATGTGAGCGAGGAGGCCATCAAACAGACCTACCTGAACCAGA
  CTGGAGCTGCTGCACAGAACATTGTCATCTCGGGCCTCGTGTCACCTGATGGCCTGGCCTGTGACTGGGT
  TGGCAAGAAGCTGTACTGGACGGACTCCGAGACCAACCGCATTGAGGTTGCCAACCTCAATGGGACGTCC
  CGTAAGGTTCTCTTCTGGCAGGACCTGGACCAGCCAAGGGCCATTGCCCTGGATCCTGCACATGGGTACA
  TGTACTGGACTGACTGGGGGGAAGCACCCCGGATCGAGCGGGCAGGGATGGATGGCAGTACCCGGAAGAT
  CATTGTAGACTCCGACATTTACTGGCCCAATGGGCTGACCATCGACCTGGAGGAACAGAAGCTGTACTGG
  GCCGATGCCAAGCTCAGCTTCATCCACCGTGCCAACCTGGACGGCTCCTTCCGGCAGAAGGTGGTGGAGG
  GCAGCCTCACTCACCCTTTTGCCCTGACACTCTCTGGGGACACACTCTACTGGACAGACTGGCAGACCCG
  CTCCATCCACGCCTGCAACAAGTGGACAGGGGAGCAGAGGAAGGAGATCCTTAGTGCTCTGTACTCACCC
  ATGGACATCCAAGTGCTGAGCCAGGAGCGGCAGCCTCCCTTCCACACACCATGCGAGGAGGACAACGGTG
  GCTGTTCCCACCTGTGCCTGCTGTCCCCGAGGGAGCCTTTCTACTCCTGTGCCTGCCCCACTGGTGTGCA
  GTTGCAGGACAATGGCAAGACGTGCAAGACAGGGGCTGAGGAAGTGCTGCTGCTGGCTCGGAGGACAGAC
  CTGAGGAGGATCTCTCTGGACACCCCTGACTTCACAGACATAGTGCTGCAGGTGGGCGACATCCGGCATG
  CCATTGCCATTGACTACGATCCCCTGGAGGGCTACGTGTACTGGACCGACGATGAGGTGCGGGCTATCCG
  CAGGGCGTACCTAGATGGCTCAGGTGCGCAGACACTTGTGAACACTGAGATCAATGACCCCGATGGCATT
  GCTGTGGACTGGGTCGCCCGGAACCTCTACTGGACAGATACAGGCACTGACAGAATTGAGGTGACTCGCC
  TCAACGGCACCTCCCGAAAGATCCTGGTATCTGAGGACCTGGACGAACCGCGAGCCATTGTGTTGCACCC
  TGTGATGGGCCTCATGTACTGGACAGACTGGGGGGAGAACCCCAAAATCGAATGCGCCAACCTAGATGGG
  AGAGATCGGCATGTCCTGGTGAACACCTCCCTTGGGTGGCCCAACGGACTGGCCCTGGACCTGCAGGAGG
  GCAAGCTGTACTGGGGGGATGCCAAAACTGATAAAATCGAGGTGATCAACATAGATGGGACAAAGCGGAA
  GACCCTGCTTGAGGACAAGCTCCCACATATTTTTGGGTTCACACTGCTGGGGGACTTCATCTACTGGACC
  GACTGGCAGAGACGCAGTATTGAAAGGGTCCACAAGGTCAAGGCCAGCCGGGATGTCATCATTGATCAAC
  TCCCCGACCTGATGGGACTCAAAGCTGTGAATGTGGCCAAGGTTGTCGGAACCAACCCATGTGCGGATGG
  AAATGGAGGGTGCAGCCATCTGTGCTTCTTCACCCCACGTGCCACCAAGTGTGGCTGCCCCATTGGCCTG
  GAGCTGTTGAGTGACATGAAGACCTGCATAATCCCCGAGGCCTTCCTGGTATTCACCAGCAGAGCCACCA
  TCCACAGGATCTCCCTGGAGACTAACAACAACGATGTGGCTATCCCACTCACGGGTGTCAAAGAGGCCTC
  TGCACTGGACTTTGATGTGTCCAACAATCACATCTACTGGACTGATGTCAGCCTCAAGACGATCAGCCGA
  GCCTTCATGAATGGGAGCTCAGTGGAGCACGTGATTGAGTTTGGCCTCGACTACCCTGAAGGAATGGCTG
  TGGACTGGATGGGCAAGAACCTCTATTGGGCGGACACAGGGACCAACAGGATTGAGGTGGCCCGGCTGGA
  TGGGCAGTTCCGGCAGGTGCTTGTGTGGAGAGACCTTGACAACCCCAGGTCTCTGGCTCTGGATCCTACT
  AAAGGCTACATCTACTGGACTGAGTGGGGTGGCAAGCCAAGGATTGTGCGGGCCTTCATGGATGGGACCA
  ATTGTATGACACTGGTAGACAAGGTGGGCCGGGCCAACGACCTCACCATTGATTATGCCGACCAGCGACT
  GTACTGGACTGACCTGGACACCAACATGATTGAGTCTTCCAACATGCTGGGTCAGGAGCGCATGGTGATA
  GCTGACGATCTGCCCTACCCGTTTGGCCTGACTCAATATAGCGATTACATCTACTGGACTGACTGGAACC
  TGCATAGCATTGAACGGGCGGACAAGACCAGTGGGCGGAACCGCACCCTCATCCAGGGTCACCTGGACTT
  CGTCATGGACATCCTGGTGTTCCACTCCTCCCGTCAGGATGGCCTCAACGACTGCGTGCACAGCAATGGC
  CAGTGTGGGCAGCTGTGCCTCGCCATCCCCGGAGGCCACCGCTGTGGCTGTGCTTCACACTACACGCTGG
  ACCCCAGCAGCCGCAACTGCAGCCCGCCCTCCACCTTCTTGCTGTTCAGCCAGAAATTTGCCATCAGCCG
  GATGATCCCCGATGACCAGCTCAGCCCGGACCTTGTCCTACCCCTTCATGGGCTGAGGAACGTCAAAGCC
  ATCAACTATGACCCGCTGGACAAGTTCATCTACTGGGTGGACGGGCGCCAGAACATCAAGAGGGCCAAGG
  ACGACGGTACCCAGCCCTCCATGCTGACCTCTCCCAGCCAAAGCCTGAGCCCAGACAGACAGCCACACGA
  CCTCAGCATTGACATCTACAGCCGGACACTGTTCTGGACCTGTGAGGCCACCAACACTATCAATGTCCAC
  CGGCTGGATGGGGATGCCATGGGAGTGGTGCTTCGAGGGGACCGTGACAAGCCAAGGGCCATTGCTGTCA
  ATGCTGAGCGAGGGTACATGTACTTTACCAACATGCAGGACCATGCTGCCAAGATCGAGCGAGCCTCCCT
  GGATGGCACAGAGCGGGAGGTCCTCTTCACCACAGGCCTCATCCGTCCCGTGGCCCTTGTGGTGGACAAT
  GCTCTGGGCAAGCTCTTCTGGGTGGATGCCGACCTAAAGCGAATCGAAAGCTGTGACCTCTCTGGGGCCA
  ACCGCCTGACCCTGGAAGATGCCAACATCGTACAGCCAGTAGGTCTGACAGTGCTGGGCAGGCACCTCTA
  CTGGATCGACCGCCAGCAGCAGATGATCGAGCGCGTGGAGAAGACCACTGGGGACAAGCGGACTAGGGTT
  CAGGGCCGTGTCACCCACCTGACAGGCATCCATGCCGTGGAGGAAGTCAGCCTGGAGGAGTTCTCAGCCC
  ATCCTTGTGCCCGAGACAATGGCGGCTGCTCCCACATCTGTATCGCCAAGGGTGATGGAACACCGCGCTG
  CTCGTGCCCTGTCCACCTGGTGCTCCTGCAGAACCTGCTGACTTGTGGTGAGCCTCCTACCTGCTCCCCT
  GATCAGTTTGCATGTACCACTGGTGAGATCGACTGCATCCCCGGAGCCTGGCGCTGTGACGGCTTCCCTG
  AGTGTGCTGACCAGAGTGATGAAGAAGGCTGCCCAGTGTGCTCCGCCTCTCAGTTCCCCTGCGCTCGAGG
  CCAGTGTGTGGACCTGCGGTTACGCTGCGACGGTGAGGCCGACTGCCAGGATCGCTCTGATGAAGCTAAC
  TGCGATGCTGTCTGTCTGCCCAATCAGTTCCGGTGCACCAGCGGCCAGTGTGTCCTCATCAAGCAACAGT
  GTGACTCCTTCCCCGACTGTGCTGATGGGTCTGATGAGCTCATGTGTGAAATCAACAAGCCACCCTCTGA
  TGACATCCCAGCCCACAGCAGTGCCATTGGGCCCGTCATTGGTATCATCCTCTCCCTCTTCGTCATGGGC
  GGGGTCTACTTTGTCTGCCAGCGTGTGATGTGCCAGCGCTACACAGGGGCCAGTGGGCCCTTTCCCCACG
  AGTATGTTGGTGGAGCCCCTCATGTGCCTCTCAACTTCATAGCCCCAGGTGGCTCACAGCACGGTCCCTT
  CCCAGGCATCCCGTGCAGCAAGTCCGTGATGAGCTCCATGAGCCTGGTGGGGGGGCGCGGCAGTGTGCCC
  CTCTATGACCGGAATCACGTCACTGGGGCCTCATCCAGCAGCTCGTCCAGCACAAAGGCCACACTATATC
  CGCCGATCCTGAACCCACCCCCGTCCCCGGCCACAGACCCCTCTCTCTACAACGTGGACGTGTTTTATTC
  TTCAGGCATCCCGGCCACCGCTAGACCATACAGGCCCTACGTCATTCGAGGTATGGCACCCCCAACAACA
  CCGTGCAGCACAGATGTGTGTGACAGTGACTACAGCACCAGTCGCTGGAAGAGCAGCAAATACTACCTGG
  ACTTGAATTCGGACTCAGACCCCTACCCCCCCCCGCCCACCCCCCACAGCCAGTACCTATCTGCAGAGGA
  CAGCTGCCCACCCTCACCAGGCACTGAGAGGAGTTACTGCCACCTCTTCCCGCCCCCACCGTCCCCCTGC
  ACGGACTCGTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR212054 representing NM_008513
  Red=Cloning site Green=Tags(s)
  
  METAPTRAPPPPPPPLLLLVLYCSLVPAAASPLLLFANRRDVRLVDAGGVKLESTIVASGLEDAAAVDFQ
  FSKGAVYWTDVSEEAIKQTYLNQTGAAAQNIVISGLVSPDGLACDWVGKKLYWTDSETNRIEVANLNGTS
  RKVLFWQDLDQPRAIALDPAHGYMYWTDWGEAPRIERAGMDGSTRKIIVDSDIYWPNGLTIDLEEQKLYW
  ADAKLSFIHRANLDGSFRQKVVEGSLTHPFALTLSGDTLYWTDWQTRSIHACNKWTGEQRKEILSALYSP
  MDIQVLSQERQPPFHTPCEEDNGGCSHLCLLSPREPFYSCACPTGVQLQDNGKTCKTGAEEVLLLARRTD
  LRRISLDTPDFTDIVLQVGDIRHAIAIDYDPLEGYVYWTDDEVRAIRRAYLDGSGAQTLVNTEINDPDGI
  AVDWVARNLYWTDTGTDRIEVTRLNGTSRKILVSEDLDEPRAIVLHPVMGLMYWTDWGENPKIECANLDG
  RDRHVLVNTSLGWPNGLALDLQEGKLYWGDAKTDKIEVINIDGTKRKTLLEDKLPHIFGFTLLGDFIYWT
  DWQRRSIERVHKVKASRDVIIDQLPDLMGLKAVNVAKVVGTNPCADGNGGCSHLCFFTPRATKCGCPIGL
  ELLSDMKTCIIPEAFLVFTSRATIHRISLETNNNDVAIPLTGVKEASALDFDVSNNHIYWTDVSLKTISR
  AFMNGSSVEHVIEFGLDYPEGMAVDWMGKNLYWADTGTNRIEVARLDGQFRQVLVWRDLDNPRSLALDPT
  KGYIYWTEWGGKPRIVRAFMDGTNCMTLVDKVGRANDLTIDYADQRLYWTDLDTNMIESSNMLGQERMVI
  ADDLPYPFGLTQYSDYIYWTDWNLHSIERADKTSGRNRTLIQGHLDFVMDILVFHSSRQDGLNDCVHSNG
  QCGQLCLAIPGGHRCGCASHYTLDPSSRNCSPPSTFLLFSQKFAISRMIPDDQLSPDLVLPLHGLRNVKA
  INYDPLDKFIYWVDGRQNIKRAKDDGTQPSMLTSPSQSLSPDRQPHDLSIDIYSRTLFWTCEATNTINVH
  RLDGDAMGVVLRGDRDKPRAIAVNAERGYMYFTNMQDHAAKIERASLDGTEREVLFTTGLIRPVALVVDN
  ALGKLFWVDADLKRIESCDLSGANRLTLEDANIVQPVGLTVLGRHLYWIDRQQQMIERVEKTTGDKRTRV
  QGRVTHLTGIHAVEEVSLEEFSAHPCARDNGGCSHICIAKGDGTPRCSCPVHLVLLQNLLTCGEPPTCSP
  DQFACTTGEIDCIPGAWRCDGFPECADQSDEEGCPVCSASQFPCARGQCVDLRLRCDGEADCQDRSDEAN
  CDAVCLPNQFRCTSGQCVLIKQQCDSFPDCADGSDELMCEINKPPSDDIPAHSSAIGPVIGIILSLFVMG
  GVYFVCQRVMCQRYTGASGPFPHEYVGGAPHVPLNFIAPGGSQHGPFPGIPCSKSVMSSMSLVGGRGSVP
  LYDRNHVTGASSSSSSSTKATLYPPILNPPPSPATDPSLYNVDVFYSSGIPATARPYRPYVIRGMAPPTT
  PCSTDVCDSDYSTSRWKSSKYYLDLNSDSDPYPPPPTPHSQYLSAEDSCPPSPGTERSYCHLFPPPPSPC
  TDSS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008513
• ORF Size: 4842 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008513.3, NP_032539.2
• RefSeq Size: 5172 bp
• RefSeq ORF: 4845 bp
• Locus ID: 16973
• UniProt ID: Q91VN0
• Cytogenetics: 19 A
• MW: 179.3 kDa
• Gene Summary: Acts as a coreceptor with members of the frizzled family of seven-transmembrane spanning receptors to transduce signal by Wnt proteins. Activates the canonical Wnt signaling pathway that controls cell fate determination and self-renewal during embryonic development and adult tissue regeneration (PubMed:11956231). In particular, may play an important role in the development of the posterior patterning of the epiblast during gastrulation (PubMed:15142971). During bone development, regulates osteoblast proliferation and differentiation thus determining bone mass (PubMed:11956231). Mechanistically, the formation of the signaling complex between Wnt ligand, frizzled receptor and LRP5 coreceptor promotes the recruitment of AXIN1 to LRP5, stabilizing beta-catenin/CTNNB1 and activating TCF/LEF-mediated transcriptional programs (By similarity). Acts as a coreceptor for non-Wnt proteins, such as norrin/NDP. Binding of norrin/NDP to frizzled 4/FZD4-LRP5 receptor complex triggers beta-catenin/CTNNB1-dependent signaling known to be required for retinal vascular development (By similarity). Plays a role in controlling postnatal vascular regression in retina via macrophage-induced endothelial cell apoptosis (PubMed:11956231).[UniProtKB/Swiss-Prot Function]